Mutagenetix > Incidental Mutation

Incidental Mutation 'R7171:Hectd1'

558294

Institutional Source

Beutler Lab

Gene Symbol

Hectd1

Ensembl Gene

ENSMUSG00000035247

Gene Name

HECT domain E3 ubiquitin protein ligase 1

Synonyms

A630086P08Rik, b2b327Clo, opm

MMRRC Submission

045230-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R7171 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

51790505-51876319 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 51806080 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Valine to Glutamic Acid at position 1852 (V1852E)

Ref Sequence

ENSEMBL: ENSMUSP00000136449 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000042052] [ENSMUST00000179265]

AlphaFold

Q69ZR2

Predicted Effect

probably damaging
Transcript: ENSMUST00000042052
AA Change: V1847E

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000046766
Gene: ENSMUSG00000035247
AA Change: V1847E

Domain	Start	End	E-Value	Type
low complexity region	317	331	N/A	INTRINSIC
ANK	395	424	1.44e-1	SMART
ANK	426	455	2.81e-4	SMART
ANK	459	488	1.55e2	SMART
low complexity region	490	509	N/A	INTRINSIC
low complexity region	630	654	N/A	INTRINSIC
low complexity region	707	723	N/A	INTRINSIC
low complexity region	821	832	N/A	INTRINSIC
Pfam:Sad1_UNC	1107	1240	9.2e-27	PFAM
low complexity region	1259	1271	N/A	INTRINSIC
Pfam:MIB_HERC2	1277	1338	7.6e-27	PFAM
low complexity region	1373	1401	N/A	INTRINSIC
low complexity region	1441	1458	N/A	INTRINSIC
low complexity region	1484	1495	N/A	INTRINSIC
low complexity region	1508	1524	N/A	INTRINSIC
low complexity region	1600	1630	N/A	INTRINSIC
low complexity region	1633	1651	N/A	INTRINSIC
low complexity region	1674	1703	N/A	INTRINSIC
low complexity region	1745	1752	N/A	INTRINSIC
PDB:2LC3\|A	1879	1966	4e-57	PDB
low complexity region	2101	2117	N/A	INTRINSIC
HECTc	2143	2610	8.32e-76	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000179265
AA Change: V1852E

PolyPhen 2 Score 0.993 (Sensitivity: 0.70; Specificity: 0.97)

SMART Domains

Protein: ENSMUSP00000136449
Gene: ENSMUSG00000035247
AA Change: V1852E

Domain	Start	End	E-Value	Type
low complexity region	317	331	N/A	INTRINSIC
ANK	396	425	1.44e-1	SMART
ANK	427	456	2.81e-4	SMART
ANK	460	489	1.55e2	SMART
low complexity region	491	510	N/A	INTRINSIC
low complexity region	631	655	N/A	INTRINSIC
low complexity region	708	724	N/A	INTRINSIC
low complexity region	822	833	N/A	INTRINSIC
Pfam:Sad1_UNC	1112	1245	1.3e-26	PFAM
low complexity region	1264	1276	N/A	INTRINSIC
Pfam:MIB_HERC2	1282	1341	5.3e-26	PFAM
low complexity region	1378	1406	N/A	INTRINSIC
low complexity region	1446	1463	N/A	INTRINSIC
low complexity region	1489	1500	N/A	INTRINSIC
low complexity region	1513	1529	N/A	INTRINSIC
low complexity region	1605	1635	N/A	INTRINSIC
low complexity region	1638	1656	N/A	INTRINSIC
low complexity region	1679	1708	N/A	INTRINSIC
low complexity region	1750	1757	N/A	INTRINSIC
PDB:2LC3\|A	1884	1971	3e-57	PDB
low complexity region	2106	2122	N/A	INTRINSIC
HECTc	2148	2618	4.5e-72	SMART

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.7%
20x: 98.9%

Validation Efficiency

100% (81/81)

MGI Phenotype

PHENOTYPE: Mice that are homozygous for either a gene trapped or an ENU-induced allele exhibit exencephaly associated with impaired head mesenchyme development and neural tube closure, and show eye and cranial vault dysplasia. Homozygotes for another ENU-induced allele show congenital cardiovascular defects. [provided by MGI curators]

Allele List at MGI

All alleles(30) : Gene trapped(29) Chemically induced(1)

Other mutations in this stock

Total: 81 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930519P11Rik	T	C	2: 154,454,897 (GRCm39)	N154S	unknown	Het
Ank3	C	T	10: 69,828,311 (GRCm39)	H2327Y		Het
Ano6	A	G	15: 95,818,172 (GRCm39)	Y305C	probably damaging	Het
Apc2	A	G	10: 80,151,170 (GRCm39)	T2075A	possibly damaging	Het
Arl6	A	G	16: 59,443,455 (GRCm39)	V93A	possibly damaging	Het
Bcas3	T	C	11: 85,474,763 (GRCm39)	S790P	probably damaging	Het
Bcl9l	C	A	9: 44,416,448 (GRCm39)	H211N	probably benign	Het
Bptf	G	T	11: 107,022,233 (GRCm39)	D172E	unknown	Het
Card9	T	G	2: 26,249,496 (GRCm39)	I22L	possibly damaging	Het
Catsperg1	C	A	7: 28,884,637 (GRCm39)	G933V	probably damaging	Het
Catsperg2	T	A	7: 29,404,750 (GRCm39)	H771L	possibly damaging	Het
Ccdc66	T	C	14: 27,215,229 (GRCm39)	N281S	possibly damaging	Het
Cfap54	A	T	10: 92,612,072 (GRCm39)	L3162H	probably damaging	Het
Clca4a	A	G	3: 144,663,934 (GRCm39)	L503P	probably benign	Het
Cpn1	T	C	19: 43,962,470 (GRCm39)	N160D	probably damaging	Het
Ctsh	T	A	9: 89,949,154 (GRCm39)	I206K	probably benign	Het
Cyb561d1	G	A	3: 108,106,679 (GRCm39)	T180M	probably damaging	Het
Dapk1	A	T	13: 60,909,599 (GRCm39)	D1404V	probably damaging	Het
Dip2c	G	T	13: 9,556,684 (GRCm39)	R76L	probably benign	Het
Dnah7c	T	C	1: 46,719,898 (GRCm39)	I2783T	probably damaging	Het
E130308A19Rik	T	C	4: 59,690,333 (GRCm39)	Y56H	probably damaging	Het
Efemp2	G	T	19: 5,530,285 (GRCm39)	A310S	probably benign	Het
Enpp5	G	A	17: 44,396,155 (GRCm39)	G356S	probably damaging	Het
Faap24	G	A	7: 35,092,279 (GRCm39)	Q213*	probably null	Het
Fam181b	G	A	7: 92,729,943 (GRCm39)	G239S	possibly damaging	Het
Fancm	T	C	12: 65,148,394 (GRCm39)	W670R	probably damaging	Het
Fcgbpl1	T	A	7: 27,853,944 (GRCm39)	L1636*	probably null	Het
Fmnl1	T	C	11: 103,081,224 (GRCm39)	Y357H	probably damaging	Het
Fryl	A	T	5: 73,279,653 (GRCm39)	V215E	probably damaging	Het
Fscn2	C	A	11: 120,253,335 (GRCm39)	N267K	probably damaging	Het
Fsip2	T	A	2: 82,816,571 (GRCm39)	Y4101*	probably null	Het
Gcn1	G	A	5: 115,728,352 (GRCm39)	V738I	probably benign	Het
Gdf9	C	T	11: 53,328,366 (GRCm39)	R441C	probably damaging	Het
Gipc3	C	T	10: 81,177,455 (GRCm39)	G79E	probably damaging	Het
Gzmf	T	A	14: 56,443,391 (GRCm39)	I196L	probably benign	Het
Hip1r	A	T	5: 124,134,007 (GRCm39)	Q256L	probably benign	Het
Hmgxb4	T	C	8: 75,746,890 (GRCm39)	S405P	probably damaging	Het
Il9r	T	A	11: 32,142,671 (GRCm39)	H294L	probably benign	Het
Kif12	G	A	4: 63,086,931 (GRCm39)	T331I	probably damaging	Het
Krtap8-1	A	T	16: 89,284,794 (GRCm39)	M1K	probably null	Het
Lamc2	A	T	1: 153,015,495 (GRCm39)	C613S	probably damaging	Het
Lgr4	T	A	2: 109,831,314 (GRCm39)	N314K	probably benign	Het
Lpcat2	T	C	8: 93,635,894 (GRCm39)	I432T	probably benign	Het
Mfhas1	T	C	8: 36,056,146 (GRCm39)	V207A	probably benign	Het
Msh3	T	G	13: 92,485,806 (GRCm39)	T173P	probably benign	Het
Mup15	T	A	4: 61,356,505 (GRCm39)	M87L	probably benign	Het
N4bp2	T	C	5: 65,965,365 (GRCm39)	V1138A	probably benign	Het
Noc2l	T	C	4: 156,326,179 (GRCm39)	V422A	probably benign	Het
Notch3	C	T	17: 32,377,936 (GRCm39)	G74D	probably damaging	Het
Ocstamp	T	A	2: 165,240,081 (GRCm39)	K35I	probably benign	Het
Or12k7	T	C	2: 36,958,400 (GRCm39)	F28L	possibly damaging	Het
Or52w1	A	G	7: 105,017,968 (GRCm39)	H145R	probably benign	Het
Or5p58	A	G	7: 107,694,342 (GRCm39)	V145A	probably benign	Het
Or9a2	T	G	6: 41,748,961 (GRCm39)	T91P	probably benign	Het
Plekhh2	T	A	17: 84,829,216 (GRCm39)	V29D	probably damaging	Het
Pof1b	A	G	X: 111,554,042 (GRCm39)	I544T	probably benign	Het
Rad54l2	C	T	9: 106,590,677 (GRCm39)	R483H	probably damaging	Het
Rapgef6	T	A	11: 54,567,189 (GRCm39)	D1128E	possibly damaging	Het
Rhbdl2	T	A	4: 123,708,049 (GRCm39)	I86N	possibly damaging	Het
Rims1	C	A	1: 22,498,740 (GRCm39)	R68L		Het
Rnf157	A	G	11: 116,253,199 (GRCm39)	F70L	possibly damaging	Het
Rtp3	C	T	9: 110,815,009 (GRCm39)	C452Y	unknown	Het
S100a14	A	T	3: 90,435,069 (GRCm39)	K27*	probably null	Het
Scai	C	T	2: 38,996,948 (GRCm39)	G282D	possibly damaging	Het
Sclt1	A	T	3: 41,672,195 (GRCm39)	I132N	probably benign	Het
Serpinf1	T	A	11: 75,308,811 (GRCm39)	Q2L	possibly damaging	Het
Skor2	T	A	18: 76,948,681 (GRCm39)	V801E	probably benign	Het
Slc16a10	C	G	10: 39,913,255 (GRCm39)	E484D	probably benign	Het
Slc1a5	T	A	7: 16,531,463 (GRCm39)	D489E	probably damaging	Het
Slx4	C	T	16: 3,808,650 (GRCm39)	R430H	probably benign	Het
Teddm1b	A	T	1: 153,750,679 (GRCm39)	I163F	probably damaging	Het
Tmem171	A	T	13: 98,828,744 (GRCm39)	C135*	probably null	Het
Tmem82	C	T	4: 141,342,284 (GRCm39)	R306H	possibly damaging	Het
Tmem94	T	A	11: 115,681,781 (GRCm39)		probably null	Het
Trpm2	A	C	10: 77,759,848 (GRCm39)	L1096R	probably damaging	Het
Trrap	T	A	5: 144,730,859 (GRCm39)	L780Q	probably damaging	Het
Txlnb	A	G	10: 17,718,732 (GRCm39)	D521G	probably benign	Het
Usp7	T	C	16: 8,534,390 (GRCm39)	D59G	probably benign	Het
Vcan	G	A	13: 89,873,710 (GRCm39)	T48I	probably damaging	Het
Vmn1r45	A	G	6: 89,910,316 (GRCm39)	L218P	probably damaging	Het
Wdr25	A	T	12: 108,990,922 (GRCm39)	T370S	probably damaging	Het

Other mutations in Hectd1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00402:Hectd1	APN	12	51,806,215 (GRCm39)	missense	probably benign
IGL00402:Hectd1	APN	12	51,815,891 (GRCm39)	missense	possibly damaging	0.94
IGL00419:Hectd1	APN	12	51,810,818 (GRCm39)	missense	probably damaging	0.99
IGL00518:Hectd1	APN	12	51,823,272 (GRCm39)	splice site	probably benign
IGL00565:Hectd1	APN	12	51,837,181 (GRCm39)	missense	probably damaging	0.97
IGL00574:Hectd1	APN	12	51,820,787 (GRCm39)	missense	probably benign	0.17
IGL00576:Hectd1	APN	12	51,806,092 (GRCm39)	missense	probably damaging	0.99
IGL00788:Hectd1	APN	12	51,795,571 (GRCm39)	missense	probably damaging	0.99
IGL00978:Hectd1	APN	12	51,838,173 (GRCm39)	missense	possibly damaging	0.95
IGL01328:Hectd1	APN	12	51,807,904 (GRCm39)	missense	probably damaging	1.00
IGL01337:Hectd1	APN	12	51,849,057 (GRCm39)	missense	possibly damaging	0.95
IGL01634:Hectd1	APN	12	51,850,562 (GRCm39)	missense	probably damaging	0.98
IGL01731:Hectd1	APN	12	51,849,593 (GRCm39)	missense	possibly damaging	0.59
IGL01920:Hectd1	APN	12	51,829,337 (GRCm39)	missense	probably damaging	0.99
IGL01951:Hectd1	APN	12	51,841,280 (GRCm39)	nonsense	probably null
IGL01994:Hectd1	APN	12	51,844,725 (GRCm39)	missense	probably damaging	0.99
IGL02140:Hectd1	APN	12	51,820,920 (GRCm39)	missense	probably damaging	0.99
IGL02150:Hectd1	APN	12	51,815,974 (GRCm39)	missense	probably damaging	0.97
IGL02156:Hectd1	APN	12	51,800,916 (GRCm39)	splice site	probably benign
IGL02177:Hectd1	APN	12	51,819,103 (GRCm39)	missense	probably damaging	0.99
IGL02502:Hectd1	APN	12	51,844,635 (GRCm39)	missense	possibly damaging	0.77
IGL02505:Hectd1	APN	12	51,847,496 (GRCm39)	critical splice donor site	probably null
IGL02519:Hectd1	APN	12	51,815,894 (GRCm39)	missense	probably damaging	0.99
IGL02624:Hectd1	APN	12	51,809,233 (GRCm39)	missense	possibly damaging	0.61
IGL02833:Hectd1	APN	12	51,810,864 (GRCm39)	missense	probably damaging	0.96
IGL02851:Hectd1	APN	12	51,814,423 (GRCm39)	missense	possibly damaging	0.94
IGL02866:Hectd1	APN	12	51,837,396 (GRCm39)	missense	probably damaging	1.00
IGL02981:Hectd1	APN	12	51,815,670 (GRCm39)	missense	possibly damaging	0.70
IGL02987:Hectd1	APN	12	51,791,550 (GRCm39)	missense	probably damaging	1.00
IGL02999:Hectd1	APN	12	51,874,205 (GRCm39)	missense	possibly damaging	0.77
IGL03071:Hectd1	APN	12	51,815,957 (GRCm39)	missense	probably benign	0.00
IGL03078:Hectd1	APN	12	51,849,019 (GRCm39)	missense	probably damaging	0.98
IGL03299:Hectd1	APN	12	51,847,671 (GRCm39)	splice site	probably benign
3-1:Hectd1	UTSW	12	51,800,590 (GRCm39)	missense	probably damaging	0.99
R0039:Hectd1	UTSW	12	51,800,608 (GRCm39)	missense	possibly damaging	0.83
R0238:Hectd1	UTSW	12	51,816,101 (GRCm39)	missense	possibly damaging	0.72
R0238:Hectd1	UTSW	12	51,816,101 (GRCm39)	missense	possibly damaging	0.72
R0239:Hectd1	UTSW	12	51,816,101 (GRCm39)	missense	possibly damaging	0.72
R0239:Hectd1	UTSW	12	51,816,101 (GRCm39)	missense	possibly damaging	0.72
R0268:Hectd1	UTSW	12	51,815,891 (GRCm39)	missense	possibly damaging	0.94
R0268:Hectd1	UTSW	12	51,815,890 (GRCm39)	missense	probably damaging	0.99
R0409:Hectd1	UTSW	12	51,829,339 (GRCm39)	missense	possibly damaging	0.59
R1019:Hectd1	UTSW	12	51,795,440 (GRCm39)	missense	probably damaging	0.99
R1072:Hectd1	UTSW	12	51,807,855 (GRCm39)	missense	probably benign	0.11
R1087:Hectd1	UTSW	12	51,823,355 (GRCm39)	missense	probably damaging	0.99
R1165:Hectd1	UTSW	12	51,810,947 (GRCm39)	splice site	probably benign
R1350:Hectd1	UTSW	12	51,809,217 (GRCm39)	missense	probably benign
R1553:Hectd1	UTSW	12	51,820,661 (GRCm39)	missense	probably damaging	0.98
R1666:Hectd1	UTSW	12	51,800,607 (GRCm39)	missense	possibly damaging	0.91
R1676:Hectd1	UTSW	12	51,791,571 (GRCm39)	missense	probably damaging	1.00
R1694:Hectd1	UTSW	12	51,791,375 (GRCm39)	missense	probably damaging	1.00
R1778:Hectd1	UTSW	12	51,800,590 (GRCm39)	missense	probably damaging	0.99
R1856:Hectd1	UTSW	12	51,791,577 (GRCm39)	missense	probably damaging	1.00
R1859:Hectd1	UTSW	12	51,853,350 (GRCm39)	missense	probably damaging	1.00
R1884:Hectd1	UTSW	12	51,847,738 (GRCm39)	missense	probably benign	0.00
R1982:Hectd1	UTSW	12	51,832,624 (GRCm39)	missense	probably damaging	0.97
R2034:Hectd1	UTSW	12	51,803,899 (GRCm39)	splice site	probably null
R2061:Hectd1	UTSW	12	51,841,227 (GRCm39)	missense	probably damaging	0.99
R2078:Hectd1	UTSW	12	51,795,325 (GRCm39)	missense	probably damaging	0.99
R2176:Hectd1	UTSW	12	51,792,277 (GRCm39)	missense	probably damaging	1.00
R2210:Hectd1	UTSW	12	51,853,245 (GRCm39)	missense	probably damaging	0.99
R2248:Hectd1	UTSW	12	51,853,254 (GRCm39)	missense	probably damaging	0.99
R2282:Hectd1	UTSW	12	51,815,791 (GRCm39)	missense	possibly damaging	0.95
R2402:Hectd1	UTSW	12	51,792,317 (GRCm39)	missense	probably benign	0.01
R3876:Hectd1	UTSW	12	51,815,513 (GRCm39)	missense	probably damaging	0.98
R4027:Hectd1	UTSW	12	51,849,219 (GRCm39)	critical splice acceptor site	probably null
R4085:Hectd1	UTSW	12	51,821,533 (GRCm39)	missense	possibly damaging	0.93
R4115:Hectd1	UTSW	12	51,815,506 (GRCm39)	nonsense	probably null
R4116:Hectd1	UTSW	12	51,815,506 (GRCm39)	nonsense	probably null
R4169:Hectd1	UTSW	12	51,837,008 (GRCm39)	missense	probably damaging	0.97
R4434:Hectd1	UTSW	12	51,798,835 (GRCm39)	missense	probably damaging	1.00
R4507:Hectd1	UTSW	12	51,837,276 (GRCm39)	missense	probably damaging	0.97
R4578:Hectd1	UTSW	12	51,798,715 (GRCm39)	missense	probably damaging	1.00
R4579:Hectd1	UTSW	12	51,791,356 (GRCm39)	missense	probably damaging	0.97
R4709:Hectd1	UTSW	12	51,834,695 (GRCm39)	missense	possibly damaging	0.94
R4812:Hectd1	UTSW	12	51,874,134 (GRCm39)	critical splice donor site	probably null
R4883:Hectd1	UTSW	12	51,831,030 (GRCm39)	nonsense	probably null
R4885:Hectd1	UTSW	12	51,847,505 (GRCm39)	missense	probably damaging	0.97
R4975:Hectd1	UTSW	12	51,809,280 (GRCm39)	missense	probably benign	0.02
R4983:Hectd1	UTSW	12	51,831,045 (GRCm39)	missense	probably benign	0.01
R5007:Hectd1	UTSW	12	51,849,443 (GRCm39)	missense	possibly damaging	0.95
R5046:Hectd1	UTSW	12	51,797,171 (GRCm39)	missense	probably damaging	1.00
R5062:Hectd1	UTSW	12	51,791,662 (GRCm39)	missense	probably damaging	0.98
R5164:Hectd1	UTSW	12	51,874,272 (GRCm39)	start codon destroyed	probably null	0.60
R5213:Hectd1	UTSW	12	51,849,316 (GRCm39)	critical splice donor site	probably null
R5535:Hectd1	UTSW	12	51,849,109 (GRCm39)	missense	probably damaging	0.98
R5776:Hectd1	UTSW	12	51,810,897 (GRCm39)	missense	possibly damaging	0.91
R5846:Hectd1	UTSW	12	51,820,618 (GRCm39)	missense	probably damaging	0.99
R5907:Hectd1	UTSW	12	51,845,537 (GRCm39)	missense	probably damaging	0.98
R5911:Hectd1	UTSW	12	51,849,035 (GRCm39)	missense	probably damaging	0.99
R5919:Hectd1	UTSW	12	51,815,855 (GRCm39)	missense	probably damaging	0.98
R6051:Hectd1	UTSW	12	51,800,887 (GRCm39)	missense	probably benign
R6141:Hectd1	UTSW	12	51,792,875 (GRCm39)	critical splice donor site	probably null
R6172:Hectd1	UTSW	12	51,816,065 (GRCm39)	missense	probably damaging	1.00
R6194:Hectd1	UTSW	12	51,795,228 (GRCm39)	missense	probably damaging	0.99
R6356:Hectd1	UTSW	12	51,791,402 (GRCm39)	missense	probably damaging	1.00
R6795:Hectd1	UTSW	12	51,841,270 (GRCm39)	missense	possibly damaging	0.94
R6909:Hectd1	UTSW	12	51,810,945 (GRCm39)	splice site	probably null
R6971:Hectd1	UTSW	12	51,795,526 (GRCm39)	nonsense	probably null
R7079:Hectd1	UTSW	12	51,834,638 (GRCm39)	missense	possibly damaging	0.96
R7104:Hectd1	UTSW	12	51,874,134 (GRCm39)	critical splice donor site	probably null
R7296:Hectd1	UTSW	12	51,832,635 (GRCm39)	missense	possibly damaging	0.73
R7346:Hectd1	UTSW	12	51,797,104 (GRCm39)	missense	probably benign
R7355:Hectd1	UTSW	12	51,838,081 (GRCm39)	missense	possibly damaging	0.72
R7468:Hectd1	UTSW	12	51,791,588 (GRCm39)	splice site	probably null
R7531:Hectd1	UTSW	12	51,853,150 (GRCm39)	missense	probably benign	0.33
R7532:Hectd1	UTSW	12	51,837,233 (GRCm39)	missense	probably damaging	0.98
R7755:Hectd1	UTSW	12	51,849,003 (GRCm39)	missense	possibly damaging	0.86
R7807:Hectd1	UTSW	12	51,792,171 (GRCm39)	missense	probably damaging	1.00
R7842:Hectd1	UTSW	12	51,819,343 (GRCm39)	missense	probably damaging	0.99
R7922:Hectd1	UTSW	12	51,836,978 (GRCm39)	nonsense	probably null
R8059:Hectd1	UTSW	12	51,837,161 (GRCm39)	missense	possibly damaging	0.53
R8085:Hectd1	UTSW	12	51,795,679 (GRCm39)	missense	probably damaging	0.97
R8145:Hectd1	UTSW	12	51,831,016 (GRCm39)	missense	possibly damaging	0.72
R8157:Hectd1	UTSW	12	51,838,073 (GRCm39)	missense	possibly damaging	0.53
R8405:Hectd1	UTSW	12	51,874,178 (GRCm39)	missense	probably benign	0.01
R8505:Hectd1	UTSW	12	51,797,145 (GRCm39)	missense	probably damaging	1.00
R8511:Hectd1	UTSW	12	51,834,654 (GRCm39)	missense	probably benign	0.01
R8697:Hectd1	UTSW	12	51,819,320 (GRCm39)	critical splice donor site	probably benign
R8725:Hectd1	UTSW	12	51,849,000 (GRCm39)	missense	possibly damaging	0.92
R8727:Hectd1	UTSW	12	51,849,000 (GRCm39)	missense	possibly damaging	0.92
R8911:Hectd1	UTSW	12	51,795,616 (GRCm39)	missense	probably damaging	0.99
R8983:Hectd1	UTSW	12	51,791,410 (GRCm39)	missense	probably damaging	0.97
R9037:Hectd1	UTSW	12	51,832,665 (GRCm39)	missense	possibly damaging	0.85
R9219:Hectd1	UTSW	12	51,800,612 (GRCm39)	missense	probably damaging	0.99
R9413:Hectd1	UTSW	12	51,792,880 (GRCm39)	nonsense	probably null
R9456:Hectd1	UTSW	12	51,832,584 (GRCm39)	missense	probably benign
R9513:Hectd1	UTSW	12	51,816,079 (GRCm39)	missense	possibly damaging	0.92
R9640:Hectd1	UTSW	12	51,795,197 (GRCm39)	nonsense	probably null
R9641:Hectd1	UTSW	12	51,816,047 (GRCm39)	missense	probably benign	0.00
R9713:Hectd1	UTSW	12	51,823,328 (GRCm39)	missense	probably benign	0.02

Predicted Primers

PCR Primer
(F):5'- CCTCACTGGGTCTTGCAAAG -3'
(R):5'- AGTCAAGTCTTGAAGCCAGGG -3'

Sequencing Primer
(F):5'- CTGGGTCTTGCAAAGAAACAAAC -3'
(R):5'- TCTTGAAGCCAGGGGAGTC -3'

Posted On

2019-06-26