Incidental Mutation 'R7176:Cd86'
ID 558644
Institutional Source Beutler Lab
Gene Symbol Cd86
Ensembl Gene ENSMUSG00000022901
Gene Name CD86 antigen
Synonyms MB7-2, Ly-58, Cd28l2, Ly58, B70, B7.2, B7-2
MMRRC Submission 045267-MU
Accession Numbers
Essential gene? Probably non essential (E-score: 0.084) question?
Stock # R7176 (G1)
Quality Score 217.468
Status Not validated
Chromosome 16
Chromosomal Location 36424231-36486443 bp(-) (GRCm39)
Type of Mutation frame shift
DNA Base Change (assembly) CA to CAA at 36426917 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change
Ref Sequence ENSEMBL: ENSMUSP00000087047 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000089620]
AlphaFold P42082
Predicted Effect probably null
Transcript: ENSMUST00000089620
SMART Domains Protein: ENSMUSP00000087047
Gene: ENSMUSG00000022901

DomainStartEndE-ValueType
signal peptide 1 23 N/A INTRINSIC
IGv 35 112 1.76e-8 SMART
low complexity region 194 205 N/A INTRINSIC
transmembrane domain 246 263 N/A INTRINSIC
Meta Mutation Damage Score 0.9756 question?
Coding Region Coverage
  • 1x: 100.0%
  • 3x: 100.0%
  • 10x: 99.7%
  • 20x: 98.9%
Validation Efficiency
MGI Phenotype FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. This protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of this protein with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in several transcript variants encoding different isoforms.[provided by RefSeq, May 2011]
PHENOTYPE: Homozygous null mice on an NOD background display a phenotype similar to human Guillain-Barre Syndrome, exhibiting severe peripheral nervous system inflammation, sciatic nerve demyelination, elevated auto-antibodies to myelin protein zero, hindlimb paralysis, and weak forelimb grip. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 68 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Abcc10 G T 17: 46,635,203 (GRCm39) H267N probably benign Het
Abtb2 A G 2: 103,539,720 (GRCm39) D695G probably benign Het
Adam21 C T 12: 81,607,022 (GRCm39) D247N possibly damaging Het
Adnp T C 2: 168,024,578 (GRCm39) N906D probably benign Het
Ankrd17 A T 5: 90,416,594 (GRCm39) H1079Q probably damaging Het
Aqp3 T A 4: 41,095,202 (GRCm39) N60Y probably damaging Het
Art4 T G 6: 136,834,166 (GRCm39) T26P probably benign Het
Arvcf T C 16: 18,218,477 (GRCm39) L553P probably damaging Het
Asxl1 T A 2: 153,243,908 (GRCm39) I1487N probably damaging Het
Capn3 A T 2: 120,334,973 (GRCm39) Y820F possibly damaging Het
Catip A G 1: 74,401,941 (GRCm39) T39A probably damaging Het
Ccdc168 A T 1: 44,099,506 (GRCm39) S531T probably benign Het
Ccdc18 T A 5: 108,315,972 (GRCm39) C481S probably benign Het
Ccnf A G 17: 24,468,376 (GRCm39) I7T possibly damaging Het
Cdkl4 A T 17: 80,851,221 (GRCm39) Y160* probably null Het
Celsr3 C T 9: 108,722,961 (GRCm39) P2783S probably benign Het
Cisd3 A T 11: 97,576,959 (GRCm39) D11V probably benign Het
Daxx A T 17: 34,132,292 (GRCm39) H512L unknown Het
Dip2b A G 15: 100,067,199 (GRCm39) H567R probably damaging Het
Dnah7c C A 1: 46,469,969 (GRCm39) A18E probably benign Het
Eif3d A T 15: 77,847,434 (GRCm39) V268D probably damaging Het
Eif4g3 T A 4: 137,898,497 (GRCm39) H1089Q probably damaging Het
Fmnl2 A G 2: 53,004,162 (GRCm39) M625V unknown Het
Foxn1 T C 11: 78,251,693 (GRCm39) R513G possibly damaging Het
Gemin5 C T 11: 58,056,828 (GRCm39) V134I probably benign Het
Gtf2h3 A G 5: 124,728,433 (GRCm39) R161G probably damaging Het
Ice1 A G 13: 70,772,525 (GRCm39) probably null Het
Il1rl1 T C 1: 40,485,766 (GRCm39) Y306H probably damaging Het
Kbtbd7 A T 14: 79,665,194 (GRCm39) E342V possibly damaging Het
Kyat3 A T 3: 142,443,600 (GRCm39) K404N possibly damaging Het
Lins1 T C 7: 66,363,553 (GRCm39) W483R probably benign Het
Lipo2 A G 19: 33,723,207 (GRCm39) I194T possibly damaging Het
Mcm8 G T 2: 132,661,992 (GRCm39) A137S probably benign Het
Mdm1 C A 10: 117,978,770 (GRCm39) Q12K probably damaging Het
Mrgprb5 C A 7: 47,818,059 (GRCm39) L225F possibly damaging Het
Myh11 T A 16: 14,033,690 (GRCm39) H1068L Het
Ngef A T 1: 87,408,417 (GRCm39) V550E possibly damaging Het
Nrg1 G A 8: 32,458,064 (GRCm39) Q84* probably null Het
Obp2b G T 2: 25,627,760 (GRCm39) V59L possibly damaging Het
Ocln C A 13: 100,651,590 (GRCm39) G327C probably damaging Het
Ocln A C 13: 100,651,591 (GRCm39) N326K probably benign Het
Or10ag55-ps1 C T 2: 87,115,378 (GRCm39) A248V probably damaging Het
Otogl T C 10: 107,614,772 (GRCm39) D1960G probably damaging Het
Pik3c2a T C 7: 115,987,331 (GRCm39) D530G possibly damaging Het
Plod1 A T 4: 147,997,744 (GRCm39) M655K probably benign Het
Ppp4r3b A G 11: 29,148,904 (GRCm39) N449D probably damaging Het
Ppp6r3 G T 19: 3,521,989 (GRCm39) T563K probably damaging Het
Prepl T A 17: 85,376,454 (GRCm39) L533F probably benign Het
Rbl1 G A 2: 157,030,245 (GRCm39) R421W probably damaging Het
Rrp15 G A 1: 186,453,730 (GRCm39) S239L probably benign Het
Runx2 G A 17: 45,125,079 (GRCm39) P80L probably damaging Het
Sbno1 A G 5: 124,530,944 (GRCm39) S815P probably benign Het
Scn7a G A 2: 66,506,632 (GRCm39) T1419I probably damaging Het
Setdb1 A G 3: 95,244,458 (GRCm39) probably null Het
Shpk A G 11: 73,113,814 (GRCm39) H409R probably benign Het
Slamf6 A C 1: 171,761,858 (GRCm39) N93T probably benign Het
Slc27a4 A G 2: 29,701,238 (GRCm39) N343S probably benign Het
Slc35g1 T A 19: 38,391,771 (GRCm39) V351E probably damaging Het
Smcr8 A T 11: 60,669,772 (GRCm39) I307F probably damaging Het
Spats2l T C 1: 57,977,077 (GRCm39) I305T possibly damaging Het
Speer4c1 T C 5: 15,916,536 (GRCm39) T144A probably benign Het
Tbl3 G A 17: 24,919,732 (GRCm39) T774I probably benign Het
Ush2a A G 1: 188,269,925 (GRCm39) H1724R probably benign Het
Vcan A G 13: 89,837,055 (GRCm39) S2830P probably benign Het
Vmn2r27 T A 6: 124,168,995 (GRCm39) I712F probably benign Het
Wdr59 T G 8: 112,219,388 (GRCm39) Q223P Het
Zfp28 T A 7: 6,386,456 (GRCm39) C22S possibly damaging Het
Zfp536 T G 7: 37,180,276 (GRCm39) probably null Het
Other mutations in Cd86
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01464:Cd86 APN 16 36,441,315 (GRCm39) missense probably benign 0.04
IGL01723:Cd86 APN 16 36,427,486 (GRCm39) missense probably benign
IGL01834:Cd86 APN 16 36,427,481 (GRCm39) missense probably benign 0.20
IGL02554:Cd86 APN 16 36,438,847 (GRCm39) missense probably benign 0.01
IGL02714:Cd86 APN 16 36,441,290 (GRCm39) missense possibly damaging 0.49
R0032:Cd86 UTSW 16 36,441,235 (GRCm39) missense probably damaging 0.96
R0032:Cd86 UTSW 16 36,441,235 (GRCm39) missense probably damaging 0.96
R0315:Cd86 UTSW 16 36,441,306 (GRCm39) missense possibly damaging 0.88
R0494:Cd86 UTSW 16 36,438,999 (GRCm39) splice site probably benign
R1345:Cd86 UTSW 16 36,438,686 (GRCm39) splice site probably null
R1459:Cd86 UTSW 16 36,449,350 (GRCm39) missense probably benign 0.09
R1616:Cd86 UTSW 16 36,449,338 (GRCm39) missense probably benign 0.00
R4436:Cd86 UTSW 16 36,441,194 (GRCm39) missense probably benign 0.04
R4593:Cd86 UTSW 16 36,426,918 (GRCm39) makesense probably null
R4612:Cd86 UTSW 16 36,435,692 (GRCm39) missense probably benign 0.00
R6058:Cd86 UTSW 16 36,449,377 (GRCm39) missense possibly damaging 0.91
R7140:Cd86 UTSW 16 36,441,263 (GRCm39) missense probably benign 0.09
R7174:Cd86 UTSW 16 36,426,917 (GRCm39) frame shift probably null
R7177:Cd86 UTSW 16 36,426,917 (GRCm39) frame shift probably null
R7181:Cd86 UTSW 16 36,426,917 (GRCm39) frame shift probably null
R7183:Cd86 UTSW 16 36,426,917 (GRCm39) frame shift probably null
R7232:Cd86 UTSW 16 36,426,917 (GRCm39) frame shift probably null
R7255:Cd86 UTSW 16 36,426,917 (GRCm39) frame shift probably null
R7256:Cd86 UTSW 16 36,426,917 (GRCm39) frame shift probably null
R7267:Cd86 UTSW 16 36,426,917 (GRCm39) frame shift probably null
R8826:Cd86 UTSW 16 36,435,650 (GRCm39) missense possibly damaging 0.45
R9595:Cd86 UTSW 16 36,441,275 (GRCm39) missense probably damaging 1.00
Predicted Primers PCR Primer
(F):5'- ACCTTGACTGACTCGGCATG -3'
(R):5'- ATATGAGGGTGGTTTTATGTCCAAC -3'

Sequencing Primer
(F):5'- GGACACTGCCACCTGTTTATGG -3'
(R):5'- GGTGGTTTTATGTCCAACATTAGAAG -3'
Posted On 2019-06-26