Mutagenetix > Incidental Mutation

Incidental Mutation 'R7209:Prmt9'

560938

Institutional Source

Beutler Lab

Gene Symbol

Prmt9

Ensembl Gene

ENSMUSG00000037134

Gene Name

protein arginine methyltransferase 9

Synonyms

Prmt10

MMRRC Submission

045338-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.226) question?

Stock #

R7209 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

78276026-78307967 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 78291627 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Valine to Alanine at position 333 (V333A)

Ref Sequence

ENSEMBL: ENSMUSP00000050181 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000056237] [ENSMUST00000118622] [ENSMUST00000210040]

AlphaFold

Q3U3W5

Predicted Effect

probably benign
Transcript: ENSMUST00000056237
AA Change: V333A

PolyPhen 2 Score 0.320 (Sensitivity: 0.90; Specificity: 0.89)

SMART Domains

Protein: ENSMUSP00000050181
Gene: ENSMUSG00000037134
AA Change: V333A

Domain	Start	End	E-Value	Type
low complexity region	5	21	N/A	INTRINSIC
Pfam:TPR_11	67	132	1.2e-7	PFAM
Pfam:TPR_2	102	134	7.9e-5	PFAM
Pfam:PrmA	168	257	2.5e-10	PFAM
internal_repeat_1	585	836	1.37e-10	PROSPERO

Predicted Effect

possibly damaging
Transcript: ENSMUST00000118622
AA Change: V333A

PolyPhen 2 Score 0.462 (Sensitivity: 0.89; Specificity: 0.90)

SMART Domains

Protein: ENSMUSP00000112692
Gene: ENSMUSG00000037134
AA Change: V333A

Domain	Start	End	E-Value	Type
low complexity region	5	21	N/A	INTRINSIC
Pfam:TPR_2	102	134	3e-5	PFAM
Pfam:PrmA	168	257	4.9e-10	PFAM
internal_repeat_1	585	836	1.05e-10	PROSPERO

Predicted Effect

probably benign
Transcript: ENSMUST00000210040

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 98.8%

Validation Efficiency

100% (67/67)

Allele List at MGI

Other mutations in this stock

Total: 69 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adam7	A	G	14: 68,767,268 (GRCm39)	Y61H	probably damaging	Het
Adgrb1	T	G	15: 74,441,797 (GRCm39)	V966G	possibly damaging	Het
Adh5	G	A	3: 138,148,909 (GRCm39)		probably benign	Het
Akr1b8	T	C	6: 34,333,207 (GRCm39)	V28A	probably damaging	Het
Apbb1	T	A	7: 105,215,292 (GRCm39)	I450F	probably damaging	Het
Arhgap23	C	T	11: 97,366,911 (GRCm39)	T1064I	probably damaging	Het
Arhgap23	G	C	11: 97,383,273 (GRCm39)		probably null	Het
Atg4b	A	G	1: 93,702,955 (GRCm39)	I128M	probably damaging	Het
Baat	A	T	4: 49,503,065 (GRCm39)	I19N	probably damaging	Het
Bspry	T	G	4: 62,404,852 (GRCm39)	I216S	possibly damaging	Het
Commd9	T	A	2: 101,725,483 (GRCm39)	S19T	possibly damaging	Het
Cr2	A	G	1: 194,851,032 (GRCm39)	C145R	probably damaging	Het
Cyp2c68	A	G	19: 39,677,649 (GRCm39)	L447P	probably damaging	Het
Depdc1b	T	C	13: 108,519,389 (GRCm39)	M333T	possibly damaging	Het
Dhx9	T	C	1: 153,340,369 (GRCm39)	T710A	possibly damaging	Het
Dnah5	T	C	15: 28,459,371 (GRCm39)	V4530A	possibly damaging	Het
Dohh	T	A	10: 81,221,874 (GRCm39)	H89Q	probably damaging	Het
Dop1b	T	A	16: 93,566,733 (GRCm39)	N1171K	probably benign	Het
Dscam	T	C	16: 96,451,544 (GRCm39)		probably null	Het
Entpd5	T	A	12: 84,443,702 (GRCm39)	S14C	probably benign	Het
Eqtn	G	A	4: 94,813,806 (GRCm39)	S125F	probably damaging	Het
Erp44	A	T	4: 48,211,704 (GRCm39)	D197E	probably benign	Het
Fgf18	A	T	11: 33,084,315 (GRCm39)	D46E	probably benign	Het
Foxa1	T	A	12: 57,590,077 (GRCm39)	M48L	probably benign	Het
Gabrg1	C	A	5: 70,911,513 (GRCm39)	C417F	probably damaging	Het
Glrp1	T	A	1: 88,431,004 (GRCm39)	Q122L	unknown	Het
Gm5622	A	G	14: 51,893,363 (GRCm39)	I97V	possibly damaging	Het
Gusb	A	G	5: 130,027,387 (GRCm39)	V306A	probably benign	Het
Hars1	A	G	18: 36,906,593 (GRCm39)	S126P	probably benign	Het
Herc1	A	G	9: 66,292,314 (GRCm39)	S356G	possibly damaging	Het
Hydin	C	A	8: 111,216,424 (GRCm39)	Y1336*	probably null	Het
Iftap	T	C	2: 101,396,727 (GRCm39)	*217W	probably null	Het
Kin	T	C	2: 10,096,564 (GRCm39)	Y138H	possibly damaging	Het
Lypd2	C	T	15: 74,604,266 (GRCm39)	V101M	probably benign	Het
Madcam1	A	T	10: 79,500,892 (GRCm39)	T70S	possibly damaging	Het
Man1a2	A	G	3: 100,554,395 (GRCm39)	C112R	unknown	Het
Mia2	T	A	12: 59,201,176 (GRCm39)	V198E	possibly damaging	Het
Mpdz	C	T	4: 81,225,114 (GRCm39)	V1438M	possibly damaging	Het
Mtif2	G	A	11: 29,479,996 (GRCm39)	V21M	probably benign	Het
Nbeal1	G	C	1: 60,276,310 (GRCm39)	V684L	probably benign	Het
Nln	G	A	13: 104,209,406 (GRCm39)	Q56*	probably null	Het
Nlrp4b	T	C	7: 10,444,297 (GRCm39)	V82A	probably benign	Het
Obscn	C	A	11: 58,975,933 (GRCm39)	E2065*	probably null	Het
Or10g1	A	G	14: 52,647,550 (GRCm39)	C260R	possibly damaging	Het
Pilrb2	C	T	5: 137,869,126 (GRCm39)		probably null	Het
Plekhh1	C	A	12: 79,097,150 (GRCm39)	D99E	probably benign	Het
Polr2b	T	A	5: 77,491,026 (GRCm39)	F956I	probably damaging	Het
Ppp3cc	A	G	14: 70,504,947 (GRCm39)	F20L	probably benign	Het
Psd4	T	C	2: 24,287,357 (GRCm39)	S430P	probably damaging	Het
Relch	C	A	1: 105,678,082 (GRCm39)	P1136T	probably damaging	Het
Rrp12	A	C	19: 41,861,388 (GRCm39)	V973G	possibly damaging	Het
Rxfp2	A	G	5: 149,976,563 (GRCm39)		probably null	Het
S100a6	G	A	3: 90,521,095 (GRCm39)	A8T	possibly damaging	Het
Sgsm2	C	A	11: 74,745,151 (GRCm39)	G717V	probably damaging	Het
Sipa1	A	G	19: 5,705,003 (GRCm39)	Y531H	probably damaging	Het
Slc45a1	A	C	4: 150,719,669 (GRCm39)		probably null	Het
Spopfm3	A	T	3: 94,106,012 (GRCm39)	Q110L	probably benign	Het
Srbd1	C	A	17: 86,308,948 (GRCm39)	L743F	probably damaging	Het
Taar2	A	T	10: 23,816,597 (GRCm39)	I46F	possibly damaging	Het
Tmem241	A	T	18: 12,237,229 (GRCm39)	V69D	probably damaging	Het
Tns1	T	C	1: 73,993,074 (GRCm39)	S535G	possibly damaging	Het
Trgc3	A	G	13: 19,445,334 (GRCm39)	N94S	probably benign	Het
Ttc34	C	A	4: 154,923,585 (GRCm39)	P98Q	probably damaging	Het
Ubr1	C	A	2: 120,693,246 (GRCm39)	R1720L	probably benign	Het
Ubr3	T	A	2: 69,846,478 (GRCm39)	D1597E	probably benign	Het
Unc79	T	C	12: 103,091,883 (GRCm39)	M1930T	probably benign	Het
Vmn2r100	A	T	17: 19,751,576 (GRCm39)	I603F	not run	Het
Vps26b	A	T	9: 26,921,288 (GRCm39)	S304T	probably benign	Het
Zfp952	A	T	17: 33,222,444 (GRCm39)	T308S	possibly damaging	Het

Other mutations in Prmt9

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01801:Prmt9	APN	8	78,289,069 (GRCm39)	missense	probably damaging	1.00
IGL02306:Prmt9	APN	8	78,287,447 (GRCm39)	missense	probably benign	0.06
IGL02971:Prmt9	APN	8	78,291,698 (GRCm39)	missense	probably benign	0.00
1mM(1):Prmt9	UTSW	8	78,282,393 (GRCm39)	missense	probably benign	0.01
R0004:Prmt9	UTSW	8	78,282,411 (GRCm39)	missense	possibly damaging	0.81
R0928:Prmt9	UTSW	8	78,307,805 (GRCm39)	missense	probably damaging	1.00
R1328:Prmt9	UTSW	8	78,299,283 (GRCm39)	missense	possibly damaging	0.86
R1777:Prmt9	UTSW	8	78,291,737 (GRCm39)	missense	probably benign	0.05
R1826:Prmt9	UTSW	8	78,282,303 (GRCm39)	nonsense	probably null
R1925:Prmt9	UTSW	8	78,303,968 (GRCm39)	missense	possibly damaging	0.52
R3855:Prmt9	UTSW	8	78,294,894 (GRCm39)	missense	probably benign	0.22
R3856:Prmt9	UTSW	8	78,294,894 (GRCm39)	missense	probably benign	0.22
R4089:Prmt9	UTSW	8	78,299,174 (GRCm39)	missense	probably benign	0.00
R4963:Prmt9	UTSW	8	78,282,358 (GRCm39)	missense	probably damaging	1.00
R5196:Prmt9	UTSW	8	78,291,626 (GRCm39)	missense	probably benign	0.30
R5413:Prmt9	UTSW	8	78,298,638 (GRCm39)	missense	possibly damaging	0.95
R5975:Prmt9	UTSW	8	78,287,647 (GRCm39)	intron	probably benign
R6271:Prmt9	UTSW	8	78,304,092 (GRCm39)	missense	probably damaging	0.96
R7023:Prmt9	UTSW	8	78,276,086 (GRCm39)	start gained	probably benign
R7107:Prmt9	UTSW	8	78,294,880 (GRCm39)	missense	possibly damaging	0.62
R7159:Prmt9	UTSW	8	78,282,393 (GRCm39)	missense	probably benign	0.01
R7770:Prmt9	UTSW	8	78,285,814 (GRCm39)	splice site	probably null
R7819:Prmt9	UTSW	8	78,294,973 (GRCm39)	missense	probably benign	0.11
R7959:Prmt9	UTSW	8	78,287,594 (GRCm39)	missense	probably damaging	1.00
R9106:Prmt9	UTSW	8	78,276,358 (GRCm39)	missense	probably benign	0.05
R9301:Prmt9	UTSW	8	78,282,374 (GRCm39)	nonsense	probably null
R9368:Prmt9	UTSW	8	78,285,663 (GRCm39)	missense	probably benign	0.00
R9665:Prmt9	UTSW	8	78,307,267 (GRCm39)	missense	probably benign	0.18
X0027:Prmt9	UTSW	8	78,287,512 (GRCm39)	missense	possibly damaging	0.68

Predicted Primers

PCR Primer
(F):5'- TTAAGACACCCGTGGCCAT -3'
(R):5'- GCTTCCCCGTCACACTGAAA -3'

Sequencing Primer
(F):5'- CCCGTGGCCATGAGGTTTTATTTC -3'
(R):5'- CCTGCTCTAAAGAGTGAGTTCCAG -3'

Posted On

2019-06-26