Mutagenetix > Incidental Mutation

Incidental Mutation 'R0594:Gtf2i'

56102

Institutional Source

Beutler Lab

Gene Symbol

Gtf2i

Ensembl Gene

ENSMUSG00000060261

Gene Name

general transcription factor II I

Synonyms

6030441I21Rik, TFII-I, BAP-135

MMRRC Submission

038784-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R0594 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

134266688-134343614 bp(-) (GRCm39)

Type of Mutation

splice site

DNA Base Change (assembly)

C to T at 134271027 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000133740 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000059042] [ENSMUST00000082057] [ENSMUST00000111261] [ENSMUST00000172715] [ENSMUST00000174155] [ENSMUST00000173888] [ENSMUST00000173341] [ENSMUST00000174354] [ENSMUST00000174513] [ENSMUST00000174772]

AlphaFold

Q9ESZ8

Predicted Effect

probably benign
Transcript: ENSMUST00000059042

SMART Domains

Protein: ENSMUSP00000049625
Gene: ENSMUSG00000060261

Domain	Start	End	E-Value	Type
low complexity region	82	94	N/A	INTRINSIC
Pfam:GTF2I	112	187	1.4e-34	PFAM
low complexity region	328	338	N/A	INTRINSIC
Pfam:GTF2I	361	436	3.4e-33	PFAM
Pfam:GTF2I	466	541	5e-34	PFAM
Pfam:GTF2I	571	646	3.3e-33	PFAM
Pfam:GTF2I	733	808	3.9e-33	PFAM
Pfam:GTF2I	868	943	9.4e-30	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000082057

SMART Domains

Protein: ENSMUSP00000080714
Gene: ENSMUSG00000060261

Domain	Start	End	E-Value	Type
low complexity region	82	94	N/A	INTRINSIC
Pfam:GTF2I	112	187	1.3e-34	PFAM
low complexity region	307	317	N/A	INTRINSIC
Pfam:GTF2I	340	415	3.3e-33	PFAM
Pfam:GTF2I	445	520	4.9e-34	PFAM
Pfam:GTF2I	550	625	3.2e-33	PFAM
Pfam:GTF2I	712	787	3.8e-33	PFAM
Pfam:GTF2I	847	922	9.1e-30	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000111261

SMART Domains

Protein: ENSMUSP00000106892
Gene: ENSMUSG00000060261

Domain	Start	End	E-Value	Type
low complexity region	82	94	N/A	INTRINSIC
Pfam:GTF2I	112	187	1.3e-34	PFAM
low complexity region	309	319	N/A	INTRINSIC
Pfam:GTF2I	342	417	3.3e-33	PFAM
Pfam:GTF2I	447	522	4.9e-34	PFAM
Pfam:GTF2I	552	627	3.2e-33	PFAM
Pfam:GTF2I	714	789	3.8e-33	PFAM
Pfam:GTF2I	849	924	9.1e-30	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000172553

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000172600

Predicted Effect

probably benign
Transcript: ENSMUST00000172715

SMART Domains

Protein: ENSMUSP00000134035
Gene: ENSMUSG00000060261

Domain	Start	End	E-Value	Type
low complexity region	82	94	N/A	INTRINSIC
Pfam:GTF2I	112	187	9.7e-35	PFAM
low complexity region	243	253	N/A	INTRINSIC
Pfam:GTF2I	276	351	2.4e-33	PFAM
Pfam:GTF2I	381	456	3.6e-34	PFAM
Pfam:GTF2I	486	561	2.4e-33	PFAM
Pfam:GTF2I	648	723	2.8e-33	PFAM
Pfam:GTF2I	783	858	6.7e-30	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000172904

Predicted Effect

probably benign
Transcript: ENSMUST00000174155

SMART Domains

Protein: ENSMUSP00000133566
Gene: ENSMUSG00000060261

Domain	Start	End	E-Value	Type
low complexity region	82	94	N/A	INTRINSIC
Pfam:GTF2I	112	186	1.6e-34	PFAM
low complexity region	328	338	N/A	INTRINSIC
Pfam:GTF2I	361	435	3e-33	PFAM
Pfam:GTF2I	466	540	9.1e-34	PFAM
Pfam:GTF2I	571	645	1.8e-32	PFAM
Pfam:GTF2I	733	807	2.2e-33	PFAM
Pfam:GTF2I	868	942	6e-27	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000173888

SMART Domains

Protein: ENSMUSP00000133969
Gene: ENSMUSG00000060261

Domain	Start	End	E-Value	Type
low complexity region	82	94	N/A	INTRINSIC
Pfam:GTF2I	112	187	1.3e-34	PFAM
low complexity region	245	258	N/A	INTRINSIC
low complexity region	269	279	N/A	INTRINSIC
Pfam:GTF2I	302	377	3.2e-33	PFAM
Pfam:GTF2I	407	482	4.6e-34	PFAM
Pfam:GTF2I	512	587	3.1e-33	PFAM
Pfam:GTF2I	674	749	3.6e-33	PFAM
Pfam:GTF2I	809	884	8.7e-30	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000174003

Predicted Effect

probably benign
Transcript: ENSMUST00000173341

SMART Domains

Protein: ENSMUSP00000133444
Gene: ENSMUSG00000060261

Domain	Start	End	E-Value	Type
low complexity region	82	94	N/A	INTRINSIC
Pfam:GTF2I	112	187	1e-34	PFAM
low complexity region	288	298	N/A	INTRINSIC
Pfam:GTF2I	321	396	2.6e-33	PFAM
Pfam:GTF2I	426	501	3.8e-34	PFAM
Pfam:GTF2I	531	606	2.5e-33	PFAM
Pfam:GTF2I	693	768	3e-33	PFAM
Pfam:GTF2I	832	907	7.1e-30	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000174354

SMART Domains

Protein: ENSMUSP00000134440
Gene: ENSMUSG00000060261

Domain	Start	End	E-Value	Type
low complexity region	82	94	N/A	INTRINSIC
Pfam:GTF2I	112	187	1.3e-34	PFAM
low complexity region	309	319	N/A	INTRINSIC
Pfam:GTF2I	342	417	3.3e-33	PFAM
Pfam:GTF2I	447	522	4.9e-34	PFAM
Pfam:GTF2I	552	627	3.2e-33	PFAM
Pfam:GTF2I	714	789	3.8e-33	PFAM
Pfam:GTF2I	849	924	9.1e-30	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000173485

SMART Domains

Protein: ENSMUSP00000133526
Gene: ENSMUSG00000060261

Domain	Start	End	E-Value	Type
Pfam:GTF2I	35	109	2.4e-28	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000174513

SMART Domains

Protein: ENSMUSP00000133489
Gene: ENSMUSG00000060261

Domain	Start	End	E-Value	Type
low complexity region	82	94	N/A	INTRINSIC
Pfam:GTF2I	112	187	1.3e-34	PFAM
low complexity region	288	298	N/A	INTRINSIC
Pfam:GTF2I	321	396	3.2e-33	PFAM
Pfam:GTF2I	426	501	4.8e-34	PFAM
Pfam:GTF2I	531	606	3.2e-33	PFAM
Pfam:GTF2I	693	768	3.7e-33	PFAM
Pfam:GTF2I	828	903	8.9e-30	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000174772

SMART Domains

Protein: ENSMUSP00000133740
Gene: ENSMUSG00000060261

Domain	Start	End	E-Value	Type
low complexity region	82	94	N/A	INTRINSIC
Pfam:GTF2I	112	187	1.3e-34	PFAM
low complexity region	307	317	N/A	INTRINSIC
Pfam:GTF2I	340	415	3.3e-33	PFAM
Pfam:GTF2I	445	520	4.9e-34	PFAM
Pfam:GTF2I	550	625	3.2e-33	PFAM
Pfam:GTF2I	712	787	3.8e-33	PFAM
Pfam:GTF2I	847	922	9.1e-30	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000173509

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 99.4%
3x: 98.9%
10x: 97.4%
20x: 94.6%

Validation Efficiency

99% (119/120)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a phosphoprotein containing six characteristic repeat motifs. The encoded protein binds to the initiator element (Inr) and E-box element in promoters and functions as a regulator of transcription. This locus, along with several other neighboring genes, is deleted in Williams-Beuren syndrome. There are many closely related genes and pseudogenes for this gene on chromosome 7. This gene also has pseudogenes on chromosomes 9, 13, and 21. Alternatively spliced transcript variants encoding multiple isoforms have been observed. [provided by RefSeq, Jul 2013]
PHENOTYPE: Homozygotes for null allele is embryonic lethal, and show brain hemorrhage and neural tube defects. Although most heterozygote are normal and fertile, at low frequency, growth retardation and small head are also reported. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 116 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abcc1	T	C	16: 14,207,744 (GRCm39)	V41A	probably benign	Het
Acad11	A	G	9: 103,972,762 (GRCm39)	Q367R	probably benign	Het
Ackr4	A	G	9: 103,976,203 (GRCm39)	V248A	possibly damaging	Het
Adamts14	T	C	10: 61,038,666 (GRCm39)	E945G	probably damaging	Het
Ano2	A	G	6: 125,959,728 (GRCm39)	M663V	probably damaging	Het
Apc2	T	A	10: 80,142,090 (GRCm39)	C336*	probably null	Het
Arhgap17	A	G	7: 122,893,741 (GRCm39)	S560P	probably benign	Het
Arl5a	T	C	2: 52,295,026 (GRCm39)	D128G	probably damaging	Het
Atp6v0a2	C	A	5: 124,795,046 (GRCm39)	R678S	probably benign	Het
B4galnt2	C	A	11: 95,782,735 (GRCm39)	A26S	probably benign	Het
C1qtnf1	A	T	11: 118,337,454 (GRCm39)	T95S	possibly damaging	Het
Ccdc188	T	A	16: 18,036,784 (GRCm39)	F241L	probably benign	Het
Cdh19	A	T	1: 110,853,597 (GRCm39)	D281E	probably benign	Het
Cdk5rap2	T	C	4: 70,273,050 (GRCm39)	E241G	probably damaging	Het
Cherp	A	T	8: 73,216,246 (GRCm39)		probably null	Het
Cpne9	T	A	6: 113,267,361 (GRCm39)		probably benign	Het
Cthrc1	A	T	15: 38,940,537 (GRCm39)	R47W	possibly damaging	Het
Dcaf13	A	G	15: 38,986,663 (GRCm39)	E145G	probably benign	Het
Dcaf4	T	A	12: 83,584,817 (GRCm39)		probably null	Het
Dgka	A	C	10: 128,568,979 (GRCm39)		probably benign	Het
Dhrs13	T	A	11: 77,925,351 (GRCm39)	F157L	probably damaging	Het
Dnajb5	A	T	4: 42,956,577 (GRCm39)	Y88F	probably damaging	Het
Dpp8	A	G	9: 64,944,280 (GRCm39)	T16A	probably damaging	Het
Dscc1	A	T	15: 54,952,448 (GRCm39)	I91K	possibly damaging	Het
Efemp2	T	A	19: 5,525,091 (GRCm39)		probably benign	Het
Elf2	T	C	3: 51,163,874 (GRCm39)	T504A	possibly damaging	Het
Elk3	G	A	10: 93,101,022 (GRCm39)	S243F	probably damaging	Het
Ell2	A	G	13: 75,898,112 (GRCm39)	D93G	probably damaging	Het
Eln	G	T	5: 134,741,252 (GRCm39)		probably benign	Het
Eme1	C	T	11: 94,541,256 (GRCm39)	D189N	possibly damaging	Het
Epb41l2	A	G	10: 25,319,668 (GRCm39)	E167G	possibly damaging	Het
Exoc5	A	T	14: 49,273,544 (GRCm39)		probably benign	Het
Fam170b	A	G	14: 32,558,271 (GRCm39)	K369E	unknown	Het
Fam187b	T	A	7: 30,676,579 (GRCm39)	C29*	probably null	Het
Fam20c	T	C	5: 138,752,392 (GRCm39)	S260P	possibly damaging	Het
Fam216b	G	A	14: 78,324,114 (GRCm39)	A21V	possibly damaging	Het
Fam98a	A	T	17: 75,845,482 (GRCm39)	Y421*	probably null	Het
Farp2	T	C	1: 93,504,222 (GRCm39)	V333A	probably damaging	Het
Fcgr1	T	C	3: 96,199,628 (GRCm39)	Y93C	probably damaging	Het
Fgd2	A	T	17: 29,584,526 (GRCm39)	I157F	probably damaging	Het
Frmd4b	T	A	6: 97,302,387 (GRCm39)		probably benign	Het
Fut9	T	C	4: 25,620,526 (GRCm39)	D96G	possibly damaging	Het
Glt8d1	G	A	14: 30,732,367 (GRCm39)		probably null	Het
Gm7579	T	A	7: 141,766,121 (GRCm39)	C176S	unknown	Het
Gmpr2	A	G	14: 55,915,445 (GRCm39)	E272G	probably damaging	Het
Grin2b	T	C	6: 135,710,927 (GRCm39)	H873R	probably damaging	Het
Htr3b	A	T	9: 48,858,931 (GRCm39)	V69E	probably benign	Het
Icam5	A	G	9: 20,946,894 (GRCm39)	N474S	probably benign	Het
Itgal	T	A	7: 126,913,232 (GRCm39)	S610T	probably damaging	Het
Jag1	T	A	2: 136,929,000 (GRCm39)	I819L	probably damaging	Het
Kif9	A	T	9: 110,340,408 (GRCm39)	E467V	probably benign	Het
Krit1	T	C	5: 3,873,694 (GRCm39)	L491P	possibly damaging	Het
Lipo2	T	G	19: 33,724,302 (GRCm39)	I155L	possibly damaging	Het
Lmbr1	A	G	5: 29,497,207 (GRCm39)	F65L	possibly damaging	Het
Lsp1	G	A	7: 142,042,687 (GRCm39)		probably benign	Het
Mgat5	T	A	1: 127,339,985 (GRCm39)	D455E	probably damaging	Het
Mical2	A	T	7: 111,917,657 (GRCm39)	Y338F	probably damaging	Het
Mre11a	T	G	9: 14,726,505 (GRCm39)	S396A	probably benign	Het
Mrtfa	G	A	15: 80,901,375 (GRCm39)	T372I	probably damaging	Het
Mtarc2	T	C	1: 184,573,536 (GRCm39)	N121D	probably benign	Het
Myo3a	C	T	2: 22,436,370 (GRCm39)		probably benign	Het
Naca	T	C	10: 127,876,224 (GRCm39)		probably benign	Het
Nav1	A	T	1: 135,395,381 (GRCm39)	I996K	possibly damaging	Het
Ncbp1	A	G	4: 46,170,551 (GRCm39)	N742S	probably benign	Het
Ndufaf3	G	A	9: 108,444,122 (GRCm39)	A2V	probably benign	Het
Niban3	C	A	8: 72,051,779 (GRCm39)	A38E	probably benign	Het
Ntn5	G	T	7: 45,336,105 (GRCm39)	A47S	probably damaging	Het
Or10ag57	T	A	2: 87,218,298 (GRCm39)	I83N	probably damaging	Het
Or1e25	G	A	11: 73,494,218 (GRCm39)	E271K	probably benign	Het
Or1e32	T	C	11: 73,705,443 (GRCm39)	H155R	probably benign	Het
Or2a7	T	A	6: 43,151,541 (GRCm39)	V207E	possibly damaging	Het
Or6c207	T	C	10: 129,105,021 (GRCm39)	Y57C	possibly damaging	Het
Or9i1	G	T	19: 13,839,643 (GRCm39)	C162F	probably benign	Het
Otud7a	T	A	7: 63,377,220 (GRCm39)	L203*	probably null	Het
Pakap	A	G	4: 57,856,752 (GRCm39)	T694A	probably benign	Het
Pcdhb13	A	G	18: 37,576,984 (GRCm39)	Y454C	probably damaging	Het
Pdzph1	C	T	17: 59,261,474 (GRCm39)	V853M	possibly damaging	Het
Plec	A	G	15: 76,056,453 (GRCm39)	S4517P	probably damaging	Het
Pm20d2	C	T	4: 33,181,746 (GRCm39)	E286K	probably damaging	Het
Polr2i	T	A	7: 29,932,170 (GRCm39)		probably null	Het
Ppp1r12b	A	G	1: 134,704,217 (GRCm39)	L879P	probably damaging	Het
Prf1	C	A	10: 61,139,501 (GRCm39)	Y486*	probably null	Het
Qsox2	T	G	2: 26,104,056 (GRCm39)	T325P	probably damaging	Het
Rab1b	G	T	19: 5,150,684 (GRCm39)		probably benign	Het
Rbm19	T	C	5: 120,266,381 (GRCm39)		probably null	Het
Rhobtb2	A	G	14: 70,031,397 (GRCm39)	V576A	probably benign	Het
Rnps1	G	A	17: 24,643,411 (GRCm39)	V215M	probably damaging	Het
Rps11	A	G	7: 44,773,706 (GRCm39)		probably benign	Het
Serpinb3d	C	T	1: 107,007,077 (GRCm39)	M210I	probably damaging	Het
Sgsm1	T	C	5: 113,458,428 (GRCm39)	T17A	probably benign	Het
Slc6a3	A	G	13: 73,686,761 (GRCm39)	T43A	probably damaging	Het
Sox4	C	G	13: 29,136,887 (GRCm39)	A40P	probably damaging	Het
Spry2	A	T	14: 106,130,744 (GRCm39)	D147E	possibly damaging	Het
Stpg1	A	G	4: 135,246,742 (GRCm39)	N157D	possibly damaging	Het
Sumf1	T	C	6: 108,150,375 (GRCm39)	D152G	probably benign	Het
Tbr1	T	C	2: 61,641,964 (GRCm39)	S410P	possibly damaging	Het
Tdrd6	A	G	17: 43,940,274 (GRCm39)	V258A	probably damaging	Het
Tirap	C	T	9: 35,100,057 (GRCm39)	G209D	probably damaging	Het
Tnfrsf8	A	T	4: 145,023,431 (GRCm39)	V134D	probably damaging	Het
Tnr	A	G	1: 159,677,905 (GRCm39)	T97A	probably benign	Het
Tspan32	T	A	7: 142,569,347 (GRCm39)	F135L	probably damaging	Het
Ttn	T	C	2: 76,619,400 (GRCm39)	K16021E	probably damaging	Het
Tusc3	T	A	8: 39,564,122 (GRCm39)	I251N	probably damaging	Het
Usp38	A	T	8: 81,731,995 (GRCm39)	I305N	probably damaging	Het
Usp4	T	A	9: 108,248,080 (GRCm39)		probably null	Het
Usp5	A	T	6: 124,794,387 (GRCm39)	D764E	probably damaging	Het
Vangl2	A	T	1: 171,832,224 (GRCm39)	V544E	probably damaging	Het
Vldlr	G	A	19: 27,212,219 (GRCm39)	V78M	probably damaging	Het
Vmn1r29	T	C	6: 58,284,757 (GRCm39)	V159A	probably benign	Het
Vmn2r16	T	A	5: 109,511,762 (GRCm39)	F656L	probably damaging	Het
Wdfy3	T	A	5: 102,054,051 (GRCm39)	I1590F	possibly damaging	Het
Xpo1	T	A	11: 23,230,402 (GRCm39)	V263E	probably damaging	Het
Zbtb38	A	G	9: 96,568,007 (GRCm39)	S1026P	probably damaging	Het
Zfp407	A	T	18: 84,580,692 (GRCm39)	D140E	possibly damaging	Het
Zfp637	T	A	6: 117,822,647 (GRCm39)	Y258*	probably null	Het
Zfp951	T	A	5: 104,962,438 (GRCm39)	Q376L	possibly damaging	Het

Other mutations in Gtf2i

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00155:Gtf2i	APN	5	134,271,602 (GRCm39)	missense	probably damaging	1.00
IGL01565:Gtf2i	APN	5	134,284,767 (GRCm39)	missense	probably damaging	0.97
IGL01743:Gtf2i	APN	5	134,315,747 (GRCm39)	missense	probably damaging	1.00
IGL01809:Gtf2i	APN	5	134,278,804 (GRCm39)	missense	probably damaging	1.00
IGL02553:Gtf2i	APN	5	134,274,015 (GRCm39)	missense	probably damaging	1.00
IGL02814:Gtf2i	APN	5	134,315,558 (GRCm39)	missense	probably damaging	1.00
IGL02869:Gtf2i	APN	5	134,308,281 (GRCm39)	splice site	probably benign
IGL03018:Gtf2i	APN	5	134,318,189 (GRCm39)	missense	possibly damaging	0.79
IGL03051:Gtf2i	APN	5	134,271,768 (GRCm39)	nonsense	probably null
P0041:Gtf2i	UTSW	5	134,273,742 (GRCm39)	splice site	probably benign
R0330:Gtf2i	UTSW	5	134,280,740 (GRCm39)	missense	probably damaging	0.98
R0515:Gtf2i	UTSW	5	134,271,773 (GRCm39)	missense	probably damaging	1.00
R0529:Gtf2i	UTSW	5	134,290,723 (GRCm39)	nonsense	probably null
R0650:Gtf2i	UTSW	5	134,290,691 (GRCm39)	splice site	probably benign
R1055:Gtf2i	UTSW	5	134,292,478 (GRCm39)	missense	probably damaging	1.00
R1079:Gtf2i	UTSW	5	134,271,748 (GRCm39)	splice site	probably benign
R1916:Gtf2i	UTSW	5	134,275,702 (GRCm39)	missense	probably damaging	1.00
R2969:Gtf2i	UTSW	5	134,280,746 (GRCm39)	missense	probably damaging	0.98
R3013:Gtf2i	UTSW	5	134,324,358 (GRCm39)	splice site	probably benign
R4392:Gtf2i	UTSW	5	134,289,483 (GRCm39)	missense	probably damaging	0.96
R4421:Gtf2i	UTSW	5	134,283,891 (GRCm39)	missense	possibly damaging	0.86
R4635:Gtf2i	UTSW	5	134,274,028 (GRCm39)	missense	probably damaging	1.00
R4763:Gtf2i	UTSW	5	134,284,818 (GRCm39)	missense	probably damaging	1.00
R4770:Gtf2i	UTSW	5	134,272,414 (GRCm39)	missense	possibly damaging	0.53
R5063:Gtf2i	UTSW	5	134,289,425 (GRCm39)	missense	probably damaging	1.00
R5195:Gtf2i	UTSW	5	134,273,686 (GRCm39)	nonsense	probably null
R5829:Gtf2i	UTSW	5	134,292,547 (GRCm39)	missense	probably damaging	1.00
R6005:Gtf2i	UTSW	5	134,284,812 (GRCm39)	nonsense	probably null
R6119:Gtf2i	UTSW	5	134,315,911 (GRCm39)	splice site	probably null
R6576:Gtf2i	UTSW	5	134,292,556 (GRCm39)	missense	probably damaging	1.00
R6936:Gtf2i	UTSW	5	134,271,639 (GRCm39)	missense	probably damaging	1.00
R7070:Gtf2i	UTSW	5	134,311,657 (GRCm39)	missense	probably damaging	1.00
R7071:Gtf2i	UTSW	5	134,292,475 (GRCm39)	missense	probably damaging	1.00
R7142:Gtf2i	UTSW	5	134,273,705 (GRCm39)	missense	possibly damaging	0.95
R7366:Gtf2i	UTSW	5	134,294,603 (GRCm39)	missense	probably damaging	1.00
R7694:Gtf2i	UTSW	5	134,311,659 (GRCm39)	missense	probably damaging	1.00
R7879:Gtf2i	UTSW	5	134,295,471 (GRCm39)	missense	possibly damaging	0.77
R8039:Gtf2i	UTSW	5	134,284,688 (GRCm39)	missense	possibly damaging	0.95
R8041:Gtf2i	UTSW	5	134,322,599 (GRCm39)	critical splice donor site	probably null
R8154:Gtf2i	UTSW	5	134,280,721 (GRCm39)	missense	probably benign	0.07
R8365:Gtf2i	UTSW	5	134,303,434 (GRCm39)	missense	probably benign	0.22
R8706:Gtf2i	UTSW	5	134,278,733 (GRCm39)	missense	probably damaging	0.97
R8738:Gtf2i	UTSW	5	134,324,374 (GRCm39)	missense	probably damaging	1.00
R8827:Gtf2i	UTSW	5	134,269,096 (GRCm39)	critical splice donor site	probably null
R8901:Gtf2i	UTSW	5	134,324,389 (GRCm39)	missense	probably damaging	1.00
R8902:Gtf2i	UTSW	5	134,278,720 (GRCm39)	missense	probably benign	0.00
R9020:Gtf2i	UTSW	5	134,275,415 (GRCm39)	missense	probably benign	0.03
R9092:Gtf2i	UTSW	5	134,318,241 (GRCm39)	makesense	probably null
R9169:Gtf2i	UTSW	5	134,271,534 (GRCm39)	missense	probably damaging	1.00
R9240:Gtf2i	UTSW	5	134,292,619 (GRCm39)	missense	probably benign	0.34
R9333:Gtf2i	UTSW	5	134,271,840 (GRCm39)	missense	probably benign	0.01
R9346:Gtf2i	UTSW	5	134,315,781 (GRCm39)	missense	probably benign
R9346:Gtf2i	UTSW	5	134,273,663 (GRCm39)	missense	probably damaging	1.00
R9570:Gtf2i	UTSW	5	134,294,627 (GRCm39)	missense	probably damaging	1.00
R9648:Gtf2i	UTSW	5	134,284,770 (GRCm39)	missense	probably damaging	1.00
R9702:Gtf2i	UTSW	5	134,275,415 (GRCm39)	missense	probably benign	0.03
X0022:Gtf2i	UTSW	5	134,292,470 (GRCm39)	missense	probably damaging	1.00
Z1176:Gtf2i	UTSW	5	134,292,499 (GRCm39)	missense	probably null	1.00

Predicted Primers

PCR Primer
(F):5'- ATTTCACAAGCAGGCAGGGTGC -3'
(R):5'- TCACGGCTAGGTCATATCCCAGAG -3'

Sequencing Primer
(F):5'- GTGCTGAGAACGGGTGAC -3'
(R):5'- cccccacaccccaaaaac -3'

Posted On

2013-07-11