Mutagenetix > Incidental Mutation

Incidental Mutation 'R7232:Or10a2'

562555

Institutional Source

Beutler Lab

Gene Symbol

Or10a2

Ensembl Gene

ENSMUSG00000049674

Gene Name

olfactory receptor family 10 subfamily A member 2

Synonyms

GA_x6K02T2PBJ9-9453401-9454354, Olfr714, P4, MOR263-2

MMRRC Submission

045303-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.069) question?

Stock #

R7232 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

106673037-106673990 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 106673062 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Lysine at position 9 (I9K)

Ref Sequence

ENSEMBL: ENSMUSP00000151106 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000054629] [ENSMUST00000214429]

AlphaFold

Q7TRN0

Predicted Effect

probably benign
Transcript: ENSMUST00000054629
AA Change: I9K

PolyPhen 2 Score 0.028 (Sensitivity: 0.95; Specificity: 0.81)

SMART Domains

Protein: ENSMUSP00000095742
Gene: ENSMUSG00000049674
AA Change: I9K

Domain	Start	End	E-Value	Type
Pfam:7tm_4	32	309	8.5e-56	PFAM
Pfam:7tm_1	42	291	3.5e-24	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000214429
AA Change: I9K

PolyPhen 2 Score 0.028 (Sensitivity: 0.95; Specificity: 0.81)

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.6%
20x: 98.7%

Validation Efficiency

99% (80/81)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]
PHENOTYPE: Mice homozygous for a reporter allele exhibit abnormal olfactory sensory neuron projections. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 79 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930433I11Rik	G	A	7: 40,642,603 (GRCm39)	G91S	probably damaging	Het
Adam21	T	G	12: 81,607,330 (GRCm39)	N144T	probably damaging	Het
Adgrg7	T	C	16: 56,597,515 (GRCm39)		probably null	Het
Angpt4	T	C	2: 151,771,460 (GRCm39)	S259P	possibly damaging	Het
Aqr	A	C	2: 113,936,363 (GRCm39)	L1320R	probably damaging	Het
Arhgef10	G	A	8: 14,990,323 (GRCm39)	G266D	probably benign	Het
Bop1	A	G	15: 76,337,546 (GRCm39)	V693A	probably damaging	Het
Cd86	CA	CAA	16: 36,426,917 (GRCm39)		probably null	Het
Cdc5l	A	G	17: 45,738,863 (GRCm39)		probably null	Het
Cfap46	C	A	7: 139,197,493 (GRCm39)	R2126L	unknown	Het
Chek2	T	C	5: 111,008,781 (GRCm39)	V304A	probably damaging	Het
Cntnap4	T	A	8: 113,391,731 (GRCm39)		probably null	Het
Ctps1	C	A	4: 120,405,321 (GRCm39)	G374C	probably damaging	Het
Defa27	A	T	8: 21,805,625 (GRCm39)	I22F	probably damaging	Het
Dnah14	T	C	1: 181,584,928 (GRCm39)	S3220P	probably damaging	Het
Dop1b	T	G	16: 93,557,373 (GRCm39)		probably null	Het
Dpysl5	G	A	5: 30,949,642 (GRCm39)	V471I	probably benign	Het
Duoxa1	T	A	2: 122,135,728 (GRCm39)	I124F	probably damaging	Het
Eif4enif1	A	G	11: 3,165,678 (GRCm39)	E85G	possibly damaging	Het
Eogt	T	A	6: 97,096,944 (GRCm39)	I355F	probably damaging	Het
Epha7	T	A	4: 28,951,279 (GRCm39)	V800E	probably damaging	Het
Evi5l	A	G	8: 4,255,906 (GRCm39)	Q633R	possibly damaging	Het
Fam170a	T	C	18: 50,414,728 (GRCm39)	Y125H	probably damaging	Het
Fbxo15	T	C	18: 84,980,747 (GRCm39)	Y241H	probably damaging	Het
Gbe1	T	C	16: 70,233,828 (GRCm39)	I235T	possibly damaging	Het
Gfra3	C	T	18: 34,844,234 (GRCm39)	R102Q	probably damaging	Het
Gjd4	C	T	18: 9,280,380 (GRCm39)	G233S	probably damaging	Het
Gm29106	C	T	1: 118,127,291 (GRCm39)	P328S	probably damaging	Het
Hgfac	G	T	5: 35,204,258 (GRCm39)	R507L	probably damaging	Het
Jakmip3	A	G	7: 138,609,355 (GRCm39)	K153R	probably benign	Het
Kcnc1	T	A	7: 46,077,383 (GRCm39)	V395E	probably damaging	Het
Krt6b	T	C	15: 101,586,577 (GRCm39)	D304G	probably damaging	Het
Ldha	T	C	7: 46,500,323 (GRCm39)	Y174H	probably benign	Het
Lgi4	G	A	7: 30,766,776 (GRCm39)	V268M	possibly damaging	Het
Lpar3	T	G	3: 145,947,061 (GRCm39)		probably null	Het
Lrp3	T	C	7: 34,905,477 (GRCm39)	D103G	probably damaging	Het
Marchf5	G	A	19: 37,194,713 (GRCm39)		probably null	Het
Muc5b	A	T	7: 141,419,866 (GRCm39)	H4115L	possibly damaging	Het
Myh13	G	A	11: 67,239,672 (GRCm39)	D741N	probably damaging	Het
Naglu	A	T	11: 100,967,252 (GRCm39)	I401F	probably damaging	Het
Ncam2	T	A	16: 81,309,759 (GRCm39)	N416K	probably damaging	Het
Ncan	A	G	8: 70,564,738 (GRCm39)	L292S	probably damaging	Het
Nkiras1	T	C	14: 18,276,732 (GRCm38)	V7A	probably damaging	Het
Nlrp4c	T	A	7: 6,068,708 (GRCm39)	L203*	probably null	Het
Nscme3l	A	T	19: 5,553,659 (GRCm39)	S41T	possibly damaging	Het
Onecut2	T	A	18: 64,474,633 (GRCm39)	W395R	probably damaging	Het
Pak6	T	A	2: 118,524,003 (GRCm39)	V386E	probably damaging	Het
Pias2	A	G	18: 77,220,931 (GRCm39)	S396G	probably benign	Het
Plxna2	T	A	1: 194,394,568 (GRCm39)	L483H	probably damaging	Het
Prss36	G	T	7: 127,534,763 (GRCm39)	R484S	probably benign	Het
Ptpn21	A	T	12: 98,654,996 (GRCm39)	V657E	probably benign	Het
Rassf2	T	C	2: 131,838,332 (GRCm39)	E318G	probably damaging	Het
Rp1	A	C	1: 4,298,824 (GRCm39)	S623A	unknown	Het
Runx2	G	A	17: 45,125,079 (GRCm39)	P80L	probably damaging	Het
Scn11a	T	A	9: 119,588,982 (GRCm39)	E1308V	probably damaging	Het
Serpina3c	A	T	12: 104,115,771 (GRCm39)	S258T	possibly damaging	Het
Slit3	A	G	11: 35,501,516 (GRCm39)	T417A	possibly damaging	Het
Sostdc1	C	A	12: 36,367,310 (GRCm39)	A162E	possibly damaging	Het
Sox13	A	T	1: 133,312,129 (GRCm39)		probably null	Het
Sox8	G	T	17: 25,786,514 (GRCm39)	S396R	probably benign	Het
Sult2a3	A	T	7: 13,816,685 (GRCm39)	F164L	possibly damaging	Het
Susd2	T	C	10: 75,475,685 (GRCm39)	Y438C	probably damaging	Het
Tas2r106	T	G	6: 131,655,810 (GRCm39)	T14P	probably damaging	Het
Tcf25	T	A	8: 124,127,800 (GRCm39)		probably null	Het
Tenm3	G	A	8: 48,688,970 (GRCm39)	R2206W	probably damaging	Het
Tep1	A	G	14: 51,081,789 (GRCm39)	L471P	unknown	Het
Ticrr	A	T	7: 79,343,490 (GRCm39)	K1118N	probably damaging	Het
Tlr5	G	A	1: 182,801,064 (GRCm39)	E123K	probably benign	Het
Ttn	ATATCTCTCCAGAGCCTCCCCTGGAGGAGTGGAGTATCTCTCCAGAGCCTCCCCTGGAGGAGTGGAGTATCTCTCCAGAGCCTCCCCTG	ATATCTCTCCAGAGCCTCCCCTGGAGGAGTGGAGTATCTCTCCAGAGCCTCCCCTG	2: 76,746,150 (GRCm39)		probably benign	Het
U2surp	A	G	9: 95,375,770 (GRCm39)	V141A	probably benign	Het
Unc79	T	A	12: 103,100,734 (GRCm39)	S2050T	possibly damaging	Het
Usp13	A	G	3: 32,920,020 (GRCm39)	D235G	probably benign	Het
Vcam1	T	C	3: 115,919,628 (GRCm39)	T213A	possibly damaging	Het
Vmn2r60	T	A	7: 41,786,166 (GRCm39)	I323N	possibly damaging	Het
Vps13b	A	G	15: 35,877,703 (GRCm39)	I2892M	probably damaging	Het
Wnt5a	T	A	14: 28,240,329 (GRCm39)	S160T	probably benign	Het
Zar1	G	A	5: 72,738,294 (GRCm39)	P36L	possibly damaging	Het
Zfp773	A	T	7: 7,135,984 (GRCm39)	M204K	probably benign	Het
Zhx1	T	C	15: 57,916,465 (GRCm39)	T594A	probably benign	Het

Other mutations in Or10a2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02230:Or10a2	APN	7	106,673,700 (GRCm39)	missense	probably damaging	0.98
IGL02562:Or10a2	APN	7	106,673,769 (GRCm39)	missense	probably benign	0.03
IGL02597:Or10a2	APN	7	106,673,646 (GRCm39)	missense	possibly damaging	0.89
IGL02598:Or10a2	APN	7	106,673,923 (GRCm39)	missense	possibly damaging	0.64
IGL02981:Or10a2	APN	7	106,673,758 (GRCm39)	missense	probably damaging	1.00
BB004:Or10a2	UTSW	7	106,673,496 (GRCm39)	missense	probably benign	0.00
BB014:Or10a2	UTSW	7	106,673,496 (GRCm39)	missense	probably benign	0.00
R0064:Or10a2	UTSW	7	106,673,487 (GRCm39)	missense	probably benign	0.02
R0064:Or10a2	UTSW	7	106,673,487 (GRCm39)	missense	probably benign	0.02
R0518:Or10a2	UTSW	7	106,673,965 (GRCm39)	missense	possibly damaging	0.81
R0521:Or10a2	UTSW	7	106,673,965 (GRCm39)	missense	possibly damaging	0.81
R1661:Or10a2	UTSW	7	106,673,481 (GRCm39)	missense	probably damaging	1.00
R1665:Or10a2	UTSW	7	106,673,481 (GRCm39)	missense	probably damaging	1.00
R2069:Or10a2	UTSW	7	106,673,826 (GRCm39)	nonsense	probably null
R2202:Or10a2	UTSW	7	106,673,523 (GRCm39)	missense	probably damaging	1.00
R3884:Or10a2	UTSW	7	106,673,110 (GRCm39)	missense	possibly damaging	0.72
R4362:Or10a2	UTSW	7	106,673,799 (GRCm39)	missense	probably damaging	0.99
R4618:Or10a2	UTSW	7	106,673,761 (GRCm39)	missense	probably damaging	1.00
R5375:Or10a2	UTSW	7	106,673,080 (GRCm39)	missense	probably benign	0.05
R5654:Or10a2	UTSW	7	106,673,394 (GRCm39)	missense	probably damaging	1.00
R6228:Or10a2	UTSW	7	106,673,343 (GRCm39)	missense	probably damaging	1.00
R7196:Or10a2	UTSW	7	106,673,935 (GRCm39)	missense	probably benign	0.01
R7202:Or10a2	UTSW	7	106,673,448 (GRCm39)	missense	probably benign	0.01
R7927:Or10a2	UTSW	7	106,673,496 (GRCm39)	missense	probably benign	0.00
R9157:Or10a2	UTSW	7	106,673,214 (GRCm39)	missense	probably damaging	1.00
R9526:Or10a2	UTSW	7	106,673,739 (GRCm39)	nonsense	probably null
R9629:Or10a2	UTSW	7	106,673,164 (GRCm39)	missense	probably damaging	1.00
Z1088:Or10a2	UTSW	7	106,673,612 (GRCm39)	missense	possibly damaging	0.84

Predicted Primers

PCR Primer
(F):5'- AATGTCTCTGCTTATGCACTAGG -3'
(R):5'- TGGGCAGAATGACTAGGTTG -3'

Sequencing Primer
(F):5'- GCTTATGCACTAGGATTGGTCCTC -3'
(R):5'- ATGACTAGGTTGAAGCCGATCTCC -3'

Posted On

2019-06-26