Mutagenetix > Incidental Mutation

Incidental Mutation 'R0579:Ifna9'

56351

Institutional Source

Beutler Lab

Gene Symbol

Ifna9

Ensembl Gene

ENSMUSG00000095270

Gene Name

interferon alpha 9

Synonyms

Ifa9

MMRRC Submission

038769-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.057) question?

Stock #

R0579 (G1)

Quality Score

195

Status

Validated

Chromosome

Chromosomal Location

88510050-88510622 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 88510508 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Serine at position 39 (T39S)

Ref Sequence

ENSEMBL: ENSMUSP00000099871 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000102807]

AlphaFold

P09235

Predicted Effect

possibly damaging
Transcript: ENSMUST00000102807
AA Change: T39S

PolyPhen 2 Score 0.837 (Sensitivity: 0.84; Specificity: 0.93)

SMART Domains

Protein: ENSMUSP00000099871
Gene: ENSMUSG00000095270
AA Change: T39S

Domain	Start	End	E-Value	Type
signal peptide	1	23	N/A	INTRINSIC
IFabd	58	175	1.68e-67	SMART

Meta Mutation Damage Score

0.1795

Coding Region Coverage

1x: 99.4%
3x: 98.9%
10x: 97.4%
20x: 94.7%

Validation Efficiency

89% (34/38)

Allele List at MGI

Other mutations in this stock

Total: 39 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4921517D22Rik	GCC	GC	13: 59,839,412 (GRCm39)		probably null	Het
Abcf3	G	A	16: 20,369,398 (GRCm39)	R260Q	probably benign	Het
Abcg3	A	G	5: 105,121,969 (GRCm39)	V136A	probably damaging	Het
Acr	C	G	15: 89,453,678 (GRCm39)	H72Q	probably damaging	Het
Ambra1	A	G	2: 91,654,810 (GRCm39)	N783S	possibly damaging	Het
Cd300ld2	A	G	11: 114,903,125 (GRCm39)	F240S	probably benign	Het
Cep83	A	G	10: 94,584,915 (GRCm39)	D340G	possibly damaging	Het
Crybg2	T	A	4: 133,800,049 (GRCm39)	I403N	probably damaging	Het
Dnah14	T	A	1: 181,572,312 (GRCm39)	M2881K	possibly damaging	Het
Erbb4	T	C	1: 68,081,621 (GRCm39)	M1138V	probably benign	Het
Evi5	A	G	5: 107,969,575 (GRCm39)	V112A	probably benign	Het
F2r	A	G	13: 95,754,857 (GRCm39)	V9A	probably benign	Het
Flot1	C	A	17: 36,141,900 (GRCm39)	S337R	probably benign	Het
Glt28d2	G	A	3: 85,779,440 (GRCm39)	T11I	probably damaging	Het
Gm19345	A	G	7: 19,588,901 (GRCm39)		probably benign	Het
Gm6605	C	A	7: 38,147,699 (GRCm39)		noncoding transcript	Het
Hmgcs2	A	T	3: 98,198,264 (GRCm39)	I56F	probably damaging	Het
Il21	T	G	3: 37,281,923 (GRCm39)	K74Q	possibly damaging	Het
Itpripl1	G	T	2: 126,983,011 (GRCm39)	Y370*	probably null	Het
Kif24	G	A	4: 41,393,706 (GRCm39)	P1056S	probably damaging	Het
L2hgdh	A	T	12: 69,748,046 (GRCm39)		probably benign	Het
Lipo2	A	T	19: 33,724,298 (GRCm39)	L156Q	probably damaging	Het
Nlrp4c	T	A	7: 6,063,844 (GRCm39)	M84K	probably benign	Het
Npy4r	G	A	14: 33,868,640 (GRCm39)	T216I	probably benign	Het
Or12d17	T	C	17: 37,777,238 (GRCm39)	V47A	probably benign	Het
Or2h2c	G	C	17: 37,422,347 (GRCm39)	L176V	probably benign	Het
Or6c1	A	T	10: 129,518,106 (GRCm39)	C167*	probably null	Het
Pafah1b2	T	C	9: 45,880,011 (GRCm39)	E222G	probably benign	Het
Pop1	T	A	15: 34,510,115 (GRCm39)	D406E	possibly damaging	Het
Proser1	A	G	3: 53,374,572 (GRCm39)	Y32C	probably damaging	Het
Ptprj	C	A	2: 90,266,913 (GRCm39)		probably null	Het
Slc1a3	T	A	15: 8,717,793 (GRCm39)	I100F	probably damaging	Het
Slc25a22	T	C	7: 141,011,272 (GRCm39)	D176G	probably damaging	Het
Stard7	T	C	2: 127,126,473 (GRCm39)	V99A	probably damaging	Het
Stk33	C	T	7: 108,924,904 (GRCm39)	V184I	probably damaging	Het
Timmdc1	A	G	16: 38,342,745 (GRCm39)	L51P	probably benign	Het
Tppp	T	C	13: 74,169,352 (GRCm39)	S31P	probably benign	Het
Upf2	A	T	2: 5,993,240 (GRCm39)	R599W	unknown	Het
Vav1	G	T	17: 57,586,271 (GRCm39)	W25L	probably benign	Het

Other mutations in Ifna9

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01788:Ifna9	APN	4	88,510,097 (GRCm39)	missense	probably damaging	1.00
IGL02268:Ifna9	APN	4	88,510,591 (GRCm39)	nonsense	probably null
R1530:Ifna9	UTSW	4	88,510,409 (GRCm39)	missense	possibly damaging	0.83
R1663:Ifna9	UTSW	4	88,510,220 (GRCm39)	missense	probably benign	0.00
R1872:Ifna9	UTSW	4	88,510,492 (GRCm39)	missense	probably damaging	0.97
R3923:Ifna9	UTSW	4	88,510,508 (GRCm39)	missense	possibly damaging	0.84
R4534:Ifna9	UTSW	4	88,510,285 (GRCm39)	missense	possibly damaging	0.76
R4882:Ifna9	UTSW	4	88,510,540 (GRCm39)	missense	probably benign	0.01
R5440:Ifna9	UTSW	4	88,510,048 (GRCm39)	splice site	probably null
R6008:Ifna9	UTSW	4	88,510,600 (GRCm39)	missense	probably null	1.00
R6464:Ifna9	UTSW	4	88,510,487 (GRCm39)	missense	possibly damaging	0.93
R6584:Ifna9	UTSW	4	88,510,375 (GRCm39)	missense	probably damaging	0.99
R6899:Ifna9	UTSW	4	88,510,300 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- CAAGCAGCAGATGAGTCCTTTGATGTG -3'
(R):5'- TACCCAGACCCAAGCAGAGAATGTGG -3'

Sequencing Primer
(F):5'- CAGATGAGTCCTTTGATGTGAAGAG -3'
(R):5'- CTACATAGGATGGTCTTCAGAGAACC -3'

Posted On

2013-07-11