Mutagenetix > Incidental Mutation

Incidental Mutation 'R0580:Vmn2r96'

56407

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r96

Ensembl Gene

ENSMUSG00000091679

Gene Name

vomeronasal 2, receptor 96

Synonyms

EG433070

MMRRC Submission

038770-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.157) question?

Stock #

R0580 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

18793282-18818419 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 18802900 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Valine to Alanine at position 270 (V270A)

Ref Sequence

ENSEMBL: ENSMUSP00000156012 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000165692] [ENSMUST00000177244] [ENSMUST00000231261] [ENSMUST00000231286]

AlphaFold

E9PZU5

Predicted Effect

possibly damaging
Transcript: ENSMUST00000165692
AA Change: V78A

PolyPhen 2 Score 0.936 (Sensitivity: 0.80; Specificity: 0.94)

SMART Domains

Protein: ENSMUSP00000131564
Gene: ENSMUSG00000091679
AA Change: V78A

Domain	Start	End	E-Value	Type
Pfam:ANF_receptor	1	257	8.6e-20	PFAM
Pfam:NCD3G	317	370	3.7e-22	PFAM
Pfam:7tm_3	402	638	3.9e-53	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000177244
AA Change: V270A

PolyPhen 2 Score 0.997 (Sensitivity: 0.41; Specificity: 0.98)

SMART Domains

Protein: ENSMUSP00000135095
Gene: ENSMUSG00000091679
AA Change: V270A

Domain	Start	End	E-Value	Type
Pfam:ANF_receptor	1	245	4.2e-19	PFAM
Pfam:NCD3G	317	370	6.9e-21	PFAM
Pfam:7tm_3	400	639	3.4e-75	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000231261

Predicted Effect

probably damaging
Transcript: ENSMUST00000231286
AA Change: V270A

PolyPhen 2 Score 0.997 (Sensitivity: 0.41; Specificity: 0.98)

Meta Mutation Damage Score

0.6467

Coding Region Coverage

1x: 99.4%
3x: 98.8%
10x: 97.2%
20x: 93.7%

Validation Efficiency

95% (40/42)

Allele List at MGI

Other mutations in this stock

Total: 38 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Aadacl2fm3	T	A	3: 59,784,470 (GRCm39)	D314E	probably damaging	Het
Abcc10	C	T	17: 46,616,882 (GRCm39)		probably null	Het
Adgrg5	T	C	8: 95,663,972 (GRCm39)		probably null	Het
Akap12	A	T	10: 4,304,741 (GRCm39)	D517V	possibly damaging	Het
Arhgap23	AGAGGAGGAGGAGGAGG	AGAGGAGGAGGAGG	11: 97,337,362 (GRCm39)		probably null	Het
Bpi	A	T	2: 158,100,215 (GRCm39)	M1L	probably damaging	Het
Carm1	C	T	9: 21,494,880 (GRCm39)	P339S	probably damaging	Het
Chchd3	A	C	6: 32,870,325 (GRCm39)		probably null	Het
Chd9	T	C	8: 91,721,191 (GRCm39)	V520A	possibly damaging	Het
Chmp7	C	T	14: 69,956,899 (GRCm39)	M336I	probably benign	Het
Col10a1	A	G	10: 34,270,948 (GRCm39)	R307G	probably benign	Het
Cpeb3	T	C	19: 37,151,435 (GRCm39)	T314A	probably benign	Het
Csmd1	A	T	8: 15,960,528 (GRCm39)	Y3296N	probably damaging	Het
Dtx2	A	T	5: 136,061,180 (GRCm39)	T521S	probably damaging	Het
Ext2	A	T	2: 93,626,070 (GRCm39)	V330E	probably benign	Het
Extl3	A	G	14: 65,313,178 (GRCm39)	L668P	probably damaging	Het
Gm6605	C	A	7: 38,147,699 (GRCm39)		noncoding transcript	Het
Grm8	A	G	6: 27,761,370 (GRCm39)		probably benign	Het
Herc2	T	A	7: 55,788,539 (GRCm39)	V1763D	probably damaging	Het
Ino80	G	A	2: 119,213,962 (GRCm39)	P1203S	probably damaging	Het
Iqce	A	G	5: 140,651,156 (GRCm39)	F768L	possibly damaging	Het
Kntc1	G	A	5: 123,941,732 (GRCm39)	V1809I	probably benign	Het
Lyzl1	A	C	18: 4,181,134 (GRCm39)	T58P	probably damaging	Het
Mlxipl	A	G	5: 135,152,829 (GRCm39)	T287A	probably benign	Het
Mroh2a	G	A	1: 88,171,672 (GRCm39)	R770Q	probably damaging	Het
Net1	A	G	13: 3,936,612 (GRCm39)	Y264H	probably damaging	Het
Nnmt	T	C	9: 48,503,600 (GRCm39)	D142G	probably damaging	Het
Nod2	T	A	8: 89,391,034 (GRCm39)	I432N	probably damaging	Het
Or2h2c	G	C	17: 37,422,347 (GRCm39)	L176V	probably benign	Het
Or2y1g	T	A	11: 49,171,449 (GRCm39)	I158N	probably damaging	Het
Or6b6	A	G	7: 106,571,447 (GRCm39)	Y35H	probably damaging	Het
Pign	A	C	1: 105,519,419 (GRCm39)	I501S	probably benign	Het
Scgb2b19	A	T	7: 32,977,995 (GRCm39)	S101T	probably benign	Het
Slc7a5	A	T	8: 122,611,855 (GRCm39)	M391K	probably benign	Het
Sptan1	A	G	2: 29,897,587 (GRCm39)	R1217G	probably damaging	Het
Srgap2	A	G	1: 131,277,239 (GRCm39)	V336A	possibly damaging	Het
Srsf11	C	T	3: 157,717,704 (GRCm39)		probably benign	Het
Syt10	A	T	15: 89,711,379 (GRCm39)	D51E	probably benign	Het

Other mutations in Vmn2r96

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00562:Vmn2r96	APN	17	18,804,077 (GRCm39)	missense	probably benign	0.00
IGL00563:Vmn2r96	APN	17	18,804,077 (GRCm39)	missense	probably benign	0.00
IGL00769:Vmn2r96	APN	17	18,804,081 (GRCm39)	missense	probably benign	0.00
IGL01314:Vmn2r96	APN	17	18,803,226 (GRCm39)	missense	probably benign	0.00
IGL01983:Vmn2r96	APN	17	18,817,527 (GRCm39)	missense	probably damaging	0.99
IGL02314:Vmn2r96	APN	17	18,804,221 (GRCm39)	missense	probably benign	0.01
IGL02600:Vmn2r96	APN	17	18,817,829 (GRCm39)	missense	probably benign
IGL02672:Vmn2r96	APN	17	18,818,376 (GRCm39)	missense	probably benign	0.02
IGL02750:Vmn2r96	APN	17	18,802,851 (GRCm39)	missense	probably benign	0.01
IGL03068:Vmn2r96	APN	17	18,803,137 (GRCm39)	missense	probably benign	0.00
IGL03411:Vmn2r96	APN	17	18,806,634 (GRCm39)	missense	possibly damaging	0.88
R0316:Vmn2r96	UTSW	17	18,802,827 (GRCm39)	missense	probably damaging	1.00
R0502:Vmn2r96	UTSW	17	18,804,262 (GRCm39)	missense	probably benign
R0652:Vmn2r96	UTSW	17	18,817,830 (GRCm39)	missense	probably benign	0.05
R0789:Vmn2r96	UTSW	17	18,802,738 (GRCm39)	missense	possibly damaging	0.81
R1462:Vmn2r96	UTSW	17	18,817,660 (GRCm39)	missense	possibly damaging	0.94
R1462:Vmn2r96	UTSW	17	18,817,660 (GRCm39)	missense	possibly damaging	0.94
R1660:Vmn2r96	UTSW	17	18,817,988 (GRCm39)	missense	probably benign	0.00
R1755:Vmn2r96	UTSW	17	18,802,915 (GRCm39)	missense	possibly damaging	0.50
R1843:Vmn2r96	UTSW	17	18,818,183 (GRCm39)	missense	probably benign	0.14
R1943:Vmn2r96	UTSW	17	18,806,664 (GRCm39)	missense	probably benign	0.09
R1993:Vmn2r96	UTSW	17	18,804,138 (GRCm39)	missense	probably damaging	1.00
R2018:Vmn2r96	UTSW	17	18,804,263 (GRCm39)	missense	probably benign
R2405:Vmn2r96	UTSW	17	18,818,102 (GRCm39)	missense	probably damaging	0.96
R3977:Vmn2r96	UTSW	17	18,817,941 (GRCm39)	missense	probably damaging	1.00
R3979:Vmn2r96	UTSW	17	18,817,941 (GRCm39)	missense	probably damaging	1.00
R4059:Vmn2r96	UTSW	17	18,818,339 (GRCm39)	missense	probably benign	0.20
R4693:Vmn2r96	UTSW	17	18,803,270 (GRCm39)	missense	probably benign	0.03
R4709:Vmn2r96	UTSW	17	18,803,088 (GRCm39)	missense	probably benign	0.36
R4776:Vmn2r96	UTSW	17	18,817,770 (GRCm39)	missense	probably damaging	1.00
R4882:Vmn2r96	UTSW	17	18,817,866 (GRCm39)	missense	probably damaging	1.00
R4920:Vmn2r96	UTSW	17	18,802,918 (GRCm39)	missense	probably benign	0.07
R5143:Vmn2r96	UTSW	17	18,804,120 (GRCm39)	missense	possibly damaging	0.65
R5301:Vmn2r96	UTSW	17	18,817,950 (GRCm39)	missense	probably damaging	0.98
R5507:Vmn2r96	UTSW	17	18,818,091 (GRCm39)	missense	probably damaging	1.00
R6151:Vmn2r96	UTSW	17	18,804,221 (GRCm39)	missense	probably benign	0.01
R6181:Vmn2r96	UTSW	17	18,804,126 (GRCm39)	missense	probably benign
R6339:Vmn2r96	UTSW	17	18,804,124 (GRCm39)	missense	possibly damaging	0.84
R6404:Vmn2r96	UTSW	17	18,817,793 (GRCm39)	missense	probably damaging	1.00
R6452:Vmn2r96	UTSW	17	18,804,117 (GRCm39)	missense	probably benign	0.07
R6749:Vmn2r96	UTSW	17	18,818,352 (GRCm39)	missense	probably damaging	0.99
R6813:Vmn2r96	UTSW	17	18,802,116 (GRCm39)	missense	probably benign	0.04
R6851:Vmn2r96	UTSW	17	18,802,800 (GRCm39)	missense	possibly damaging	0.95
R6944:Vmn2r96	UTSW	17	18,817,891 (GRCm39)	missense	probably benign	0.16
R6949:Vmn2r96	UTSW	17	18,818,100 (GRCm39)	missense	probably damaging	1.00
R6962:Vmn2r96	UTSW	17	18,818,283 (GRCm39)	missense	probably damaging	0.99
R6990:Vmn2r96	UTSW	17	18,804,082 (GRCm39)	missense	probably benign
R7149:Vmn2r96	UTSW	17	18,817,989 (GRCm39)	missense	possibly damaging	0.89
R7346:Vmn2r96	UTSW	17	18,803,029 (GRCm39)	missense	probably benign	0.15
R7385:Vmn2r96	UTSW	17	18,803,302 (GRCm39)	missense	probably damaging	1.00
R7442:Vmn2r96	UTSW	17	18,793,662 (GRCm39)	missense	probably benign	0.02
R7509:Vmn2r96	UTSW	17	18,802,995 (GRCm39)	missense	probably benign	0.02
R7652:Vmn2r96	UTSW	17	18,793,832 (GRCm39)	missense	probably benign
R7659:Vmn2r96	UTSW	17	18,793,749 (GRCm39)	missense	probably benign	0.00
R7753:Vmn2r96	UTSW	17	18,806,663 (GRCm39)	missense	possibly damaging	0.46
R7855:Vmn2r96	UTSW	17	18,818,130 (GRCm39)	missense	possibly damaging	0.59
R8166:Vmn2r96	UTSW	17	18,802,744 (GRCm39)	missense	probably damaging	1.00
R8260:Vmn2r96	UTSW	17	18,804,243 (GRCm39)	missense	probably benign	0.04
R8323:Vmn2r96	UTSW	17	18,803,023 (GRCm39)	missense	probably damaging	0.99
R8787:Vmn2r96	UTSW	17	18,818,250 (GRCm39)	missense	probably damaging	1.00
R8837:Vmn2r96	UTSW	17	18,802,888 (GRCm39)	missense	probably benign
R8933:Vmn2r96	UTSW	17	18,804,241 (GRCm39)	missense	probably benign	0.11
R9306:Vmn2r96	UTSW	17	18,803,226 (GRCm39)	missense	probably benign	0.00
R9481:Vmn2r96	UTSW	17	18,793,621 (GRCm39)	start gained	probably benign
R9626:Vmn2r96	UTSW	17	18,793,758 (GRCm39)	missense	probably benign	0.14
R9629:Vmn2r96	UTSW	17	18,803,257 (GRCm39)	missense	probably benign	0.15
Z1088:Vmn2r96	UTSW	17	18,817,628 (GRCm39)	missense	possibly damaging	0.86
Z1177:Vmn2r96	UTSW	17	18,818,376 (GRCm39)	missense	probably benign	0.02

Predicted Primers

PCR Primer
(F):5'- GGTTGGTCTCATCCTTCCCAATGAC -3'
(R):5'- TGGAGGCTGTAAGCCACAGCATAC -3'

Sequencing Primer
(F):5'- TCCTTCCCAATGACCACAAAG -3'
(R):5'- ATCCAAGGTAGCATTGGTTTGAC -3'

Posted On

2013-07-11