Mutagenetix > Incidental Mutation

Incidental Mutation 'R7282:Lama5'

565704

Institutional Source

Beutler Lab

Gene Symbol

Lama5

Ensembl Gene

ENSMUSG00000015647

Gene Name

laminin, alpha 5

Synonyms

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R7282 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

179818166-179867652 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 179843588 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Tyrosine to Cysteine at position 453 (Y453C)

Ref Sequence

ENSEMBL: ENSMUSP00000015791 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000015791]

AlphaFold

no structure available at present

PDB Structure

LAMININ ALPHA5 CHAIN N-TERMINAL FRAGMENT [X-RAY DIFFRACTION]

Predicted Effect

probably damaging
Transcript: ENSMUST00000015791
AA Change: Y453C

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000015791
Gene: ENSMUSG00000015647
AA Change: Y453C

Domain	Start	End	E-Value	Type
signal peptide	1	40	N/A	INTRINSIC
LamNT	44	303	1.06e-132	SMART
EGF_Lam	305	361	4.35e-6	SMART
EGF_Lam	364	431	5.78e-11	SMART
EGF_Lam	434	476	1.32e-5	SMART
EGF_Lam	500	544	8.63e-10	SMART
EGF_Lam	547	590	1.16e-10	SMART
EGF_Lam	593	635	4.63e-10	SMART
EGF_Lam	638	680	6.25e-7	SMART
EGF_Lam	683	726	3.1e-11	SMART
EGF_Lam	730	779	2.99e-4	SMART
EGF_Lam	782	831	4.66e-6	SMART
EGF_Lam	834	878	3.48e-5	SMART
low complexity region	1261	1273	N/A	INTRINSIC
EGF_Lam	1443	1486	7.01e-10	SMART
EGF_like	1489	1530	3.64e-1	SMART
EGF_Lam	1533	1579	8.56e-14	SMART
EGF_Lam	1582	1630	1.86e-14	SMART
LamB	1689	1819	5.86e-61	SMART
EGF_like	1818	1862	2.74e0	SMART
EGF_Lam	1865	1912	3.32e-11	SMART
EGF_Lam	1915	1968	1.61e-9	SMART
EGF_Lam	1971	2022	6.39e-13	SMART
EGF_Lam	2025	2069	1.94e-12	SMART
EGF_Lam	2072	2116	1.35e-11	SMART
EGF_like	2103	2145	3.1e1	SMART
EGF_Lam	2119	2166	1.18e-2	SMART
Pfam:Laminin_I	2189	2453	1.7e-65	PFAM
low complexity region	2532	2548	N/A	INTRINSIC
low complexity region	2557	2569	N/A	INTRINSIC
low complexity region	2632	2641	N/A	INTRINSIC
low complexity region	2663	2676	N/A	INTRINSIC
LamG	2760	2912	3.97e-8	SMART
LamG	2966	3103	1.78e-10	SMART
LamG	3149	3274	1.11e-20	SMART
LamG	3359	3497	4.05e-23	SMART
LamG	3539	3670	3e-26	SMART

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 98.8%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes one of the vertebrate laminin alpha chains. Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins are composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively) and they form a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. Each laminin chain is a multidomain protein encoded by a distinct gene. The protein encoded by this gene is the alpha-5 subunit of of laminin-10 (laminin-511), laminin-11 (laminin-521) and laminin-15 (laminin-523). [provided by RefSeq, Jun 2013]
PHENOTYPE: Homozygotes for a targeted null mutation exhibit disrupted basal laminae leading to exencephaly, syndactyly, placentopathy, kidney defects, abnormal lobar septation with absence of a visceral pleural membrane, and lethality in late gestation. [provided by MGI curators]

Allele List at MGI

All alleles(49) : Targeted(5) Gene trapped(44)

Other mutations in this stock

Total: 80 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1810065E05Rik	C	A	11: 58,316,582 (GRCm39)	D187E	probably damaging	Het
Abl2	T	C	1: 156,457,630 (GRCm39)	Y299H	probably damaging	Het
Acsl3	T	A	1: 78,659,709 (GRCm39)	N120K	probably damaging	Het
Aldh1a1	A	G	19: 20,606,434 (GRCm39)	K255R	possibly damaging	Het
Baz2b	C	T	2: 59,750,781 (GRCm39)	R1205H	probably benign	Het
Btbd8	G	T	5: 107,655,062 (GRCm39)	V954L	probably benign	Het
Btbd8	A	G	5: 107,658,371 (GRCm39)	D1647G	probably damaging	Het
Carmil1	A	G	13: 24,197,387 (GRCm39)	S1350P	probably benign	Het
Cecr2	A	T	6: 120,738,582 (GRCm39)	N325I		Het
Cep120	C	T	18: 53,873,161 (GRCm39)	A57T	probably damaging	Het
Chst8	T	C	7: 34,447,628 (GRCm39)		probably null	Het
Cntn4	A	T	6: 106,502,421 (GRCm39)	I393F	probably damaging	Het
Crocc	T	C	4: 140,749,652 (GRCm39)	D1494G	probably damaging	Het
Cwc25	T	C	11: 97,638,832 (GRCm39)	E364G	possibly damaging	Het
Dnah7a	C	A	1: 53,724,059 (GRCm39)		probably null	Het
Drg2	A	G	11: 60,345,519 (GRCm39)	E5G	probably benign	Het
Ehd4	T	C	2: 119,921,729 (GRCm39)	H509R	probably damaging	Het
Enam	A	T	5: 88,650,186 (GRCm39)	N565I	probably damaging	Het
Evi2a	G	T	11: 79,418,249 (GRCm39)	N120K	probably benign	Het
Faim	A	G	9: 98,874,179 (GRCm39)	T2A	probably benign	Het
Fcgbpl1	A	G	7: 27,843,833 (GRCm39)	N907S	probably benign	Het
Fmnl1	T	C	11: 103,087,091 (GRCm39)	V836A	unknown	Het
Fosb	T	C	7: 19,039,113 (GRCm39)	I224V	possibly damaging	Het
Frem3	A	T	8: 81,338,660 (GRCm39)	T318S	probably damaging	Het
Gm9972	G	A	11: 42,927,631 (GRCm39)	G93R	unknown	Het
Gtf2a1	A	T	12: 91,534,609 (GRCm39)	I215N	possibly damaging	Het
Hs3st3b1	A	G	11: 63,812,397 (GRCm39)	V106A	probably benign	Het
Igkv19-93	T	C	6: 68,713,485 (GRCm39)	D48G	probably benign	Het
Itgb8	A	T	12: 119,201,443 (GRCm39)	W31R	probably benign	Het
Kmt2d	A	T	15: 98,751,985 (GRCm39)	W1995R	unknown	Het
Lama3	G	T	18: 12,572,449 (GRCm39)	Q551H	probably damaging	Het
Lbhd2	G	A	12: 111,376,724 (GRCm39)	R57H	probably damaging	Het
Lcmt2	A	G	2: 120,969,271 (GRCm39)	V384A	probably damaging	Het
Lgsn	T	G	1: 31,242,452 (GRCm39)	L178R	probably damaging	Het
Lhcgr	C	T	17: 89,065,811 (GRCm39)	V193I	probably benign	Het
Lta4h	T	C	10: 93,289,373 (GRCm39)	M1T	probably null	Het
Ly6l	T	C	15: 75,321,345 (GRCm39)	S14P	probably damaging	Het
Met	C	A	6: 17,547,011 (GRCm39)	C881*	probably null	Het
Mettl21e	A	T	1: 44,249,399 (GRCm39)	Y86N	probably damaging	Het
Mgam	A	G	6: 40,740,045 (GRCm39)	T1673A	probably benign	Het
Mgam	A	T	6: 40,633,446 (GRCm39)	N251Y	possibly damaging	Het
Mmp27	T	A	9: 7,578,231 (GRCm39)	V357D	probably damaging	Het
Muc2	A	G	7: 141,306,481 (GRCm39)	E740G		Het
Myoc	C	A	1: 162,476,413 (GRCm39)	S372R	probably benign	Het
Ncor2	A	T	5: 125,097,104 (GRCm39)	M1358K		Het
Nol6	A	G	4: 41,119,468 (GRCm39)	S613P	probably benign	Het
Nuggc	T	A	14: 65,855,072 (GRCm39)	I334N	probably damaging	Het
Nxnl2	C	T	13: 51,325,542 (GRCm39)	P62S	probably damaging	Het
Ogfod1	C	T	8: 94,764,067 (GRCm39)	H51Y	possibly damaging	Het
Olfml1	T	A	7: 107,189,530 (GRCm39)	D198E	possibly damaging	Het
Or2t26	A	C	11: 49,039,945 (GRCm39)	N287T	probably damaging	Het
Ovch2	C	T	7: 107,393,577 (GRCm39)	R183H	possibly damaging	Het
Plxnd1	A	T	6: 115,937,798 (GRCm39)	L1516Q	probably damaging	Het
Pramel27	A	G	4: 143,578,451 (GRCm39)	N237S	possibly damaging	Het
Prrc2c	A	G	1: 162,507,543 (GRCm39)	V2448A	possibly damaging	Het
Rexo5	C	T	7: 119,417,636 (GRCm39)	T212I	probably damaging	Het
Rgl2	C	T	17: 34,152,403 (GRCm39)	R367W	probably damaging	Het
Rnf213	T	G	11: 119,328,818 (GRCm39)	I2030S		Het
Rtf1	C	T	2: 119,505,580 (GRCm39)	A11V	unknown	Het
Setdb1	T	G	3: 95,245,985 (GRCm39)	T647P	probably damaging	Het
Shisa6	G	C	11: 66,393,480 (GRCm39)	P272R	possibly damaging	Het
Slc25a54	G	T	3: 109,023,817 (GRCm39)	G471*	probably null	Het
Slc30a1	A	G	1: 191,641,544 (GRCm39)	T397A	probably benign	Het
Slc7a14	A	G	3: 31,281,302 (GRCm39)	F336S	possibly damaging	Het
Slitrk3	C	T	3: 72,957,798 (GRCm39)	V325I	possibly damaging	Het
Sptan1	A	G	2: 29,876,941 (GRCm39)	Y361C	probably damaging	Het
Stard9	A	G	2: 120,528,984 (GRCm39)	D1747G	probably benign	Het
Taf3	T	C	2: 9,956,253 (GRCm39)	E638G	probably damaging	Het
Tbc1d8	T	C	1: 39,411,614 (GRCm39)	D1074G	probably benign	Het
Tecrl	G	A	5: 83,502,754 (GRCm39)	H32Y	probably benign	Het
Tgm3	T	A	2: 129,866,481 (GRCm39)	M133K	probably benign	Het
Tmem131	A	G	1: 36,880,685 (GRCm39)	F195S	probably damaging	Het
Tmem82	T	C	4: 141,342,261 (GRCm39)	I314V	possibly damaging	Het
Trappc10	T	C	10: 78,043,327 (GRCm39)	E555G	probably damaging	Het
Ubn2	A	T	6: 38,429,811 (GRCm39)	K176*	probably null	Het
Ucp1	G	A	8: 84,020,531 (GRCm39)	G114R	probably benign	Het
Unc5c	A	G	3: 141,383,751 (GRCm39)	D43G	probably damaging	Het
Vmn2r3	C	T	3: 64,168,825 (GRCm39)	V571M	possibly damaging	Het
Vwa3a	A	T	7: 120,385,688 (GRCm39)	I677L	probably benign	Het
Wdr48	T	C	9: 119,740,147 (GRCm39)	S319P	probably damaging	Het

Other mutations in Lama5

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01067:Lama5	APN	2	179,818,336 (GRCm39)	unclassified	probably benign
IGL01370:Lama5	APN	2	179,839,193 (GRCm39)	missense	possibly damaging	0.87
IGL01474:Lama5	APN	2	179,838,363 (GRCm39)	missense	probably damaging	1.00
IGL01614:Lama5	APN	2	179,822,657 (GRCm39)	missense	probably damaging	1.00
IGL01941:Lama5	APN	2	179,834,185 (GRCm39)	missense	possibly damaging	0.71
IGL01953:Lama5	APN	2	179,832,497 (GRCm39)	missense	probably damaging	0.97
IGL02093:Lama5	APN	2	179,830,380 (GRCm39)	missense	probably damaging	1.00
IGL02197:Lama5	APN	2	179,849,012 (GRCm39)	missense	possibly damaging	0.82
IGL02308:Lama5	APN	2	179,832,120 (GRCm39)	splice site	probably benign
IGL02314:Lama5	APN	2	179,836,275 (GRCm39)	splice site	probably benign
IGL02317:Lama5	APN	2	179,833,112 (GRCm39)	missense	probably damaging	1.00
IGL02354:Lama5	APN	2	179,835,677 (GRCm39)	nonsense	probably null
IGL02361:Lama5	APN	2	179,835,677 (GRCm39)	nonsense	probably null
IGL02557:Lama5	APN	2	179,832,725 (GRCm39)	nonsense	probably null
IGL03026:Lama5	APN	2	179,837,760 (GRCm39)	missense	probably benign	0.34
IGL03160:Lama5	APN	2	179,822,128 (GRCm39)	missense	probably damaging	1.00
IGL03238:Lama5	APN	2	179,830,367 (GRCm39)	missense	probably benign
IGL03390:Lama5	APN	2	179,849,011 (GRCm39)	missense	probably damaging	1.00
blancmange	UTSW	2	179,822,404 (GRCm39)	missense	probably damaging	0.98
cupcake	UTSW	2	179,827,752 (GRCm39)	missense	probably damaging	1.00
layercake	UTSW	2	179,822,511 (GRCm39)	missense	possibly damaging	0.83
poundcake	UTSW	2	179,837,401 (GRCm39)	missense	probably damaging	1.00
Salty	UTSW	2	179,823,444 (GRCm39)	missense	possibly damaging	0.84
PIT4378001:Lama5	UTSW	2	179,831,238 (GRCm39)	missense	possibly damaging	0.89
R0003:Lama5	UTSW	2	179,819,872 (GRCm39)	splice site	probably null
R0056:Lama5	UTSW	2	179,828,899 (GRCm39)	intron	probably benign
R0147:Lama5	UTSW	2	179,832,199 (GRCm39)	missense	probably benign
R0148:Lama5	UTSW	2	179,832,199 (GRCm39)	missense	probably benign
R0310:Lama5	UTSW	2	179,823,359 (GRCm39)	splice site	probably benign
R0326:Lama5	UTSW	2	179,824,219 (GRCm39)	missense	possibly damaging	0.90
R0368:Lama5	UTSW	2	179,823,023 (GRCm39)	nonsense	probably null
R0479:Lama5	UTSW	2	179,826,250 (GRCm39)	missense	probably benign	0.03
R0490:Lama5	UTSW	2	179,821,962 (GRCm39)	missense	possibly damaging	0.90
R0636:Lama5	UTSW	2	179,831,124 (GRCm39)	critical splice donor site	probably null
R0704:Lama5	UTSW	2	179,821,277 (GRCm39)	missense	possibly damaging	0.84
R0733:Lama5	UTSW	2	179,822,511 (GRCm39)	missense	possibly damaging	0.83
R1017:Lama5	UTSW	2	179,837,213 (GRCm39)	missense	probably damaging	1.00
R1078:Lama5	UTSW	2	179,821,557 (GRCm39)	unclassified	probably benign
R1294:Lama5	UTSW	2	179,832,714 (GRCm39)	missense	probably benign	0.00
R1423:Lama5	UTSW	2	179,837,434 (GRCm39)	missense	probably damaging	1.00
R1438:Lama5	UTSW	2	179,824,593 (GRCm39)	missense	probably benign	0.01
R1447:Lama5	UTSW	2	179,827,671 (GRCm39)	missense	probably damaging	0.99
R1540:Lama5	UTSW	2	179,821,944 (GRCm39)	missense	probably benign
R1601:Lama5	UTSW	2	179,839,538 (GRCm39)	missense	probably damaging	1.00
R1624:Lama5	UTSW	2	179,848,551 (GRCm39)	missense	probably benign	0.02
R1674:Lama5	UTSW	2	179,843,780 (GRCm39)	missense	probably benign	0.00
R1687:Lama5	UTSW	2	179,835,859 (GRCm39)	missense	probably benign	0.00
R1696:Lama5	UTSW	2	179,844,279 (GRCm39)	missense	probably damaging	1.00
R1701:Lama5	UTSW	2	179,863,162 (GRCm39)	missense	probably damaging	1.00
R1778:Lama5	UTSW	2	179,837,274 (GRCm39)	splice site	probably benign
R1936:Lama5	UTSW	2	179,832,714 (GRCm39)	missense	probably benign	0.00
R1939:Lama5	UTSW	2	179,832,714 (GRCm39)	missense	probably benign	0.00
R1940:Lama5	UTSW	2	179,832,714 (GRCm39)	missense	probably benign	0.00
R1953:Lama5	UTSW	2	179,832,540 (GRCm39)	missense	possibly damaging	0.94
R1966:Lama5	UTSW	2	179,830,145 (GRCm39)	missense	probably damaging	1.00
R2024:Lama5	UTSW	2	179,820,923 (GRCm39)	missense	probably benign	0.00
R2079:Lama5	UTSW	2	179,867,301 (GRCm39)	missense	possibly damaging	0.68
R2115:Lama5	UTSW	2	179,828,678 (GRCm39)	missense	probably damaging	1.00
R2173:Lama5	UTSW	2	179,838,035 (GRCm39)	missense	probably benign	0.00
R2272:Lama5	UTSW	2	179,820,396 (GRCm39)	missense	possibly damaging	0.93
R2357:Lama5	UTSW	2	179,821,890 (GRCm39)	missense	probably benign	0.01
R2860:Lama5	UTSW	2	179,829,040 (GRCm39)	missense	probably benign	0.00
R2861:Lama5	UTSW	2	179,829,040 (GRCm39)	missense	probably benign	0.00
R2939:Lama5	UTSW	2	179,840,747 (GRCm39)	missense	probably damaging	1.00
R3053:Lama5	UTSW	2	179,824,860 (GRCm39)	missense	probably damaging	0.99
R3430:Lama5	UTSW	2	179,838,110 (GRCm39)	missense	probably benign	0.00
R3752:Lama5	UTSW	2	179,829,015 (GRCm39)	missense	probably damaging	1.00
R3782:Lama5	UTSW	2	179,836,356 (GRCm39)	missense	possibly damaging	0.57
R3901:Lama5	UTSW	2	179,824,144 (GRCm39)	splice site	probably benign
R4248:Lama5	UTSW	2	179,822,220 (GRCm39)	missense	possibly damaging	0.84
R4626:Lama5	UTSW	2	179,826,253 (GRCm39)	missense	probably damaging	0.98
R4638:Lama5	UTSW	2	179,832,206 (GRCm39)	missense	possibly damaging	0.89
R4669:Lama5	UTSW	2	179,822,430 (GRCm39)	missense	probably damaging	1.00
R4673:Lama5	UTSW	2	179,841,059 (GRCm39)	missense	probably damaging	1.00
R4677:Lama5	UTSW	2	179,821,159 (GRCm39)	missense	possibly damaging	0.69
R4701:Lama5	UTSW	2	179,833,489 (GRCm39)	missense	probably damaging	1.00
R4774:Lama5	UTSW	2	179,827,734 (GRCm39)	missense	probably damaging	1.00
R4880:Lama5	UTSW	2	179,818,861 (GRCm39)	unclassified	probably benign
R4923:Lama5	UTSW	2	179,825,942 (GRCm39)	missense	probably benign	0.18
R4960:Lama5	UTSW	2	179,850,045 (GRCm39)	critical splice donor site	probably null
R4983:Lama5	UTSW	2	179,835,242 (GRCm39)	missense	probably benign	0.13
R5061:Lama5	UTSW	2	179,840,579 (GRCm39)	nonsense	probably null
R5080:Lama5	UTSW	2	179,848,993 (GRCm39)	nonsense	probably null
R5135:Lama5	UTSW	2	179,844,013 (GRCm39)	missense	possibly damaging	0.89
R5206:Lama5	UTSW	2	179,833,097 (GRCm39)	missense	probably damaging	1.00
R5296:Lama5	UTSW	2	179,835,594 (GRCm39)	missense	probably damaging	1.00
R5319:Lama5	UTSW	2	179,822,911 (GRCm39)	missense	probably damaging	1.00
R5355:Lama5	UTSW	2	179,823,444 (GRCm39)	missense	possibly damaging	0.84
R5388:Lama5	UTSW	2	179,832,539 (GRCm39)	missense	possibly damaging	0.83
R5528:Lama5	UTSW	2	179,836,356 (GRCm39)	missense	probably benign	0.21
R5536:Lama5	UTSW	2	179,831,142 (GRCm39)	missense	probably damaging	0.99
R5658:Lama5	UTSW	2	179,850,069 (GRCm39)	nonsense	probably null
R5823:Lama5	UTSW	2	179,834,285 (GRCm39)	missense	probably benign	0.04
R5885:Lama5	UTSW	2	179,843,624 (GRCm39)	missense	probably damaging	1.00
R5889:Lama5	UTSW	2	179,835,467 (GRCm39)	intron	probably benign
R5912:Lama5	UTSW	2	179,837,268 (GRCm39)	missense	probably damaging	1.00
R5955:Lama5	UTSW	2	179,839,267 (GRCm39)	missense	probably damaging	1.00
R6015:Lama5	UTSW	2	179,827,185 (GRCm39)	missense	probably benign	0.36
R6037:Lama5	UTSW	2	179,848,806 (GRCm39)	missense	probably damaging	1.00
R6037:Lama5	UTSW	2	179,848,806 (GRCm39)	missense	probably damaging	1.00
R6191:Lama5	UTSW	2	179,827,752 (GRCm39)	missense	probably damaging	1.00
R6191:Lama5	UTSW	2	179,822,404 (GRCm39)	missense	probably damaging	0.98
R6359:Lama5	UTSW	2	179,837,775 (GRCm39)	missense	probably benign	0.01
R6385:Lama5	UTSW	2	179,838,326 (GRCm39)	missense	probably damaging	1.00
R6406:Lama5	UTSW	2	179,839,257 (GRCm39)	nonsense	probably null
R6552:Lama5	UTSW	2	179,822,947 (GRCm39)	missense	probably damaging	0.98
R6632:Lama5	UTSW	2	179,833,455 (GRCm39)	missense	probably damaging	1.00
R6633:Lama5	UTSW	2	179,833,455 (GRCm39)	missense	probably damaging	1.00
R6645:Lama5	UTSW	2	179,821,463 (GRCm39)	missense	probably damaging	1.00
R6731:Lama5	UTSW	2	179,830,367 (GRCm39)	missense	probably benign	0.09
R6744:Lama5	UTSW	2	179,833,455 (GRCm39)	missense	probably damaging	1.00
R6798:Lama5	UTSW	2	179,833,455 (GRCm39)	missense	probably damaging	1.00
R6799:Lama5	UTSW	2	179,833,455 (GRCm39)	missense	probably damaging	1.00
R6801:Lama5	UTSW	2	179,833,455 (GRCm39)	missense	probably damaging	1.00
R6851:Lama5	UTSW	2	179,833,455 (GRCm39)	missense	probably damaging	1.00
R6869:Lama5	UTSW	2	179,833,455 (GRCm39)	missense	probably damaging	1.00
R6881:Lama5	UTSW	2	179,833,455 (GRCm39)	missense	probably damaging	1.00
R6882:Lama5	UTSW	2	179,833,455 (GRCm39)	missense	probably damaging	1.00
R6884:Lama5	UTSW	2	179,833,455 (GRCm39)	missense	probably damaging	1.00
R7022:Lama5	UTSW	2	179,822,524 (GRCm39)	missense	probably damaging	1.00
R7204:Lama5	UTSW	2	179,843,970 (GRCm39)	missense	probably damaging	1.00
R7207:Lama5	UTSW	2	179,848,877 (GRCm39)	missense	probably damaging	0.98
R7367:Lama5	UTSW	2	179,834,751 (GRCm39)	missense	probably benign	0.01
R7410:Lama5	UTSW	2	179,844,183 (GRCm39)	critical splice donor site	probably null
R7699:Lama5	UTSW	2	179,822,654 (GRCm39)	missense	probably damaging	1.00
R7849:Lama5	UTSW	2	179,843,605 (GRCm39)	missense	probably damaging	1.00
R7909:Lama5	UTSW	2	179,834,069 (GRCm39)	missense	possibly damaging	0.95
R7948:Lama5	UTSW	2	179,843,994 (GRCm39)	missense	probably damaging	1.00
R8153:Lama5	UTSW	2	179,829,724 (GRCm39)	missense	probably benign	0.37
R8317:Lama5	UTSW	2	179,848,784 (GRCm39)	missense	probably damaging	1.00
R8351:Lama5	UTSW	2	179,837,401 (GRCm39)	missense	probably damaging	1.00
R8370:Lama5	UTSW	2	179,843,280 (GRCm39)	missense	possibly damaging	0.80
R8398:Lama5	UTSW	2	179,838,827 (GRCm39)	critical splice donor site	probably null
R8401:Lama5	UTSW	2	179,840,580 (GRCm39)	missense	probably damaging	1.00
R8404:Lama5	UTSW	2	179,837,015 (GRCm39)	missense	probably damaging	1.00
R8502:Lama5	UTSW	2	179,837,015 (GRCm39)	missense	probably damaging	1.00
R8694:Lama5	UTSW	2	179,822,677 (GRCm39)	missense	probably damaging	0.98
R8705:Lama5	UTSW	2	179,820,354 (GRCm39)	missense	probably damaging	1.00
R8732:Lama5	UTSW	2	179,828,481 (GRCm39)	missense	probably damaging	1.00
R8755:Lama5	UTSW	2	179,832,714 (GRCm39)	missense	probably benign	0.00
R8786:Lama5	UTSW	2	179,838,100 (GRCm39)	missense	probably damaging	1.00
R8926:Lama5	UTSW	2	179,835,783 (GRCm39)	missense	probably benign	0.08
R8928:Lama5	UTSW	2	179,843,832 (GRCm39)	missense	probably damaging	1.00
R8953:Lama5	UTSW	2	179,835,313 (GRCm39)	missense	probably damaging	0.99
R8958:Lama5	UTSW	2	179,835,592 (GRCm39)	missense	probably benign
R9002:Lama5	UTSW	2	179,838,311 (GRCm39)	missense	probably damaging	1.00
R9081:Lama5	UTSW	2	179,833,930 (GRCm39)	nonsense	probably null
R9165:Lama5	UTSW	2	179,821,286 (GRCm39)	missense	probably damaging	0.99
R9233:Lama5	UTSW	2	179,840,502 (GRCm39)	nonsense	probably null
R9264:Lama5	UTSW	2	179,838,271 (GRCm39)	splice site	probably benign
R9311:Lama5	UTSW	2	179,838,275 (GRCm39)	critical splice donor site	probably null
R9443:Lama5	UTSW	2	179,843,522 (GRCm39)	missense	probably benign	0.00
R9488:Lama5	UTSW	2	179,823,234 (GRCm39)	missense	possibly damaging	0.95
R9674:Lama5	UTSW	2	179,840,267 (GRCm39)	critical splice donor site	probably null
R9684:Lama5	UTSW	2	179,849,038 (GRCm39)	missense	probably damaging	1.00
R9749:Lama5	UTSW	2	179,825,433 (GRCm39)	missense	probably benign	0.00
RF020:Lama5	UTSW	2	179,837,971 (GRCm39)	missense	probably benign
X0065:Lama5	UTSW	2	179,823,524 (GRCm39)	missense	probably benign	0.26
Z1177:Lama5	UTSW	2	179,832,507 (GRCm39)	missense	possibly damaging	0.95
Z1177:Lama5	UTSW	2	179,831,212 (GRCm39)	missense	probably damaging	1.00
Z1177:Lama5	UTSW	2	179,825,423 (GRCm39)	missense	probably benign	0.03
Z1177:Lama5	UTSW	2	179,840,603 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TACTTGTCCACCAGGTCTGTG -3'
(R):5'- CTGTGTTCCTAAGCCTTGGAG -3'

Sequencing Primer
(F):5'- TCTGGCTCCAGTACCACAG -3'
(R):5'- AGGCTGCGAGTCTTAGGGATC -3'

Posted On

2019-06-26