Mutagenetix > Incidental Mutation

Incidental Mutation 'R7286:Hmgcr'

566043

Institutional Source

Beutler Lab

Gene Symbol

Hmgcr

Ensembl Gene

ENSMUSG00000021670

Gene Name

3-hydroxy-3-methylglutaryl-Coenzyme A reductase

Synonyms

Red, 3-hydroxy-3-methylglutaryl-CoA reductase, HMG-CoAR

MMRRC Submission

045394-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R7286 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

96785475-96807444 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

C to T at 96803105 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Cysteine to Tyrosine at position 30 (C30Y)

Ref Sequence

ENSEMBL: ENSMUSP00000022176 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000022176] [ENSMUST00000168855] [ENSMUST00000169196] [ENSMUST00000169966] [ENSMUST00000170287] [ENSMUST00000171537]

AlphaFold

Q01237

Predicted Effect

probably damaging
Transcript: ENSMUST00000022176
AA Change: C30Y

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000022176
Gene: ENSMUSG00000021670
AA Change: C30Y

Domain	Start	End	E-Value	Type
transmembrane domain	20	37	N/A	INTRINSIC
Pfam:Patched	56	342	2.7e-11	PFAM
Pfam:Sterol-sensing	85	234	3.4e-20	PFAM
Pfam:HMG-CoA_red	490	870	2.2e-150	PFAM

Predicted Effect

possibly damaging
Transcript: ENSMUST00000168855
AA Change: C30Y

PolyPhen 2 Score 0.827 (Sensitivity: 0.84; Specificity: 0.93)

Predicted Effect

probably damaging
Transcript: ENSMUST00000169196
AA Change: C30Y

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000132749
Gene: ENSMUSG00000021670
AA Change: C30Y

Domain	Start	End	E-Value	Type
transmembrane domain	20	37	N/A	INTRINSIC
transmembrane domain	57	79	N/A	INTRINSIC
Pfam:Sterol-sensing	85	210	4.7e-15	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000169966
AA Change: C30Y

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000128294
Gene: ENSMUSG00000021670
AA Change: C30Y

Domain	Start	End	E-Value	Type
transmembrane domain	15	37	N/A	INTRINSIC
transmembrane domain	57	79	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000170287
AA Change: C30Y

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000128939
Gene: ENSMUSG00000021670
AA Change: C30Y

Domain	Start	End	E-Value	Type
transmembrane domain	20	37	N/A	INTRINSIC
Pfam:Patched	56	347	1.4e-11	PFAM
Pfam:Sterol-sensing	85	234	7.4e-20	PFAM
Pfam:HMG-CoA_red	488	819	1.3e-148	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000171537
AA Change: C40Y

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000126959
Gene: ENSMUSG00000021670
AA Change: C40Y

Domain	Start	End	E-Value	Type
transmembrane domain	20	42	N/A	INTRINSIC
transmembrane domain	67	89	N/A	INTRINSIC

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.6%
20x: 98.6%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] HMG-CoA reductase is the rate-limiting enzyme for cholesterol synthesis and is regulated via a negative feedback mechanism mediated by sterols and non-sterol metabolites derived from mevalonate, the product of the reaction catalyzed by reductase. Normally in mammalian cells this enzyme is suppressed by cholesterol derived from the internalization and degradation of low density lipoprotein (LDL) via the LDL receptor. Competitive inhibitors of the reductase induce the expression of LDL receptors in the liver, which in turn increases the catabolism of plasma LDL and lowers the plasma concentration of cholesterol, an important determinant of atherosclerosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]
PHENOTYPE: Inactivation of both copies of this gene results in early embryonic lethality. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 93 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930550C14Rik	A	T	9: 53,334,317 (GRCm39)	M187L	possibly damaging	Het
9930012K11Rik	T	C	14: 70,394,686 (GRCm39)	E156G	possibly damaging	Het
Acad9	C	T	3: 36,130,139 (GRCm39)	A194V	probably damaging	Het
Agps	T	A	2: 75,683,128 (GRCm39)	V151E	probably benign	Het
Ak9	A	T	10: 41,283,367 (GRCm39)	I1273L		Het
Akr1c19	T	A	13: 4,296,818 (GRCm39)	L288Q	probably damaging	Het
Carmil1	T	G	13: 24,197,377 (GRCm39)	D1353A	probably damaging	Het
Ccz1	T	A	5: 143,949,897 (GRCm39)	I43F	probably damaging	Het
Cep70	G	A	9: 99,157,638 (GRCm39)	C179Y	probably damaging	Het
Comt	A	T	16: 18,229,440 (GRCm39)	L196H	probably damaging	Het
Cspg5	A	T	9: 110,076,023 (GRCm39)	D253V	probably damaging	Het
Dars2	A	G	1: 160,874,378 (GRCm39)	V437A	possibly damaging	Het
Dcaf5	A	G	12: 80,395,164 (GRCm39)	I335T	probably damaging	Het
Ddn	T	C	15: 98,703,906 (GRCm39)	K462R	possibly damaging	Het
Dscaml1	T	C	9: 45,654,044 (GRCm39)		probably null	Het
Eef1ece2	T	C	16: 20,451,341 (GRCm39)	S325P	probably benign	Het
Ethe1	A	G	7: 24,307,377 (GRCm39)	Y197C	probably damaging	Het
Evc	A	T	5: 37,479,527 (GRCm39)	L269*	probably null	Het
Fam161a	T	C	11: 22,970,001 (GRCm39)	S60P	possibly damaging	Het
Fam20b	A	G	1: 156,509,012 (GRCm39)	V400A	probably benign	Het
Fam53b	T	A	7: 132,361,390 (GRCm39)	S213C	possibly damaging	Het
Flot2	A	G	11: 77,945,612 (GRCm39)	I45V	probably benign	Het
Gemin4	A	C	11: 76,103,579 (GRCm39)	L394R	probably damaging	Het
Glis2	T	G	16: 4,429,182 (GRCm39)	S128R	possibly damaging	Het
Gm3696	A	G	14: 18,435,009 (GRCm39)	Y92H	probably damaging	Het
Gpbp1l1	T	C	4: 116,447,442 (GRCm39)	V374A	probably benign	Het
Grm1	T	C	10: 10,565,440 (GRCm39)	N956S	probably benign	Het
Hbb-bh1	T	C	7: 103,492,238 (GRCm39)	E27G	probably damaging	Het
Hmcn1	A	T	1: 150,458,088 (GRCm39)	C5233S	probably damaging	Het
Hoxb6	A	G	11: 96,183,651 (GRCm39)		probably benign	Het
Igf2	T	C	7: 142,209,555 (GRCm39)	Q35R	possibly damaging	Het
Ighv1-4	C	T	12: 114,450,941 (GRCm39)	V56I	probably benign	Het
Kif13a	C	T	13: 46,905,931 (GRCm39)	V671M	possibly damaging	Het
Lmtk2	C	T	5: 144,111,178 (GRCm39)	Q633*	probably null	Het
Mesd	T	C	7: 83,544,957 (GRCm39)	Y136H	probably damaging	Het
Mga	T	A	2: 119,795,269 (GRCm39)	S2984R	possibly damaging	Het
Mkrn3	T	C	7: 62,068,675 (GRCm39)	N372S	probably benign	Het
Mtpap	A	G	18: 4,387,068 (GRCm39)	I373V	probably benign	Het
Mycbp2	G	A	14: 103,358,027 (GRCm39)	T4589M	probably damaging	Het
Myh2	A	G	11: 67,079,195 (GRCm39)	Q921R	probably benign	Het
Myom1	A	G	17: 71,352,544 (GRCm39)	D324G	possibly damaging	Het
Nat10	T	C	2: 103,584,514 (GRCm39)	K88E	probably benign	Het
Ncapd3	A	G	9: 26,981,254 (GRCm39)	R915G	probably damaging	Het
Nek4	T	C	14: 30,679,249 (GRCm39)	Y190H	probably damaging	Het
Nfasc	A	C	1: 132,529,790 (GRCm39)	Y797D	probably damaging	Het
Ngp	A	G	9: 110,249,978 (GRCm39)	D92G	probably benign	Het
Nos2	A	C	11: 78,820,680 (GRCm39)	H95P	probably damaging	Het
Nr3c1	ACGTC	ACGTCGTC	18: 39,619,513 (GRCm39)		probably benign	Het
Or10ag59	C	T	2: 87,405,863 (GRCm39)	T145I	probably benign	Het
Or5bw2	C	A	7: 6,573,715 (GRCm39)	H242N	probably damaging	Het
Or5p73	T	C	7: 108,064,642 (GRCm39)	I37T	possibly damaging	Het
Or5t18	A	T	2: 86,636,967 (GRCm39)	H125Q	possibly damaging	Het
Otogl	T	A	10: 107,606,471 (GRCm39)	D2154V	probably benign	Het
Pdss1	T	A	2: 22,825,653 (GRCm39)		probably null	Het
Pex5	A	T	6: 124,375,022 (GRCm39)	L609*	probably null	Het
Pglyrp4	C	A	3: 90,640,281 (GRCm39)	A177D	probably damaging	Het
Phactr4	A	G	4: 132,104,489 (GRCm39)		probably null	Het
Phf8-ps	T	C	17: 33,284,501 (GRCm39)	D767G	probably benign	Het
Pik3cd	G	C	4: 149,744,171 (GRCm39)	N193K	probably benign	Het
Prr36	TACCTCTTC	T	8: 4,265,163 (GRCm39)		probably benign	Het
Prss38	A	T	11: 59,266,384 (GRCm39)	W25R	probably benign	Het
Prss8	T	A	7: 127,526,056 (GRCm39)	Q189L	probably damaging	Het
Psd	T	A	19: 46,303,240 (GRCm39)	D713V	probably damaging	Het
Rad51ap2	C	T	12: 11,507,692 (GRCm39)	T538I	probably benign	Het
Rarres1	T	C	3: 67,422,517 (GRCm39)	T78A	probably benign	Het
Rbl2	G	T	8: 91,828,922 (GRCm39)	G651*	probably null	Het
Rev3l	A	T	10: 39,699,601 (GRCm39)	Q1366L	probably damaging	Het
Rundc1	T	C	11: 101,320,413 (GRCm39)	S215P	probably benign	Het
Sanbr	A	T	11: 23,572,479 (GRCm39)	C130S	probably benign	Het
Scarf2	G	A	16: 17,620,837 (GRCm39)	W168*	probably null	Het
Sh2d7	A	G	9: 54,448,186 (GRCm39)	D69G	possibly damaging	Het
Slc26a4	A	T	12: 31,579,527 (GRCm39)	Y578*	probably null	Het
Slc2a9	A	T	5: 38,610,538 (GRCm39)	L87Q	probably damaging	Het
Slc39a10	A	T	1: 46,849,230 (GRCm39)	H795Q	probably damaging	Het
Spata13	C	T	14: 60,993,871 (GRCm39)	R1108W	probably damaging	Het
Sqle	T	C	15: 59,187,901 (GRCm39)	S70P	probably benign	Het
Syncrip	A	T	9: 88,346,716 (GRCm39)	F263I	probably damaging	Het
Synj2	T	C	17: 6,088,220 (GRCm39)	S1424P	possibly damaging	Het
Tax1bp3	A	T	11: 73,071,941 (GRCm39)	T89S	possibly damaging	Het
Tcaim	G	A	9: 122,648,092 (GRCm39)		probably null	Het
Tcp10c	T	C	17: 13,582,438 (GRCm39)	I240T	possibly damaging	Het
Ttll8	G	T	15: 88,801,442 (GRCm39)	N415K	probably benign	Het
Tut7	T	C	13: 59,969,463 (GRCm39)	E144G	probably benign	Het
Ugcg	T	C	4: 59,217,111 (GRCm39)	S212P	possibly damaging	Het
Vmn2r32	T	C	7: 7,482,807 (GRCm39)	K56E	probably benign	Het
Vmn2r55	T	C	7: 12,386,000 (GRCm39)	E660G	probably damaging	Het
Vmn2r7	T	C	3: 64,598,301 (GRCm39)	N752S	probably benign	Het
Vps54	T	A	11: 21,225,005 (GRCm39)	M167K	probably benign	Het
Vwa2	A	C	19: 56,897,791 (GRCm39)	M699L	probably benign	Het
Wdr59	A	G	8: 112,192,494 (GRCm39)	V689A		Het
Whamm	T	G	7: 81,235,995 (GRCm39)	N399K	probably damaging	Het
Zfp760	A	G	17: 21,941,760 (GRCm39)	K312E	probably benign	Het
Zkscan3	C	A	13: 21,578,983 (GRCm39)	V171L	probably benign	Het

Other mutations in Hmgcr

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00557:Hmgcr	APN	13	96,795,786 (GRCm39)	missense	probably benign
IGL01369:Hmgcr	APN	13	96,803,030 (GRCm39)	missense	probably null	1.00
IGL01575:Hmgcr	APN	13	96,793,103 (GRCm39)	missense	possibly damaging	0.56
IGL02183:Hmgcr	APN	13	96,799,635 (GRCm39)	missense	probably damaging	1.00
IGL02515:Hmgcr	APN	13	96,803,020 (GRCm39)	splice site	probably benign
IGL02716:Hmgcr	APN	13	96,796,520 (GRCm39)	critical splice acceptor site	probably null
IGL03278:Hmgcr	APN	13	96,793,270 (GRCm39)	splice site	probably benign
IGL03367:Hmgcr	APN	13	96,802,361 (GRCm39)	missense	probably damaging	0.98
PIT4131001:Hmgcr	UTSW	13	96,795,562 (GRCm39)	missense	probably damaging	1.00
PIT4504001:Hmgcr	UTSW	13	96,799,605 (GRCm39)	missense	possibly damaging	0.95
R0003:Hmgcr	UTSW	13	96,788,653 (GRCm39)	missense	probably damaging	1.00
R0017:Hmgcr	UTSW	13	96,788,597 (GRCm39)	splice site	probably benign
R0017:Hmgcr	UTSW	13	96,788,597 (GRCm39)	splice site	probably benign
R0217:Hmgcr	UTSW	13	96,788,488 (GRCm39)	missense	probably damaging	1.00
R0511:Hmgcr	UTSW	13	96,796,651 (GRCm39)	splice site	probably null
R0707:Hmgcr	UTSW	13	96,787,151 (GRCm39)	unclassified	probably benign
R1301:Hmgcr	UTSW	13	96,795,528 (GRCm39)	missense	probably damaging	0.97
R2203:Hmgcr	UTSW	13	96,793,141 (GRCm39)	missense	probably damaging	1.00
R2204:Hmgcr	UTSW	13	96,793,141 (GRCm39)	missense	probably damaging	1.00
R2433:Hmgcr	UTSW	13	96,802,393 (GRCm39)	missense	probably damaging	1.00
R2938:Hmgcr	UTSW	13	96,799,576 (GRCm39)	missense	probably damaging	0.99
R3159:Hmgcr	UTSW	13	96,802,355 (GRCm39)	missense	probably damaging	1.00
R3737:Hmgcr	UTSW	13	96,787,571 (GRCm39)	missense	probably damaging	1.00
R3752:Hmgcr	UTSW	13	96,799,624 (GRCm39)	missense	probably damaging	1.00
R3837:Hmgcr	UTSW	13	96,795,597 (GRCm39)	missense	probably benign	0.19
R3838:Hmgcr	UTSW	13	96,795,597 (GRCm39)	missense	probably benign	0.19
R3839:Hmgcr	UTSW	13	96,795,597 (GRCm39)	missense	probably benign	0.19
R4034:Hmgcr	UTSW	13	96,787,571 (GRCm39)	missense	probably damaging	1.00
R4035:Hmgcr	UTSW	13	96,787,571 (GRCm39)	missense	probably damaging	1.00
R4210:Hmgcr	UTSW	13	96,796,729 (GRCm39)	missense	probably damaging	1.00
R4783:Hmgcr	UTSW	13	96,802,701 (GRCm39)	missense	probably damaging	1.00
R4820:Hmgcr	UTSW	13	96,796,700 (GRCm39)	missense	probably damaging	1.00
R5090:Hmgcr	UTSW	13	96,787,098 (GRCm39)	missense	probably benign
R5113:Hmgcr	UTSW	13	96,793,240 (GRCm39)	missense	probably benign	0.00
R5209:Hmgcr	UTSW	13	96,803,020 (GRCm39)	splice site	probably benign
R5354:Hmgcr	UTSW	13	96,791,404 (GRCm39)	missense	probably benign	0.26
R5571:Hmgcr	UTSW	13	96,803,171 (GRCm39)	missense	probably benign	0.11
R5804:Hmgcr	UTSW	13	96,802,695 (GRCm39)	missense	probably damaging	0.98
R5886:Hmgcr	UTSW	13	96,796,691 (GRCm39)	missense	probably damaging	1.00
R6340:Hmgcr	UTSW	13	96,802,366 (GRCm39)	missense	probably damaging	1.00
R6638:Hmgcr	UTSW	13	96,795,490 (GRCm39)	missense	probably benign
R6699:Hmgcr	UTSW	13	96,796,717 (GRCm39)	missense	probably damaging	1.00
R7024:Hmgcr	UTSW	13	96,795,418 (GRCm39)	missense	probably benign	0.10
R7061:Hmgcr	UTSW	13	96,802,656 (GRCm39)	missense	possibly damaging	0.64
R7284:Hmgcr	UTSW	13	96,789,173 (GRCm39)	missense	probably damaging	1.00
R7705:Hmgcr	UTSW	13	96,793,231 (GRCm39)	missense	probably benign	0.01
R7709:Hmgcr	UTSW	13	96,799,605 (GRCm39)	missense	possibly damaging	0.95
R9034:Hmgcr	UTSW	13	96,795,885 (GRCm39)	missense	probably damaging	1.00
R9158:Hmgcr	UTSW	13	96,792,170 (GRCm39)	nonsense	probably null
R9253:Hmgcr	UTSW	13	96,796,645 (GRCm39)	missense	probably damaging	1.00
R9474:Hmgcr	UTSW	13	96,796,403 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- AAGCTGTATTTCTCAAGGGAGTG -3'
(R):5'- CACTTTCCAGGATTATGCAGAACG -3'

Sequencing Primer
(F):5'- CCTCTTCAAATTTAGGGCAC -3'
(R):5'- AGTCCTGTACATGAGATGCTGCAC -3'

Posted On

2019-06-26