Mutagenetix > Incidental Mutation

Incidental Mutation 'R7316:Spen'

567953

Institutional Source

Beutler Lab

Gene Symbol

Spen

Ensembl Gene

ENSMUSG00000040761

Gene Name

spen family transcription repressor

Synonyms

Mint

MMRRC Submission

045413-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R7316 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

141195201-141265908 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 141204365 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Serine to Threonine at position 1421 (S1421T)

Ref Sequence

ENSEMBL: ENSMUSP00000101412 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000078886] [ENSMUST00000105786]

AlphaFold

no structure available at present

Predicted Effect

unknown
Transcript: ENSMUST00000078886
AA Change: S1398T

SMART Domains

Protein: ENSMUSP00000077925
Gene: ENSMUSG00000040761
AA Change: S1398T

Domain	Start	End	E-Value	Type
RRM	7	77	7.77e-12	SMART
low complexity region	109	121	N/A	INTRINSIC
low complexity region	235	257	N/A	INTRINSIC
low complexity region	262	311	N/A	INTRINSIC
RRM	338	411	8.6e-5	SMART
RRM	441	511	1.56e-16	SMART
RRM	520	587	1.84e-13	SMART
low complexity region	617	632	N/A	INTRINSIC
low complexity region	669	691	N/A	INTRINSIC
low complexity region	695	720	N/A	INTRINSIC
low complexity region	749	773	N/A	INTRINSIC
coiled coil region	800	825	N/A	INTRINSIC
low complexity region	830	841	N/A	INTRINSIC
internal_repeat_2	844	954	6.27e-5	PROSPERO
coiled coil region	1494	1522	N/A	INTRINSIC
low complexity region	1587	1627	N/A	INTRINSIC
low complexity region	1635	1641	N/A	INTRINSIC
low complexity region	1642	1671	N/A	INTRINSIC
low complexity region	1747	1758	N/A	INTRINSIC
low complexity region	1810	1823	N/A	INTRINSIC
low complexity region	1888	1903	N/A	INTRINSIC
low complexity region	1940	1955	N/A	INTRINSIC
low complexity region	2003	2012	N/A	INTRINSIC
internal_repeat_2	2015	2115	6.27e-5	PROSPERO
low complexity region	2127	2147	N/A	INTRINSIC
low complexity region	2169	2191	N/A	INTRINSIC
low complexity region	2207	2219	N/A	INTRINSIC
low complexity region	2304	2323	N/A	INTRINSIC
low complexity region	2332	2371	N/A	INTRINSIC
low complexity region	2396	2413	N/A	INTRINSIC
low complexity region	2518	2533	N/A	INTRINSIC
low complexity region	2545	2555	N/A	INTRINSIC
low complexity region	2696	2722	N/A	INTRINSIC
low complexity region	2931	2942	N/A	INTRINSIC
low complexity region	2994	3006	N/A	INTRINSIC
low complexity region	3192	3212	N/A	INTRINSIC
low complexity region	3299	3337	N/A	INTRINSIC
Pfam:SPOC	3465	3586	2.7e-29	PFAM

Predicted Effect

unknown
Transcript: ENSMUST00000105786
AA Change: S1421T

SMART Domains

Protein: ENSMUSP00000101412
Gene: ENSMUSG00000040761
AA Change: S1421T

Domain	Start	End	E-Value	Type
RRM	7	77	7.77e-12	SMART
low complexity region	109	121	N/A	INTRINSIC
low complexity region	235	257	N/A	INTRINSIC
low complexity region	262	311	N/A	INTRINSIC
RRM	338	411	8.6e-5	SMART
RRM	441	511	1.56e-16	SMART
RRM	520	587	1.84e-13	SMART
low complexity region	692	714	N/A	INTRINSIC
low complexity region	718	743	N/A	INTRINSIC
low complexity region	772	796	N/A	INTRINSIC
coiled coil region	823	848	N/A	INTRINSIC
low complexity region	853	864	N/A	INTRINSIC
internal_repeat_2	867	977	8.58e-5	PROSPERO
coiled coil region	1517	1545	N/A	INTRINSIC
low complexity region	1610	1650	N/A	INTRINSIC
low complexity region	1658	1664	N/A	INTRINSIC
low complexity region	1665	1694	N/A	INTRINSIC
low complexity region	1770	1781	N/A	INTRINSIC
low complexity region	1833	1846	N/A	INTRINSIC
low complexity region	1911	1926	N/A	INTRINSIC
low complexity region	1963	1978	N/A	INTRINSIC
low complexity region	2026	2035	N/A	INTRINSIC
internal_repeat_2	2038	2138	8.58e-5	PROSPERO
low complexity region	2150	2170	N/A	INTRINSIC
low complexity region	2192	2214	N/A	INTRINSIC
low complexity region	2230	2242	N/A	INTRINSIC
low complexity region	2327	2346	N/A	INTRINSIC
low complexity region	2355	2394	N/A	INTRINSIC
low complexity region	2419	2436	N/A	INTRINSIC
low complexity region	2541	2556	N/A	INTRINSIC
low complexity region	2568	2578	N/A	INTRINSIC
low complexity region	2719	2745	N/A	INTRINSIC
low complexity region	2954	2965	N/A	INTRINSIC
low complexity region	3017	3029	N/A	INTRINSIC
low complexity region	3215	3235	N/A	INTRINSIC
low complexity region	3322	3360	N/A	INTRINSIC
Pfam:SPOC	3488	3609	2.7e-29	PFAM

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.6%
20x: 98.9%

Validation Efficiency

99% (93/94)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a hormone inducible transcriptional repressor. Repression of transcription by this gene product can occur through interactions with other repressors, by the recruitment of proteins involved in histone deacetylation, or through sequestration of transcriptional activators. The product of this gene contains a carboxy-terminal domain that permits binding to other corepressor proteins. This domain also permits interaction with members of the NuRD complex, a nucleosome remodeling protein complex that contains deacetylase activity. In addition, this repressor contains several RNA recognition motifs that confer binding to a steroid receptor RNA coactivator; this binding can modulate the activity of both liganded and nonliganded steroid receptors. [provided by RefSeq, Jul 2008]
PHENOTYPE: Homozygous mutant mice die during late gestation exhibiting morphological abnormalities of the heart, pancreas, and liver. Inactivation of this gene also affects the differentiation of B cells. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 94 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4933434E20Rik	G	A	3: 89,969,020 (GRCm39)	M214I	probably benign	Het
Aadat	G	T	8: 60,979,668 (GRCm39)	G177C	probably damaging	Het
Adam4	T	C	12: 81,466,498 (GRCm39)	I708V	probably benign	Het
Aip	T	C	19: 4,164,793 (GRCm39)	N264S	probably benign	Het
Alox8	A	T	11: 69,077,064 (GRCm39)	D505E	probably benign	Het
Ap3d1	A	T	10: 80,553,693 (GRCm39)	I518N	probably damaging	Het
Arf3	G	A	15: 98,638,874 (GRCm39)	Q128*	probably null	Het
Asph	A	G	4: 9,537,746 (GRCm39)	V344A	probably benign	Het
Birc6	A	T	17: 74,911,489 (GRCm39)	R1500S	probably damaging	Het
Bptf	G	A	11: 106,963,935 (GRCm39)	T1753I	probably damaging	Het
Bptf	A	T	11: 107,001,740 (GRCm39)	C457*	probably null	Het
Brpf3	T	A	17: 29,033,660 (GRCm39)	I568N	probably damaging	Het
Btnl4	G	C	17: 34,688,031 (GRCm39)	N582K	probably benign	Het
Camsap3	C	A	8: 3,654,648 (GRCm39)	P773T	possibly damaging	Het
Capsl	A	T	15: 9,461,888 (GRCm39)	I95L	probably benign	Het
Cd96	T	A	16: 45,890,016 (GRCm39)	M336L	probably benign	Het
Cdan1	A	T	2: 120,558,813 (GRCm39)		probably null	Het
Chd3	A	T	11: 69,236,394 (GRCm39)	L1939Q	probably damaging	Het
Cngb3	A	T	4: 19,425,599 (GRCm39)	Y469F	probably benign	Het
Cp	T	C	3: 20,026,916 (GRCm39)	L448P	probably damaging	Het
Csnk1g2	T	C	10: 80,475,687 (GRCm39)	V418A	possibly damaging	Het
Dag1	A	T	9: 108,086,701 (GRCm39)	S147T	probably benign	Het
Dnajc25	T	A	4: 59,017,693 (GRCm39)	Y117*	probably null	Het
Dpysl2	A	T	14: 67,100,044 (GRCm39)	M64K	possibly damaging	Het
Dus3l	A	G	17: 57,072,551 (GRCm39)	H38R	possibly damaging	Het
Eme2	G	A	17: 25,113,840 (GRCm39)	R62W	probably damaging	Het
Enox1	A	T	14: 77,958,298 (GRCm39)	M616L	probably benign	Het
Epb41l4b	T	A	4: 57,019,867 (GRCm39)	T708S	probably benign	Het
Fat2	A	T	11: 55,176,893 (GRCm39)	I1534N	probably damaging	Het
Fer1l5	A	G	1: 36,457,197 (GRCm39)	T1649A	probably benign	Het
Fmo9	A	G	1: 166,491,215 (GRCm39)	V421A	probably benign	Het
Fsip2	A	C	2: 82,820,035 (GRCm39)	Y5256S	possibly damaging	Het
Gapvd1	G	A	2: 34,594,681 (GRCm39)	L804F	probably damaging	Het
Garin1a	A	G	6: 29,286,101 (GRCm39)	M187V	probably benign	Het
Gen1	A	G	12: 11,291,470 (GRCm39)	V838A	probably benign	Het
Gnptab	A	T	10: 88,236,572 (GRCm39)	N59I	probably damaging	Het
Grm5	A	G	7: 87,624,473 (GRCm39)	T347A	probably benign	Het
Gsdmc3	T	C	15: 63,730,251 (GRCm39)	N438D	possibly damaging	Het
Hmcn1	A	G	1: 150,608,697 (GRCm39)	L1495S	probably damaging	Het
Ift70a1	A	T	2: 75,811,201 (GRCm39)	L294Q	probably damaging	Het
Islr2	T	C	9: 58,105,250 (GRCm39)	E714G	probably damaging	Het
Larp4	A	T	15: 99,898,898 (GRCm39)	M430L	probably benign	Het
Lpcat2b	A	G	5: 107,580,979 (GRCm39)	I103V	not run	Het
Lrguk	C	T	6: 34,080,191 (GRCm39)	S1142F	unknown	Het
Lrit2	G	T	14: 36,790,815 (GRCm39)	V165F	probably damaging	Het
Mast3	T	A	8: 71,232,432 (GRCm39)	I1182F	probably damaging	Het
Mcm6	C	T	1: 128,287,245 (GRCm39)	D20N	probably damaging	Het
Mief1	A	G	15: 80,133,598 (GRCm39)	I218M	probably damaging	Het
Mmp2	A	G	8: 93,567,038 (GRCm39)	T453A	probably benign	Het
Ms4a18	T	A	19: 10,979,360 (GRCm39)	S219C	probably damaging	Het
Myo5c	A	T	9: 75,176,920 (GRCm39)	T622S	probably benign	Het
Neb	A	T	2: 52,161,450 (GRCm39)	M2114K	possibly damaging	Het
Nemp1	T	A	10: 127,525,212 (GRCm39)	L122*	probably null	Het
Nkx2-1	T	C	12: 56,581,583 (GRCm39)	N88S	probably benign	Het
Nr2c2	T	A	6: 92,131,444 (GRCm39)	C202S	probably damaging	Het
Ntsr1	A	C	2: 180,142,545 (GRCm39)	D112A	probably damaging	Het
Or14j3	A	G	17: 37,901,026 (GRCm39)	S73P	probably damaging	Het
Or56a41	A	T	7: 104,740,107 (GRCm39)	H246Q	probably damaging	Het
Or6n2	A	T	1: 173,897,727 (GRCm39)	I288F	probably benign	Het
Osbp2	C	A	11: 3,676,431 (GRCm39)	R73L	probably damaging	Het
Pbp2	T	A	6: 135,286,830 (GRCm39)	E172D	probably damaging	Het
Pcdhgc3	T	C	18: 37,941,501 (GRCm39)	I634T	probably benign	Het
Pcnx1	T	C	12: 82,042,323 (GRCm39)	S38P	probably benign	Het
Phyh	A	T	2: 4,940,855 (GRCm39)	K252*	probably null	Het
Plcb3	C	T	19: 6,943,753 (GRCm39)		probably null	Het
Ppargc1b	T	C	18: 61,440,909 (GRCm39)	S670G	probably damaging	Het
Prorp	A	G	12: 55,351,429 (GRCm39)	D246G	probably damaging	Het
Ptprc	T	C	1: 137,992,509 (GRCm39)	Y1067C	probably damaging	Het
Pwwp2b	A	C	7: 138,836,140 (GRCm39)	E527A	probably benign	Het
Pzp	T	C	6: 128,490,736 (GRCm39)	D409G	probably damaging	Het
Rbfox2	A	T	15: 77,016,929 (GRCm39)	N148K	possibly damaging	Het
Resf1	T	C	6: 149,228,136 (GRCm39)	L394P	probably damaging	Het
Rps24	T	C	14: 24,540,757 (GRCm39)		probably benign	Het
Slc35e4	T	C	11: 3,862,584 (GRCm39)	K202E	probably damaging	Het
Spata7	C	T	12: 98,624,871 (GRCm39)	R258W	probably damaging	Het
Svep1	T	A	4: 58,068,763 (GRCm39)	I3008F	possibly damaging	Het
Tbc1d1	G	T	5: 64,492,620 (GRCm39)		probably null	Het
Thsd4	T	A	9: 59,894,642 (GRCm39)	H772L	probably benign	Het
Tle1	T	C	4: 72,036,529 (GRCm39)	I769V	probably benign	Het
Tm4sf19	T	A	16: 32,226,466 (GRCm39)	S127T	possibly damaging	Het
Tmprss9	C	A	10: 80,730,813 (GRCm39)	R780S	probably benign	Het
Tpp2	T	C	1: 44,009,591 (GRCm39)	V475A	probably benign	Het
Ttn	A	G	2: 76,692,509 (GRCm39)	V471A		Het
Ttn	A	T	2: 76,597,257 (GRCm39)	N19885K	probably damaging	Het
Tub	A	T	7: 108,629,378 (GRCm39)	T478S	possibly damaging	Het
Ube2q2l	T	C	6: 136,378,276 (GRCm39)	K185E	possibly damaging	Het
Vmn2r88	A	T	14: 51,651,712 (GRCm39)	Y342F		Het
Wdr18	C	A	10: 79,801,059 (GRCm39)	D157E	probably benign	Het
Xrra1	A	T	7: 99,525,423 (GRCm39)		probably null	Het
Zc3h4	G	C	7: 16,169,260 (GRCm39)	G1198A	unknown	Het
Zfp568	A	T	7: 29,721,681 (GRCm39)	I209F	possibly damaging	Het
Zfp787	C	A	7: 6,158,523 (GRCm39)		probably benign	Het
Zfp974	A	T	7: 27,609,863 (GRCm39)	Y621N	possibly damaging	Het
Zyg11b	A	G	4: 108,107,699 (GRCm39)	I493T	possibly damaging	Het

Other mutations in Spen

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01133:Spen	APN	4	141,217,212 (GRCm39)	missense	unknown
IGL01357:Spen	APN	4	141,244,424 (GRCm39)	missense	unknown
IGL02184:Spen	APN	4	141,214,917 (GRCm39)	missense	unknown
IGL02226:Spen	APN	4	141,205,457 (GRCm39)	missense	unknown
IGL02321:Spen	APN	4	141,244,441 (GRCm39)	missense	unknown
IGL02350:Spen	APN	4	141,204,890 (GRCm39)	missense	unknown
IGL02357:Spen	APN	4	141,204,890 (GRCm39)	missense	unknown
IGL02627:Spen	APN	4	141,200,326 (GRCm39)	missense	probably damaging	0.99
IGL02683:Spen	APN	4	141,198,956 (GRCm39)	missense	probably benign	0.06
IGL02945:Spen	APN	4	141,221,624 (GRCm39)	missense	unknown
IGL02950:Spen	APN	4	141,196,819 (GRCm39)	missense	probably damaging	1.00
IGL03008:Spen	APN	4	141,203,448 (GRCm39)	missense	possibly damaging	0.70
IGL03019:Spen	APN	4	141,206,227 (GRCm39)	missense	unknown
IGL03038:Spen	APN	4	141,265,550 (GRCm39)	missense	unknown
IGL03334:Spen	APN	4	141,197,280 (GRCm39)	missense	probably damaging	1.00
filtered	UTSW	4	141,204,683 (GRCm39)	missense	unknown
mentholated	UTSW	4	141,196,711 (GRCm39)	missense	possibly damaging	0.78
R0105:Spen	UTSW	4	141,197,121 (GRCm39)	splice site	probably benign
R0268:Spen	UTSW	4	141,204,868 (GRCm39)	missense	unknown
R0359:Spen	UTSW	4	141,244,181 (GRCm39)	missense	unknown
R0394:Spen	UTSW	4	141,201,514 (GRCm39)	missense	probably benign	0.03
R0423:Spen	UTSW	4	141,206,647 (GRCm39)	missense	unknown
R0433:Spen	UTSW	4	141,211,069 (GRCm39)	missense	unknown
R0462:Spen	UTSW	4	141,200,962 (GRCm39)	missense	probably damaging	1.00
R0687:Spen	UTSW	4	141,215,339 (GRCm39)	missense	unknown
R0699:Spen	UTSW	4	141,201,702 (GRCm39)	missense	possibly damaging	0.72
R0865:Spen	UTSW	4	141,199,181 (GRCm39)	missense	probably benign	0.11
R0918:Spen	UTSW	4	141,212,875 (GRCm39)	missense	unknown
R1034:Spen	UTSW	4	141,203,063 (GRCm39)	missense	probably benign	0.33
R1341:Spen	UTSW	4	141,196,711 (GRCm39)	missense	possibly damaging	0.78
R1401:Spen	UTSW	4	141,199,132 (GRCm39)	missense	probably damaging	0.98
R1509:Spen	UTSW	4	141,202,946 (GRCm39)	missense	probably benign	0.00
R1509:Spen	UTSW	4	141,203,011 (GRCm39)	missense	possibly damaging	0.53
R1561:Spen	UTSW	4	141,199,694 (GRCm39)	nonsense	probably null
R1589:Spen	UTSW	4	141,215,335 (GRCm39)	missense	unknown
R1640:Spen	UTSW	4	141,196,254 (GRCm39)	missense	probably damaging	0.98
R1758:Spen	UTSW	4	141,203,686 (GRCm39)	missense	unknown
R1764:Spen	UTSW	4	141,200,261 (GRCm39)	missense	probably damaging	1.00
R1824:Spen	UTSW	4	141,200,096 (GRCm39)	missense	probably damaging	1.00
R1899:Spen	UTSW	4	141,197,654 (GRCm39)	missense	probably benign	0.17
R1916:Spen	UTSW	4	141,199,909 (GRCm39)	missense	probably damaging	1.00
R2011:Spen	UTSW	4	141,200,640 (GRCm39)	missense	probably damaging	1.00
R2295:Spen	UTSW	4	141,204,584 (GRCm39)	missense	unknown
R2379:Spen	UTSW	4	141,244,238 (GRCm39)	missense	unknown
R2404:Spen	UTSW	4	141,205,216 (GRCm39)	missense	unknown
R3719:Spen	UTSW	4	141,244,494 (GRCm39)	missense	unknown
R3889:Spen	UTSW	4	141,205,192 (GRCm39)	missense	unknown
R3945:Spen	UTSW	4	141,204,664 (GRCm39)	missense	unknown
R4227:Spen	UTSW	4	141,249,458 (GRCm39)	missense	unknown
R4326:Spen	UTSW	4	141,204,683 (GRCm39)	missense	unknown
R4382:Spen	UTSW	4	141,200,450 (GRCm39)	missense	possibly damaging	0.88
R4542:Spen	UTSW	4	141,204,097 (GRCm39)	missense	unknown
R4757:Spen	UTSW	4	141,200,390 (GRCm39)	nonsense	probably null
R4771:Spen	UTSW	4	141,199,907 (GRCm39)	missense	probably benign	0.14
R5072:Spen	UTSW	4	141,249,613 (GRCm39)	missense	unknown
R5121:Spen	UTSW	4	141,203,410 (GRCm39)	missense	probably benign	0.00
R5176:Spen	UTSW	4	141,203,587 (GRCm39)	missense	unknown
R5290:Spen	UTSW	4	141,201,127 (GRCm39)	missense	probably damaging	1.00
R5291:Spen	UTSW	4	141,215,390 (GRCm39)	missense	unknown
R5293:Spen	UTSW	4	141,199,717 (GRCm39)	missense	possibly damaging	0.89
R5347:Spen	UTSW	4	141,198,796 (GRCm39)	missense	probably benign	0.26
R5511:Spen	UTSW	4	141,244,149 (GRCm39)	missense	unknown
R5511:Spen	UTSW	4	141,202,375 (GRCm39)	missense	possibly damaging	0.86
R5772:Spen	UTSW	4	141,205,495 (GRCm39)	missense	unknown
R5834:Spen	UTSW	4	141,199,154 (GRCm39)	missense	possibly damaging	0.63
R5858:Spen	UTSW	4	141,201,182 (GRCm39)	missense	probably benign	0.05
R6214:Spen	UTSW	4	141,206,423 (GRCm39)	missense	unknown
R6232:Spen	UTSW	4	141,244,333 (GRCm39)	missense	unknown
R6345:Spen	UTSW	4	141,198,944 (GRCm39)	missense	possibly damaging	0.86
R6419:Spen	UTSW	4	141,203,621 (GRCm39)	missense	unknown
R6455:Spen	UTSW	4	141,202,820 (GRCm39)	missense	probably damaging	0.97
R6979:Spen	UTSW	4	141,205,374 (GRCm39)	missense	unknown
R6994:Spen	UTSW	4	141,220,770 (GRCm39)	missense	unknown
R7018:Spen	UTSW	4	141,220,755 (GRCm39)	missense	unknown
R7040:Spen	UTSW	4	141,221,693 (GRCm39)	missense	unknown
R7127:Spen	UTSW	4	141,203,419 (GRCm39)	missense	possibly damaging	0.53
R7218:Spen	UTSW	4	141,199,961 (GRCm39)	missense	possibly damaging	0.54
R7234:Spen	UTSW	4	141,206,446 (GRCm39)	missense	unknown
R7350:Spen	UTSW	4	141,206,696 (GRCm39)	missense	unknown
R7356:Spen	UTSW	4	141,199,235 (GRCm39)	nonsense	probably null
R7400:Spen	UTSW	4	141,201,052 (GRCm39)	missense	probably damaging	1.00
R7470:Spen	UTSW	4	141,206,605 (GRCm39)	missense	unknown
R7698:Spen	UTSW	4	141,200,156 (GRCm39)	missense	probably damaging	1.00
R7858:Spen	UTSW	4	141,215,442 (GRCm39)	splice site	probably null
R8033:Spen	UTSW	4	141,199,057 (GRCm39)	missense	probably benign	0.03
R8064:Spen	UTSW	4	141,203,011 (GRCm39)	missense	possibly damaging	0.53
R8159:Spen	UTSW	4	141,202,314 (GRCm39)	missense	possibly damaging	0.53
R8187:Spen	UTSW	4	141,200,216 (GRCm39)	missense	possibly damaging	0.93
R8463:Spen	UTSW	4	141,249,590 (GRCm39)	missense	unknown
R8557:Spen	UTSW	4	141,197,681 (GRCm39)	missense	probably benign	0.14
R8558:Spen	UTSW	4	141,197,681 (GRCm39)	missense	probably benign	0.14
R8672:Spen	UTSW	4	141,197,681 (GRCm39)	missense	probably benign	0.14
R8673:Spen	UTSW	4	141,197,681 (GRCm39)	missense	probably benign	0.14
R8674:Spen	UTSW	4	141,197,681 (GRCm39)	missense	probably benign	0.14
R8714:Spen	UTSW	4	141,215,314 (GRCm39)	missense	unknown
R8735:Spen	UTSW	4	141,197,129 (GRCm39)	missense	probably benign	0.32
R8762:Spen	UTSW	4	141,200,261 (GRCm39)	missense	probably damaging	1.00
R8877:Spen	UTSW	4	141,199,137 (GRCm39)	nonsense	probably null
R8878:Spen	UTSW	4	141,204,520 (GRCm39)	missense	unknown
R8937:Spen	UTSW	4	141,201,374 (GRCm39)	missense	probably damaging	1.00
R8939:Spen	UTSW	4	141,202,969 (GRCm39)	missense	possibly damaging	0.72
R8968:Spen	UTSW	4	141,197,701 (GRCm39)	missense	probably benign	0.02
R8971:Spen	UTSW	4	141,201,889 (GRCm39)	missense	possibly damaging	0.53
R9016:Spen	UTSW	4	141,200,938 (GRCm39)	missense	probably damaging	1.00
R9072:Spen	UTSW	4	141,203,702 (GRCm39)	missense	unknown
R9073:Spen	UTSW	4	141,203,702 (GRCm39)	missense	unknown
R9120:Spen	UTSW	4	141,200,233 (GRCm39)	missense
R9136:Spen	UTSW	4	141,249,623 (GRCm39)	missense	unknown
R9138:Spen	UTSW	4	141,196,797 (GRCm39)	missense	probably damaging	1.00
R9150:Spen	UTSW	4	141,244,468 (GRCm39)	missense	unknown
R9225:Spen	UTSW	4	141,202,943 (GRCm39)	missense	possibly damaging	0.53
R9492:Spen	UTSW	4	141,199,098 (GRCm39)	missense	probably benign	0.26
R9537:Spen	UTSW	4	141,244,156 (GRCm39)	small deletion	probably benign
R9537:Spen	UTSW	4	141,199,015 (GRCm39)	missense	probably benign	0.15
R9602:Spen	UTSW	4	141,205,183 (GRCm39)	missense	unknown
R9609:Spen	UTSW	4	141,215,419 (GRCm39)	missense	unknown
R9686:Spen	UTSW	4	141,199,946 (GRCm39)	missense	probably benign	0.27
R9697:Spen	UTSW	4	141,196,275 (GRCm39)	missense	probably damaging	1.00
R9713:Spen	UTSW	4	141,244,331 (GRCm39)	missense	unknown
T0722:Spen	UTSW	4	141,201,664 (GRCm39)	missense	probably benign	0.33
T0975:Spen	UTSW	4	141,201,664 (GRCm39)	missense	probably benign	0.33
Z1088:Spen	UTSW	4	141,205,287 (GRCm39)	missense	unknown
Z1088:Spen	UTSW	4	141,205,288 (GRCm39)	missense	unknown

Predicted Primers

PCR Primer
(F):5'- TTTCTTCACGGGATGATGAGAG -3'
(R):5'- CGGCCAAAATCAAACTGTCTG -3'

Sequencing Primer
(F):5'- AGGGATTTGGCTCTTTCAAGCAAAG -3'
(R):5'- GGCCAAAATCAAACTGTCTGTCTTG -3'

Posted On

2019-06-26