Mutagenetix > Incidental Mutation

Incidental Mutation 'R0639:Akap9'

56895

Institutional Source

Beutler Lab

Gene Symbol

Akap9

Ensembl Gene

ENSMUSG00000040407

Gene Name

A kinase anchor protein 9

Synonyms

mei2-5, repro12, AKAP450, G1-448-15, 5730481H23Rik

MMRRC Submission

038828-MU

Accession Numbers

Essential gene?

Possibly non essential (E-score: 0.351) question?

Stock #

R0639 (G1)

Quality Score

192

Status

Not validated

Chromosome

Chromosomal Location

3977410-4130204 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 4110318 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Leucine to Proline at position 3007 (L3007P)

Ref Sequence

ENSEMBL: ENSMUSP00000046129 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000044492] [ENSMUST00000176863]

AlphaFold

no structure available at present

Predicted Effect

probably damaging
Transcript: ENSMUST00000044492
AA Change: L3007P

PolyPhen 2 Score 0.996 (Sensitivity: 0.55; Specificity: 0.98)

SMART Domains

Protein: ENSMUSP00000046129
Gene: ENSMUSG00000040407
AA Change: L3007P

Domain	Start	End	E-Value	Type
low complexity region	9	18	N/A	INTRINSIC
low complexity region	33	44	N/A	INTRINSIC
low complexity region	98	115	N/A	INTRINSIC
Blast:HPT	126	197	6e-21	BLAST
low complexity region	237	249	N/A	INTRINSIC
low complexity region	297	315	N/A	INTRINSIC
coiled coil region	404	593	N/A	INTRINSIC
coiled coil region	622	756	N/A	INTRINSIC
coiled coil region	777	843	N/A	INTRINSIC
coiled coil region	888	958	N/A	INTRINSIC
low complexity region	982	997	N/A	INTRINSIC
coiled coil region	1037	1065	N/A	INTRINSIC
low complexity region	1233	1246	N/A	INTRINSIC
internal_repeat_2	1247	1312	7.75e-5	PROSPERO
internal_repeat_1	1377	1485	2.63e-5	PROSPERO
coiled coil region	1522	1589	N/A	INTRINSIC
coiled coil region	1789	2107	N/A	INTRINSIC
coiled coil region	2132	2318	N/A	INTRINSIC
internal_repeat_1	2322	2445	2.63e-5	PROSPERO
coiled coil region	2455	2494	N/A	INTRINSIC
low complexity region	2587	2598	N/A	INTRINSIC
low complexity region	2627	2640	N/A	INTRINSIC
internal_repeat_2	2934	2997	7.75e-5	PROSPERO
low complexity region	3000	3016	N/A	INTRINSIC
coiled coil region	3109	3307	N/A	INTRINSIC
coiled coil region	3455	3493	N/A	INTRINSIC
coiled coil region	3521	3556	N/A	INTRINSIC
Pfam:PACT_coil_coil	3576	3657	1.2e-27	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000147629

Predicted Effect

probably benign
Transcript: ENSMUST00000176863

SMART Domains

Protein: ENSMUSP00000135836
Gene: ENSMUSG00000040407

Domain	Start	End	E-Value	Type
coiled coil region	1	90	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000196918

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000197366

Coding Region Coverage

1x: 99.4%
3x: 98.9%
10x: 97.7%
20x: 95.7%

Validation Efficiency

MGI Phenotype

PHENOTYPE: Mice homozygous for a chemically induced allele exhibit male infertily with abnormal spermatogenesis and Sertoli maturation. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 104 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1600014C23Rik	C	T	17: 46,043,999 (GRCm39)	W86*	probably null	Het
Acadl	T	C	1: 66,896,567 (GRCm39)	H75R	probably benign	Het
Adamtsl1	T	C	4: 86,195,380 (GRCm39)	F599S	probably damaging	Het
Adrb3	T	C	8: 27,718,293 (GRCm39)	N52S	probably damaging	Het
Agbl3	T	A	6: 34,776,640 (GRCm39)	L377Q	probably damaging	Het
Amer3	T	A	1: 34,626,902 (GRCm39)	Y380*	probably null	Het
Ankrd13d	A	T	19: 4,323,047 (GRCm39)		probably null	Het
Ap4m1	T	A	5: 138,174,501 (GRCm39)	C235S	probably benign	Het
Arhgap29	T	C	3: 121,801,290 (GRCm39)	F675S	probably damaging	Het
Asah2	C	A	19: 31,986,039 (GRCm39)	V544F	probably damaging	Het
Ash2l	A	G	8: 26,313,319 (GRCm39)	I389T	possibly damaging	Het
Bend5	T	C	4: 111,290,495 (GRCm39)	S164P	probably benign	Het
Cacna1d	A	G	14: 29,893,251 (GRCm39)		probably null	Het
Cdc25b	A	G	2: 131,039,182 (GRCm39)	N516D	probably benign	Het
Cdc27	A	G	11: 104,422,560 (GRCm39)	Y125H	probably damaging	Het
Cdk5r2	C	T	1: 74,894,995 (GRCm39)	L247F	probably damaging	Het
Cenpf	C	A	1: 189,390,259 (GRCm39)	G1191V	probably benign	Het
Cops4	C	T	5: 100,685,326 (GRCm39)	T293I	possibly damaging	Het
Csmd3	A	G	15: 47,777,336 (GRCm39)	L1294P	probably damaging	Het
Dclre1a	T	C	19: 56,526,872 (GRCm39)	Y848C	probably damaging	Het
Disp2	A	T	2: 118,621,325 (GRCm39)	I686F	possibly damaging	Het
Dnah6	T	A	6: 72,999,395 (GRCm39)	Y4012F	probably benign	Het
Dnajc11	C	G	4: 152,054,393 (GRCm39)	R200G	probably damaging	Het
Dnhd1	A	T	7: 105,345,671 (GRCm39)	D2272V	possibly damaging	Het
Elane	A	C	10: 79,722,183 (GRCm39)	R5S	possibly damaging	Het
Entpd7	G	A	19: 43,679,533 (GRCm39)	V29M	probably benign	Het
Fanca	A	G	8: 124,016,098 (GRCm39)		probably null	Het
Fgl1	G	T	8: 41,644,661 (GRCm39)	T281K	probably benign	Het
Flii	T	C	11: 60,613,823 (GRCm39)		probably null	Het
Foxn1	T	C	11: 78,261,970 (GRCm39)	D133G	possibly damaging	Het
Fzd7	T	A	1: 59,523,719 (GRCm39)	M534K	probably damaging	Het
Galnt5	A	G	2: 57,889,407 (GRCm39)	T336A	probably benign	Het
Gli3	G	A	13: 15,899,300 (GRCm39)	D896N	probably damaging	Het
Gsx1	G	T	5: 147,126,756 (GRCm39)	W193L	probably damaging	Het
Gtpbp3	A	T	8: 71,945,379 (GRCm39)	I485F	probably damaging	Het
H2-M11	A	G	17: 36,858,283 (GRCm39)	T26A	probably benign	Het
Igfbp7	T	C	5: 77,499,827 (GRCm39)	D243G	probably damaging	Het
Il31ra	A	T	13: 112,662,377 (GRCm39)	D477E	possibly damaging	Het
Inmt	A	C	6: 55,148,212 (GRCm39)	V139G	probably damaging	Het
Inpp5j	T	A	11: 3,451,147 (GRCm39)	M501L	probably benign	Het
Itsn2	T	C	12: 4,762,556 (GRCm39)	F1579L	probably damaging	Het
Kat2b	C	A	17: 53,874,566 (GRCm39)	A70E	probably benign	Het
Klhl20	T	C	1: 160,921,281 (GRCm39)	E58G	probably damaging	Het
Krt79	A	T	15: 101,839,983 (GRCm39)	Y337*	probably null	Het
Krt81	C	A	15: 101,361,508 (GRCm39)	R24L	possibly damaging	Het
Letm1	T	C	5: 33,926,770 (GRCm39)	I176V	possibly damaging	Het
Lingo3	C	A	10: 80,671,618 (GRCm39)	R104L	probably benign	Het
Lrig3	T	G	10: 125,846,090 (GRCm39)	C840G	probably damaging	Het
Lrrc9	A	G	12: 72,533,062 (GRCm39)	N977S	probably damaging	Het
Lrrk2	T	A	15: 91,657,199 (GRCm39)	M1831K	probably benign	Het
Mn1	A	T	5: 111,567,182 (GRCm39)	D384V	probably damaging	Het
Morc3	C	A	16: 93,650,738 (GRCm39)	H319Q	probably damaging	Het
Morn1	T	C	4: 155,173,960 (GRCm39)	F56L	possibly damaging	Het
Mrpl53	G	T	6: 83,086,392 (GRCm39)	V64L	probably damaging	Het
Myo15a	T	A	11: 60,370,162 (GRCm39)	V974D	probably benign	Het
Neb	A	G	2: 52,146,136 (GRCm39)	V2947A	possibly damaging	Het
Nfasc	A	C	1: 132,531,554 (GRCm39)	N737K	probably damaging	Het
Nlk	T	C	11: 78,463,103 (GRCm39)	D464G	possibly damaging	Het
Nlrc4	C	T	17: 74,733,958 (GRCm39)	R985K	probably benign	Het
Nsun6	T	C	2: 15,001,147 (GRCm39)	K470E	probably benign	Het
Nup85	T	G	11: 115,455,357 (GRCm39)	M1R	probably null	Het
Or8b39	G	A	9: 37,996,666 (GRCm39)	C178Y	probably damaging	Het
Otop1	T	C	5: 38,445,292 (GRCm39)	V150A	possibly damaging	Het
Pclo	C	T	5: 14,731,763 (GRCm39)	R296*	probably null	Het
Pdzd2	A	T	15: 12,458,144 (GRCm39)	C240S	possibly damaging	Het
Plekhg5	A	G	4: 152,198,577 (GRCm39)	T922A	probably benign	Het
Plekhm2	A	C	4: 141,369,381 (GRCm39)	L101R	probably damaging	Het
Plscr3	T	A	11: 69,738,820 (GRCm39)	C161S	probably benign	Het
Prr14l	C	T	5: 32,986,259 (GRCm39)	D1079N	probably benign	Het
Ptpru	A	T	4: 131,498,490 (GRCm39)	V1377E	possibly damaging	Het
Rab37	C	A	11: 115,049,528 (GRCm39)	D112E	probably benign	Het
Raet1e	T	A	10: 22,050,274 (GRCm39)	I19N	probably damaging	Het
Rassf5	T	C	1: 131,172,803 (GRCm39)	Y22C	probably damaging	Het
Rp1	T	C	1: 4,416,721 (GRCm39)	T1464A	probably benign	Het
Safb	T	A	17: 56,908,092 (GRCm39)		probably benign	Het
Scarf2	A	G	16: 17,624,369 (GRCm39)		probably null	Het
Scart2	A	G	7: 139,827,872 (GRCm39)	N27D	probably benign	Het
Sh3d19	T	C	3: 86,014,280 (GRCm39)	S415P	probably benign	Het
Slc26a9	T	A	1: 131,691,542 (GRCm39)	L595Q	probably damaging	Het
Slc4a8	T	C	15: 100,694,431 (GRCm39)	Y470H	probably damaging	Het
Slitrk3	T	C	3: 72,956,982 (GRCm39)	N597D	probably benign	Het
Spata31	T	A	13: 65,070,027 (GRCm39)	V725E	probably benign	Het
Spink12	T	A	18: 44,240,831 (GRCm39)	C72*	probably null	Het
Spink5	T	A	18: 44,146,042 (GRCm39)		probably null	Het
Stk40	C	A	4: 126,012,125 (GRCm39)	S9*	probably null	Het
Sypl1	A	T	12: 33,015,420 (GRCm39)	T40S	probably damaging	Het
Tbc1d8	C	T	1: 39,430,290 (GRCm39)	E438K	probably benign	Het
Tdrd7	A	G	4: 45,989,102 (GRCm39)	T111A	probably benign	Het
Tg	A	T	15: 66,613,333 (GRCm39)		probably null	Het
Tlr5	T	A	1: 182,801,454 (GRCm39)	W253R	probably damaging	Het
Tmprss11c	C	T	5: 86,383,328 (GRCm39)	C353Y	probably damaging	Het
Tnfrsf8	T	A	4: 145,014,597 (GRCm39)	M271L	probably benign	Het
Toe1	T	C	4: 116,663,947 (GRCm39)	N21S	probably benign	Het
Tpp2	T	C	1: 44,014,607 (GRCm39)	F649L	probably benign	Het
Ttll1	G	A	15: 83,386,426 (GRCm39)	Q60*	probably null	Het
Vcp	C	T	4: 42,982,565 (GRCm39)	R709Q	probably benign	Het
Vmn1r119	T	A	7: 20,745,593 (GRCm39)	H263L	possibly damaging	Het
Vmn1r195	C	A	13: 22,463,111 (GRCm39)	Q194K	probably damaging	Het
Vmn1r33	T	C	6: 66,588,783 (GRCm39)	Y257C	probably damaging	Het
Vmn2r15	A	G	5: 109,440,881 (GRCm39)	F326L	probably benign	Het
Wbp11	A	T	6: 136,793,108 (GRCm39)		probably benign	Het
Wwp2	T	G	8: 108,244,578 (GRCm39)	V250G	probably benign	Het
Xpnpep3	T	C	15: 81,315,038 (GRCm39)	V246A	probably benign	Het
Zcchc14	G	A	8: 122,332,188 (GRCm39)	R419*	probably null	Het

Other mutations in Akap9

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00478:Akap9	APN	5	4,096,639 (GRCm39)	missense	probably damaging	0.97
IGL00642:Akap9	APN	5	4,010,842 (GRCm39)	missense	probably damaging	0.99
IGL00786:Akap9	APN	5	4,120,522 (GRCm39)	missense	probably damaging	1.00
IGL00788:Akap9	APN	5	4,110,480 (GRCm39)	missense	probably damaging	1.00
IGL00969:Akap9	APN	5	4,051,550 (GRCm39)	missense	probably benign
IGL01014:Akap9	APN	5	4,018,683 (GRCm39)	missense	probably benign	0.41
IGL01302:Akap9	APN	5	4,020,711 (GRCm39)	missense	probably benign	0.27
IGL01610:Akap9	APN	5	4,082,839 (GRCm39)	missense	possibly damaging	0.95
IGL01620:Akap9	APN	5	4,010,218 (GRCm39)	missense	probably benign	0.11
IGL01862:Akap9	APN	5	4,115,856 (GRCm39)	missense	probably damaging	0.99
IGL01862:Akap9	APN	5	4,001,705 (GRCm39)	missense	probably damaging	0.99
IGL02151:Akap9	APN	5	4,082,728 (GRCm39)	nonsense	probably null
IGL02635:Akap9	APN	5	4,120,500 (GRCm39)	missense	possibly damaging	0.59
IGL02858:Akap9	APN	5	4,119,130 (GRCm39)	missense	possibly damaging	0.88
IGL02967:Akap9	APN	5	4,026,164 (GRCm39)	missense	probably benign	0.07
IGL03064:Akap9	APN	5	4,018,755 (GRCm39)	missense	probably damaging	1.00
IGL03289:Akap9	APN	5	4,127,261 (GRCm39)	missense	probably damaging	1.00
Andy	UTSW	5	4,011,764 (GRCm39)	nonsense	probably null
blimey	UTSW	5	4,120,397 (GRCm39)	nonsense	probably null
hoarder	UTSW	5	4,119,089 (GRCm39)	missense	probably benign	0.00
marinarum	UTSW	5	4,063,875 (GRCm39)	nonsense	probably null
miser	UTSW	5	4,096,064 (GRCm39)	missense	probably benign	0.13
naviculus	UTSW	5	4,010,865 (GRCm39)	missense	probably damaging	0.98
thrifty	UTSW	5	4,026,209 (GRCm39)	missense	probably damaging	0.99
wee_one	UTSW	5	4,093,925 (GRCm39)	missense	probably damaging	1.00
FR4449:Akap9	UTSW	5	4,031,214 (GRCm39)	unclassified	probably benign
PIT1430001:Akap9	UTSW	5	4,079,849 (GRCm39)	missense	probably damaging	1.00
PIT4366001:Akap9	UTSW	5	4,096,221 (GRCm39)	missense	probably benign	0.24
R0088:Akap9	UTSW	5	4,011,946 (GRCm39)	missense	probably benign	0.22
R0309:Akap9	UTSW	5	4,119,038 (GRCm39)	missense	probably benign	0.01
R0387:Akap9	UTSW	5	4,001,678 (GRCm39)	splice site	probably benign
R0440:Akap9	UTSW	5	4,114,569 (GRCm39)	missense	probably damaging	0.99
R0441:Akap9	UTSW	5	4,011,714 (GRCm39)	missense	probably benign	0.15
R0491:Akap9	UTSW	5	4,022,851 (GRCm39)	unclassified	probably benign
R0501:Akap9	UTSW	5	4,020,685 (GRCm39)	missense	probably damaging	1.00
R0507:Akap9	UTSW	5	4,119,043 (GRCm39)	missense	probably benign	0.41
R0544:Akap9	UTSW	5	4,119,185 (GRCm39)	missense	probably benign	0.22
R0581:Akap9	UTSW	5	4,100,620 (GRCm39)	missense	probably benign	0.03
R0611:Akap9	UTSW	5	4,004,870 (GRCm39)	missense	probably benign	0.00
R0620:Akap9	UTSW	5	4,114,136 (GRCm39)	missense	probably damaging	0.98
R0932:Akap9	UTSW	5	4,096,492 (GRCm39)	missense	possibly damaging	0.77
R0944:Akap9	UTSW	5	4,114,742 (GRCm39)	splice site	probably null
R1101:Akap9	UTSW	5	4,096,205 (GRCm39)	missense	probably benign	0.00
R1159:Akap9	UTSW	5	4,010,865 (GRCm39)	missense	probably damaging	0.98
R1170:Akap9	UTSW	5	4,105,671 (GRCm39)	missense	probably benign
R1185:Akap9	UTSW	5	3,998,783 (GRCm39)	missense	probably benign	0.13
R1185:Akap9	UTSW	5	3,998,783 (GRCm39)	missense	probably benign	0.13
R1185:Akap9	UTSW	5	3,998,783 (GRCm39)	missense	probably benign	0.13
R1453:Akap9	UTSW	5	4,025,614 (GRCm39)	splice site	probably null
R1551:Akap9	UTSW	5	4,119,174 (GRCm39)	missense	probably benign	0.02
R1608:Akap9	UTSW	5	4,011,783 (GRCm39)	missense	probably damaging	1.00
R1652:Akap9	UTSW	5	4,127,210 (GRCm39)	missense	probably damaging	1.00
R1659:Akap9	UTSW	5	4,114,633 (GRCm39)	missense	probably damaging	1.00
R1713:Akap9	UTSW	5	4,089,345 (GRCm39)	critical splice donor site	probably null
R1719:Akap9	UTSW	5	4,007,645 (GRCm39)	nonsense	probably null
R1720:Akap9	UTSW	5	4,022,791 (GRCm39)	missense	possibly damaging	0.63
R1757:Akap9	UTSW	5	4,051,667 (GRCm39)	missense	probably benign	0.41
R1872:Akap9	UTSW	5	4,051,406 (GRCm39)	missense	probably damaging	1.00
R1876:Akap9	UTSW	5	4,011,809 (GRCm39)	missense	probably benign	0.28
R1881:Akap9	UTSW	5	4,100,173 (GRCm39)	missense	probably benign
R1950:Akap9	UTSW	5	4,010,677 (GRCm39)	missense	probably damaging	1.00
R1980:Akap9	UTSW	5	4,022,771 (GRCm39)	missense	probably damaging	0.99
R1993:Akap9	UTSW	5	4,088,520 (GRCm39)	splice site	probably null
R2008:Akap9	UTSW	5	4,010,131 (GRCm39)	missense	possibly damaging	0.47
R2020:Akap9	UTSW	5	4,011,967 (GRCm39)	missense	probably damaging	1.00
R2051:Akap9	UTSW	5	4,025,685 (GRCm39)	nonsense	probably null
R2061:Akap9	UTSW	5	4,011,010 (GRCm39)	missense	probably damaging	1.00
R2109:Akap9	UTSW	5	4,094,847 (GRCm39)	missense	possibly damaging	0.47
R2135:Akap9	UTSW	5	4,114,509 (GRCm39)	missense	probably damaging	1.00
R2225:Akap9	UTSW	5	4,127,271 (GRCm39)	missense	probably damaging	0.96
R2232:Akap9	UTSW	5	4,096,603 (GRCm39)	missense	probably damaging	1.00
R2424:Akap9	UTSW	5	4,115,279 (GRCm39)	missense	probably damaging	0.97
R2483:Akap9	UTSW	5	4,026,235 (GRCm39)	missense	possibly damaging	0.65
R2879:Akap9	UTSW	5	4,026,353 (GRCm39)	intron	probably benign
R3622:Akap9	UTSW	5	4,026,235 (GRCm39)	missense	possibly damaging	0.65
R3623:Akap9	UTSW	5	4,026,235 (GRCm39)	missense	possibly damaging	0.65
R3624:Akap9	UTSW	5	4,026,235 (GRCm39)	missense	possibly damaging	0.65
R3722:Akap9	UTSW	5	4,120,351 (GRCm39)	missense	probably damaging	1.00
R3806:Akap9	UTSW	5	4,004,410 (GRCm39)	missense	probably benign	0.00
R3919:Akap9	UTSW	5	4,011,764 (GRCm39)	nonsense	probably null
R4023:Akap9	UTSW	5	4,042,077 (GRCm39)	missense	possibly damaging	0.66
R4093:Akap9	UTSW	5	4,093,996 (GRCm39)	missense	probably damaging	0.99
R4434:Akap9	UTSW	5	4,082,708 (GRCm39)	missense	probably damaging	0.99
R4529:Akap9	UTSW	5	4,093,948 (GRCm39)	missense	probably damaging	1.00
R4530:Akap9	UTSW	5	4,093,948 (GRCm39)	missense	probably damaging	1.00
R4532:Akap9	UTSW	5	4,093,948 (GRCm39)	missense	probably damaging	1.00
R4533:Akap9	UTSW	5	4,093,948 (GRCm39)	missense	probably damaging	1.00
R4585:Akap9	UTSW	5	4,026,151 (GRCm39)	missense	probably benign	0.00
R4586:Akap9	UTSW	5	4,026,151 (GRCm39)	missense	probably benign	0.00
R4655:Akap9	UTSW	5	4,096,403 (GRCm39)	missense	probably benign	0.14
R4676:Akap9	UTSW	5	4,114,515 (GRCm39)	nonsense	probably null
R4676:Akap9	UTSW	5	4,082,774 (GRCm39)	missense	probably damaging	1.00
R4724:Akap9	UTSW	5	4,105,339 (GRCm39)	missense	probably benign
R4731:Akap9	UTSW	5	4,012,266 (GRCm39)	missense	possibly damaging	0.54
R4732:Akap9	UTSW	5	4,063,901 (GRCm39)	missense	probably damaging	0.98
R4733:Akap9	UTSW	5	4,063,901 (GRCm39)	missense	probably damaging	0.98
R4743:Akap9	UTSW	5	4,011,013 (GRCm39)	missense	probably damaging	1.00
R4749:Akap9	UTSW	5	4,018,737 (GRCm39)	missense	probably benign	0.41
R4756:Akap9	UTSW	5	4,051,418 (GRCm39)	missense	probably damaging	0.99
R4757:Akap9	UTSW	5	4,058,382 (GRCm39)	missense	probably damaging	1.00
R4860:Akap9	UTSW	5	4,084,916 (GRCm39)	intron	probably benign
R4937:Akap9	UTSW	5	4,100,145 (GRCm39)	splice site	probably null
R4960:Akap9	UTSW	5	4,007,664 (GRCm39)	missense	probably benign	0.15
R4974:Akap9	UTSW	5	4,011,466 (GRCm39)	missense	possibly damaging	0.81
R5101:Akap9	UTSW	5	4,051,748 (GRCm39)	missense	probably damaging	0.96
R5160:Akap9	UTSW	5	4,080,007 (GRCm39)	missense	probably damaging	1.00
R5200:Akap9	UTSW	5	4,010,734 (GRCm39)	missense	probably benign	0.00
R5245:Akap9	UTSW	5	4,026,209 (GRCm39)	missense	probably damaging	0.99
R5293:Akap9	UTSW	5	3,998,687 (GRCm39)	missense	probably damaging	0.99
R5408:Akap9	UTSW	5	4,108,458 (GRCm39)	missense	possibly damaging	0.84
R5507:Akap9	UTSW	5	4,018,683 (GRCm39)	missense	probably benign	0.41
R5517:Akap9	UTSW	5	4,051,665 (GRCm39)	missense	possibly damaging	0.76
R5579:Akap9	UTSW	5	4,114,714 (GRCm39)	missense	possibly damaging	0.93
R5619:Akap9	UTSW	5	4,004,760 (GRCm39)	intron	probably benign
R5645:Akap9	UTSW	5	4,100,590 (GRCm39)	missense	probably benign	0.09
R5669:Akap9	UTSW	5	4,100,540 (GRCm39)	nonsense	probably null
R5686:Akap9	UTSW	5	4,021,926 (GRCm39)	missense	probably benign	0.00
R5697:Akap9	UTSW	5	4,010,170 (GRCm39)	missense	possibly damaging	0.92
R5821:Akap9	UTSW	5	4,096,064 (GRCm39)	missense	probably benign	0.13
R5875:Akap9	UTSW	5	4,127,285 (GRCm39)	missense	probably benign	0.01
R5897:Akap9	UTSW	5	4,127,904 (GRCm39)	missense	probably benign	0.23
R5999:Akap9	UTSW	5	4,093,925 (GRCm39)	missense	probably damaging	1.00
R6025:Akap9	UTSW	5	4,082,801 (GRCm39)	missense	probably damaging	1.00
R6078:Akap9	UTSW	5	4,117,924 (GRCm39)	critical splice donor site	probably null
R6138:Akap9	UTSW	5	4,117,924 (GRCm39)	critical splice donor site	probably null
R6225:Akap9	UTSW	5	4,012,105 (GRCm39)	missense	probably damaging	1.00
R6243:Akap9	UTSW	5	4,115,000 (GRCm39)	splice site	probably null
R6326:Akap9	UTSW	5	4,012,061 (GRCm39)	missense	probably damaging	1.00
R6564:Akap9	UTSW	5	4,078,491 (GRCm39)	missense	probably damaging	0.98
R6617:Akap9	UTSW	5	4,018,745 (GRCm39)	missense	probably benign	0.04
R6625:Akap9	UTSW	5	4,018,745 (GRCm39)	missense	probably benign	0.04
R6632:Akap9	UTSW	5	4,063,842 (GRCm39)	splice site	probably null
R6677:Akap9	UTSW	5	4,079,869 (GRCm39)	missense	probably benign	0.21
R6717:Akap9	UTSW	5	4,114,086 (GRCm39)	missense	probably damaging	1.00
R6893:Akap9	UTSW	5	4,011,709 (GRCm39)	missense	probably benign	0.32
R6915:Akap9	UTSW	5	4,010,551 (GRCm39)	missense	probably benign	0.03
R6938:Akap9	UTSW	5	4,096,628 (GRCm39)	missense	possibly damaging	0.91
R6972:Akap9	UTSW	5	4,096,699 (GRCm39)	missense	possibly damaging	0.62
R6973:Akap9	UTSW	5	4,096,699 (GRCm39)	missense	possibly damaging	0.62
R6993:Akap9	UTSW	5	4,115,866 (GRCm39)	missense	possibly damaging	0.65
R7032:Akap9	UTSW	5	4,004,896 (GRCm39)	missense	probably benign
R7164:Akap9	UTSW	5	4,110,364 (GRCm39)	missense	probably damaging	0.96
R7170:Akap9	UTSW	5	4,018,745 (GRCm39)	missense	probably benign	0.04
R7192:Akap9	UTSW	5	4,055,723 (GRCm39)	splice site	probably null
R7284:Akap9	UTSW	5	4,006,246 (GRCm39)	missense	probably damaging	1.00
R7299:Akap9	UTSW	5	4,082,696 (GRCm39)	missense	probably damaging	1.00
R7313:Akap9	UTSW	5	4,054,933 (GRCm39)	missense	probably damaging	1.00
R7326:Akap9	UTSW	5	4,095,930 (GRCm39)	missense	possibly damaging	0.47
R7343:Akap9	UTSW	5	4,096,364 (GRCm39)	missense	probably damaging	0.99
R7455:Akap9	UTSW	5	4,022,792 (GRCm39)	missense	probably benign	0.03
R7482:Akap9	UTSW	5	4,018,745 (GRCm39)	missense	probably benign	0.04
R7489:Akap9	UTSW	5	4,054,933 (GRCm39)	missense	probably damaging	1.00
R7525:Akap9	UTSW	5	4,018,745 (GRCm39)	missense	probably benign	0.04
R7528:Akap9	UTSW	5	4,018,745 (GRCm39)	missense	probably benign	0.04
R7576:Akap9	UTSW	5	4,018,745 (GRCm39)	missense	probably benign	0.04
R7577:Akap9	UTSW	5	4,018,745 (GRCm39)	missense	probably benign	0.04
R7578:Akap9	UTSW	5	4,018,745 (GRCm39)	missense	probably benign	0.04
R7610:Akap9	UTSW	5	4,007,677 (GRCm39)	missense	possibly damaging	0.95
R7658:Akap9	UTSW	5	4,018,745 (GRCm39)	missense	probably benign	0.04
R7754:Akap9	UTSW	5	4,096,736 (GRCm39)	missense	probably benign	0.03
R7818:Akap9	UTSW	5	4,063,875 (GRCm39)	nonsense	probably null
R7979:Akap9	UTSW	5	4,100,381 (GRCm39)	missense	probably benign
R7991:Akap9	UTSW	5	4,114,949 (GRCm39)	splice site	probably null
R8036:Akap9	UTSW	5	4,120,397 (GRCm39)	nonsense	probably null
R8054:Akap9	UTSW	5	4,088,707 (GRCm39)	critical splice donor site	probably null
R8116:Akap9	UTSW	5	4,111,183 (GRCm39)	missense	probably benign	0.04
R8150:Akap9	UTSW	5	4,011,982 (GRCm39)	missense	probably damaging	1.00
R8234:Akap9	UTSW	5	4,094,845 (GRCm39)	missense	probably benign	0.18
R8348:Akap9	UTSW	5	3,998,897 (GRCm39)	critical splice donor site	probably null
R8365:Akap9	UTSW	5	4,018,745 (GRCm39)	missense	probably benign	0.04
R8366:Akap9	UTSW	5	4,018,745 (GRCm39)	missense	probably benign	0.04
R8448:Akap9	UTSW	5	3,998,897 (GRCm39)	critical splice donor site	probably null
R8466:Akap9	UTSW	5	4,088,659 (GRCm39)	missense	probably damaging	1.00
R8772:Akap9	UTSW	5	4,096,255 (GRCm39)	missense	probably damaging	1.00
R8881:Akap9	UTSW	5	4,011,279 (GRCm39)	missense
R8937:Akap9	UTSW	5	4,094,048 (GRCm39)	missense	possibly damaging	0.78
R8956:Akap9	UTSW	5	3,998,805 (GRCm39)	missense	possibly damaging	0.79
R9000:Akap9	UTSW	5	4,105,650 (GRCm39)	missense	probably benign
R9049:Akap9	UTSW	5	4,114,597 (GRCm39)	missense
R9074:Akap9	UTSW	5	4,127,959 (GRCm39)	missense	probably benign	0.40
R9124:Akap9	UTSW	5	4,111,284 (GRCm39)	missense	probably damaging	0.99
R9129:Akap9	UTSW	5	4,119,089 (GRCm39)	missense	probably benign	0.00
R9371:Akap9	UTSW	5	4,011,852 (GRCm39)	missense	possibly damaging	0.83
R9424:Akap9	UTSW	5	4,012,224 (GRCm39)	nonsense	probably null
R9424:Akap9	UTSW	5	4,012,223 (GRCm39)	nonsense	probably null
R9509:Akap9	UTSW	5	4,096,349 (GRCm39)	missense	probably benign
R9515:Akap9	UTSW	5	4,105,709 (GRCm39)	missense	probably damaging	1.00
R9567:Akap9	UTSW	5	4,127,311 (GRCm39)	missense	possibly damaging	0.89
R9587:Akap9	UTSW	5	4,119,149 (GRCm39)	missense	probably damaging	1.00
R9619:Akap9	UTSW	5	4,094,833 (GRCm39)	missense	probably damaging	1.00
R9635:Akap9	UTSW	5	4,100,545 (GRCm39)	missense	probably benign	0.20
R9680:Akap9	UTSW	5	4,011,587 (GRCm39)	missense	probably benign	0.03
R9691:Akap9	UTSW	5	4,010,491 (GRCm39)	missense	probably damaging	1.00
R9726:Akap9	UTSW	5	4,053,757 (GRCm39)	missense	probably benign	0.39
U15987:Akap9	UTSW	5	4,117,924 (GRCm39)	critical splice donor site	probably null
X0026:Akap9	UTSW	5	4,064,039 (GRCm39)	missense	probably damaging	1.00
X0057:Akap9	UTSW	5	4,025,598 (GRCm39)	critical splice acceptor site	probably null
Z1176:Akap9	UTSW	5	4,012,251 (GRCm39)	missense	probably damaging	0.96
Z1177:Akap9	UTSW	5	4,096,189 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- AGCATTTGCCCCACTCTGAGAAAG -3'
(R):5'- GCAGAGGTTCTGCCAAAACAATCAC -3'

Sequencing Primer
(F):5'- ACACTGTAGATTATCAGTCTAGGGG -3'
(R):5'- CGCTTTCTGCATAATGGTATGACAC -3'

Posted On

2013-07-11