Incidental Mutation 'R7335:Slco3a1'
ID 569437
Institutional Source Beutler Lab
Gene Symbol Slco3a1
Ensembl Gene ENSMUSG00000025790
Gene Name solute carrier organic anion transporter family, member 3a1
Synonyms OATP-D, Slc21a11, 5830414C08Rik, Anr1, MJAM
MMRRC Submission 045372-MU
Accession Numbers
Essential gene? Probably non essential (E-score: 0.112) question?
Stock # R7335 (G1)
Quality Score 225.009
Status Validated
Chromosome 7
Chromosomal Location 73925167-74204528 bp(-) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) A to G at 73934090 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Isoleucine to Threonine at position 694 (I694T)
Ref Sequence ENSEMBL: ENSMUSP00000026897 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000026897] [ENSMUST00000098371]
AlphaFold Q8R3L5
Predicted Effect probably damaging
Transcript: ENSMUST00000026897
AA Change: I694T

PolyPhen 2 Score 0.991 (Sensitivity: 0.71; Specificity: 0.97)
SMART Domains Protein: ENSMUSP00000026897
Gene: ENSMUSG00000025790
AA Change: I694T

DomainStartEndE-ValueType
low complexity region 7 16 N/A INTRINSIC
Pfam:MFS_1 44 455 1.2e-27 PFAM
KAZAL 474 509 2.77e-1 SMART
low complexity region 574 594 N/A INTRINSIC
transmembrane domain 626 648 N/A INTRINSIC
Predicted Effect probably benign
Transcript: ENSMUST00000098371
SMART Domains Protein: ENSMUSP00000095973
Gene: ENSMUSG00000025790

DomainStartEndE-ValueType
low complexity region 7 16 N/A INTRINSIC
Pfam:MFS_1 44 456 1.2e-27 PFAM
KAZAL 474 509 2.77e-1 SMART
low complexity region 574 594 N/A INTRINSIC
transmembrane domain 626 648 N/A INTRINSIC
Meta Mutation Damage Score 0.1129 question?
Coding Region Coverage
  • 1x: 100.0%
  • 3x: 100.0%
  • 10x: 99.7%
  • 20x: 99.1%
Validation Efficiency 100% (56/56)
Allele List at MGI
Other mutations in this stock
Total: 57 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Adamts2 A G 11: 50,493,093 (GRCm39) S35G probably benign Het
Adcy6 G T 15: 98,501,757 (GRCm39) Q286K probably benign Het
Agrn A G 4: 156,260,989 (GRCm39) S676P probably damaging Het
Arpp21 A T 9: 112,005,319 (GRCm39) probably null Het
Bmal2 A G 6: 146,711,217 (GRCm39) M74V probably benign Het
C1rb A T 6: 124,552,238 (GRCm39) I319F possibly damaging Het
C2cd3 T C 7: 100,071,810 (GRCm39) V629A Het
Cacna1i T G 15: 80,259,776 (GRCm39) F1287C probably damaging Het
Catspere2 A G 1: 177,926,074 (GRCm39) I247V probably benign Het
Cd101 C G 3: 100,926,045 (GRCm39) A229P probably benign Het
Cdh23 T C 10: 60,140,895 (GRCm39) D3151G probably damaging Het
Cenpc1 C A 5: 86,182,212 (GRCm39) K557N possibly damaging Het
Col6a4 G A 9: 105,954,091 (GRCm39) T416M possibly damaging Het
Cyp2c67 A T 19: 39,628,451 (GRCm39) C164* probably null Het
Dop1b A G 16: 93,544,396 (GRCm39) E179G probably benign Het
Duoxa2 A T 2: 122,131,821 (GRCm39) Y170F possibly damaging Het
Fras1 A G 5: 96,884,829 (GRCm39) K2569R possibly damaging Het
Fsip2 C A 2: 82,813,462 (GRCm39) H3260Q probably benign Het
Gm3402 T C 5: 146,452,043 (GRCm39) I121T probably benign Het
Herc3 T A 6: 58,853,773 (GRCm39) H606Q possibly damaging Het
Hmcn2 T C 2: 31,282,169 (GRCm39) V1927A possibly damaging Het
Hnf4g A G 3: 3,717,924 (GRCm39) H347R possibly damaging Het
Hs3st3a1 A G 11: 64,411,163 (GRCm39) T234A probably benign Het
Hyal1 G T 9: 107,456,359 (GRCm39) V379L probably benign Het
Ighv1-19 A G 12: 114,672,571 (GRCm39) probably benign Het
Iqcc T A 4: 129,510,501 (GRCm39) Q338L not run Het
Kdm5b T C 1: 134,488,177 (GRCm39) V34A probably damaging Het
Lgals4 T C 7: 28,540,571 (GRCm39) F225L probably benign Het
Lmtk3 C T 7: 45,444,581 (GRCm39) T1088I unknown Het
Magel2 A T 7: 62,030,524 (GRCm39) S1143C unknown Het
Mapk4 A T 18: 74,070,338 (GRCm39) L185Q possibly damaging Het
Mtcl2 T C 2: 156,872,925 (GRCm39) Q862R possibly damaging Het
Ncam1 A G 9: 49,418,211 (GRCm39) S1030P Het
Nynrin A T 14: 56,101,371 (GRCm39) T347S probably benign Het
Pak5 T C 2: 135,940,219 (GRCm39) T532A probably damaging Het
Pcdhb15 A G 18: 37,607,389 (GRCm39) E207G probably damaging Het
Pde6h T C 6: 136,940,211 (GRCm39) W66R probably damaging Het
Pmaip1 T C 18: 66,591,885 (GRCm39) probably null Het
Prkd3 A G 17: 79,261,995 (GRCm39) F774S probably damaging Het
Prox1 T A 1: 189,894,042 (GRCm39) R134S possibly damaging Het
Ptpn22 T A 3: 103,793,335 (GRCm39) H495Q probably damaging Het
Ptprj G T 2: 90,271,126 (GRCm39) Q1201K probably benign Het
Rp1l1 A G 14: 64,269,447 (GRCm39) S1678G probably benign Het
Sez6l G T 5: 112,724,678 (GRCm39) probably null Het
Sgcg T A 14: 61,477,816 (GRCm39) Y91F probably damaging Het
Skint2 C T 4: 112,481,415 (GRCm39) L93F probably damaging Het
Slc22a22 A T 15: 57,126,771 (GRCm39) D99E probably benign Het
Smurf2 A T 11: 106,736,911 (GRCm39) I305N possibly damaging Het
Stac3 T A 10: 127,340,769 (GRCm39) V178D probably benign Het
Suclg2 G A 6: 95,543,444 (GRCm39) A329V probably damaging Het
Suclg2 T A 6: 95,543,441 (GRCm39) N330I probably damaging Het
Tas2r118 C T 6: 23,969,749 (GRCm39) C104Y probably damaging Het
Tom1l2 T G 11: 60,135,991 (GRCm39) E327D probably benign Het
Trit1 T C 4: 122,910,572 (GRCm39) M57T possibly damaging Het
Vav1 G A 17: 57,603,720 (GRCm39) S134N probably benign Het
Vmn2r82 T C 10: 79,214,722 (GRCm39) L235P probably damaging Het
Zfp735 T A 11: 73,602,379 (GRCm39) V441E possibly damaging Het
Other mutations in Slco3a1
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL00897:Slco3a1 APN 7 74,153,931 (GRCm39) missense probably damaging 1.00
IGL01124:Slco3a1 APN 7 73,934,295 (GRCm39) missense probably damaging 1.00
IGL01583:Slco3a1 APN 7 73,934,198 (GRCm39) missense probably benign 0.01
IGL01929:Slco3a1 APN 7 73,968,353 (GRCm39) splice site probably benign
IGL01991:Slco3a1 APN 7 73,934,144 (GRCm39) missense possibly damaging 0.84
IGL02380:Slco3a1 APN 7 74,204,238 (GRCm39) missense probably damaging 1.00
IGL03269:Slco3a1 APN 7 73,968,280 (GRCm39) missense possibly damaging 0.58
R0052:Slco3a1 UTSW 7 74,154,074 (GRCm39) missense probably benign 0.00
R0052:Slco3a1 UTSW 7 74,154,074 (GRCm39) missense probably benign 0.00
R0317:Slco3a1 UTSW 7 74,154,174 (GRCm39) missense probably damaging 1.00
R0545:Slco3a1 UTSW 7 73,970,301 (GRCm39) nonsense probably null
R0613:Slco3a1 UTSW 7 73,996,382 (GRCm39) unclassified probably benign
R1488:Slco3a1 UTSW 7 73,996,449 (GRCm39) missense possibly damaging 0.94
R1506:Slco3a1 UTSW 7 74,009,683 (GRCm39) splice site probably null
R1571:Slco3a1 UTSW 7 74,154,128 (GRCm39) missense possibly damaging 0.92
R1912:Slco3a1 UTSW 7 74,154,359 (GRCm39) missense probably damaging 1.00
R2011:Slco3a1 UTSW 7 73,996,419 (GRCm39) missense probably benign 0.08
R2382:Slco3a1 UTSW 7 73,996,524 (GRCm39) missense probably benign 0.00
R3735:Slco3a1 UTSW 7 74,154,245 (GRCm39) missense probably damaging 1.00
R3894:Slco3a1 UTSW 7 73,934,361 (GRCm39) missense probably damaging 1.00
R4151:Slco3a1 UTSW 7 74,009,586 (GRCm39) missense probably damaging 1.00
R4175:Slco3a1 UTSW 7 73,968,302 (GRCm39) missense probably damaging 0.97
R4303:Slco3a1 UTSW 7 74,204,276 (GRCm39) missense probably benign 0.03
R4462:Slco3a1 UTSW 7 74,204,311 (GRCm39) missense probably benign 0.18
R4702:Slco3a1 UTSW 7 73,970,315 (GRCm39) missense probably damaging 0.98
R4896:Slco3a1 UTSW 7 73,970,304 (GRCm39) missense probably null 1.00
R5419:Slco3a1 UTSW 7 73,934,363 (GRCm39) missense possibly damaging 0.77
R5561:Slco3a1 UTSW 7 73,968,247 (GRCm39) missense possibly damaging 0.67
R5597:Slco3a1 UTSW 7 73,934,210 (GRCm39) missense probably benign 0.31
R5698:Slco3a1 UTSW 7 73,996,566 (GRCm39) missense probably damaging 1.00
R6086:Slco3a1 UTSW 7 73,968,338 (GRCm39) missense possibly damaging 0.64
R6117:Slco3a1 UTSW 7 73,968,254 (GRCm39) missense probably benign 0.02
R6118:Slco3a1 UTSW 7 73,968,254 (GRCm39) missense probably benign 0.02
R6123:Slco3a1 UTSW 7 73,968,254 (GRCm39) missense probably benign 0.02
R6124:Slco3a1 UTSW 7 73,968,254 (GRCm39) missense probably benign 0.02
R6125:Slco3a1 UTSW 7 73,968,254 (GRCm39) missense probably benign 0.02
R7147:Slco3a1 UTSW 7 74,154,042 (GRCm39) missense probably damaging 1.00
R7332:Slco3a1 UTSW 7 73,968,232 (GRCm39) missense possibly damaging 0.95
R7646:Slco3a1 UTSW 7 74,154,344 (GRCm39) missense probably damaging 1.00
R7798:Slco3a1 UTSW 7 73,968,344 (GRCm39) missense probably benign 0.00
R8024:Slco3a1 UTSW 7 74,204,218 (GRCm39) missense probably benign 0.24
R8128:Slco3a1 UTSW 7 73,934,049 (GRCm39) missense probably damaging 1.00
R8184:Slco3a1 UTSW 7 74,009,577 (GRCm39) missense probably benign 0.01
R8192:Slco3a1 UTSW 7 73,970,338 (GRCm39) missense probably benign 0.13
R8279:Slco3a1 UTSW 7 73,934,144 (GRCm39) missense possibly damaging 0.84
R8511:Slco3a1 UTSW 7 73,952,990 (GRCm39) missense probably benign 0.33
R8732:Slco3a1 UTSW 7 73,934,054 (GRCm39) missense possibly damaging 0.47
R8933:Slco3a1 UTSW 7 73,934,248 (GRCm39) nonsense probably null
R8987:Slco3a1 UTSW 7 73,970,324 (GRCm39) missense possibly damaging 0.92
R9138:Slco3a1 UTSW 7 74,009,664 (GRCm39) missense probably damaging 1.00
R9177:Slco3a1 UTSW 7 73,952,946 (GRCm39) missense probably benign 0.40
R9268:Slco3a1 UTSW 7 73,952,946 (GRCm39) missense probably benign 0.40
R9310:Slco3a1 UTSW 7 74,204,236 (GRCm39) missense probably damaging 0.99
R9342:Slco3a1 UTSW 7 74,154,037 (GRCm39) missense probably damaging 1.00
R9347:Slco3a1 UTSW 7 73,934,153 (GRCm39) missense possibly damaging 0.89
R9422:Slco3a1 UTSW 7 73,946,996 (GRCm39) missense probably damaging 1.00
R9556:Slco3a1 UTSW 7 74,201,905 (GRCm39) missense probably benign 0.00
R9560:Slco3a1 UTSW 7 74,153,931 (GRCm39) missense probably damaging 1.00
R9709:Slco3a1 UTSW 7 73,952,957 (GRCm39) missense possibly damaging 0.62
X0017:Slco3a1 UTSW 7 73,934,108 (GRCm39) missense probably benign 0.03
Z1176:Slco3a1 UTSW 7 73,925,762 (GRCm39) nonsense probably null
Predicted Primers PCR Primer
(F):5'- CTGAATCCTGACTGGCTCTG -3'
(R):5'- ATTGCGCTCAAGTCCTTTGC -3'

Sequencing Primer
(F):5'- CTGGCTCTGAGTTTTAGAAAAAGGAC -3'
(R):5'- GCTCAAGTCCTTTGCCTTCATC -3'
Posted On 2019-09-13