Mutagenetix > Incidental Mutation

Incidental Mutation 'R7352:Map4k4'

570606

Institutional Source

Beutler Lab

Gene Symbol

Map4k4

Ensembl Gene

ENSMUSG00000026074

Gene Name

mitogen-activated protein kinase kinase kinase kinase 4

Synonyms

9430080K19Rik, Nik

MMRRC Submission

045438-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R7352 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

39940073-40065470 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 40001387 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Valine to Aspartic acid at position 58 (V58D)

Ref Sequence

ENSEMBL: ENSMUSP00000141862 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000163854] [ENSMUST00000168431] [ENSMUST00000191761] [ENSMUST00000192509] [ENSMUST00000193682] [ENSMUST00000195259] [ENSMUST00000195636] [ENSMUST00000195860]

AlphaFold

no structure available at present

Predicted Effect

unknown
Transcript: ENSMUST00000163854
AA Change: V58D

SMART Domains

Protein: ENSMUSP00000126961
Gene: ENSMUSG00000026074
AA Change: V58D

Domain	Start	End	E-Value	Type
S_TKc	25	289	6.87e-95	SMART
low complexity region	318	342	N/A	INTRINSIC
coiled coil region	357	494	N/A	INTRINSIC
low complexity region	503	512	N/A	INTRINSIC
low complexity region	687	698	N/A	INTRINSIC
low complexity region	721	747	N/A	INTRINSIC
low complexity region	754	763	N/A	INTRINSIC
low complexity region	811	837	N/A	INTRINSIC
low complexity region	891	904	N/A	INTRINSIC
low complexity region	919	929	N/A	INTRINSIC
CNH	970	1268	2.76e-127	SMART

Predicted Effect

SMART Domains

Protein: ENSMUSP00000129796
Gene: ENSMUSG00000026074
AA Change: V58D

Domain	Start	End	E-Value	Type
S_TKc	25	289	6.87e-95	SMART
low complexity region	318	342	N/A	INTRINSIC
coiled coil region	357	494	N/A	INTRINSIC
low complexity region	503	512	N/A	INTRINSIC
low complexity region	633	644	N/A	INTRINSIC
low complexity region	667	693	N/A	INTRINSIC
low complexity region	700	709	N/A	INTRINSIC
low complexity region	757	783	N/A	INTRINSIC
low complexity region	837	850	N/A	INTRINSIC
low complexity region	865	875	N/A	INTRINSIC
CNH	916	1214	2.76e-127	SMART

Predicted Effect

unknown
Transcript: ENSMUST00000191761
AA Change: V58D

SMART Domains

Protein: ENSMUSP00000141332
Gene: ENSMUSG00000026074
AA Change: V58D

Domain	Start	End	E-Value	Type
S_TKc	25	289	3.4e-97	SMART
low complexity region	318	342	N/A	INTRINSIC
coiled coil region	357	404	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000192509
AA Change: V58D

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000141665
Gene: ENSMUSG00000026074
AA Change: V58D

Domain	Start	End	E-Value	Type
S_TKc	25	289	6.87e-95	SMART
low complexity region	318	342	N/A	INTRINSIC
coiled coil region	357	494	N/A	INTRINSIC
low complexity region	503	512	N/A	INTRINSIC
low complexity region	633	644	N/A	INTRINSIC
low complexity region	667	693	N/A	INTRINSIC
low complexity region	700	709	N/A	INTRINSIC
low complexity region	757	783	N/A	INTRINSIC
low complexity region	837	850	N/A	INTRINSIC
low complexity region	865	875	N/A	INTRINSIC
CNH	916	1214	2.76e-127	SMART

Predicted Effect

unknown
Transcript: ENSMUST00000193682
AA Change: V58D

SMART Domains

Protein: ENSMUSP00000141862
Gene: ENSMUSG00000026074
AA Change: V58D

Domain	Start	End	E-Value	Type
S_TKc	25	289	6.87e-95	SMART
low complexity region	318	342	N/A	INTRINSIC
coiled coil region	357	494	N/A	INTRINSIC
low complexity region	503	512	N/A	INTRINSIC
low complexity region	556	567	N/A	INTRINSIC
low complexity region	590	616	N/A	INTRINSIC
low complexity region	623	632	N/A	INTRINSIC
low complexity region	680	706	N/A	INTRINSIC
low complexity region	785	814	N/A	INTRINSIC
low complexity region	824	837	N/A	INTRINSIC
low complexity region	852	862	N/A	INTRINSIC
CNH	903	1201	2.76e-127	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000195259
AA Change: V58D

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000142056
Gene: ENSMUSG00000026074
AA Change: V58D

Domain	Start	End	E-Value	Type
S_TKc	25	289	6.87e-95	SMART
low complexity region	318	342	N/A	INTRINSIC
coiled coil region	357	494	N/A	INTRINSIC
low complexity region	503	512	N/A	INTRINSIC
low complexity region	610	621	N/A	INTRINSIC
low complexity region	644	670	N/A	INTRINSIC
low complexity region	677	686	N/A	INTRINSIC
low complexity region	731	757	N/A	INTRINSIC
low complexity region	811	824	N/A	INTRINSIC
low complexity region	839	849	N/A	INTRINSIC
CNH	890	1188	2.76e-127	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000195636
AA Change: V58D

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000141613
Gene: ENSMUSG00000026074
AA Change: V58D

Domain	Start	End	E-Value	Type
S_TKc	25	289	3.4e-97	SMART
low complexity region	318	342	N/A	INTRINSIC
coiled coil region	357	494	N/A	INTRINSIC
low complexity region	503	512	N/A	INTRINSIC
low complexity region	610	621	N/A	INTRINSIC
low complexity region	644	670	N/A	INTRINSIC
low complexity region	677	686	N/A	INTRINSIC
low complexity region	731	757	N/A	INTRINSIC
low complexity region	836	865	N/A	INTRINSIC
low complexity region	875	888	N/A	INTRINSIC
low complexity region	903	913	N/A	INTRINSIC
CNH	954	1252	1.4e-129	SMART

Predicted Effect

unknown
Transcript: ENSMUST00000195860
AA Change: V58D

SMART Domains

Protein: ENSMUSP00000141400
Gene: ENSMUSG00000026074
AA Change: V58D

Domain	Start	End	E-Value	Type
S_TKc	25	289	6.87e-95	SMART
low complexity region	318	342	N/A	INTRINSIC
coiled coil region	357	494	N/A	INTRINSIC
low complexity region	503	512	N/A	INTRINSIC
low complexity region	687	698	N/A	INTRINSIC
low complexity region	721	747	N/A	INTRINSIC
low complexity region	754	763	N/A	INTRINSIC
low complexity region	811	837	N/A	INTRINSIC
low complexity region	891	904	N/A	INTRINSIC
low complexity region	919	929	N/A	INTRINSIC
CNH	970	1268	2.76e-127	SMART

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.8%
20x: 99.4%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase has been shown to specifically activate MAPK8/JNK. The activation of MAPK8 by this kinase is found to be inhibited by the dominant-negative mutants of MAP3K7/TAK1, MAP2K4/MKK4, and MAP2K7/MKK7, which suggests that this kinase may function through the MAP3K7-MAP2K4-MAP2K7 kinase cascade, and mediate the TNF-alpha signaling pathway. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]
PHENOTYPE: Mice homozygous for disruptions in this gene die as embryos around day E9.5-10.5. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 100 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca17	C	T	17: 24,508,028 (GRCm39)	W1124*	probably null	Het
Acap3	G	A	4: 155,990,168 (GRCm39)	V783I	possibly damaging	Het
Alkbh8	A	G	9: 3,345,796 (GRCm39)	K172R	probably damaging	Het
Ankrd28	A	G	14: 31,429,998 (GRCm39)	L860P	probably damaging	Het
Arap3	G	A	18: 38,106,331 (GRCm39)	T1504I	probably benign	Het
Atad5	T	C	11: 79,994,169 (GRCm39)		probably null	Het
Atp8a2	A	G	14: 60,028,653 (GRCm39)	V938A	probably benign	Het
AW551984	T	C	9: 39,504,221 (GRCm39)	M582V	probably benign	Het
Axdnd1	C	T	1: 156,210,047 (GRCm39)	V408I	possibly damaging	Het
Babam2	T	A	5: 32,164,594 (GRCm39)	Y326*	probably null	Het
Baiap2l1	T	C	5: 144,261,436 (GRCm39)	M20V	probably benign	Het
Bex6	A	G	16: 32,005,034 (GRCm39)		probably benign	Het
Bicc1	G	T	10: 70,783,730 (GRCm39)	T469K	probably benign	Het
Cad	T	A	5: 31,215,422 (GRCm39)	S78T	probably damaging	Het
Ccdc171	T	A	4: 83,736,260 (GRCm39)	D1210E	possibly damaging	Het
Ccnd1	T	C	7: 144,491,124 (GRCm39)	I161V	possibly damaging	Het
Cdc42bpb	A	T	12: 111,265,745 (GRCm39)	F1396I	probably damaging	Het
Cep170	A	T	1: 176,597,423 (GRCm39)	D373E	probably benign	Het
Chrna4	A	T	2: 180,679,267 (GRCm39)	I60N	probably damaging	Het
Cntn2	T	C	1: 132,450,137 (GRCm39)	T586A	probably benign	Het
Csmd2	G	T	4: 128,451,429 (GRCm39)	V3396F		Het
Cyp3a44	T	A	5: 145,740,498 (GRCm39)	L47F	probably benign	Het
D7Ertd443e	A	T	7: 133,951,123 (GRCm39)	H183Q	probably benign	Het
Ddx27	T	C	2: 166,871,433 (GRCm39)	V510A	probably benign	Het
Dgat1	G	T	15: 76,387,024 (GRCm39)	C396*	probably null	Het
Dmpk	A	G	7: 18,819,997 (GRCm39)	S83G	probably damaging	Het
Dsc2	T	G	18: 20,168,392 (GRCm39)	D689A	probably benign	Het
Eppk1	A	T	15: 75,990,618 (GRCm39)	W2088R	probably benign	Het
Exph5	A	C	9: 53,287,022 (GRCm39)	I1368L	probably benign	Het
Fadd	T	C	7: 144,134,396 (GRCm39)	K163R	probably benign	Het
Fam149a	T	A	8: 45,794,034 (GRCm39)	Q759L	probably damaging	Het
Foxo6	A	T	4: 120,125,448 (GRCm39)	M449K	probably benign	Het
Gad1	T	C	2: 70,425,094 (GRCm39)	F463L	probably benign	Het
Gad2	T	A	2: 22,513,835 (GRCm39)	F91I	probably benign	Het
Gcc2	T	C	10: 58,116,520 (GRCm39)		probably null	Het
Gjd3	A	C	11: 102,691,278 (GRCm39)	C242G	probably damaging	Het
Gkn1	T	C	6: 87,325,100 (GRCm39)	E103G	possibly damaging	Het
Gm867	T	C	10: 75,775,670 (GRCm39)	T39A	probably benign	Het
Gpr108	A	G	17: 57,543,944 (GRCm39)	Y421H	probably damaging	Het
Gramd1b	A	C	9: 40,219,289 (GRCm39)	V508G	probably damaging	Het
Grm1	A	T	10: 10,595,237 (GRCm39)	I797N	probably damaging	Het
Ice1	G	A	13: 70,754,221 (GRCm39)	Q622*	probably null	Het
Kcnb2	T	A	1: 15,780,835 (GRCm39)	V569E	probably benign	Het
Ksr2	T	G	5: 117,827,706 (GRCm39)	I495S	probably benign	Het
Litaf	A	T	16: 10,781,217 (GRCm39)	M94K	probably damaging	Het
Lmod2	T	C	6: 24,598,110 (GRCm39)	S77P	possibly damaging	Het
Lrp2	G	T	2: 69,302,741 (GRCm39)	S2996Y	probably benign	Het
Ltf	T	C	9: 110,857,518 (GRCm39)	S436P	probably benign	Het
Mapkapk3	C	A	9: 107,134,269 (GRCm39)	D332Y	possibly damaging	Het
Mroh1	C	A	15: 76,335,674 (GRCm39)	P1439Q	probably benign	Het
Ncor1	A	G	11: 62,224,737 (GRCm39)	V841A	probably damaging	Het
Nrxn3	A	G	12: 88,817,063 (GRCm39)	M248V	probably benign	Het
Olig2	A	G	16: 91,023,577 (GRCm39)	D97G	probably benign	Het
Or4a2	C	A	2: 89,248,311 (GRCm39)	G149W	probably damaging	Het
Or4a39	T	C	2: 89,236,806 (GRCm39)	M206V	probably benign	Het
Or4p8	T	A	2: 88,727,062 (GRCm39)	N293I	probably damaging	Het
Or5b95	A	G	19: 12,658,115 (GRCm39)	I214M	possibly damaging	Het
Or5g23	T	C	2: 85,439,128 (GRCm39)	N42S	probably damaging	Het
Or6f2	A	T	7: 139,756,438 (GRCm39)	Y135F	probably damaging	Het
Pcdha11	T	A	18: 37,139,898 (GRCm39)	V509E	probably damaging	Het
Pglyrp3	G	A	3: 91,921,947 (GRCm39)	W5*	probably null	Het
Phf14	A	T	6: 11,961,637 (GRCm39)	N425Y	probably damaging	Het
Phf3	G	T	1: 30,843,407 (GRCm39)	R1851S	possibly damaging	Het
Pigs	A	G	11: 78,219,638 (GRCm39)	T39A	probably damaging	Het
Plin4	A	G	17: 56,411,427 (GRCm39)	M868T	probably benign	Het
Pnma2	A	G	14: 67,153,870 (GRCm39)	Q98R	possibly damaging	Het
Ppp4r3a	G	A	12: 101,008,091 (GRCm39)	T782I	probably damaging	Het
Pramel52-ps	A	T	5: 94,531,743 (GRCm39)	N209I	probably damaging	Het
Prickle2	T	G	6: 92,387,871 (GRCm39)	E566D	probably benign	Het
Pvr	A	G	7: 19,644,541 (GRCm39)	I331T	possibly damaging	Het
Ranbp3l	A	T	15: 8,997,842 (GRCm39)		probably benign	Het
Rapgef4	T	A	2: 72,010,435 (GRCm39)	I331N	probably damaging	Het
Reg3g	C	T	6: 78,443,842 (GRCm39)	W122*	probably null	Het
Rigi	T	A	4: 40,239,668 (GRCm39)	Q10L	probably benign	Het
Rlbp1	A	T	7: 79,031,428 (GRCm39)	I100N	probably damaging	Het
Rnf213	T	A	11: 119,334,405 (GRCm39)	Y3206N		Het
Rsf1	CGGCGGCGG	CGGCGGCGGGGGCGGCGG	7: 97,229,133 (GRCm39)		probably benign	Het
Rtl6	G	T	15: 84,441,181 (GRCm39)	R72S	possibly damaging	Het
Rufy3	T	A	5: 88,785,053 (GRCm39)	D408E	possibly damaging	Het
Scn3a	T	C	2: 65,356,045 (GRCm39)	I230V	possibly damaging	Het
Slc23a4	A	T	6: 34,925,214 (GRCm39)	I507N	possibly damaging	Het
Slc4a8	A	G	15: 100,688,865 (GRCm39)	T392A	probably damaging	Het
Spaca7	T	A	8: 12,635,742 (GRCm39)	I86K	probably benign	Het
Syt10	G	T	15: 89,698,659 (GRCm39)	D228E	probably benign	Het
Tbx3	T	A	5: 119,815,625 (GRCm39)	N308K	probably benign	Het
Telo2	A	G	17: 25,321,043 (GRCm39)	V724A	probably damaging	Het
Tfip11	T	C	5: 112,481,134 (GRCm39)	V370A	probably benign	Het
Thbs3	A	G	3: 89,132,587 (GRCm39)	E843G	probably benign	Het
Tmprss11g	T	C	5: 86,644,401 (GRCm39)	I148V	not run	Het
Trbv14	T	A	6: 41,112,429 (GRCm39)	D75E	possibly damaging	Het
Trio	A	G	15: 27,732,962 (GRCm39)	V2973A	probably damaging	Het
Ttn	T	C	2: 76,624,828 (GRCm39)	D15250G	probably damaging	Het
Ubr2	G	A	17: 47,241,352 (GRCm39)	T1734I	probably benign	Het
Ush2a	G	T	1: 188,198,321 (GRCm39)	A1462S	probably benign	Het
Ushbp1	T	G	8: 71,841,525 (GRCm39)	T435P	possibly damaging	Het
Vps13c	T	C	9: 67,747,728 (GRCm39)	V7A	possibly damaging	Het
Wnk4	T	C	11: 101,155,244 (GRCm39)	V385A	probably damaging	Het
Wnt2b	C	T	3: 104,854,493 (GRCm39)	V322I	probably benign	Het
Zbtb8a	C	T	4: 129,253,874 (GRCm39)	V207M	probably benign	Het
Zc3hav1l	A	G	6: 38,275,916 (GRCm39)	M87T	probably benign	Het

Other mutations in Map4k4

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00328:Map4k4	APN	1	40,043,976 (GRCm39)	missense	probably damaging	0.99
IGL00417:Map4k4	APN	1	40,053,692 (GRCm39)	missense	possibly damaging	0.92
IGL00516:Map4k4	APN	1	40,053,762 (GRCm39)	missense	probably damaging	1.00
IGL01545:Map4k4	APN	1	40,053,389 (GRCm39)	splice site	probably benign
IGL02092:Map4k4	APN	1	40,063,508 (GRCm39)	missense	probably damaging	1.00
IGL02092:Map4k4	APN	1	40,025,943 (GRCm39)	missense	probably benign	0.12
IGL02570:Map4k4	APN	1	40,019,739 (GRCm39)	missense	probably benign	0.06
IGL02626:Map4k4	APN	1	40,053,257 (GRCm39)	splice site	probably benign
IGL02993:Map4k4	APN	1	40,053,348 (GRCm39)	missense	probably damaging	0.98
IGL03178:Map4k4	APN	1	40,025,853 (GRCm39)	missense	possibly damaging	0.63
tank	UTSW	1	40,044,024 (GRCm39)	missense	possibly damaging	0.93
IGL02835:Map4k4	UTSW	1	40,049,760 (GRCm39)	missense	probably damaging	0.99
R0496:Map4k4	UTSW	1	40,045,982 (GRCm39)	missense	probably damaging	0.99
R0498:Map4k4	UTSW	1	40,029,338 (GRCm39)	missense	probably benign	0.22
R0588:Map4k4	UTSW	1	40,044,024 (GRCm39)	missense	possibly damaging	0.93
R0674:Map4k4	UTSW	1	40,042,975 (GRCm39)	missense	probably damaging	1.00
R1205:Map4k4	UTSW	1	40,043,004 (GRCm39)	missense	probably damaging	1.00
R1349:Map4k4	UTSW	1	40,060,319 (GRCm39)	missense	probably damaging	1.00
R1615:Map4k4	UTSW	1	40,045,990 (GRCm39)	splice site	probably benign
R1763:Map4k4	UTSW	1	40,039,917 (GRCm39)	splice site	probably benign
R1800:Map4k4	UTSW	1	40,062,620 (GRCm39)	missense	probably damaging	1.00
R1893:Map4k4	UTSW	1	40,040,717 (GRCm39)	missense	probably benign	0.08
R2411:Map4k4	UTSW	1	40,046,656 (GRCm39)	missense	probably damaging	0.96
R2851:Map4k4	UTSW	1	40,039,915 (GRCm39)	splice site	probably benign
R2852:Map4k4	UTSW	1	40,039,915 (GRCm39)	splice site	probably benign
R2987:Map4k4	UTSW	1	40,025,925 (GRCm39)	missense	probably damaging	1.00
R3087:Map4k4	UTSW	1	40,060,242 (GRCm39)	critical splice acceptor site	probably null
R3688:Map4k4	UTSW	1	40,024,331 (GRCm39)	splice site	probably null
R4075:Map4k4	UTSW	1	40,062,622 (GRCm39)	missense	probably damaging	0.96
R4304:Map4k4	UTSW	1	40,013,132 (GRCm39)	missense	possibly damaging	0.74
R4564:Map4k4	UTSW	1	40,028,135 (GRCm39)	missense	probably damaging	1.00
R4569:Map4k4	UTSW	1	40,039,698 (GRCm39)	missense	probably damaging	1.00
R4613:Map4k4	UTSW	1	40,056,351 (GRCm39)	missense	probably benign	0.05
R4715:Map4k4	UTSW	1	40,058,724 (GRCm39)	missense	probably damaging	1.00
R4788:Map4k4	UTSW	1	40,043,076 (GRCm39)	missense	probably benign	0.01
R4926:Map4k4	UTSW	1	40,056,385 (GRCm39)	missense	probably damaging	1.00
R4943:Map4k4	UTSW	1	40,058,754 (GRCm39)	missense	probably damaging	0.99
R5033:Map4k4	UTSW	1	40,046,662 (GRCm39)	missense	probably damaging	0.99
R5177:Map4k4	UTSW	1	40,025,922 (GRCm39)	missense	probably damaging	1.00
R5297:Map4k4	UTSW	1	40,001,377 (GRCm39)	missense	probably damaging	1.00
R5844:Map4k4	UTSW	1	40,039,036 (GRCm39)	splice site	probably benign
R5952:Map4k4	UTSW	1	40,039,082 (GRCm39)	unclassified	probably benign
R6111:Map4k4	UTSW	1	40,050,822 (GRCm39)	missense	probably benign	0.00
R6125:Map4k4	UTSW	1	40,043,125 (GRCm39)	missense	possibly damaging	0.77
R6838:Map4k4	UTSW	1	40,015,882 (GRCm39)	missense	probably damaging	1.00
R6927:Map4k4	UTSW	1	40,050,842 (GRCm39)	missense	probably benign	0.00
R7008:Map4k4	UTSW	1	40,028,131 (GRCm39)	missense	probably benign	0.44
R7164:Map4k4	UTSW	1	40,013,132 (GRCm39)	missense	possibly damaging	0.74
R7195:Map4k4	UTSW	1	40,058,829 (GRCm39)	missense	possibly damaging	0.93
R7589:Map4k4	UTSW	1	40,060,251 (GRCm39)	nonsense	probably null
R7816:Map4k4	UTSW	1	40,053,368 (GRCm39)	missense	possibly damaging	0.53
R7869:Map4k4	UTSW	1	40,013,204 (GRCm39)	missense	unknown
R8013:Map4k4	UTSW	1	40,001,372 (GRCm39)	missense	unknown
R8145:Map4k4	UTSW	1	40,039,694 (GRCm39)	missense
R8154:Map4k4	UTSW	1	40,060,302 (GRCm39)	nonsense	probably null
R8254:Map4k4	UTSW	1	40,045,835 (GRCm39)	missense	probably damaging	0.99
R8266:Map4k4	UTSW	1	40,050,813 (GRCm39)	missense	possibly damaging	0.53
R8375:Map4k4	UTSW	1	40,063,801 (GRCm39)	missense	possibly damaging	0.73
R8487:Map4k4	UTSW	1	40,028,136 (GRCm39)	missense	probably damaging	1.00
R8699:Map4k4	UTSW	1	40,015,910 (GRCm39)	missense	unknown
R8726:Map4k4	UTSW	1	40,043,142 (GRCm39)	missense	possibly damaging	0.95
R8907:Map4k4	UTSW	1	40,058,770 (GRCm39)	missense	probably damaging	0.97
R8956:Map4k4	UTSW	1	40,039,840 (GRCm39)	missense	probably benign	0.11
R8963:Map4k4	UTSW	1	40,039,740 (GRCm39)	missense	probably damaging	1.00
R9091:Map4k4	UTSW	1	40,042,923 (GRCm39)	missense	probably benign	0.02
R9234:Map4k4	UTSW	1	40,029,261 (GRCm39)	missense	unknown
R9270:Map4k4	UTSW	1	40,042,923 (GRCm39)	missense	probably benign	0.02
R9438:Map4k4	UTSW	1	40,045,952 (GRCm39)	missense	probably damaging	0.99
R9689:Map4k4	UTSW	1	40,058,722 (GRCm39)	missense	possibly damaging	0.95
R9771:Map4k4	UTSW	1	40,025,877 (GRCm39)	missense	unknown

Predicted Primers

PCR Primer
(F):5'- CGTTGTTCTATTATGAGCATGCAC -3'
(R):5'- TGTGGCCAAAGATGACATCAC -3'

Sequencing Primer
(F):5'- CTATTATGAGCATGCACTTAAAAGC -3'
(R):5'- TATCTCAACAACTCAAGTCGCCTGG -3'

Posted On

2019-09-13