Mutagenetix > Incidental Mutation

Incidental Mutation 'R7362:Vmn2r82'

571426

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r82

Ensembl Gene

ENSMUSG00000091468

Gene Name

vomeronasal 2, receptor 82

Synonyms

EG624845

MMRRC Submission

045375-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.068) question?

Stock #

R7362 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

79192425-79232600 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 79232451 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Methionine to Leucine at position 817 (M817L)

Ref Sequence

ENSEMBL: ENSMUSP00000130114 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000170596]

AlphaFold

G3UWA2

Predicted Effect

probably benign
Transcript: ENSMUST00000170596
AA Change: M817L

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)

SMART Domains

Protein: ENSMUSP00000130114
Gene: ENSMUSG00000091468
AA Change: M817L

Domain	Start	End	E-Value	Type
signal peptide	1	18	N/A	INTRINSIC
Pfam:ANF_receptor	79	474	6e-35	PFAM
Pfam:NCD3G	517	570	9.3e-22	PFAM
Pfam:7tm_3	603	838	6.5e-49	PFAM

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.7%
20x: 99.1%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 52 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adprhl1	C	T	8: 13,295,534 (GRCm39)	R193K	probably damaging	Het
Aoc1	G	T	6: 48,882,345 (GRCm39)	V74L	probably benign	Het
Bfsp1	G	C	2: 143,668,795 (GRCm39)	P601A	probably benign	Het
Bicd1	A	C	6: 149,385,591 (GRCm39)	K108T	probably benign	Het
Btbd18	C	T	2: 84,491,887 (GRCm39)	Q23*	probably null	Het
Ccser1	A	G	6: 61,787,864 (GRCm39)	I227M	unknown	Het
Cd151	G	A	7: 141,049,502 (GRCm39)	V70I	probably benign	Het
Cdh10	A	G	15: 18,899,780 (GRCm39)	T36A	probably benign	Het
Crb2	A	G	2: 37,680,211 (GRCm39)	T380A	probably benign	Het
Csmd3	A	C	15: 47,619,388 (GRCm39)	S1829A	possibly damaging	Het
Dgat2	A	T	7: 98,803,843 (GRCm39)	H359Q	probably damaging	Het
Dhdds	T	C	4: 133,698,441 (GRCm39)	T298A	probably benign	Het
Egfem1	A	G	3: 29,206,069 (GRCm39)	Q102R	probably benign	Het
Eps15	T	A	4: 109,223,439 (GRCm39)		probably null	Het
Ern1	T	A	11: 106,327,949 (GRCm39)	D61V	probably damaging	Het
Fcamr	G	A	1: 130,741,760 (GRCm39)	R511Q	possibly damaging	Het
Grhpr	T	C	4: 44,987,255 (GRCm39)	V213A	probably benign	Het
Gsx2	A	G	5: 75,236,765 (GRCm39)	D115G	possibly damaging	Het
Hoxd3	A	G	2: 74,574,563 (GRCm39)	I70V	possibly damaging	Het
Inpp5a	A	G	7: 139,158,296 (GRCm39)	I408V	probably benign	Het
Kansl3	A	T	1: 36,383,208 (GRCm39)	D759E	possibly damaging	Het
Lrrc37a	T	G	11: 103,348,335 (GRCm39)	T2787P	unknown	Het
Mier3	G	A	13: 111,841,783 (GRCm39)	G115S	possibly damaging	Het
Mkln1	T	C	6: 31,445,103 (GRCm39)	I333T	probably benign	Het
Myt1	T	A	2: 181,439,033 (GRCm39)	V227D	probably benign	Het
Or1a1	T	C	11: 74,086,412 (GRCm39)	F28L	probably benign	Het
Or52s1	T	A	7: 102,861,861 (GRCm39)	Y265N	probably damaging	Het
Or7e171-ps1	C	T	9: 19,853,527 (GRCm39)	D70N	probably damaging	Het
Pcf11	A	G	7: 92,302,453 (GRCm39)	L1219P	possibly damaging	Het
Pde6g	T	C	11: 120,338,950 (GRCm39)	E80G	probably damaging	Het
Pkd1l3	GACACCTGCATCCAGCAGCCCAACAAACATGACATCAGACACACCTGCATCCAGCAGCCCAACAAACATGACATCAGACACACCTGCATCCAGCAGCCCAACAAACATGACATCAGACACACCTGCATCCAGCAGCCCA	GACACCTGCATCCAGCAGCCCAACAAACATGACATCAGACACACCTGCATCCAGCAGCCCAACAAACATGACATCAGACACACCTGCATCCAGCAGCCCA	8: 110,350,827 (GRCm39)		probably benign	Het
Ppp3cb	T	A	14: 20,573,719 (GRCm39)	Q304L	probably benign	Het
Prr19	T	C	7: 25,003,343 (GRCm39)	L319P	probably damaging	Het
Prss23	C	T	7: 89,158,972 (GRCm39)	A366T	probably damaging	Het
Rock2	T	C	12: 17,008,422 (GRCm39)	L560P	probably damaging	Het
Simc1	A	T	13: 54,687,517 (GRCm39)	R95S	probably damaging	Het
Slc10a6	A	T	5: 103,776,992 (GRCm39)	V36E	probably damaging	Het
Slco1c1	A	T	6: 141,515,189 (GRCm39)	T695S	probably benign	Het
Spata31h1	T	A	10: 82,128,831 (GRCm39)	Q1393L	possibly damaging	Het
Ssh2	T	C	11: 77,340,476 (GRCm39)	F543L	probably benign	Het
Tecpr2	T	A	12: 110,907,910 (GRCm39)	V999D	possibly damaging	Het
Tnfsf10	A	T	3: 27,389,497 (GRCm39)	Y186F	probably damaging	Het
Tnk2	A	T	16: 32,494,338 (GRCm39)		probably null	Het
Tram1	T	C	1: 13,659,832 (GRCm39)	M39V	probably benign	Het
Uck2	C	T	1: 167,065,211 (GRCm39)	V36I	possibly damaging	Het
Ugcg	T	A	4: 59,217,109 (GRCm39)	M211K	probably damaging	Het
Vash2	A	T	1: 190,692,496 (GRCm39)	S226R	probably damaging	Het
Vmn1r172	T	A	7: 23,359,841 (GRCm39)	M242K	probably damaging	Het
Vwa5b1	T	C	4: 138,321,623 (GRCm39)	N390S	probably damaging	Het
Wdr73	T	A	7: 80,550,451 (GRCm39)	D17V	probably damaging	Het
Wdr91	A	T	6: 34,866,050 (GRCm39)	S501T	possibly damaging	Het
Zfp454	A	G	11: 50,777,194 (GRCm39)		probably null	Het

Other mutations in Vmn2r82

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01800:Vmn2r82	APN	10	79,192,581 (GRCm39)	missense	probably benign	0.03
IGL01860:Vmn2r82	APN	10	79,214,691 (GRCm39)	missense	probably benign	0.18
IGL01927:Vmn2r82	APN	10	79,213,906 (GRCm39)	missense	probably damaging	1.00
IGL01929:Vmn2r82	APN	10	79,214,545 (GRCm39)	missense	probably damaging	1.00
IGL02028:Vmn2r82	APN	10	79,215,057 (GRCm39)	missense	probably benign
IGL02112:Vmn2r82	APN	10	79,231,833 (GRCm39)	missense	probably benign	0.19
IGL02632:Vmn2r82	APN	10	79,192,542 (GRCm39)	missense	probably benign	0.45
IGL02665:Vmn2r82	APN	10	79,215,205 (GRCm39)	missense	probably damaging	0.99
IGL02716:Vmn2r82	APN	10	79,213,678 (GRCm39)	missense	probably benign	0.20
IGL03030:Vmn2r82	APN	10	79,217,149 (GRCm39)	missense	possibly damaging	0.85
IGL03190:Vmn2r82	APN	10	79,192,643 (GRCm39)	splice site	probably null
IGL03349:Vmn2r82	APN	10	79,213,703 (GRCm39)	missense	probably benign	0.25
IGL03048:Vmn2r82	UTSW	10	79,232,460 (GRCm39)	missense	probably damaging	0.98
R0080:Vmn2r82	UTSW	10	79,232,339 (GRCm39)	missense	probably benign	0.00
R0193:Vmn2r82	UTSW	10	79,217,129 (GRCm39)	missense	probably damaging	1.00
R0217:Vmn2r82	UTSW	10	79,214,634 (GRCm39)	missense	possibly damaging	0.46
R0285:Vmn2r82	UTSW	10	79,232,391 (GRCm39)	missense	probably damaging	1.00
R1193:Vmn2r82	UTSW	10	79,213,739 (GRCm39)	nonsense	probably null
R1385:Vmn2r82	UTSW	10	79,232,325 (GRCm39)	nonsense	probably null
R1386:Vmn2r82	UTSW	10	79,214,545 (GRCm39)	missense	probably damaging	1.00
R1442:Vmn2r82	UTSW	10	79,215,201 (GRCm39)	missense	probably benign	0.03
R1467:Vmn2r82	UTSW	10	79,232,133 (GRCm39)	missense	probably benign	0.00
R1467:Vmn2r82	UTSW	10	79,232,133 (GRCm39)	missense	probably benign	0.00
R1518:Vmn2r82	UTSW	10	79,214,702 (GRCm39)	missense	probably damaging	1.00
R1538:Vmn2r82	UTSW	10	79,192,578 (GRCm39)	missense	possibly damaging	0.92
R1607:Vmn2r82	UTSW	10	79,215,253 (GRCm39)	missense	possibly damaging	0.67
R1812:Vmn2r82	UTSW	10	79,215,046 (GRCm39)	missense	probably benign	0.33
R1906:Vmn2r82	UTSW	10	79,232,344 (GRCm39)	missense	probably damaging	1.00
R1954:Vmn2r82	UTSW	10	79,231,890 (GRCm39)	missense	probably damaging	1.00
R1972:Vmn2r82	UTSW	10	79,214,680 (GRCm39)	missense	probably damaging	1.00
R2093:Vmn2r82	UTSW	10	79,231,813 (GRCm39)	missense	probably benign	0.30
R2156:Vmn2r82	UTSW	10	79,214,722 (GRCm39)	missense	probably damaging	1.00
R2202:Vmn2r82	UTSW	10	79,192,519 (GRCm39)	missense	probably benign
R2442:Vmn2r82	UTSW	10	79,221,210 (GRCm39)	missense	probably damaging	1.00
R2444:Vmn2r82	UTSW	10	79,213,702 (GRCm39)	missense	possibly damaging	0.65
R2857:Vmn2r82	UTSW	10	79,217,090 (GRCm39)	missense	probably damaging	0.98
R2858:Vmn2r82	UTSW	10	79,217,090 (GRCm39)	missense	probably damaging	0.98
R2884:Vmn2r82	UTSW	10	79,232,082 (GRCm39)	missense	probably benign	0.00
R2886:Vmn2r82	UTSW	10	79,232,082 (GRCm39)	missense	probably benign	0.00
R4369:Vmn2r82	UTSW	10	79,231,914 (GRCm39)	missense	probably benign	0.01
R4445:Vmn2r82	UTSW	10	79,214,874 (GRCm39)	missense	possibly damaging	0.87
R4589:Vmn2r82	UTSW	10	79,192,548 (GRCm39)	missense	probably damaging	1.00
R4703:Vmn2r82	UTSW	10	79,214,641 (GRCm39)	missense	probably damaging	1.00
R4908:Vmn2r82	UTSW	10	79,214,589 (GRCm39)	missense	probably benign	0.00
R4937:Vmn2r82	UTSW	10	79,215,010 (GRCm39)	missense	probably benign	0.01
R5199:Vmn2r82	UTSW	10	79,231,921 (GRCm39)	missense	probably damaging	1.00
R5391:Vmn2r82	UTSW	10	79,192,491 (GRCm39)	missense	probably null	0.01
R5601:Vmn2r82	UTSW	10	79,232,025 (GRCm39)	missense	probably damaging	1.00
R5635:Vmn2r82	UTSW	10	79,214,652 (GRCm39)	missense	probably benign	0.33
R6065:Vmn2r82	UTSW	10	79,221,210 (GRCm39)	missense	probably damaging	1.00
R6074:Vmn2r82	UTSW	10	79,232,377 (GRCm39)	missense	probably damaging	1.00
R6340:Vmn2r82	UTSW	10	79,231,727 (GRCm39)	missense	probably benign	0.00
R6474:Vmn2r82	UTSW	10	79,214,871 (GRCm39)	missense	possibly damaging	0.55
R6995:Vmn2r82	UTSW	10	79,232,377 (GRCm39)	missense	probably damaging	1.00
R7111:Vmn2r82	UTSW	10	79,214,605 (GRCm39)	missense	probably benign	0.22
R7212:Vmn2r82	UTSW	10	79,215,268 (GRCm39)	missense	probably benign	0.00
R7335:Vmn2r82	UTSW	10	79,214,722 (GRCm39)	missense	probably damaging	1.00
R7353:Vmn2r82	UTSW	10	79,232,452 (GRCm39)	missense	probably benign	0.11
R7354:Vmn2r82	UTSW	10	79,192,464 (GRCm39)	missense	probably benign	0.00
R7378:Vmn2r82	UTSW	10	79,232,276 (GRCm39)	nonsense	probably null
R7430:Vmn2r82	UTSW	10	79,217,087 (GRCm39)	missense	probably damaging	1.00
R7509:Vmn2r82	UTSW	10	79,231,842 (GRCm39)	missense	possibly damaging	0.82
R7874:Vmn2r82	UTSW	10	79,232,345 (GRCm39)	missense	probably damaging	1.00
R7943:Vmn2r82	UTSW	10	79,232,079 (GRCm39)	missense	possibly damaging	0.74
R8158:Vmn2r82	UTSW	10	79,213,636 (GRCm39)	missense	probably benign	0.12
R8324:Vmn2r82	UTSW	10	79,214,727 (GRCm39)	nonsense	probably null
R8340:Vmn2r82	UTSW	10	79,217,036 (GRCm39)	missense	probably benign	0.00
R8787:Vmn2r82	UTSW	10	79,213,894 (GRCm39)	missense	probably damaging	1.00
R8929:Vmn2r82	UTSW	10	79,232,541 (GRCm39)	missense	probably benign	0.00
R9018:Vmn2r82	UTSW	10	79,232,539 (GRCm39)	missense	probably damaging	1.00
R9399:Vmn2r82	UTSW	10	79,214,768 (GRCm39)	nonsense	probably null
R9517:Vmn2r82	UTSW	10	79,213,641 (GRCm39)	nonsense	probably null
R9587:Vmn2r82	UTSW	10	79,214,936 (GRCm39)	missense	possibly damaging	0.70
R9602:Vmn2r82	UTSW	10	79,214,880 (GRCm39)	missense	probably benign	0.07
Z1088:Vmn2r82	UTSW	10	79,192,456 (GRCm39)	missense	probably damaging	1.00
Z1177:Vmn2r82	UTSW	10	79,232,369 (GRCm39)	missense	probably damaging	1.00
Z1177:Vmn2r82	UTSW	10	79,192,429 (GRCm39)	missense	probably benign	0.03

Predicted Primers

PCR Primer
(F):5'- TACTGCACTCTGGCTTACCTGG -3'
(R):5'- TTGACTGTCATAATGGTTGAGACC -3'

Sequencing Primer
(F):5'- ACTCTGGCTTACCTGGGAGTC -3'
(R):5'- ATCCCTGTTGTGAGTAAGT -3'

Posted On

2019-09-13