Mutagenetix > Incidental Mutation

Incidental Mutation 'R0647:Wdfy4'

57293

Institutional Source

Beutler Lab

Gene Symbol

Wdfy4

Ensembl Gene

ENSMUSG00000051506

Gene Name

WD repeat and FYVE domain containing 4

Synonyms

MMRRC Submission

038832-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R0647 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

32681504-32907465 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 32831656 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Cysteine to Arginine at position 857 (C857R)

Ref Sequence

ENSEMBL: ENSMUSP00000057556 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000061753] [ENSMUST00000130509]

AlphaFold

no structure available at present

Predicted Effect

possibly damaging
Transcript: ENSMUST00000061753
AA Change: C857R

PolyPhen 2 Score 0.956 (Sensitivity: 0.79; Specificity: 0.95)

SMART Domains

Protein: ENSMUSP00000057556
Gene: ENSMUSG00000051506
AA Change: C857R

Domain	Start	End	E-Value	Type
low complexity region	69	77	N/A	INTRINSIC
low complexity region	204	222	N/A	INTRINSIC
low complexity region	508	522	N/A	INTRINSIC
low complexity region	618	632	N/A	INTRINSIC
low complexity region	644	661	N/A	INTRINSIC
low complexity region	1585	1604	N/A	INTRINSIC
low complexity region	1899	1909	N/A	INTRINSIC
Pfam:PH_BEACH	2237	2348	1.2e-9	PFAM
Beach	2378	2660	3.69e-196	SMART
WD40	2761	2801	1.98e1	SMART
WD40	2811	2850	5.18e-7	SMART
WD40	2853	2891	9.94e-1	SMART
WD40	2893	2940	3.17e-2	SMART
WD40	2986	3021	3.31e0	SMART

Predicted Effect

possibly damaging
Transcript: ENSMUST00000130509
AA Change: C857R

PolyPhen 2 Score 0.911 (Sensitivity: 0.81; Specificity: 0.94)

SMART Domains

Protein: ENSMUSP00000117068
Gene: ENSMUSG00000051506
AA Change: C857R

Domain	Start	End	E-Value	Type
low complexity region	69	77	N/A	INTRINSIC
low complexity region	204	222	N/A	INTRINSIC
low complexity region	508	522	N/A	INTRINSIC
low complexity region	618	632	N/A	INTRINSIC
low complexity region	644	661	N/A	INTRINSIC
low complexity region	1596	1615	N/A	INTRINSIC
low complexity region	1795	1819	N/A	INTRINSIC
low complexity region	2019	2029	N/A	INTRINSIC
Pfam:PH_BEACH	2362	2473	1.2e-9	PFAM
Beach	2503	2785	3.69e-196	SMART
WD40	2886	2926	1.98e1	SMART
WD40	2936	2975	5.18e-7	SMART
WD40	2978	3016	9.94e-1	SMART
WD40	3018	3065	3.17e-2	SMART
WD40	3111	3146	3.31e0	SMART

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.3%
20x: 95.1%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 63 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adamts2	C	A	11: 50,494,265 (GRCm39)	T113K	probably damaging	Het
Adgre1	T	A	17: 57,718,003 (GRCm39)	N338K	probably damaging	Het
Aggf1	A	T	13: 95,508,164 (GRCm39)		probably null	Het
Apc2	A	T	10: 80,140,762 (GRCm39)	I206F	probably damaging	Het
Carmil3	T	C	14: 55,739,892 (GRCm39)		probably null	Het
Ccdc110	A	C	8: 46,396,425 (GRCm39)	E772A	probably damaging	Het
Cdh23	A	G	10: 60,143,681 (GRCm39)	F2977L	probably damaging	Het
Cdh23	A	T	10: 60,159,153 (GRCm39)	Y2207*	probably null	Het
Cfap20dc	T	A	14: 8,536,655 (GRCm38)	D184V	possibly damaging	Het
Chd4	T	A	6: 125,086,086 (GRCm39)	N908K	probably damaging	Het
Chst9	A	G	18: 15,585,726 (GRCm39)	I279T	probably damaging	Het
Ctnna3	A	T	10: 63,656,203 (GRCm39)	N261I	probably benign	Het
Dlgap2	A	T	8: 14,777,591 (GRCm39)	S279C	possibly damaging	Het
Dock4	G	A	12: 40,760,883 (GRCm39)	E524K	probably damaging	Het
Fabp12	T	A	3: 10,311,096 (GRCm39)	N122I	possibly damaging	Het
Fam184b	T	C	5: 45,741,932 (GRCm39)	T100A	probably benign	Het
Fbxl5	T	C	5: 43,925,411 (GRCm39)	D176G	probably damaging	Het
Fcho1	C	T	8: 72,165,204 (GRCm39)	A418T	probably benign	Het
Foxe1	A	G	4: 46,344,477 (GRCm39)	N95S	possibly damaging	Het
Frem3	A	G	8: 81,341,814 (GRCm39)	E1369G	probably damaging	Het
Frmpd4	C	T	X: 166,272,006 (GRCm39)	E483K	probably damaging	Het
Gbp11	T	C	5: 105,478,830 (GRCm39)	K203E	possibly damaging	Het
Hs3st6	C	T	17: 24,977,134 (GRCm39)	R205C	probably damaging	Het
Ifitm10	C	T	7: 141,909,772 (GRCm39)	S179N	probably damaging	Het
Irx2	A	C	13: 72,778,799 (GRCm39)	N121T	probably damaging	Het
Itih1	A	T	14: 30,657,820 (GRCm39)	V417E	probably damaging	Het
Itpr1	A	C	6: 108,360,659 (GRCm39)	E695A	probably damaging	Het
Kif1c	T	A	11: 70,616,967 (GRCm39)	I755K	probably damaging	Het
Lamb3	A	G	1: 193,013,104 (GRCm39)	E443G	probably damaging	Het
Lrp1	G	C	10: 127,407,346 (GRCm39)	T1865R	probably damaging	Het
Lrrc8b	T	A	5: 105,628,473 (GRCm39)	I273K	possibly damaging	Het
Ly9	T	C	1: 171,427,376 (GRCm39)	Y393C	probably damaging	Het
Mphosph8	T	C	14: 56,911,862 (GRCm39)	V295A	probably benign	Het
Nlrp5	T	A	7: 23,117,132 (GRCm39)	D269E	probably damaging	Het
Or1r1	G	A	11: 73,874,597 (GRCm39)	A279V	probably damaging	Het
Or2t6	T	G	14: 14,175,858 (GRCm38)	T75P	probably benign	Het
Or4k44	A	G	2: 111,367,704 (GRCm39)	V310A	probably benign	Het
Or52u1	T	G	7: 104,237,322 (GRCm39)	F104V	probably damaging	Het
Or5p50	T	A	7: 107,422,218 (GRCm39)	I153F	probably benign	Het
Otud3	A	G	4: 138,640,948 (GRCm39)	L64P	probably damaging	Het
Pcdh17	A	T	14: 84,685,213 (GRCm39)	H560L	possibly damaging	Het
Pcdhb21	T	C	18: 37,646,913 (GRCm39)	V14A	probably damaging	Het
Prss3l	A	T	6: 41,420,275 (GRCm39)	F150L	probably benign	Het
Rbfox1	A	T	16: 7,042,248 (GRCm39)	Q14L	probably damaging	Het
Rbm44	A	G	1: 91,084,650 (GRCm39)	D665G	probably benign	Het
Rc3h2	C	T	2: 37,299,542 (GRCm39)	V163M	probably damaging	Het
Sash1	T	A	10: 8,605,316 (GRCm39)	R1025W	probably damaging	Het
Sgpl1	A	G	10: 60,949,267 (GRCm39)	S146P	probably damaging	Het
Slc27a2	G	A	2: 126,429,836 (GRCm39)	D615N	probably benign	Het
Smap1	A	G	1: 23,892,559 (GRCm39)	I135T	probably damaging	Het
Snapc3	A	G	4: 83,368,466 (GRCm39)	D321G	probably damaging	Het
St6galnac4	C	T	2: 32,479,460 (GRCm39)	R6C	probably damaging	Het
Syne2	C	T	12: 75,934,977 (GRCm39)	P153L	probably benign	Het
Tiprl	A	G	1: 165,050,092 (GRCm39)		probably null	Het
Tmem94	A	G	11: 115,687,621 (GRCm39)	N1160S	probably damaging	Het
Trim65	G	A	11: 116,019,036 (GRCm39)	R168C	possibly damaging	Het
Txndc16	A	T	14: 45,406,732 (GRCm39)	I241N	probably damaging	Het
Txndc16	T	A	14: 45,402,818 (GRCm39)	R101*	probably null	Het
Ugt2b38	A	G	5: 87,571,328 (GRCm39)	S235P	probably benign	Het
Ugt3a1	A	T	15: 9,310,635 (GRCm39)	M306L	probably benign	Het
Vmn1r23	T	A	6: 57,903,169 (GRCm39)	Y203F	probably benign	Het
Vmn2r3	T	A	3: 64,183,046 (GRCm39)	I218F	probably damaging	Het
Zfp493	A	C	13: 67,931,994 (GRCm39)	K31T	possibly damaging	Het

Other mutations in Wdfy4

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00231:Wdfy4	APN	14	32,824,496 (GRCm39)	missense	possibly damaging	0.93
IGL01116:Wdfy4	APN	14	32,681,934 (GRCm39)	missense	probably damaging	1.00
IGL01449:Wdfy4	APN	14	32,825,994 (GRCm39)	missense	probably damaging	0.99
IGL01567:Wdfy4	APN	14	32,873,618 (GRCm39)	missense	probably benign	0.01
IGL01700:Wdfy4	APN	14	32,742,195 (GRCm39)	splice site	probably benign
IGL01931:Wdfy4	APN	14	32,877,710 (GRCm39)	missense	probably damaging	1.00
IGL01981:Wdfy4	APN	14	32,855,673 (GRCm39)	missense	probably damaging	1.00
IGL01988:Wdfy4	APN	14	32,798,437 (GRCm39)	missense	possibly damaging	0.75
IGL02026:Wdfy4	APN	14	32,815,257 (GRCm39)	missense	probably damaging	1.00
IGL02066:Wdfy4	APN	14	32,871,523 (GRCm39)	missense	probably benign
IGL02468:Wdfy4	APN	14	32,688,389 (GRCm39)	missense	probably benign	0.01
IGL02512:Wdfy4	APN	14	32,764,448 (GRCm39)	missense	probably benign	0.01
IGL02597:Wdfy4	APN	14	32,812,818 (GRCm39)	nonsense	probably null
IGL02752:Wdfy4	APN	14	32,798,283 (GRCm39)	missense	probably damaging	1.00
IGL02792:Wdfy4	APN	14	32,817,262 (GRCm39)	missense	probably benign	0.01
IGL02826:Wdfy4	APN	14	32,693,707 (GRCm39)	missense	possibly damaging	0.47
IGL02903:Wdfy4	APN	14	32,831,607 (GRCm39)	missense	probably damaging	1.00
IGL02955:Wdfy4	APN	14	32,798,241 (GRCm39)	missense	probably damaging	1.00
IGL03031:Wdfy4	APN	14	32,862,608 (GRCm39)	missense	probably damaging	1.00
IGL03102:Wdfy4	APN	14	32,688,392 (GRCm39)	missense	probably damaging	1.00
IGL03123:Wdfy4	APN	14	32,884,827 (GRCm39)	missense	probably benign	0.01
IGL03198:Wdfy4	APN	14	32,847,844 (GRCm39)	missense	probably damaging	1.00
IGL03250:Wdfy4	APN	14	32,699,124 (GRCm39)	missense	probably damaging	0.99
IGL03277:Wdfy4	APN	14	32,790,861 (GRCm39)	missense	probably benign	0.01
IGL03398:Wdfy4	APN	14	32,769,247 (GRCm39)	missense	probably benign	0.14
dodgers	UTSW	14	32,699,063 (GRCm39)	nonsense	probably null
Dollar	UTSW	14	32,742,268 (GRCm39)	missense	probably damaging	1.00
Giants	UTSW	14	32,792,575 (GRCm39)	nonsense	probably null
gigantea	UTSW	14	32,696,111 (GRCm39)	critical splice donor site	probably null
kings_canyon	UTSW	14	32,831,476 (GRCm39)	nonsense	probably null
moro	UTSW	14	32,686,583 (GRCm39)	splice site	probably null
popped	UTSW	14	32,688,356 (GRCm39)	missense	probably damaging	0.99
sequoia	UTSW	14	32,822,860 (GRCm39)	critical splice donor site	probably null
Sherman	UTSW	14	32,817,908 (GRCm39)	missense	possibly damaging	0.89
stretched	UTSW	14	32,795,492 (GRCm39)	nonsense	probably null
watchtower	UTSW	14	32,805,596 (GRCm39)	critical splice donor site	probably null
R0014:Wdfy4	UTSW	14	32,829,130 (GRCm39)	missense	possibly damaging	0.72
R0067:Wdfy4	UTSW	14	32,884,708 (GRCm39)	missense	probably null	1.00
R0085:Wdfy4	UTSW	14	32,800,200 (GRCm39)	missense	possibly damaging	0.81
R0277:Wdfy4	UTSW	14	32,805,742 (GRCm39)	missense	possibly damaging	0.83
R0436:Wdfy4	UTSW	14	32,805,769 (GRCm39)	splice site	probably benign
R0496:Wdfy4	UTSW	14	32,862,695 (GRCm39)	splice site	probably benign
R0514:Wdfy4	UTSW	14	32,802,732 (GRCm39)	missense	probably benign	0.22
R0548:Wdfy4	UTSW	14	32,764,578 (GRCm39)	missense	probably benign
R0590:Wdfy4	UTSW	14	32,763,131 (GRCm39)	missense	probably benign	0.09
R0766:Wdfy4	UTSW	14	32,862,569 (GRCm39)	missense	probably damaging	1.00
R0981:Wdfy4	UTSW	14	32,869,049 (GRCm39)	missense	probably benign	0.03
R1024:Wdfy4	UTSW	14	32,801,923 (GRCm39)	missense	possibly damaging	0.81
R1113:Wdfy4	UTSW	14	32,693,695 (GRCm39)	missense	possibly damaging	0.47
R1252:Wdfy4	UTSW	14	32,693,729 (GRCm39)	splice site	probably null
R1415:Wdfy4	UTSW	14	32,763,137 (GRCm39)	missense	possibly damaging	0.60
R1475:Wdfy4	UTSW	14	32,830,645 (GRCm39)	missense	probably benign	0.14
R1483:Wdfy4	UTSW	14	32,822,923 (GRCm39)	missense	probably benign	0.41
R1490:Wdfy4	UTSW	14	32,874,495 (GRCm39)	critical splice donor site	probably null
R1512:Wdfy4	UTSW	14	32,682,765 (GRCm39)	missense	probably damaging	0.98
R1615:Wdfy4	UTSW	14	32,764,469 (GRCm39)	missense	probably damaging	1.00
R1628:Wdfy4	UTSW	14	32,681,918 (GRCm39)	missense	probably damaging	1.00
R1643:Wdfy4	UTSW	14	32,795,542 (GRCm39)	critical splice acceptor site	probably null
R1729:Wdfy4	UTSW	14	32,817,962 (GRCm39)	missense	possibly damaging	0.85
R1859:Wdfy4	UTSW	14	32,825,940 (GRCm39)	missense	probably damaging	0.99
R1933:Wdfy4	UTSW	14	32,855,301 (GRCm39)	missense	probably benign	0.08
R1957:Wdfy4	UTSW	14	32,693,641 (GRCm39)	missense	probably damaging	1.00
R1968:Wdfy4	UTSW	14	32,828,001 (GRCm39)	missense	possibly damaging	0.95
R2032:Wdfy4	UTSW	14	32,868,946 (GRCm39)	missense	probably benign	0.11
R2241:Wdfy4	UTSW	14	32,795,468 (GRCm39)	missense	possibly damaging	0.81
R2391:Wdfy4	UTSW	14	32,884,764 (GRCm39)	missense	possibly damaging	0.92
R2888:Wdfy4	UTSW	14	32,831,476 (GRCm39)	nonsense	probably null
R2889:Wdfy4	UTSW	14	32,831,476 (GRCm39)	nonsense	probably null
R3114:Wdfy4	UTSW	14	32,811,860 (GRCm39)	missense	probably damaging	0.97
R3757:Wdfy4	UTSW	14	32,745,331 (GRCm39)	missense	probably benign	0.17
R3758:Wdfy4	UTSW	14	32,745,331 (GRCm39)	missense	probably benign	0.17
R3797:Wdfy4	UTSW	14	32,862,602 (GRCm39)	missense	probably damaging	1.00
R3890:Wdfy4	UTSW	14	32,769,237 (GRCm39)	missense	probably damaging	1.00
R3892:Wdfy4	UTSW	14	32,769,237 (GRCm39)	missense	probably damaging	1.00
R3945:Wdfy4	UTSW	14	32,688,352 (GRCm39)	missense	probably damaging	0.99
R4011:Wdfy4	UTSW	14	32,824,637 (GRCm39)	splice site	probably benign
R4091:Wdfy4	UTSW	14	32,847,837 (GRCm39)	missense	possibly damaging	0.93
R4449:Wdfy4	UTSW	14	32,818,040 (GRCm39)	missense	probably damaging	1.00
R4585:Wdfy4	UTSW	14	32,809,912 (GRCm39)	missense	possibly damaging	0.89
R4628:Wdfy4	UTSW	14	32,824,515 (GRCm39)	missense	probably damaging	0.97
R4629:Wdfy4	UTSW	14	32,824,515 (GRCm39)	missense	probably damaging	0.97
R4655:Wdfy4	UTSW	14	32,711,893 (GRCm39)	missense	probably damaging	0.98
R4689:Wdfy4	UTSW	14	32,831,505 (GRCm39)	missense	possibly damaging	0.88
R4718:Wdfy4	UTSW	14	32,867,273 (GRCm39)	missense	probably benign	0.03
R4862:Wdfy4	UTSW	14	32,822,860 (GRCm39)	critical splice donor site	probably null
R4884:Wdfy4	UTSW	14	32,710,852 (GRCm39)	nonsense	probably null
R4894:Wdfy4	UTSW	14	32,877,717 (GRCm39)	missense	probably benign	0.03
R4929:Wdfy4	UTSW	14	32,769,213 (GRCm39)	missense	possibly damaging	0.90
R4932:Wdfy4	UTSW	14	32,750,970 (GRCm39)	missense	probably damaging	1.00
R5014:Wdfy4	UTSW	14	32,822,897 (GRCm39)	missense	probably benign	0.02
R5020:Wdfy4	UTSW	14	32,801,892 (GRCm39)	missense	probably damaging	1.00
R5049:Wdfy4	UTSW	14	32,874,627 (GRCm39)	missense	possibly damaging	0.78
R5276:Wdfy4	UTSW	14	32,769,232 (GRCm39)	missense	probably damaging	1.00
R5318:Wdfy4	UTSW	14	32,800,300 (GRCm39)	missense	possibly damaging	0.95
R5338:Wdfy4	UTSW	14	32,812,823 (GRCm39)	missense	probably damaging	1.00
R5349:Wdfy4	UTSW	14	32,710,856 (GRCm39)	missense	probably damaging	1.00
R5411:Wdfy4	UTSW	14	32,681,959 (GRCm39)	missense	probably damaging	1.00
R5435:Wdfy4	UTSW	14	32,742,268 (GRCm39)	missense	probably damaging	1.00
R5463:Wdfy4	UTSW	14	32,873,689 (GRCm39)	missense	probably benign	0.17
R5591:Wdfy4	UTSW	14	32,829,087 (GRCm39)	missense	probably benign	0.09
R5598:Wdfy4	UTSW	14	32,855,454 (GRCm39)	missense	probably damaging	1.00
R5654:Wdfy4	UTSW	14	32,829,575 (GRCm39)	splice site	probably null
R5890:Wdfy4	UTSW	14	32,824,534 (GRCm39)	missense	possibly damaging	0.91
R5894:Wdfy4	UTSW	14	32,855,317 (GRCm39)	missense	possibly damaging	0.86
R5964:Wdfy4	UTSW	14	32,827,968 (GRCm39)	missense	probably damaging	1.00
R6036:Wdfy4	UTSW	14	32,868,947 (GRCm39)	missense	probably damaging	0.97
R6036:Wdfy4	UTSW	14	32,868,947 (GRCm39)	missense	probably damaging	0.97
R6074:Wdfy4	UTSW	14	32,805,596 (GRCm39)	critical splice donor site	probably null
R6135:Wdfy4	UTSW	14	32,693,668 (GRCm39)	missense	probably damaging	0.99
R6276:Wdfy4	UTSW	14	32,831,482 (GRCm39)	missense	possibly damaging	0.54
R6357:Wdfy4	UTSW	14	32,823,006 (GRCm39)	nonsense	probably null
R6370:Wdfy4	UTSW	14	32,790,807 (GRCm39)	missense	probably benign	0.16
R6390:Wdfy4	UTSW	14	32,826,051 (GRCm39)	missense	probably damaging	0.99
R6413:Wdfy4	UTSW	14	32,689,604 (GRCm39)	missense	probably damaging	1.00
R6450:Wdfy4	UTSW	14	32,830,649 (GRCm39)	missense	probably damaging	1.00
R6522:Wdfy4	UTSW	14	32,868,901 (GRCm39)	missense	probably damaging	0.98
R6657:Wdfy4	UTSW	14	32,769,208 (GRCm39)	missense	possibly damaging	0.70
R6761:Wdfy4	UTSW	14	32,817,908 (GRCm39)	missense	possibly damaging	0.89
R6763:Wdfy4	UTSW	14	32,764,469 (GRCm39)	missense	probably damaging	1.00
R6952:Wdfy4	UTSW	14	32,681,923 (GRCm39)	missense	probably damaging	1.00
R6985:Wdfy4	UTSW	14	32,821,074 (GRCm39)	missense	possibly damaging	0.68
R7024:Wdfy4	UTSW	14	32,686,583 (GRCm39)	splice site	probably null
R7101:Wdfy4	UTSW	14	32,682,777 (GRCm39)	missense
R7114:Wdfy4	UTSW	14	32,693,531 (GRCm39)	splice site	probably null
R7139:Wdfy4	UTSW	14	32,873,535 (GRCm39)	missense
R7255:Wdfy4	UTSW	14	32,696,239 (GRCm39)	missense
R7324:Wdfy4	UTSW	14	32,769,271 (GRCm39)	missense
R7379:Wdfy4	UTSW	14	32,873,566 (GRCm39)	missense
R7399:Wdfy4	UTSW	14	32,790,863 (GRCm39)	missense
R7408:Wdfy4	UTSW	14	32,800,264 (GRCm39)	missense
R7410:Wdfy4	UTSW	14	32,696,191 (GRCm39)	missense
R7411:Wdfy4	UTSW	14	32,828,088 (GRCm39)	missense
R7412:Wdfy4	UTSW	14	32,871,541 (GRCm39)	missense
R7445:Wdfy4	UTSW	14	32,792,575 (GRCm39)	nonsense	probably null
R7595:Wdfy4	UTSW	14	32,696,111 (GRCm39)	critical splice donor site	probably null
R7618:Wdfy4	UTSW	14	32,707,696 (GRCm39)	missense
R7622:Wdfy4	UTSW	14	32,800,231 (GRCm39)	missense
R7828:Wdfy4	UTSW	14	32,710,878 (GRCm39)	missense	possibly damaging	0.90
R7888:Wdfy4	UTSW	14	32,812,920 (GRCm39)	missense
R7946:Wdfy4	UTSW	14	32,826,072 (GRCm39)	missense
R7946:Wdfy4	UTSW	14	32,792,705 (GRCm39)	missense
R7986:Wdfy4	UTSW	14	32,826,072 (GRCm39)	missense
R7990:Wdfy4	UTSW	14	32,819,752 (GRCm39)	missense
R8001:Wdfy4	UTSW	14	32,695,492 (GRCm39)	critical splice donor site	probably null
R8010:Wdfy4	UTSW	14	32,693,584 (GRCm39)	missense
R8015:Wdfy4	UTSW	14	32,829,704 (GRCm39)	missense
R8032:Wdfy4	UTSW	14	32,751,043 (GRCm39)	nonsense	probably null
R8041:Wdfy4	UTSW	14	32,875,965 (GRCm39)	critical splice donor site	probably null
R8090:Wdfy4	UTSW	14	32,826,072 (GRCm39)	missense
R8092:Wdfy4	UTSW	14	32,826,072 (GRCm39)	missense
R8112:Wdfy4	UTSW	14	32,826,072 (GRCm39)	missense
R8114:Wdfy4	UTSW	14	32,826,072 (GRCm39)	missense
R8115:Wdfy4	UTSW	14	32,826,072 (GRCm39)	missense
R8117:Wdfy4	UTSW	14	32,826,072 (GRCm39)	missense
R8117:Wdfy4	UTSW	14	32,699,063 (GRCm39)	nonsense	probably null
R8118:Wdfy4	UTSW	14	32,826,072 (GRCm39)	missense
R8140:Wdfy4	UTSW	14	32,864,317 (GRCm39)	missense
R8155:Wdfy4	UTSW	14	32,884,776 (GRCm39)	missense
R8163:Wdfy4	UTSW	14	32,873,545 (GRCm39)	missense
R8293:Wdfy4	UTSW	14	32,696,218 (GRCm39)	missense
R8325:Wdfy4	UTSW	14	32,689,444 (GRCm39)	missense
R8353:Wdfy4	UTSW	14	32,695,581 (GRCm39)	missense	probably benign
R8370:Wdfy4	UTSW	14	32,815,208 (GRCm39)	missense
R8437:Wdfy4	UTSW	14	32,798,332 (GRCm39)	missense
R8497:Wdfy4	UTSW	14	32,688,356 (GRCm39)	missense	probably damaging	0.99
R8545:Wdfy4	UTSW	14	32,800,258 (GRCm39)	missense	probably benign	0.01
R8671:Wdfy4	UTSW	14	32,693,722 (GRCm39)	splice site	probably benign
R8708:Wdfy4	UTSW	14	32,689,489 (GRCm39)	missense
R8747:Wdfy4	UTSW	14	32,874,611 (GRCm39)	missense
R8794:Wdfy4	UTSW	14	32,869,049 (GRCm39)	missense	probably benign	0.03
R8846:Wdfy4	UTSW	14	32,867,105 (GRCm39)	missense
R8880:Wdfy4	UTSW	14	32,795,492 (GRCm39)	nonsense	probably null
R9109:Wdfy4	UTSW	14	32,760,704 (GRCm39)	splice site	probably null
R9131:Wdfy4	UTSW	14	32,819,807 (GRCm39)	missense
R9309:Wdfy4	UTSW	14	32,817,313 (GRCm39)	missense
R9349:Wdfy4	UTSW	14	32,875,996 (GRCm39)	missense
R9451:Wdfy4	UTSW	14	32,855,518 (GRCm39)	missense
R9563:Wdfy4	UTSW	14	32,692,833 (GRCm39)	missense
R9587:Wdfy4	UTSW	14	32,769,230 (GRCm39)	nonsense	probably null
R9599:Wdfy4	UTSW	14	32,855,428 (GRCm39)	missense
R9670:Wdfy4	UTSW	14	32,769,219 (GRCm39)	missense
R9718:Wdfy4	UTSW	14	32,847,893 (GRCm39)	missense
R9742:Wdfy4	UTSW	14	32,809,987 (GRCm39)	missense
X0028:Wdfy4	UTSW	14	32,802,593 (GRCm39)	missense	probably benign
X0053:Wdfy4	UTSW	14	32,884,899 (GRCm39)	start codon destroyed	probably null	0.99
X0062:Wdfy4	UTSW	14	32,829,575 (GRCm39)	splice site	probably null
Z1177:Wdfy4	UTSW	14	32,809,942 (GRCm39)	missense

Predicted Primers

PCR Primer
(F):5'- GCAGTACCTTAGCACATCTGGCTC -3'
(R):5'- GCGACCAATTCAGAAAGTGTCCCC -3'

Sequencing Primer
(F):5'- AGCACATCTGGCTCTATGGC -3'
(R):5'- GGTTCCAACATCACATTTCTGAGG -3'

Posted On

2013-07-11