Mutagenetix > Incidental Mutation

Incidental Mutation 'R7385:Slitrk5'

573056

Institutional Source

Beutler Lab

Gene Symbol

Slitrk5

Ensembl Gene

ENSMUSG00000033214

Gene Name

SLIT and NTRK-like family, member 5

Synonyms

2610019D03Rik

MMRRC Submission

045467-MU

Accession Numbers

Essential gene?

Possibly non essential (E-score: 0.451) question?

Stock #

R7385 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

111912547-111920576 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 111918131 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Valine to Glutamic Acid at position 585 (V585E)

Ref Sequence

ENSEMBL: ENSMUSP00000041499 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000042767] [ENSMUST00000227891]

AlphaFold

Q810B7

Predicted Effect

probably benign
Transcript: ENSMUST00000042767
AA Change: V585E

PolyPhen 2 Score 0.317 (Sensitivity: 0.90; Specificity: 0.89)

SMART Domains

Protein: ENSMUSP00000041499
Gene: ENSMUSG00000033214
AA Change: V585E

Domain	Start	End	E-Value	Type
Blast:LRRNT	47	85	3e-18	BLAST
low complexity region	86	96	N/A	INTRINSIC
LRR	108	127	2.76e2	SMART
LRR_TYP	128	151	1.67e-2	SMART
LRR	152	175	2.67e-1	SMART
LRR	176	199	1.08e-1	SMART
LRR	202	223	7.38e1	SMART
LRRCT	235	285	2.13e-5	SMART
low complexity region	308	323	N/A	INTRINSIC
LRRNT	373	410	9.53e-2	SMART
LRR	433	455	1.45e1	SMART
LRR_TYP	456	479	4.94e-5	SMART
LRR_TYP	480	503	7.78e-3	SMART
LRR_TYP	504	527	2.43e-4	SMART
LRR	528	551	1.86e2	SMART
LRRCT	563	613	3.59e-3	SMART
low complexity region	618	632	N/A	INTRINSIC
transmembrane domain	666	688	N/A	INTRINSIC
low complexity region	794	816	N/A	INTRINSIC
low complexity region	818	823	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000227891

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.7%
20x: 99.0%

Validation Efficiency

100% (75/75)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] Members of the SLITRK family, such as SLITRK5, are integral membrane proteins with 2 N-terminal leucine-rich repeat (LRR) domains similar to those of SLIT proteins (see SLIT1; MIM 603742). Most SLITRKs, including SLITRK5, also have C-terminal regions that share homology with neurotrophin receptors (see NTRK1; MIM 191315). SLITRKs are expressed predominantly in neural tissues and have neurite-modulating activity (Aruga et al., 2003 [PubMed 14557068]).[supplied by OMIM, Mar 2008]
PHENOTYPE: Mice homozygous for a null allele have abnormal medium spiny neuron morphology and exhibit behavioral abnormalities. [provided by MGI curators]

Allele List at MGI

All alleles(3) : Targeted(3)

Other mutations in this stock

Total: 74 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Ahctf1	T	C	1: 179,580,946 (GRCm39)	E1752G	possibly damaging	Het
Aqp3	T	C	4: 41,095,178 (GRCm39)	T68A	probably damaging	Het
Arhgap40	A	G	2: 158,385,147 (GRCm39)	K463R	probably damaging	Het
Asb10	A	T	5: 24,738,736 (GRCm39)	C440*	probably null	Het
Bach1	A	G	16: 87,526,385 (GRCm39)	T616A	probably damaging	Het
Braf	T	A	6: 39,642,042 (GRCm39)		probably null	Het
Cacna1s	A	T	1: 136,020,371 (GRCm39)	N803Y	probably damaging	Het
Cald1	A	G	6: 34,663,000 (GRCm39)	E21G	probably damaging	Het
Caskin1	G	T	17: 24,722,898 (GRCm39)	G589C	probably damaging	Het
Cdc37l1	T	C	19: 28,968,071 (GRCm39)		probably null	Het
Cntnap5b	G	A	1: 100,306,815 (GRCm39)	G844D	probably damaging	Het
Col5a1	T	C	2: 27,914,762 (GRCm39)	L1615P	unknown	Het
Cpt1a	C	A	19: 3,430,155 (GRCm39)	P672T	probably damaging	Het
Defb22	A	G	2: 152,328,117 (GRCm39)	Y23H	probably damaging	Het
Depdc1b	G	C	13: 108,500,166 (GRCm39)	K226N	probably damaging	Het
Derl2	A	G	11: 70,909,764 (GRCm39)		probably benign	Het
Dnaja2	T	C	8: 86,265,982 (GRCm39)	T368A	probably benign	Het
Dsg3	C	A	18: 20,673,254 (GRCm39)	T975K	possibly damaging	Het
Eif4enif1	T	A	11: 3,170,269 (GRCm39)	D107E	probably damaging	Het
Fut11	A	G	14: 20,746,325 (GRCm39)	D389G	probably damaging	Het
Gopc	C	T	10: 52,225,328 (GRCm39)	G299E	probably damaging	Het
Gprin1	T	C	13: 54,886,423 (GRCm39)	D617G	probably benign	Het
Grin2d	A	G	7: 45,506,960 (GRCm39)	V505A	probably damaging	Het
Heatr6	T	C	11: 83,650,161 (GRCm39)	Y206H	probably damaging	Het
Hhex	C	A	19: 37,425,713 (GRCm39)	N147K	probably damaging	Het
Igkv4-80	G	A	6: 68,993,699 (GRCm39)	S64F	probably damaging	Het
Jakmip3	A	G	7: 138,625,068 (GRCm39)	K360R	possibly damaging	Het
Kat5	AG	A	19: 5,658,297 (GRCm39)		probably null	Het
Kat5	T	A	19: 5,658,302 (GRCm39)	N191I	probably benign	Het
Kifc5b	A	G	17: 27,144,597 (GRCm39)	D572G	probably damaging	Het
Lrp5	C	A	19: 3,662,197 (GRCm39)		probably null	Het
Lrtm1	A	G	14: 28,749,673 (GRCm39)	M345V	probably benign	Het
Mbd5	T	C	2: 49,162,461 (GRCm39)	V981A	probably benign	Het
Mier3	G	A	13: 111,841,783 (GRCm39)	G115S	possibly damaging	Het
Mrgprd	A	G	7: 144,875,261 (GRCm39)	N44S	probably damaging	Het
Mrps10	C	A	17: 47,689,146 (GRCm39)	P181Q	probably damaging	Het
Myot	T	A	18: 44,470,075 (GRCm39)	C17*	probably null	Het
Myt1	A	G	2: 181,409,498 (GRCm39)		probably null	Het
Ncoa6	C	A	2: 155,249,721 (GRCm39)	L1194F	probably damaging	Het
Or4d11	T	C	19: 12,013,363 (GRCm39)	T248A	probably benign	Het
Or4d6	T	C	19: 12,086,061 (GRCm39)	N57S	probably damaging	Het
Or4e5	T	C	14: 52,727,638 (GRCm39)	Y261C	probably damaging	Het
Or7a39	C	A	10: 78,715,288 (GRCm39)	T94K	probably damaging	Het
Or7e165	T	A	9: 19,694,507 (GRCm39)	I26N	possibly damaging	Het
Osbpl6	G	A	2: 76,379,794 (GRCm39)	G128E	probably damaging	Het
P3h3	A	T	6: 124,832,233 (GRCm39)	Y218N	probably damaging	Het
Paxip1	A	G	5: 27,986,418 (GRCm39)		probably null	Het
Pdia5	T	C	16: 35,250,284 (GRCm39)	Y225C	probably damaging	Het
Pik3c2g	T	G	6: 139,801,079 (GRCm39)	M526R		Het
Prox1	A	G	1: 189,894,323 (GRCm39)	F41L	probably benign	Het
Psd3	A	T	8: 68,453,408 (GRCm39)	F284I	probably damaging	Het
Rev3l	T	A	10: 39,699,678 (GRCm39)	C1392S	probably benign	Het
Rfxank	A	T	8: 70,587,285 (GRCm39)	V212E	probably damaging	Het
Ros1	T	C	10: 52,031,222 (GRCm39)	D482G	probably benign	Het
Sat2	A	T	11: 69,513,763 (GRCm39)	I94F	probably damaging	Het
Scarf2	T	C	16: 17,621,702 (GRCm39)	L384P	probably damaging	Het
Sec14l2	C	A	11: 4,066,750 (GRCm39)	E21*	probably null	Het
Spata31h1	T	A	10: 82,123,571 (GRCm39)	E3146D	probably benign	Het
Spata31h1	T	C	10: 82,123,729 (GRCm39)	S3094G	probably benign	Het
Tas2r130	T	A	6: 131,607,226 (GRCm39)	M190L	probably benign	Het
Ticrr	G	A	7: 79,341,597 (GRCm39)	S1061N	possibly damaging	Het
Tlr9	C	A	9: 106,102,463 (GRCm39)	H585N	probably damaging	Het
Tmem219	A	T	7: 126,495,947 (GRCm39)	I142N	probably damaging	Het
Tnni2	A	G	7: 141,996,915 (GRCm39)	N8S	probably benign	Het
Tnpo2	G	A	8: 85,776,748 (GRCm39)	R485H	probably damaging	Het
Ttc8	A	G	12: 98,908,547 (GRCm39)	E72G	possibly damaging	Het
Upf1	A	G	8: 70,793,268 (GRCm39)	Y297H	probably damaging	Het
Vmn1r4	A	G	6: 56,933,721 (GRCm39)	K75R	probably damaging	Het
Vmn2r78	G	A	7: 86,571,633 (GRCm39)	G481D	probably benign	Het
Vmn2r96	A	G	17: 18,803,302 (GRCm39)	Y404C	probably damaging	Het
Vps50	T	A	6: 3,602,708 (GRCm39)	S942T	probably benign	Het
Xkr7	C	A	2: 152,895,983 (GRCm39)	S279*	probably null	Het
Zan	G	A	5: 137,432,416 (GRCm39)	Q2294*	probably null	Het
Zan	T	C	5: 137,448,753 (GRCm39)	Y1700C	unknown	Het

Other mutations in Slitrk5

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00801:Slitrk5	APN	14	111,918,097 (GRCm39)	missense	probably benign	0.05
IGL01624:Slitrk5	APN	14	111,918,526 (GRCm39)	missense	probably damaging	1.00
IGL01680:Slitrk5	APN	14	111,916,432 (GRCm39)	missense	probably benign	0.23
IGL03234:Slitrk5	APN	14	111,916,717 (GRCm39)	missense	probably benign	0.00
P0019:Slitrk5	UTSW	14	111,918,026 (GRCm39)	missense	possibly damaging	0.88
R0323:Slitrk5	UTSW	14	111,919,055 (GRCm39)	missense	probably damaging	0.99
R0334:Slitrk5	UTSW	14	111,918,256 (GRCm39)	missense	probably benign
R0392:Slitrk5	UTSW	14	111,916,465 (GRCm39)	missense	probably benign	0.06
R0659:Slitrk5	UTSW	14	111,918,121 (GRCm39)	missense	probably benign	0.00
R1344:Slitrk5	UTSW	14	111,917,821 (GRCm39)	missense	probably benign	0.04
R1754:Slitrk5	UTSW	14	111,917,951 (GRCm39)	missense	probably damaging	1.00
R1983:Slitrk5	UTSW	14	111,917,821 (GRCm39)	missense	probably benign	0.04
R2070:Slitrk5	UTSW	14	111,917,621 (GRCm39)	missense	probably damaging	0.99
R2071:Slitrk5	UTSW	14	111,917,621 (GRCm39)	missense	probably damaging	0.99
R3001:Slitrk5	UTSW	14	111,917,014 (GRCm39)	missense	probably damaging	1.00
R3002:Slitrk5	UTSW	14	111,917,014 (GRCm39)	missense	probably damaging	1.00
R3003:Slitrk5	UTSW	14	111,917,014 (GRCm39)	missense	probably damaging	1.00
R3885:Slitrk5	UTSW	14	111,917,229 (GRCm39)	nonsense	probably null
R3886:Slitrk5	UTSW	14	111,917,229 (GRCm39)	nonsense	probably null
R3888:Slitrk5	UTSW	14	111,917,229 (GRCm39)	nonsense	probably null
R4962:Slitrk5	UTSW	14	111,918,679 (GRCm39)	missense	probably benign	0.02
R4999:Slitrk5	UTSW	14	111,917,648 (GRCm39)	missense	probably damaging	0.99
R5036:Slitrk5	UTSW	14	111,918,316 (GRCm39)	missense	possibly damaging	0.87
R5190:Slitrk5	UTSW	14	111,916,852 (GRCm39)	missense	probably damaging	1.00
R5237:Slitrk5	UTSW	14	111,919,118 (GRCm39)	missense	possibly damaging	0.94
R5669:Slitrk5	UTSW	14	111,919,055 (GRCm39)	missense	probably damaging	0.99
R5793:Slitrk5	UTSW	14	111,917,345 (GRCm39)	missense	probably damaging	1.00
R5839:Slitrk5	UTSW	14	111,917,030 (GRCm39)	missense	probably benign	0.00
R6083:Slitrk5	UTSW	14	111,919,157 (GRCm39)	missense	probably benign	0.01
R6224:Slitrk5	UTSW	14	111,917,248 (GRCm39)	unclassified	probably benign
R6225:Slitrk5	UTSW	14	111,917,248 (GRCm39)	unclassified	probably benign
R6230:Slitrk5	UTSW	14	111,917,248 (GRCm39)	unclassified	probably benign
R6337:Slitrk5	UTSW	14	111,917,684 (GRCm39)	missense	probably damaging	0.96
R6666:Slitrk5	UTSW	14	111,917,534 (GRCm39)	missense	probably damaging	0.96
R6818:Slitrk5	UTSW	14	111,917,726 (GRCm39)	missense	probably benign	0.32
R6895:Slitrk5	UTSW	14	111,919,085 (GRCm39)	missense	probably damaging	1.00
R7094:Slitrk5	UTSW	14	111,918,268 (GRCm39)	missense	probably benign	0.02
R8532:Slitrk5	UTSW	14	111,916,909 (GRCm39)	missense	probably benign
R8994:Slitrk5	UTSW	14	111,918,227 (GRCm39)	missense	probably benign	0.00
R9344:Slitrk5	UTSW	14	111,916,702 (GRCm39)	missense	probably damaging	0.97
R9374:Slitrk5	UTSW	14	111,916,496 (GRCm39)	missense	probably benign	0.23
R9499:Slitrk5	UTSW	14	111,916,496 (GRCm39)	missense	probably benign	0.23
R9500:Slitrk5	UTSW	14	111,916,726 (GRCm39)	missense	possibly damaging	0.89
R9512:Slitrk5	UTSW	14	111,917,252 (GRCm39)	missense	probably damaging	1.00
R9552:Slitrk5	UTSW	14	111,916,496 (GRCm39)	missense	probably benign	0.23
Z1177:Slitrk5	UTSW	14	111,917,285 (GRCm39)	missense	probably benign	0.37

Predicted Primers

PCR Primer
(F):5'- CTGGGACTTTTGATCCCGTC -3'
(R):5'- TTCAACCTCACAGCTGGAGTC -3'

Sequencing Primer
(F):5'- TTCTTGAATAACAACCAACTGCAGG -3'
(R):5'- TCACAGCTGGAGTCGCTGC -3'

Posted On

2019-09-13