Mutagenetix > Incidental Mutation

Incidental Mutation 'R7396:Etl4'

573792

Institutional Source

Beutler Lab

Gene Symbol

Etl4

Ensembl Gene

ENSMUSG00000036617

Gene Name

enhancer trap locus 4

Synonyms

6620402G01Rik, Sickle tail, E330027G05Rik, Etl-4, Skt, 9430077C05Rik

MMRRC Submission

045478-MU

Accession Numbers

Essential gene?

Probably essential (E-score: 0.809) question?

Stock #

R7396 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

19915018-20815346 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

C to T at 20803449 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Proline to Leucine at position 1057 (P1057L)

Ref Sequence

ENSEMBL: ENSMUSP00000041431 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000045555] [ENSMUST00000066509] [ENSMUST00000114604] [ENSMUST00000114606] [ENSMUST00000114607] [ENSMUST00000114608] [ENSMUST00000114614] [ENSMUST00000114627]

AlphaFold

A2AQ25

Predicted Effect

possibly damaging
Transcript: ENSMUST00000045555
AA Change: P1057L

PolyPhen 2 Score 0.619 (Sensitivity: 0.87; Specificity: 0.91)

SMART Domains

Protein: ENSMUSP00000041431
Gene: ENSMUSG00000036617
AA Change: P1057L

Domain	Start	End	E-Value	Type
Pfam:AIP3	188	291	1.7e-11	PFAM
low complexity region	313	328	N/A	INTRINSIC
low complexity region	350	368	N/A	INTRINSIC
coiled coil region	620	652	N/A	INTRINSIC
low complexity region	1067	1096	N/A	INTRINSIC
low complexity region	1212	1231	N/A	INTRINSIC
low complexity region	1296	1314	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000066509
AA Change: P1092L

PolyPhen 2 Score 0.014 (Sensitivity: 0.96; Specificity: 0.79)

SMART Domains

Protein: ENSMUSP00000066170
Gene: ENSMUSG00000036617
AA Change: P1092L

Domain	Start	End	E-Value	Type
low complexity region	313	328	N/A	INTRINSIC
low complexity region	350	368	N/A	INTRINSIC
coiled coil region	655	687	N/A	INTRINSIC
low complexity region	1102	1131	N/A	INTRINSIC
low complexity region	1372	1381	N/A	INTRINSIC
low complexity region	1470	1495	N/A	INTRINSIC
low complexity region	1571	1582	N/A	INTRINSIC
coiled coil region	1658	1686	N/A	INTRINSIC
low complexity region	1724	1737	N/A	INTRINSIC
low complexity region	1806	1825	N/A	INTRINSIC
low complexity region	1890	1908	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000114604
AA Change: P1092L

PolyPhen 2 Score 0.967 (Sensitivity: 0.77; Specificity: 0.95)

SMART Domains

Protein: ENSMUSP00000110251
Gene: ENSMUSG00000036617
AA Change: P1092L

Domain	Start	End	E-Value	Type
Pfam:AIP3	188	291	1.7e-11	PFAM
low complexity region	313	328	N/A	INTRINSIC
low complexity region	350	368	N/A	INTRINSIC
coiled coil region	655	687	N/A	INTRINSIC
low complexity region	1102	1131	N/A	INTRINSIC
low complexity region	1207	1226	N/A	INTRINSIC
low complexity region	1291	1309	N/A	INTRINSIC

Predicted Effect

possibly damaging
Transcript: ENSMUST00000114606
AA Change: P775L

PolyPhen 2 Score 0.815 (Sensitivity: 0.84; Specificity: 0.93)

SMART Domains

Protein: ENSMUSP00000110253
Gene: ENSMUSG00000036617
AA Change: P775L

Domain	Start	End	E-Value	Type
low complexity region	31	46	N/A	INTRINSIC
low complexity region	68	86	N/A	INTRINSIC
coiled coil region	338	370	N/A	INTRINSIC
low complexity region	785	814	N/A	INTRINSIC

Predicted Effect

possibly damaging
Transcript: ENSMUST00000114607
AA Change: P775L

PolyPhen 2 Score 0.674 (Sensitivity: 0.86; Specificity: 0.92)

SMART Domains

Protein: ENSMUSP00000110254
Gene: ENSMUSG00000036617
AA Change: P775L

Domain	Start	End	E-Value	Type
low complexity region	31	46	N/A	INTRINSIC
low complexity region	68	86	N/A	INTRINSIC
coiled coil region	338	370	N/A	INTRINSIC
low complexity region	785	814	N/A	INTRINSIC

Predicted Effect

possibly damaging
Transcript: ENSMUST00000114608
AA Change: P775L

PolyPhen 2 Score 0.786 (Sensitivity: 0.85; Specificity: 0.93)

SMART Domains

Protein: ENSMUSP00000110255
Gene: ENSMUSG00000036617
AA Change: P775L

Domain	Start	End	E-Value	Type
low complexity region	31	46	N/A	INTRINSIC
low complexity region	68	86	N/A	INTRINSIC
coiled coil region	338	370	N/A	INTRINSIC
low complexity region	785	814	N/A	INTRINSIC
low complexity region	1055	1064	N/A	INTRINSIC
low complexity region	1153	1178	N/A	INTRINSIC
low complexity region	1254	1265	N/A	INTRINSIC
coiled coil region	1341	1369	N/A	INTRINSIC
low complexity region	1407	1420	N/A	INTRINSIC
low complexity region	1489	1508	N/A	INTRINSIC
low complexity region	1573	1591	N/A	INTRINSIC

Predicted Effect

possibly damaging
Transcript: ENSMUST00000114614
AA Change: P1046L

PolyPhen 2 Score 0.785 (Sensitivity: 0.85; Specificity: 0.93)

SMART Domains

Protein: ENSMUSP00000110261
Gene: ENSMUSG00000036617
AA Change: P1046L

Domain	Start	End	E-Value	Type
Pfam:AIP3	188	291	1.7e-11	PFAM
low complexity region	313	328	N/A	INTRINSIC
low complexity region	350	368	N/A	INTRINSIC
coiled coil region	620	652	N/A	INTRINSIC
low complexity region	1056	1085	N/A	INTRINSIC
low complexity region	1201	1220	N/A	INTRINSIC
low complexity region	1285	1303	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000114627
AA Change: P1143L

PolyPhen 2 Score 0.300 (Sensitivity: 0.90; Specificity: 0.89)

SMART Domains

Protein: ENSMUSP00000110274
Gene: ENSMUSG00000036617
AA Change: P1143L

Domain	Start	End	E-Value	Type
low complexity region	21	38	N/A	INTRINSIC
low complexity region	60	72	N/A	INTRINSIC
Pfam:AIP3	239	341	2.4e-14	PFAM
low complexity region	364	379	N/A	INTRINSIC
low complexity region	401	419	N/A	INTRINSIC
Pfam:AIP3	600	841	1.1e-12	PFAM
low complexity region	1153	1182	N/A	INTRINSIC
low complexity region	1423	1432	N/A	INTRINSIC
low complexity region	1521	1546	N/A	INTRINSIC
low complexity region	1622	1633	N/A	INTRINSIC
coiled coil region	1709	1737	N/A	INTRINSIC
low complexity region	1775	1788	N/A	INTRINSIC
low complexity region	1857	1876	N/A	INTRINSIC
low complexity region	1941	1959	N/A	INTRINSIC

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 98.9%

Validation Efficiency

100% (79/79)

MGI Phenotype

PHENOTYPE: Mice homozygous for a gene-trapped allele display malformations of the notochord and caudal vertebrae and may exhibit caudal tail kinks. Mice homozygous for another gene-trapped allele have malformed caudal vertebrae and intervertebral disk abnormalities; about half display kinked tails. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 81 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
3830403N18Rik	G	T	X: 55,184,140 (GRCm39)		probably null	Het
Abcb5	A	G	12: 118,831,609 (GRCm39)	Y1248H	probably damaging	Het
Acacb	A	G	5: 114,351,722 (GRCm39)	D1153G	possibly damaging	Het
Acsm3	T	A	7: 119,373,052 (GRCm39)	L185H	probably damaging	Het
Actr1a	G	A	19: 46,368,068 (GRCm39)	T293I	probably benign	Het
Adig	T	C	2: 158,347,836 (GRCm39)	L50P	unknown	Het
Ankrd28	A	G	14: 31,424,159 (GRCm39)	S994P	probably benign	Het
Ankrd35	T	A	3: 96,590,813 (GRCm39)	D366E	probably damaging	Het
Aqr	A	T	2: 113,950,427 (GRCm39)	Y927*	probably null	Het
Asxl2	T	A	12: 3,492,529 (GRCm39)	I38N	probably damaging	Het
Atp9b	T	C	18: 80,780,057 (GRCm39)	I1092V		Het
B4galnt1	A	G	10: 127,007,485 (GRCm39)	E462G	possibly damaging	Het
BC048671	T	A	6: 90,280,273 (GRCm39)	V63E	probably damaging	Het
Bod1l	A	G	5: 41,988,889 (GRCm39)	V406A	probably damaging	Het
Btn1a1	T	G	13: 23,645,668 (GRCm39)	I234L	probably benign	Het
Camk2b	A	C	11: 5,928,432 (GRCm39)	S436A	probably benign	Het
Cc2d1a	A	T	8: 84,870,374 (GRCm39)		probably null	Het
Ces1b	G	T	8: 93,789,757 (GRCm39)	N390K	probably benign	Het
Chpf	T	C	1: 75,451,927 (GRCm39)	Q671R	probably benign	Het
Cspg4b	T	A	13: 113,455,524 (GRCm39)	S523R		Het
Dbr1	C	A	9: 99,465,443 (GRCm39)	N340K	probably damaging	Het
Dram1	T	A	10: 88,176,507 (GRCm39)	T73S	probably benign	Het
Efemp1	A	T	11: 28,817,501 (GRCm39)	R39S	possibly damaging	Het
Eif2b5	T	G	16: 20,324,887 (GRCm39)	I515S	possibly damaging	Het
Eif2d	A	T	1: 131,094,111 (GRCm39)	N434I	probably benign	Het
Ercc6	C	T	14: 32,291,762 (GRCm39)	T1042I	probably benign	Het
Fbxw16	T	A	9: 109,278,091 (GRCm39)	N29I	probably damaging	Het
Fbxw18	T	C	9: 109,517,954 (GRCm39)	D344G	probably benign	Het
Fgf15	G	T	7: 144,453,542 (GRCm39)	V172L	probably benign	Het
Fmod	T	C	1: 133,967,978 (GRCm39)	V6A	probably benign	Het
Furin	C	A	7: 80,047,862 (GRCm39)	R86L	probably benign	Het
Gabrr1	T	C	4: 33,160,207 (GRCm39)	V297A	probably damaging	Het
Gbp10	G	A	5: 105,384,015 (GRCm39)		probably benign	Het
Helb	T	C	10: 119,925,476 (GRCm39)	D967G	probably benign	Het
Hmcn1	T	C	1: 150,439,382 (GRCm39)	T5601A	possibly damaging	Het
Hsbp1l1	G	T	18: 80,276,634 (GRCm39)	P70Q	not run	Het
Hsd17b1	T	C	11: 100,970,033 (GRCm39)	V155A	probably damaging	Het
Ireb2	T	A	9: 54,789,617 (GRCm39)	M97K	possibly damaging	Het
Kcnj6	C	A	16: 94,563,306 (GRCm39)	L397F	probably benign	Het
Lcorl	A	T	5: 46,014,801 (GRCm39)		probably null	Het
Lgi2	A	T	5: 52,695,753 (GRCm39)	I402N	probably damaging	Het
Lrat	T	A	3: 82,810,590 (GRCm39)	R144*	probably null	Het
Mfhas1	G	T	8: 36,057,353 (GRCm39)	L609F	probably damaging	Het
Mink1	T	A	11: 70,495,994 (GRCm39)	I398K	possibly damaging	Het
Muc5ac	A	T	7: 141,362,152 (GRCm39)	D1821V	unknown	Het
Myh2	T	C	11: 67,085,554 (GRCm39)		probably null	Het
Ncor1	T	C	11: 62,234,044 (GRCm39)	E386G	probably damaging	Het
Ndufaf3	C	T	9: 108,443,802 (GRCm39)	V76M	probably damaging	Het
Neurod4	T	C	10: 130,106,891 (GRCm39)	T128A	probably damaging	Het
Ogfod1	T	A	8: 94,765,615 (GRCm39)	D59E	probably benign	Het
Or2j6	C	T	7: 139,980,476 (GRCm39)	R161H	probably benign	Het
Or2w1b	T	C	13: 21,300,477 (GRCm39)	V205A	probably benign	Het
Or52n2b	C	T	7: 104,565,558 (GRCm39)	S315N	probably benign	Het
Pcdh15	T	A	10: 74,466,522 (GRCm39)	V1513D	probably benign	Het
Pclo	C	A	5: 14,589,902 (GRCm39)	T734K	unknown	Het
Phc2	A	G	4: 128,641,954 (GRCm39)	R759G	probably benign	Het
Plch1	A	T	3: 63,606,375 (GRCm39)	N1176K	probably benign	Het
Plec	T	A	15: 76,059,089 (GRCm39)	Y3616F	probably damaging	Het
Ptpn11	G	T	5: 121,282,707 (GRCm39)	T426N	probably benign	Het
Rictor	A	G	15: 6,816,462 (GRCm39)	T1245A	not run	Het
Rilp	T	C	11: 75,401,712 (GRCm39)	V164A	probably damaging	Het
Rnf39	A	T	17: 37,257,971 (GRCm39)	T168S	probably damaging	Het
Rptor	C	G	11: 119,763,181 (GRCm39)	Q922E	probably benign	Het
Sall1	A	T	8: 89,759,396 (GRCm39)	L236Q	probably damaging	Het
Skint7	A	G	4: 111,845,324 (GRCm39)	T379A	probably benign	Het
Spata22	C	A	11: 73,236,702 (GRCm39)	T336N	probably damaging	Het
Spata31	T	C	13: 65,068,547 (GRCm39)	S232P	probably benign	Het
Spink5	T	A	18: 44,110,722 (GRCm39)	C98S	possibly damaging	Het
Stard6	T	C	18: 70,633,506 (GRCm39)	V171A	possibly damaging	Het
Tex9	A	T	9: 72,388,072 (GRCm39)		probably null	Het
Traf7	T	C	17: 24,728,519 (GRCm39)	D621G	probably damaging	Het
Trak1	C	T	9: 121,277,973 (GRCm39)	T353M	possibly damaging	Het
Trim68	A	T	7: 102,327,569 (GRCm39)	Y461*	probably null	Het
Usp1	A	G	4: 98,814,688 (GRCm39)		probably benign	Het
Vmn2r42	T	A	7: 8,195,641 (GRCm39)	I502F	probably benign	Het
Wbp4	C	A	14: 79,714,261 (GRCm39)	G84C	probably damaging	Het
Zfp764l1	C	T	7: 126,992,496 (GRCm39)	C38Y	probably null	Het
Zfp787	A	G	7: 6,135,106 (GRCm39)	*382Q	probably null	Het
Zfp831	G	T	2: 174,487,002 (GRCm39)	C559F	possibly damaging	Het
Zgpat	G	C	2: 181,007,882 (GRCm39)	A140P	probably benign	Het
Zscan4e	T	A	7: 11,041,002 (GRCm39)	Y290F	probably benign	Het

Other mutations in Etl4

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00907:Etl4	APN	2	20,771,289 (GRCm39)	missense	possibly damaging	0.81
IGL00944:Etl4	APN	2	20,534,865 (GRCm39)	missense	possibly damaging	0.52
IGL01078:Etl4	APN	2	20,811,342 (GRCm39)	nonsense	probably null
IGL01099:Etl4	APN	2	20,811,922 (GRCm39)	missense	probably benign	0.06
IGL01337:Etl4	APN	2	20,790,198 (GRCm39)	missense	probably benign	0.01
IGL01348:Etl4	APN	2	20,811,784 (GRCm39)	missense	probably damaging	1.00
IGL01349:Etl4	APN	2	20,718,207 (GRCm39)	missense	probably damaging	1.00
IGL01407:Etl4	APN	2	20,748,667 (GRCm39)	missense	probably damaging	0.99
IGL01552:Etl4	APN	2	20,783,000 (GRCm39)	missense	probably damaging	0.99
IGL01662:Etl4	APN	2	20,811,460 (GRCm39)	missense	probably benign	0.04
IGL01687:Etl4	APN	2	20,534,898 (GRCm39)	missense	probably damaging	1.00
IGL01793:Etl4	APN	2	20,748,709 (GRCm39)	missense	possibly damaging	0.87
IGL01844:Etl4	APN	2	20,811,493 (GRCm39)	missense	probably benign	0.06
IGL02025:Etl4	APN	2	20,811,337 (GRCm39)	missense	probably damaging	1.00
IGL02088:Etl4	APN	2	20,811,359 (GRCm39)	missense	probably damaging	1.00
IGL02134:Etl4	APN	2	20,811,240 (GRCm39)	missense	possibly damaging	0.79
IGL02369:Etl4	APN	2	20,535,000 (GRCm39)	missense	probably damaging	1.00
IGL02480:Etl4	APN	2	20,793,335 (GRCm39)	missense	probably damaging	0.99
IGL02560:Etl4	APN	2	20,748,529 (GRCm39)	missense	probably damaging	1.00
IGL02851:Etl4	APN	2	20,812,840 (GRCm39)	missense	possibly damaging	0.46
IGL02893:Etl4	APN	2	20,765,021 (GRCm39)	splice site	probably benign
IGL02951:Etl4	APN	2	20,806,348 (GRCm39)	splice site	probably benign
IGL03119:Etl4	APN	2	20,718,198 (GRCm39)	missense	probably damaging	1.00
IGL03267:Etl4	APN	2	20,789,993 (GRCm39)	nonsense	probably null
IGL03379:Etl4	APN	2	20,666,827 (GRCm39)	missense	possibly damaging	0.87
R0038:Etl4	UTSW	2	20,748,385 (GRCm39)	missense	probably damaging	1.00
R0038:Etl4	UTSW	2	20,748,385 (GRCm39)	missense	probably damaging	1.00
R0095:Etl4	UTSW	2	20,748,679 (GRCm39)	missense	probably damaging	1.00
R0100:Etl4	UTSW	2	20,344,716 (GRCm39)	missense	probably benign
R0311:Etl4	UTSW	2	20,811,940 (GRCm39)	missense	probably damaging	1.00
R0346:Etl4	UTSW	2	20,764,463 (GRCm39)	critical splice donor site	probably null
R0348:Etl4	UTSW	2	20,782,940 (GRCm39)	missense	probably damaging	1.00
R0379:Etl4	UTSW	2	20,812,165 (GRCm39)	missense	probably damaging	0.98
R0571:Etl4	UTSW	2	20,748,580 (GRCm39)	missense	probably damaging	0.99
R0697:Etl4	UTSW	2	20,748,672 (GRCm39)	missense	probably damaging	1.00
R0707:Etl4	UTSW	2	20,810,382 (GRCm39)	splice site	probably benign
R0980:Etl4	UTSW	2	20,806,378 (GRCm39)	missense	probably damaging	1.00
R1120:Etl4	UTSW	2	20,811,514 (GRCm39)	missense	probably benign	0.00
R1254:Etl4	UTSW	2	20,812,734 (GRCm39)	missense	probably damaging	1.00
R1346:Etl4	UTSW	2	20,810,955 (GRCm39)	missense	possibly damaging	0.94
R1460:Etl4	UTSW	2	20,793,288 (GRCm39)	missense	probably damaging	1.00
R1503:Etl4	UTSW	2	20,748,685 (GRCm39)	missense	possibly damaging	0.94
R1547:Etl4	UTSW	2	20,790,039 (GRCm39)	missense	probably damaging	1.00
R1627:Etl4	UTSW	2	20,806,390 (GRCm39)	missense	possibly damaging	0.91
R1635:Etl4	UTSW	2	20,811,219 (GRCm39)	missense	probably damaging	1.00
R1716:Etl4	UTSW	2	20,748,492 (GRCm39)	missense	probably damaging	1.00
R1795:Etl4	UTSW	2	20,812,837 (GRCm39)	critical splice donor site	probably null
R1885:Etl4	UTSW	2	20,748,795 (GRCm39)	missense	probably damaging	1.00
R2039:Etl4	UTSW	2	20,790,039 (GRCm39)	missense	probably damaging	1.00
R2083:Etl4	UTSW	2	20,748,360 (GRCm39)	missense	probably damaging	1.00
R2109:Etl4	UTSW	2	20,790,153 (GRCm39)	missense	probably benign	0.27
R2153:Etl4	UTSW	2	20,803,545 (GRCm39)	missense	probably benign	0.00
R2403:Etl4	UTSW	2	20,812,117 (GRCm39)	nonsense	probably null
R2883:Etl4	UTSW	2	20,810,985 (GRCm39)	missense	possibly damaging	0.83
R2985:Etl4	UTSW	2	20,786,660 (GRCm39)	missense	probably damaging	1.00
R3402:Etl4	UTSW	2	20,786,693 (GRCm39)	missense	probably damaging	1.00
R3696:Etl4	UTSW	2	20,806,473 (GRCm39)	critical splice donor site	probably null
R3755:Etl4	UTSW	2	20,748,348 (GRCm39)	missense	probably benign	0.10
R3813:Etl4	UTSW	2	20,793,246 (GRCm39)	missense	probably damaging	1.00
R3829:Etl4	UTSW	2	20,790,232 (GRCm39)	missense	probably benign	0.07
R3887:Etl4	UTSW	2	20,534,772 (GRCm39)	nonsense	probably null
R3888:Etl4	UTSW	2	20,534,772 (GRCm39)	nonsense	probably null
R3889:Etl4	UTSW	2	20,534,772 (GRCm39)	nonsense	probably null
R3958:Etl4	UTSW	2	20,344,854 (GRCm39)	missense	probably benign
R3959:Etl4	UTSW	2	20,344,854 (GRCm39)	missense	probably benign
R3960:Etl4	UTSW	2	20,344,854 (GRCm39)	missense	probably benign
R4058:Etl4	UTSW	2	20,810,830 (GRCm39)	missense	possibly damaging	0.59
R4074:Etl4	UTSW	2	20,814,030 (GRCm39)	utr 3 prime	probably benign
R4077:Etl4	UTSW	2	20,812,772 (GRCm39)	missense	probably damaging	1.00
R4078:Etl4	UTSW	2	20,812,772 (GRCm39)	missense	probably damaging	1.00
R4127:Etl4	UTSW	2	20,748,886 (GRCm39)	missense	possibly damaging	0.93
R4200:Etl4	UTSW	2	20,786,694 (GRCm39)	missense	probably damaging	1.00
R4492:Etl4	UTSW	2	20,811,676 (GRCm39)	missense	possibly damaging	0.67
R4514:Etl4	UTSW	2	20,666,709 (GRCm39)	missense	probably damaging	1.00
R4820:Etl4	UTSW	2	20,811,496 (GRCm39)	missense	possibly damaging	0.85
R4825:Etl4	UTSW	2	20,811,738 (GRCm39)	missense	probably damaging	1.00
R4888:Etl4	UTSW	2	20,344,922 (GRCm39)	critical splice donor site	probably null
R4938:Etl4	UTSW	2	20,803,460 (GRCm39)	missense	probably benign	0.00
R4943:Etl4	UTSW	2	20,812,092 (GRCm39)	missense	probably benign	0.05
R5121:Etl4	UTSW	2	20,344,922 (GRCm39)	critical splice donor site	probably null
R5191:Etl4	UTSW	2	20,344,810 (GRCm39)	missense	probably damaging	0.99
R5198:Etl4	UTSW	2	20,718,198 (GRCm39)	missense	probably damaging	1.00
R5199:Etl4	UTSW	2	20,748,853 (GRCm39)	missense	probably damaging	1.00
R5470:Etl4	UTSW	2	20,534,791 (GRCm39)	missense	probably damaging	0.99
R5513:Etl4	UTSW	2	20,748,638 (GRCm39)	missense	probably damaging	1.00
R5620:Etl4	UTSW	2	20,535,037 (GRCm39)	missense	probably damaging	1.00
R5635:Etl4	UTSW	2	20,811,846 (GRCm39)	missense	probably damaging	1.00
R5641:Etl4	UTSW	2	20,811,273 (GRCm39)	frame shift	probably null
R5690:Etl4	UTSW	2	20,810,647 (GRCm39)	missense	probably benign	0.01
R5784:Etl4	UTSW	2	20,811,016 (GRCm39)	missense	possibly damaging	0.79
R5794:Etl4	UTSW	2	20,811,323 (GRCm39)	missense	probably damaging	1.00
R5908:Etl4	UTSW	2	20,748,718 (GRCm39)	missense	probably damaging	0.96
R5982:Etl4	UTSW	2	20,785,826 (GRCm39)	missense	probably damaging	1.00
R6151:Etl4	UTSW	2	20,718,171 (GRCm39)	missense	probably damaging	1.00
R6192:Etl4	UTSW	2	20,806,362 (GRCm39)	missense	probably damaging	0.98
R6238:Etl4	UTSW	2	20,806,379 (GRCm39)	missense	probably damaging	1.00
R6248:Etl4	UTSW	2	20,813,900 (GRCm39)	missense	possibly damaging	0.90
R6292:Etl4	UTSW	2	20,748,384 (GRCm39)	missense	probably damaging	1.00
R6610:Etl4	UTSW	2	20,718,180 (GRCm39)	missense	probably damaging	1.00
R6739:Etl4	UTSW	2	20,718,246 (GRCm39)	missense	probably damaging	1.00
R6846:Etl4	UTSW	2	20,748,919 (GRCm39)	missense	possibly damaging	0.94
R6863:Etl4	UTSW	2	20,811,120 (GRCm39)	missense	probably benign	0.01
R6873:Etl4	UTSW	2	20,802,803 (GRCm39)	splice site	probably null
R7003:Etl4	UTSW	2	20,810,695 (GRCm39)	missense	probably benign	0.03
R7155:Etl4	UTSW	2	20,811,742 (GRCm39)	missense	probably damaging	0.96
R7207:Etl4	UTSW	2	20,714,387 (GRCm39)	missense	probably damaging	0.99
R7230:Etl4	UTSW	2	20,802,799 (GRCm39)	missense	probably damaging	1.00
R7305:Etl4	UTSW	2	20,714,368 (GRCm39)	missense	probably damaging	1.00
R7389:Etl4	UTSW	2	20,789,904 (GRCm39)	nonsense	probably null
R7441:Etl4	UTSW	2	20,749,000 (GRCm39)	missense	possibly damaging	0.87
R7626:Etl4	UTSW	2	20,718,189 (GRCm39)	missense	probably damaging	1.00
R7776:Etl4	UTSW	2	20,811,957 (GRCm39)	missense	probably damaging	0.99
R7779:Etl4	UTSW	2	20,714,288 (GRCm39)	missense	probably damaging	1.00
R7798:Etl4	UTSW	2	20,786,757 (GRCm39)	critical splice donor site	probably null
R7851:Etl4	UTSW	2	20,748,951 (GRCm39)	missense	probably damaging	1.00
R7861:Etl4	UTSW	2	20,810,721 (GRCm39)	missense	probably benign
R7901:Etl4	UTSW	2	20,294,821 (GRCm39)	missense	possibly damaging	0.83
R8053:Etl4	UTSW	2	20,666,774 (GRCm39)	missense	probably damaging	1.00
R8124:Etl4	UTSW	2	20,811,451 (GRCm39)	missense	probably benign	0.06
R8133:Etl4	UTSW	2	20,811,082 (GRCm39)	missense	possibly damaging	0.86
R8203:Etl4	UTSW	2	20,789,916 (GRCm39)	missense	possibly damaging	0.61
R8238:Etl4	UTSW	2	20,811,342 (GRCm39)	nonsense	probably null
R8263:Etl4	UTSW	2	20,748,965 (GRCm39)	missense	probably benign	0.00
R8299:Etl4	UTSW	2	20,748,874 (GRCm39)	missense	possibly damaging	0.81
R8318:Etl4	UTSW	2	20,793,341 (GRCm39)	missense	probably damaging	1.00
R8334:Etl4	UTSW	2	20,785,857 (GRCm39)	missense	probably damaging	0.96
R8443:Etl4	UTSW	2	20,810,977 (GRCm39)	missense	probably benign	0.04
R8525:Etl4	UTSW	2	20,534,892 (GRCm39)	missense	probably damaging	1.00
R8679:Etl4	UTSW	2	20,714,288 (GRCm39)	missense	probably damaging	1.00
R8918:Etl4	UTSW	2	20,811,246 (GRCm39)	missense	probably benign	0.00
R8918:Etl4	UTSW	2	20,748,733 (GRCm39)	missense	probably benign
R9062:Etl4	UTSW	2	20,748,616 (GRCm39)	missense	probably damaging	0.99
R9095:Etl4	UTSW	2	20,782,964 (GRCm39)	missense	probably damaging	1.00
R9200:Etl4	UTSW	2	20,786,702 (GRCm39)	missense	probably damaging	1.00
R9416:Etl4	UTSW	2	20,748,784 (GRCm39)	missense	probably benign	0.17
R9437:Etl4	UTSW	2	20,813,872 (GRCm39)	missense	probably benign	0.20
R9451:Etl4	UTSW	2	20,813,926 (GRCm39)	missense	probably benign	0.03
R9489:Etl4	UTSW	2	20,771,345 (GRCm39)	missense	possibly damaging	0.75
R9531:Etl4	UTSW	2	20,294,818 (GRCm39)	start codon destroyed	probably null	0.01
R9605:Etl4	UTSW	2	20,771,345 (GRCm39)	missense	possibly damaging	0.75
R9623:Etl4	UTSW	2	20,811,052 (GRCm39)	missense
R9631:Etl4	UTSW	2	20,666,749 (GRCm39)	missense	probably benign	0.28
R9632:Etl4	UTSW	2	20,666,749 (GRCm39)	missense	probably benign	0.28
R9646:Etl4	UTSW	2	20,802,724 (GRCm39)	missense	probably benign	0.00
R9732:Etl4	UTSW	2	20,748,373 (GRCm39)	missense	probably damaging	0.98
R9755:Etl4	UTSW	2	20,790,048 (GRCm39)	missense	probably benign	0.17
R9771:Etl4	UTSW	2	20,811,537 (GRCm39)	missense	probably benign
RF003:Etl4	UTSW	2	20,524,729 (GRCm39)	nonsense	probably null
X0018:Etl4	UTSW	2	20,814,001 (GRCm39)	missense	probably damaging	0.98
X0022:Etl4	UTSW	2	20,714,375 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- AAGGCCCTTTATAAGCGTGGG -3'
(R):5'- CCAAGTGTCACCTCAGCTAC -3'

Sequencing Primer
(F):5'- CCCTTTATAAGCGTGGGCAATTTAG -3'
(R):5'- CAGCTACGTACCTGGAGTTCTG -3'

Posted On

2019-09-13