Incidental Mutation 'R7409:Bltp2'
| ID |
574940 |
| Institutional Source |
Beutler Lab
|
| Gene Symbol |
Bltp2
|
| Ensembl Gene |
ENSMUSG00000010277 |
| Gene Name |
bridge-like lipid transfer protein family member 2 |
| Synonyms |
2610507B11Rik |
| MMRRC Submission |
045490-MU
|
| Accession Numbers |
|
| Essential gene? |
Probably essential
(E-score: 0.954)
|
| Stock # |
R7409 (G1)
|
| Quality Score |
225.009 |
|
Status
|
Validated
|
| Chromosome |
11 |
| Chromosomal Location |
78152578-78181449 bp(+) (GRCm39) |
| Type of Mutation |
missense |
| DNA Base Change (assembly) |
G to T
at 78159583 bp (GRCm39)
|
| Zygosity |
Heterozygous |
| Amino Acid Change |
Arginine to Leucine
at position 544
(R544L)
|
| Ref Sequence |
ENSEMBL: ENSMUSP00000010421
(fasta)
|
| Gene Model |
predicted gene model for transcript(s):
[ENSMUST00000010421]
|
| AlphaFold |
Q5SYL3 |
| Predicted Effect |
probably damaging
Transcript: ENSMUST00000010421
AA Change: R544L
PolyPhen 2
Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)
|
| SMART Domains |
Protein: ENSMUSP00000010421
Gene: ENSMUSG00000010277
AA Change: R544L
| Domain | Start | End | E-Value | Type |
|---|
|
low complexity region
|
3 |
21 |
N/A |
INTRINSIC |
|
Pfam:Fmp27
|
26 |
475 |
1.6e-45 |
PFAM |
|
Pfam:Fmp27
|
446 |
674 |
3.2e-24 |
PFAM |
|
low complexity region
|
719 |
734 |
N/A |
INTRINSIC |
|
low complexity region
|
785 |
798 |
N/A |
INTRINSIC |
|
low complexity region
|
859 |
872 |
N/A |
INTRINSIC |
|
Fmp27_GFWDK
|
1028 |
1160 |
3.01e-61 |
SMART |
|
low complexity region
|
1415 |
1421 |
N/A |
INTRINSIC |
|
low complexity region
|
1690 |
1701 |
N/A |
INTRINSIC |
|
Pfam:Apt1
|
1703 |
2176 |
2.4e-112 |
PFAM |
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000145145
|
| SMART Domains |
Protein: ENSMUSP00000119105
Gene: ENSMUSG00000010277
| Domain | Start | End | E-Value | Type |
|---|
|
Pfam:Fmp27_GFWDK
|
1 |
52 |
1.2e-20 |
PFAM |
|
| Coding Region Coverage |
- 1x: 100.0%
- 3x: 99.9%
- 10x: 99.6%
- 20x: 98.6%
|
| Validation Efficiency |
98% (82/84) |
| Allele List at MGI |
|
| Other mutations in this stock |
Total: 84 list
| Gene | Ref | Var | Chr/Loc | Mutation | Predicted Effect | Zygosity |
|---|
| Adgrb2 |
C |
T |
4: 129,912,862 (GRCm39) |
A1329V |
probably benign |
Het |
| Aida |
A |
G |
1: 183,099,809 (GRCm39) |
T215A |
probably benign |
Het |
| Alpk2 |
A |
G |
18: 65,440,023 (GRCm39) |
S457P |
probably benign |
Het |
| Ap4b1 |
G |
A |
3: 103,719,474 (GRCm39) |
V63I |
probably damaging |
Het |
| Apaf1 |
A |
G |
10: 90,903,108 (GRCm39) |
V182A |
probably damaging |
Het |
| B4galnt4 |
G |
A |
7: 140,646,916 (GRCm39) |
|
probably null |
Het |
| Cacna1a |
T |
G |
8: 85,260,031 (GRCm39) |
D331E |
probably damaging |
Het |
| Carmil2 |
A |
G |
8: 106,419,423 (GRCm39) |
|
probably null |
Het |
| Cdkn1b |
A |
T |
6: 134,898,280 (GRCm39) |
Q133L |
probably benign |
Het |
| Cep192 |
G |
C |
18: 67,967,874 (GRCm39) |
S786T |
possibly damaging |
Het |
| Cfap418 |
T |
A |
4: 10,881,834 (GRCm39) |
C94S |
probably benign |
Het |
| Cfap97 |
G |
A |
8: 46,645,733 (GRCm39) |
R537H |
probably benign |
Het |
| Clpx |
C |
G |
9: 65,231,529 (GRCm39) |
A552G |
possibly damaging |
Het |
| Cryl1 |
G |
A |
14: 57,523,842 (GRCm39) |
T240I |
probably damaging |
Het |
| Ddx60 |
C |
A |
8: 62,411,612 (GRCm39) |
T488K |
probably benign |
Het |
| Dennd4b |
A |
G |
3: 90,181,259 (GRCm39) |
H805R |
probably benign |
Het |
| Dnmbp |
T |
C |
19: 43,878,996 (GRCm39) |
D25G |
unknown |
Het |
| Dysf |
A |
C |
6: 84,126,664 (GRCm39) |
D1293A |
probably benign |
Het |
| Efl1 |
T |
C |
7: 82,347,121 (GRCm39) |
L549P |
probably damaging |
Het |
| Eif5 |
T |
C |
12: 111,506,697 (GRCm39) |
|
probably benign |
Het |
| Eva1c |
A |
G |
16: 90,666,544 (GRCm39) |
K156E |
probably damaging |
Het |
| Fbxw10 |
T |
G |
11: 62,767,606 (GRCm39) |
V814G |
possibly damaging |
Het |
| Gfap |
C |
T |
11: 102,785,358 (GRCm39) |
R206Q |
probably benign |
Het |
| Gjb6 |
A |
T |
14: 57,361,610 (GRCm39) |
L217* |
probably null |
Het |
| Gpatch11 |
T |
A |
17: 79,146,595 (GRCm39) |
L80Q |
probably damaging |
Het |
| Gramd1b |
T |
C |
9: 40,238,727 (GRCm39) |
Q225R |
probably damaging |
Het |
| Gsdmc2 |
A |
G |
15: 63,705,195 (GRCm39) |
S173P |
possibly damaging |
Het |
| Hars1 |
C |
G |
18: 36,903,166 (GRCm39) |
R388P |
probably damaging |
Het |
| Ighm |
C |
T |
12: 113,385,852 (GRCm39) |
R129H |
|
Het |
| Igsf9 |
A |
G |
1: 172,322,841 (GRCm39) |
I602V |
probably benign |
Het |
| Inpp4b |
T |
G |
8: 82,679,314 (GRCm39) |
|
probably null |
Het |
| Itch |
C |
A |
2: 155,041,302 (GRCm39) |
T450K |
probably damaging |
Het |
| Kcnq1 |
T |
C |
7: 142,663,152 (GRCm39) |
F20L |
unknown |
Het |
| Kmt2d |
G |
T |
15: 98,753,235 (GRCm39) |
A153E |
probably damaging |
Het |
| Macf1 |
T |
A |
4: 123,398,263 (GRCm39) |
N750I |
probably damaging |
Het |
| Marveld2 |
T |
A |
13: 100,747,984 (GRCm39) |
H365L |
probably damaging |
Het |
| Med13l |
T |
A |
5: 118,892,386 (GRCm39) |
D1936E |
probably benign |
Het |
| Mettl8 |
A |
T |
2: 70,803,687 (GRCm39) |
V200E |
probably damaging |
Het |
| Mrgbp |
T |
G |
2: 180,227,135 (GRCm39) |
S157A |
possibly damaging |
Het |
| Mrps35 |
A |
G |
6: 146,957,481 (GRCm39) |
T169A |
possibly damaging |
Het |
| Mycbp2 |
A |
T |
14: 103,526,180 (GRCm39) |
Y551N |
probably damaging |
Het |
| Myo18b |
T |
C |
5: 113,021,971 (GRCm39) |
R474G |
probably benign |
Het |
| Nfx1 |
T |
A |
4: 41,021,830 (GRCm39) |
S979R |
possibly damaging |
Het |
| Nlrp1a |
T |
C |
11: 71,013,634 (GRCm39) |
T539A |
probably benign |
Het |
| Oca2 |
T |
A |
7: 56,064,145 (GRCm39) |
D713E |
probably benign |
Het |
| Omt2b |
A |
C |
9: 78,235,894 (GRCm39) |
Y73S |
probably benign |
Het |
| Or10aa1 |
T |
A |
1: 173,870,099 (GRCm39) |
H194Q |
probably benign |
Het |
| Or13a27 |
C |
T |
7: 139,925,318 (GRCm39) |
V195I |
probably benign |
Het |
| Or6c38 |
A |
G |
10: 128,929,081 (GRCm39) |
I254T |
probably damaging |
Het |
| Or6c69 |
A |
T |
10: 129,748,120 (GRCm39) |
V9D |
possibly damaging |
Het |
| Pde11a |
G |
T |
2: 75,836,328 (GRCm39) |
Q20K |
|
Het |
| Peg10 |
CCACATCAGGATCCACATCAGGATGCACATCAGCATCAGGATCCCCATCAGGATGCACATCAGGATCCACATCAGGATGCACATCAG |
CCACATCAGGATCCACATCAGGATGCACATCAG |
6: 4,756,398 (GRCm39) |
|
probably benign |
Het |
| Phrf1 |
C |
A |
7: 140,839,205 (GRCm39) |
T800K |
unknown |
Het |
| Polr1f |
T |
G |
12: 33,486,988 (GRCm39) |
C150W |
possibly damaging |
Het |
| Pramel4 |
T |
C |
4: 143,795,061 (GRCm39) |
S486P |
probably benign |
Het |
| Proc |
T |
C |
18: 32,260,513 (GRCm39) |
D204G |
probably benign |
Het |
| Rasgrp3 |
T |
A |
17: 75,823,411 (GRCm39) |
I494N |
possibly damaging |
Het |
| Samm50 |
A |
G |
15: 84,081,231 (GRCm39) |
D53G |
probably benign |
Het |
| Satb1 |
T |
C |
17: 52,116,217 (GRCm39) |
D22G |
possibly damaging |
Het |
| Scarf2 |
T |
C |
16: 17,624,918 (GRCm39) |
S658P |
probably damaging |
Het |
| Sfta2 |
T |
A |
17: 35,925,410 (GRCm39) |
I29K |
unknown |
Het |
| Slc15a4 |
A |
T |
5: 127,681,742 (GRCm39) |
S292T |
probably benign |
Het |
| Slc37a1 |
C |
T |
17: 31,559,237 (GRCm39) |
T439I |
probably damaging |
Het |
| Slc4a9 |
C |
A |
18: 36,663,858 (GRCm39) |
P294Q |
probably damaging |
Het |
| Slc52a3 |
T |
C |
2: 151,846,086 (GRCm39) |
S16P |
probably damaging |
Het |
| Slc6a15 |
A |
G |
10: 103,244,163 (GRCm39) |
I468V |
probably benign |
Het |
| Spag17 |
T |
C |
3: 99,934,547 (GRCm39) |
S610P |
possibly damaging |
Het |
| Spag17 |
A |
T |
3: 99,941,475 (GRCm39) |
D738V |
probably benign |
Het |
| Ssbp4 |
T |
C |
8: 71,050,617 (GRCm39) |
R269G |
unknown |
Het |
| Tbl1xr1 |
A |
G |
3: 22,257,354 (GRCm39) |
T406A |
possibly damaging |
Het |
| Tep1 |
A |
T |
14: 51,104,312 (GRCm39) |
V194D |
possibly damaging |
Het |
| Thbs4 |
A |
T |
13: 92,909,767 (GRCm39) |
C343* |
probably null |
Het |
| Tmed10 |
A |
T |
12: 85,391,065 (GRCm39) |
S158T |
possibly damaging |
Het |
| Trbv3 |
T |
A |
6: 41,025,524 (GRCm39) |
V38E |
probably damaging |
Het |
| Ttc6 |
T |
C |
12: 57,743,772 (GRCm39) |
M1258T |
probably damaging |
Het |
| Ttn |
T |
C |
2: 76,589,320 (GRCm39) |
D21281G |
probably damaging |
Het |
| Usp54 |
T |
C |
14: 20,602,313 (GRCm39) |
R1346G |
probably damaging |
Het |
| Vmn2r8 |
C |
A |
5: 108,956,449 (GRCm39) |
E58* |
probably null |
Het |
| Vps13d |
C |
T |
4: 144,867,824 (GRCm39) |
E2009K |
|
Het |
| Vps33b |
T |
C |
7: 79,935,017 (GRCm39) |
I320T |
probably damaging |
Het |
| Vwa8 |
A |
C |
14: 79,219,674 (GRCm39) |
|
probably null |
Het |
| Ythdf1 |
T |
C |
2: 180,553,786 (GRCm39) |
Y143C |
probably damaging |
Het |
| Zfp213 |
C |
T |
17: 23,778,603 (GRCm39) |
|
probably null |
Het |
| Zfp219 |
A |
T |
14: 52,244,570 (GRCm39) |
Y536* |
probably null |
Het |
|
| Other mutations in Bltp2 |
| Allele | Source | Chr | Coord | Type | Predicted Effect | PPH Score |
|---|
|
IGL00334:Bltp2
|
APN |
11 |
78,160,400 (GRCm39) |
missense |
possibly damaging |
0.55 |
|
IGL00497:Bltp2
|
APN |
11 |
78,163,759 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL00797:Bltp2
|
APN |
11 |
78,163,976 (GRCm39) |
missense |
probably benign |
0.07 |
|
IGL01695:Bltp2
|
APN |
11 |
78,156,019 (GRCm39) |
missense |
probably benign |
0.03 |
|
IGL02055:Bltp2
|
APN |
11 |
78,177,457 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL02066:Bltp2
|
APN |
11 |
78,164,058 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL02231:Bltp2
|
APN |
11 |
78,170,722 (GRCm39) |
missense |
probably benign |
|
|
IGL02282:Bltp2
|
APN |
11 |
78,175,054 (GRCm39) |
missense |
probably benign |
0.22 |
|
IGL02293:Bltp2
|
APN |
11 |
78,162,736 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL02336:Bltp2
|
APN |
11 |
78,179,858 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL02528:Bltp2
|
APN |
11 |
78,162,802 (GRCm39) |
missense |
possibly damaging |
0.93 |
|
IGL03231:Bltp2
|
APN |
11 |
78,159,528 (GRCm39) |
missense |
probably benign |
0.02 |
|
R0003:Bltp2
|
UTSW |
11 |
78,177,404 (GRCm39) |
missense |
possibly damaging |
0.66 |
|
R0197:Bltp2
|
UTSW |
11 |
78,160,530 (GRCm39) |
unclassified |
probably benign |
|
|
R0244:Bltp2
|
UTSW |
11 |
78,177,317 (GRCm39) |
splice site |
probably null |
|
|
R0281:Bltp2
|
UTSW |
11 |
78,162,750 (GRCm39) |
missense |
possibly damaging |
0.88 |
|
R0396:Bltp2
|
UTSW |
11 |
78,159,203 (GRCm39) |
missense |
possibly damaging |
0.93 |
|
R0624:Bltp2
|
UTSW |
11 |
78,159,283 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R0666:Bltp2
|
UTSW |
11 |
78,168,038 (GRCm39) |
nonsense |
probably null |
|
|
R0666:Bltp2
|
UTSW |
11 |
78,178,813 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R1313:Bltp2
|
UTSW |
11 |
78,156,498 (GRCm39) |
missense |
probably benign |
0.02 |
|
R1313:Bltp2
|
UTSW |
11 |
78,156,498 (GRCm39) |
missense |
probably benign |
0.02 |
|
R1443:Bltp2
|
UTSW |
11 |
78,153,624 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R1485:Bltp2
|
UTSW |
11 |
78,176,406 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R1500:Bltp2
|
UTSW |
11 |
78,174,958 (GRCm39) |
missense |
possibly damaging |
0.46 |
|
R1537:Bltp2
|
UTSW |
11 |
78,180,169 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R1543:Bltp2
|
UTSW |
11 |
78,166,000 (GRCm39) |
missense |
probably benign |
0.44 |
|
R1702:Bltp2
|
UTSW |
11 |
78,179,854 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R1804:Bltp2
|
UTSW |
11 |
78,164,295 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R1835:Bltp2
|
UTSW |
11 |
78,178,576 (GRCm39) |
missense |
probably damaging |
0.97 |
|
R1852:Bltp2
|
UTSW |
11 |
78,159,299 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R1861:Bltp2
|
UTSW |
11 |
78,178,755 (GRCm39) |
unclassified |
probably benign |
|
|
R1986:Bltp2
|
UTSW |
11 |
78,165,438 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R1987:Bltp2
|
UTSW |
11 |
78,158,993 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R2061:Bltp2
|
UTSW |
11 |
78,159,575 (GRCm39) |
nonsense |
probably null |
|
|
R2113:Bltp2
|
UTSW |
11 |
78,159,598 (GRCm39) |
missense |
probably benign |
0.02 |
|
R3692:Bltp2
|
UTSW |
11 |
78,160,335 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R3788:Bltp2
|
UTSW |
11 |
78,179,123 (GRCm39) |
critical splice donor site |
probably null |
|
|
R3835:Bltp2
|
UTSW |
11 |
78,169,911 (GRCm39) |
missense |
probably benign |
0.17 |
|
R3882:Bltp2
|
UTSW |
11 |
78,153,526 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R3943:Bltp2
|
UTSW |
11 |
78,160,350 (GRCm39) |
nonsense |
probably null |
|
|
R3944:Bltp2
|
UTSW |
11 |
78,160,350 (GRCm39) |
nonsense |
probably null |
|
|
R3945:Bltp2
|
UTSW |
11 |
78,180,790 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R4196:Bltp2
|
UTSW |
11 |
78,154,382 (GRCm39) |
intron |
probably benign |
|
|
R4510:Bltp2
|
UTSW |
11 |
78,168,154 (GRCm39) |
missense |
possibly damaging |
0.59 |
|
R4511:Bltp2
|
UTSW |
11 |
78,168,154 (GRCm39) |
missense |
possibly damaging |
0.59 |
|
R4756:Bltp2
|
UTSW |
11 |
78,154,854 (GRCm39) |
missense |
probably damaging |
0.98 |
|
R5337:Bltp2
|
UTSW |
11 |
78,156,034 (GRCm39) |
missense |
possibly damaging |
0.46 |
|
R5419:Bltp2
|
UTSW |
11 |
78,162,916 (GRCm39) |
nonsense |
probably null |
|
|
R5572:Bltp2
|
UTSW |
11 |
78,155,393 (GRCm39) |
missense |
probably damaging |
0.98 |
|
R5719:Bltp2
|
UTSW |
11 |
78,164,071 (GRCm39) |
missense |
probably damaging |
0.97 |
|
R5754:Bltp2
|
UTSW |
11 |
78,160,367 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R5890:Bltp2
|
UTSW |
11 |
78,164,096 (GRCm39) |
nonsense |
probably null |
|
|
R5919:Bltp2
|
UTSW |
11 |
78,180,176 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R5925:Bltp2
|
UTSW |
11 |
78,175,064 (GRCm39) |
missense |
probably benign |
0.06 |
|
R5976:Bltp2
|
UTSW |
11 |
78,174,955 (GRCm39) |
missense |
probably benign |
0.00 |
|
R5999:Bltp2
|
UTSW |
11 |
78,176,294 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R6056:Bltp2
|
UTSW |
11 |
78,162,210 (GRCm39) |
missense |
possibly damaging |
0.77 |
|
R6180:Bltp2
|
UTSW |
11 |
78,164,084 (GRCm39) |
missense |
possibly damaging |
0.51 |
|
R6484:Bltp2
|
UTSW |
11 |
78,169,921 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R6721:Bltp2
|
UTSW |
11 |
78,170,625 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R6800:Bltp2
|
UTSW |
11 |
78,179,105 (GRCm39) |
missense |
probably benign |
0.13 |
|
R6911:Bltp2
|
UTSW |
11 |
78,159,179 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R6923:Bltp2
|
UTSW |
11 |
78,165,452 (GRCm39) |
missense |
possibly damaging |
0.67 |
|
R7283:Bltp2
|
UTSW |
11 |
78,165,654 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R7287:Bltp2
|
UTSW |
11 |
78,163,709 (GRCm39) |
missense |
possibly damaging |
0.61 |
|
R7339:Bltp2
|
UTSW |
11 |
78,163,210 (GRCm39) |
critical splice donor site |
probably null |
|
|
R7473:Bltp2
|
UTSW |
11 |
78,157,941 (GRCm39) |
missense |
possibly damaging |
0.86 |
|
R7704:Bltp2
|
UTSW |
11 |
78,159,570 (GRCm39) |
missense |
probably benign |
0.29 |
|
R7793:Bltp2
|
UTSW |
11 |
78,164,031 (GRCm39) |
missense |
possibly damaging |
0.56 |
|
R8051:Bltp2
|
UTSW |
11 |
78,164,238 (GRCm39) |
intron |
probably benign |
|
|
R8186:Bltp2
|
UTSW |
11 |
78,177,457 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R8256:Bltp2
|
UTSW |
11 |
78,167,979 (GRCm39) |
missense |
probably benign |
0.00 |
|
R8518:Bltp2
|
UTSW |
11 |
78,156,064 (GRCm39) |
missense |
possibly damaging |
0.95 |
|
R8677:Bltp2
|
UTSW |
11 |
78,174,982 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R8736:Bltp2
|
UTSW |
11 |
78,178,875 (GRCm39) |
missense |
probably benign |
0.26 |
|
R8829:Bltp2
|
UTSW |
11 |
78,158,064 (GRCm39) |
missense |
probably benign |
0.02 |
|
R8832:Bltp2
|
UTSW |
11 |
78,158,064 (GRCm39) |
missense |
probably benign |
0.02 |
|
R9006:Bltp2
|
UTSW |
11 |
78,164,345 (GRCm39) |
missense |
possibly damaging |
0.90 |
|
R9014:Bltp2
|
UTSW |
11 |
78,160,488 (GRCm39) |
missense |
possibly damaging |
0.78 |
|
R9184:Bltp2
|
UTSW |
11 |
78,162,214 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R9473:Bltp2
|
UTSW |
11 |
78,174,983 (GRCm39) |
missense |
probably damaging |
1.00 |
|
X0028:Bltp2
|
UTSW |
11 |
78,177,461 (GRCm39) |
missense |
probably damaging |
0.99 |
|
| Predicted Primers |
PCR Primer
(F):5'- CTGCATCAGGGTCTCCTAAC -3'
(R):5'- TCCCTCTAACAAGGAGGCTACC -3'
Sequencing Primer
(F):5'- AGGGTCTCCTAACTGCATCG -3'
(R):5'- GGAGGCTACCCAGATAATAAGCTTC -3'
|
| Posted On |
2019-10-07 |
Incidental Mutation