Mutagenetix > Incidental Mutation

Incidental Mutation 'R7425:Virma'

575975

Institutional Source

Beutler Lab

Gene Symbol

Virma

Ensembl Gene

ENSMUSG00000040720

Gene Name

vir like m6A methyltransferase associated

Synonyms

1110037F02Rik

MMRRC Submission

045503-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R7425 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

11485958-11550684 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 11546211 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Asparagine at position 1683 (I1683N)

Ref Sequence

ENSEMBL: ENSMUSP00000058078 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000059914] [ENSMUST00000108307]

AlphaFold

A2AIV2

Predicted Effect

possibly damaging
Transcript: ENSMUST00000059914
AA Change: I1683N

PolyPhen 2 Score 0.951 (Sensitivity: 0.79; Specificity: 0.95)

SMART Domains

Protein: ENSMUSP00000058078
Gene: ENSMUSG00000040720
AA Change: I1683N

Domain	Start	End	E-Value	Type
low complexity region	139	153	N/A	INTRINSIC
low complexity region	172	198	N/A	INTRINSIC
low complexity region	236	267	N/A	INTRINSIC
low complexity region	276	297	N/A	INTRINSIC
low complexity region	615	625	N/A	INTRINSIC
low complexity region	1008	1020	N/A	INTRINSIC
low complexity region	1112	1124	N/A	INTRINSIC
low complexity region	1224	1232	N/A	INTRINSIC
low complexity region	1443	1458	N/A	INTRINSIC
low complexity region	1460	1474	N/A	INTRINSIC
low complexity region	1618	1634	N/A	INTRINSIC
low complexity region	1684	1697	N/A	INTRINSIC
low complexity region	1750	1757	N/A	INTRINSIC
low complexity region	1796	1808	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000108307
AA Change: I1733N

PolyPhen 2 Score 0.983 (Sensitivity: 0.75; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000103943
Gene: ENSMUSG00000040720
AA Change: I1733N

Domain	Start	End	E-Value	Type
Pfam:VIR_N	5	266	2e-110	PFAM
low complexity region	276	297	N/A	INTRINSIC
low complexity region	615	625	N/A	INTRINSIC
low complexity region	1058	1070	N/A	INTRINSIC
low complexity region	1162	1174	N/A	INTRINSIC
low complexity region	1274	1282	N/A	INTRINSIC
low complexity region	1493	1508	N/A	INTRINSIC
low complexity region	1510	1524	N/A	INTRINSIC
low complexity region	1668	1684	N/A	INTRINSIC
low complexity region	1734	1747	N/A	INTRINSIC
low complexity region	1800	1807	N/A	INTRINSIC
low complexity region	1846	1858	N/A	INTRINSIC

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.8%
20x: 99.3%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 68 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1110002E22Rik	A	G	3: 137,771,456 (GRCm39)	E215G	probably benign	Het
Adam8	T	A	7: 139,572,394 (GRCm39)		probably benign	Het
Ankrd35	A	G	3: 96,592,104 (GRCm39)	S797G	not run	Het
Anxa3	T	A	5: 96,982,680 (GRCm39)	H259Q	probably benign	Het
Ap3d1	T	A	10: 80,557,426 (GRCm39)	Q302L	probably damaging	Het
Atg2a	T	C	19: 6,305,682 (GRCm39)	V1294A	probably benign	Het
Atp13a5	G	A	16: 29,116,278 (GRCm39)	Q613*	probably null	Het
Bltp1	T	A	3: 37,002,490 (GRCm39)	H1478Q	probably benign	Het
Bltp1	A	T	3: 37,037,543 (GRCm39)	H2448L	probably benign	Het
Bmpr2	T	G	1: 59,906,510 (GRCm39)	N534K	probably benign	Het
C1qbp	T	C	11: 70,869,072 (GRCm39)		probably null	Het
C1qbp	G	T	11: 70,869,073 (GRCm39)		probably null	Het
C1ql3	T	G	2: 13,015,229 (GRCm39)	K144Q	possibly damaging	Het
C3	T	C	17: 57,511,039 (GRCm39)	M1656V	possibly damaging	Het
Cand1	A	T	10: 119,052,148 (GRCm39)	Y252N	probably benign	Het
Capn13	T	C	17: 73,625,053 (GRCm39)	I632M	probably benign	Het
Catsperb	A	G	12: 101,557,757 (GRCm39)	E776G	probably damaging	Het
Ccnl1	A	T	3: 65,856,179 (GRCm39)	V242D	probably damaging	Het
Cd68	T	C	11: 69,555,938 (GRCm39)	D200G	probably benign	Het
Cela3a	T	C	4: 137,132,899 (GRCm39)	N118D	probably benign	Het
Celsr2	A	G	3: 108,309,773 (GRCm39)	C1609R	probably damaging	Het
Cep85l	G	C	10: 53,177,666 (GRCm39)	Q458E	probably damaging	Het
Cnga1	T	G	5: 72,766,868 (GRCm39)	E190D	probably benign	Het
Cntnap3	G	A	13: 64,906,066 (GRCm39)	R847C	probably damaging	Het
Cntrob	G	A	11: 69,205,560 (GRCm39)	Q425*	probably null	Het
Commd9	G	A	2: 101,730,245 (GRCm39)	W128*	probably null	Het
Csnk1a1	C	T	18: 61,718,330 (GRCm39)	S352L	unknown	Het
Dact2	A	G	17: 14,416,593 (GRCm39)	S536P	probably damaging	Het
Ddx25	A	G	9: 35,465,882 (GRCm39)	I113T	probably benign	Het
Dscam	T	A	16: 96,430,598 (GRCm39)	D1630V	probably damaging	Het
Fndc7	A	C	3: 108,783,975 (GRCm39)	F211L	probably benign	Het
Fryl	T	C	5: 73,262,091 (GRCm39)	T559A	probably damaging	Het
Hpx	C	A	7: 105,241,068 (GRCm39)	D402Y	probably damaging	Het
Ints13	A	G	6: 146,476,198 (GRCm39)		probably null	Het
Ipcef1	T	C	10: 6,906,066 (GRCm39)	K76E	probably damaging	Het
Kcnb2	A	C	1: 15,780,031 (GRCm39)	Q301P	probably damaging	Het
Lamp3	C	T	16: 19,518,362 (GRCm39)		probably null	Het
Lmod2	A	T	6: 24,603,475 (GRCm39)	H150L	probably benign	Het
Lrch3	T	C	16: 32,826,077 (GRCm39)	F718S	probably damaging	Het
Mms22l	T	C	4: 24,596,287 (GRCm39)	V1082A	probably benign	Het
Nbas	G	T	12: 13,519,881 (GRCm39)	V1598L	probably damaging	Het
Nrxn3	C	T	12: 89,479,870 (GRCm39)	R671*	probably null	Het
Or4c110	A	T	2: 88,832,544 (GRCm39)	F29L		Het
Or4c58	A	G	2: 89,674,789 (GRCm39)	L176P	probably damaging	Het
Or52e8	A	T	7: 104,624,268 (GRCm39)	L312*	probably null	Het
Or5a3	A	C	19: 12,400,204 (GRCm39)	H177P	probably damaging	Het
Or6d14	A	T	6: 116,533,398 (GRCm39)	E4V	possibly damaging	Het
Pcdhga6	T	A	18: 37,841,619 (GRCm39)	N446K	probably damaging	Het
Phlpp1	A	T	1: 106,320,303 (GRCm39)	I1433F	probably benign	Het
Pla2g6	G	A	15: 79,192,933 (GRCm39)	R245C	probably damaging	Het
Rgl3	G	A	9: 21,888,123 (GRCm39)	Q464*	probably null	Het
Ryr2	T	C	13: 11,720,530 (GRCm39)	D2706G	probably benign	Het
Sbf2	G	A	7: 109,974,984 (GRCm39)	Q718*	probably null	Het
Sdad1	T	C	5: 92,447,980 (GRCm39)	T252A	probably benign	Het
Sgk1	T	C	10: 21,870,009 (GRCm39)	L16P	probably damaging	Het
Slc38a8	A	G	8: 120,212,327 (GRCm39)	S339P	possibly damaging	Het
Slc44a4	T	C	17: 35,140,667 (GRCm39)	S287P	possibly damaging	Het
Syne1	T	C	10: 5,375,760 (GRCm39)	I111V	probably damaging	Het
Synm	A	C	7: 67,383,194 (GRCm39)	S1489R	probably damaging	Het
Tfb2m	A	G	1: 179,365,269 (GRCm39)	F232L	probably benign	Het
Traf3	A	T	12: 111,227,095 (GRCm39)	K328*	probably null	Het
Trim38	C	A	13: 23,972,365 (GRCm39)	Q229K	probably benign	Het
Vcan	A	G	13: 89,837,951 (GRCm39)	I2531T	probably damaging	Het
Vmn1r18	A	T	6: 57,367,551 (GRCm39)	M1K	probably null	Het
Vmn2r87	T	C	10: 130,314,761 (GRCm39)	N275S	probably damaging	Het
Vps13a	T	A	19: 16,701,066 (GRCm39)	H701L	probably benign	Het
Vrk3	T	A	7: 44,420,348 (GRCm39)		probably null	Het
Zfp993	T	A	4: 146,742,098 (GRCm39)	S141T	possibly damaging	Het

Other mutations in Virma

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00428:Virma	APN	4	11,519,424 (GRCm39)	splice site	probably benign
IGL00477:Virma	APN	4	11,519,006 (GRCm39)	missense	probably damaging	0.99
IGL01293:Virma	APN	4	11,521,114 (GRCm39)	missense	probably damaging	1.00
IGL01410:Virma	APN	4	11,518,929 (GRCm39)	nonsense	probably null
IGL01531:Virma	APN	4	11,528,753 (GRCm39)	missense	probably damaging	1.00
IGL01672:Virma	APN	4	11,527,792 (GRCm39)	missense	probably damaging	1.00
IGL01724:Virma	APN	4	11,528,672 (GRCm39)	missense	probably damaging	1.00
IGL01747:Virma	APN	4	11,526,877 (GRCm39)	missense	probably damaging	1.00
IGL01776:Virma	APN	4	11,527,792 (GRCm39)	missense	probably damaging	1.00
IGL02064:Virma	APN	4	11,513,163 (GRCm39)	missense	possibly damaging	0.87
IGL02243:Virma	APN	4	11,546,031 (GRCm39)	missense	probably damaging	1.00
IGL02244:Virma	APN	4	11,546,031 (GRCm39)	missense	probably damaging	1.00
IGL02445:Virma	APN	4	11,527,029 (GRCm39)	missense	probably damaging	0.97
IGL02546:Virma	APN	4	11,494,804 (GRCm39)	missense	probably damaging	0.99
IGL02807:Virma	APN	4	11,507,079 (GRCm39)	splice site	probably benign
IGL02967:Virma	APN	4	11,514,096 (GRCm39)	missense	probably benign	0.01
IGL03211:Virma	APN	4	11,548,770 (GRCm39)	nonsense	probably null
IGL03242:Virma	APN	4	11,527,669 (GRCm39)	missense	possibly damaging	0.70
IGL03256:Virma	APN	4	11,542,207 (GRCm39)	splice site	probably benign
IGL03327:Virma	APN	4	11,518,984 (GRCm39)	missense	probably benign	0.00
IGL03346:Virma	APN	4	11,518,984 (GRCm39)	missense	probably benign	0.00
PIT4802001:Virma	UTSW	4	11,546,008 (GRCm39)	missense	probably damaging	0.99
R0142:Virma	UTSW	4	11,548,783 (GRCm39)	missense	probably benign	0.04
R0355:Virma	UTSW	4	11,528,626 (GRCm39)	nonsense	probably null
R0522:Virma	UTSW	4	11,519,416 (GRCm39)	critical splice donor site	probably null
R0600:Virma	UTSW	4	11,498,769 (GRCm39)	missense	probably damaging	0.99
R1435:Virma	UTSW	4	11,528,621 (GRCm39)	missense	probably damaging	1.00
R1489:Virma	UTSW	4	11,521,164 (GRCm39)	missense	probably damaging	1.00
R1568:Virma	UTSW	4	11,528,776 (GRCm39)	missense	probably damaging	0.99
R1616:Virma	UTSW	4	11,544,954 (GRCm39)	missense	probably damaging	1.00
R1655:Virma	UTSW	4	11,494,786 (GRCm39)	missense	probably damaging	1.00
R1695:Virma	UTSW	4	11,494,814 (GRCm39)	missense	probably damaging	0.98
R1835:Virma	UTSW	4	11,540,511 (GRCm39)	missense	probably benign	0.02
R1951:Virma	UTSW	4	11,513,907 (GRCm39)	missense	probably benign	0.00
R1991:Virma	UTSW	4	11,519,242 (GRCm39)	missense	probably benign	0.06
R2145:Virma	UTSW	4	11,548,726 (GRCm39)	splice site	probably benign
R2172:Virma	UTSW	4	11,527,843 (GRCm39)	missense	possibly damaging	0.82
R2217:Virma	UTSW	4	11,544,924 (GRCm39)	missense	probably damaging	1.00
R2218:Virma	UTSW	4	11,544,924 (GRCm39)	missense	probably damaging	1.00
R2248:Virma	UTSW	4	11,518,927 (GRCm39)	missense	probably damaging	1.00
R2342:Virma	UTSW	4	11,501,316 (GRCm39)	missense	probably damaging	1.00
R3424:Virma	UTSW	4	11,513,177 (GRCm39)	nonsense	probably null
R4397:Virma	UTSW	4	11,513,901 (GRCm39)	missense	possibly damaging	0.81
R4449:Virma	UTSW	4	11,498,828 (GRCm39)	critical splice donor site	probably null
R4660:Virma	UTSW	4	11,513,505 (GRCm39)	missense	probably damaging	1.00
R4698:Virma	UTSW	4	11,528,636 (GRCm39)	missense	probably damaging	0.99
R4878:Virma	UTSW	4	11,544,971 (GRCm39)	missense	probably damaging	1.00
R4937:Virma	UTSW	4	11,521,147 (GRCm39)	nonsense	probably null
R5031:Virma	UTSW	4	11,542,116 (GRCm39)	nonsense	probably null
R5040:Virma	UTSW	4	11,528,746 (GRCm39)	missense	probably benign	0.01
R5061:Virma	UTSW	4	11,494,840 (GRCm39)	missense	possibly damaging	0.95
R5091:Virma	UTSW	4	11,519,392 (GRCm39)	missense	probably benign	0.00
R5137:Virma	UTSW	4	11,546,297 (GRCm39)	missense	probably damaging	1.00
R5262:Virma	UTSW	4	11,539,926 (GRCm39)	missense	probably benign	0.01
R5297:Virma	UTSW	4	11,494,819 (GRCm39)	missense	probably damaging	1.00
R5730:Virma	UTSW	4	11,542,154 (GRCm39)	missense	probably benign	0.44
R5818:Virma	UTSW	4	11,513,319 (GRCm39)	missense	possibly damaging	0.92
R5835:Virma	UTSW	4	11,514,036 (GRCm39)	missense	probably damaging	1.00
R6125:Virma	UTSW	4	11,521,172 (GRCm39)	missense	probably damaging	0.98
R6197:Virma	UTSW	4	11,505,498 (GRCm39)	missense	probably damaging	0.96
R6222:Virma	UTSW	4	11,527,820 (GRCm39)	missense	probably damaging	1.00
R6793:Virma	UTSW	4	11,539,968 (GRCm39)	missense	probably damaging	1.00
R7028:Virma	UTSW	4	11,519,249 (GRCm39)	missense	possibly damaging	0.50
R7356:Virma	UTSW	4	11,513,595 (GRCm39)	missense	probably damaging	0.99
R7383:Virma	UTSW	4	11,514,026 (GRCm39)	missense	probably damaging	0.98
R7391:Virma	UTSW	4	11,508,099 (GRCm39)	missense	probably damaging	0.99
R7556:Virma	UTSW	4	11,518,927 (GRCm39)	missense	probably damaging	1.00
R7715:Virma	UTSW	4	11,513,016 (GRCm39)	splice site	probably null
R7715:Virma	UTSW	4	11,549,682 (GRCm39)	missense	probably damaging	1.00
R7986:Virma	UTSW	4	11,540,023 (GRCm39)	missense	probably benign	0.01
R7990:Virma	UTSW	4	11,513,983 (GRCm39)	missense	probably benign	0.00
R8048:Virma	UTSW	4	11,539,918 (GRCm39)	nonsense	probably null
R8050:Virma	UTSW	4	11,528,643 (GRCm39)	missense	probably benign	0.22
R8165:Virma	UTSW	4	11,542,128 (GRCm39)	missense	probably benign	0.00
R8412:Virma	UTSW	4	11,521,261 (GRCm39)	critical splice donor site	probably null
R8544:Virma	UTSW	4	11,516,949 (GRCm39)	missense	probably benign
R8551:Virma	UTSW	4	11,513,397 (GRCm39)	missense	probably damaging	1.00
R8699:Virma	UTSW	4	11,528,678 (GRCm39)	missense	probably benign	0.04
R8739:Virma	UTSW	4	11,540,643 (GRCm39)	critical splice donor site	probably null
R8950:Virma	UTSW	4	11,519,047 (GRCm39)	nonsense	probably null
R9015:Virma	UTSW	4	11,540,494 (GRCm39)	missense	probably benign	0.27
R9038:Virma	UTSW	4	11,526,922 (GRCm39)	missense	possibly damaging	0.93
R9115:Virma	UTSW	4	11,498,744 (GRCm39)	missense	probably benign	0.15
R9294:Virma	UTSW	4	11,513,507 (GRCm39)	nonsense	probably null
R9404:Virma	UTSW	4	11,513,626 (GRCm39)	missense	probably benign	0.17
R9477:Virma	UTSW	4	11,528,753 (GRCm39)	missense	probably damaging	1.00
R9532:Virma	UTSW	4	11,507,078 (GRCm39)	critical splice donor site	probably null
R9649:Virma	UTSW	4	11,486,045 (GRCm39)	start codon destroyed	probably null	0.08
R9657:Virma	UTSW	4	11,544,898 (GRCm39)	missense	probably damaging	0.99
R9780:Virma	UTSW	4	11,513,442 (GRCm39)	missense	possibly damaging	0.75
R9800:Virma	UTSW	4	11,546,007 (GRCm39)	missense	probably damaging	0.99
X0020:Virma	UTSW	4	11,486,055 (GRCm39)	missense	probably benign	0.00

Predicted Primers

PCR Primer
(F):5'- CATTGAACCTGCCAAACGAG -3'
(R):5'- AAGTCCCTGGGCCCATTCTATC -3'

Sequencing Primer
(F):5'- TGCCAAACGAGCTCATGTTG -3'
(R):5'- ATCTGGGTCCCGTGTTTACTAC -3'

Posted On

2019-10-07