Incidental Mutation 'R7440:Cry2'
ID 576870
Institutional Source Beutler Lab
Gene Symbol Cry2
Ensembl Gene ENSMUSG00000068742
Gene Name cryptochrome circadian regulator 2
Synonyms D130054K12Rik
MMRRC Submission 045516-MU
Accession Numbers
Essential gene? Possibly essential (E-score: 0.579) question?
Stock # R7440 (G1)
Quality Score 225.009
Status Validated
Chromosome 2
Chromosomal Location 92233991-92264388 bp(-) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) G to A at 92243983 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Arginine to Tryptophan at position 397 (R397W)
Ref Sequence ENSEMBL: ENSMUSP00000088047 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000090559] [ENSMUST00000111278]
AlphaFold Q9R194
PDB Structure A vertebrate cryptochrome [X-RAY DIFFRACTION]
a vertebrate cryptochrome with FAD [X-RAY DIFFRACTION]
A ubiquitin ligase-substrate complex [X-RAY DIFFRACTION]
Mammalian cryptochrome in complex with a small molecule competitor of its ubiquitin ligase [X-RAY DIFFRACTION]
Crystal Structure of Mammalian Period-Cryptochrome Complex [X-RAY DIFFRACTION]
Predicted Effect probably damaging
Transcript: ENSMUST00000090559
AA Change: R397W

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)
SMART Domains Protein: ENSMUSP00000088047
Gene: ENSMUSG00000068742
AA Change: R397W

DomainStartEndE-ValueType
low complexity region 2 13 N/A INTRINSIC
Pfam:DNA_photolyase 23 187 2.4e-50 PFAM
Pfam:FAD_binding_7 231 504 4.4e-89 PFAM
low complexity region 562 573 N/A INTRINSIC
Predicted Effect probably damaging
Transcript: ENSMUST00000111278
AA Change: R397W

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)
SMART Domains Protein: ENSMUSP00000106909
Gene: ENSMUSG00000068742
AA Change: R397W

DomainStartEndE-ValueType
low complexity region 2 13 N/A INTRINSIC
Pfam:DNA_photolyase 23 189 3.6e-50 PFAM
Pfam:FAD_binding_7 230 506 1.4e-105 PFAM
low complexity region 562 573 N/A INTRINSIC
Meta Mutation Damage Score 0.2118 question?
Coding Region Coverage
  • 1x: 100.0%
  • 3x: 100.0%
  • 10x: 99.7%
  • 20x: 98.9%
Validation Efficiency 100% (69/69)
MGI Phenotype FUNCTION: This gene encodes a flavin adenine dinucleotide-binding protein that is a key component of the circadian core oscillator complex, which regulates the circadian clock. This gene is upregulated by Clock/Arntl heterodimers but then represses this upregulation in a feedback loop using Per/Cry heterodimers to interact with Clock/Arntl. Polymorphisms in this gene have been associated with altered sleep patterns. The encoded protein is widely conserved across plants and animals. [provided by RefSeq, Feb 2014]
PHENOTYPE: Homozygotes for targeted null mutations exhibit a one-hour longer circadian period under constant darkness, and reduced expression of another circadian gene in the suprachiasmatic nucleus in response to acute light exposure. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 69 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Acadm G A 3: 153,628,626 (GRCm39) T403I probably damaging Het
Adat1 A T 8: 112,716,530 (GRCm39) M64K probably damaging Het
Adcy2 C T 13: 68,944,786 (GRCm39) V199M probably damaging Het
Ankrd13a T C 5: 114,941,636 (GRCm39) S508P possibly damaging Het
Ap1g1 T A 8: 110,529,356 (GRCm39) probably null Het
Bicral C T 17: 47,136,710 (GRCm39) G167R probably damaging Het
Ccser2 T C 14: 36,620,174 (GRCm39) K727E possibly damaging Het
Cep350 T G 1: 155,816,518 (GRCm39) K332N probably damaging Het
Cfap68 A G 9: 50,676,213 (GRCm39) V35A probably benign Het
Chd5 A G 4: 152,469,108 (GRCm39) N1811S probably benign Het
Chpf A T 1: 75,452,245 (GRCm39) V565D probably damaging Het
Clcn6 A T 4: 148,098,652 (GRCm39) L489H probably damaging Het
Cntln C A 4: 84,981,453 (GRCm39) T877K possibly damaging Het
Cog4 T C 8: 111,606,338 (GRCm39) V630A probably benign Het
Col6a5 G T 9: 105,758,630 (GRCm39) S2192* probably null Het
Cstdc2 A C 2: 148,688,911 (GRCm39) C109W probably damaging Het
Cyp1b1 T C 17: 80,020,986 (GRCm39) N252S probably damaging Het
Dhx9 T C 1: 153,356,977 (GRCm39) I91V probably benign Het
Dnajb6 G A 5: 29,962,857 (GRCm39) A256T possibly damaging Het
Epm2a T G 10: 11,266,619 (GRCm39) Y121* probably null Het
Erlec1 T C 11: 30,900,818 (GRCm39) I117V possibly damaging Het
Exoc8 T C 8: 125,622,520 (GRCm39) M616V probably benign Het
Fmn1 A T 2: 113,271,956 (GRCm39) Q108L unknown Het
Fuz T C 7: 44,545,996 (GRCm39) L46P probably damaging Het
Insc T C 7: 114,444,278 (GRCm39) S422P possibly damaging Het
Intu A G 3: 40,651,981 (GRCm39) I813V probably benign Het
Jak3 T A 8: 72,133,362 (GRCm39) S352T probably benign Het
Klf11 T C 12: 24,705,490 (GRCm39) S315P probably benign Het
Lrrc63 T C 14: 75,358,453 (GRCm39) N400D possibly damaging Het
Lrrk1 T C 7: 65,940,602 (GRCm39) D760G probably damaging Het
Macf1 A T 4: 123,349,239 (GRCm39) L3976* probably null Het
Map7 C T 10: 20,137,605 (GRCm39) A259V probably damaging Het
Meioc T A 11: 102,565,063 (GRCm39) D170E possibly damaging Het
Mgat5b T A 11: 116,859,271 (GRCm39) Y34* probably null Het
Mgst1 A G 6: 138,127,842 (GRCm39) K68R probably benign Het
Miga1 T A 3: 152,043,683 (GRCm39) probably null Het
Mrps33 G A 6: 39,779,413 (GRCm39) P94L probably damaging Het
Ncapg2 G T 12: 116,414,033 (GRCm39) G1068C possibly damaging Het
Ndufaf7 C T 17: 79,249,546 (GRCm39) H148Y probably damaging Het
Nkx6-3 A T 8: 23,643,770 (GRCm39) D57V probably damaging Het
Nlrp1a T C 11: 70,983,150 (GRCm39) D1272G probably damaging Het
Oit3 T C 10: 59,265,392 (GRCm39) N291S probably damaging Het
Or1b1 A T 2: 36,995,181 (GRCm39) H160Q possibly damaging Het
Pld1 T G 3: 28,095,419 (GRCm39) S251A probably benign Het
Polr1h T A 17: 37,268,736 (GRCm39) L75F probably benign Het
Potefam3a C T 8: 20,356,948 (GRCm38) S254N unknown Het
Ppfibp1 T C 6: 146,921,001 (GRCm39) S580P probably benign Het
Prdx6b A T 2: 80,123,560 (GRCm39) D123V probably damaging Het
Ptprs A G 17: 56,731,256 (GRCm39) L1050P possibly damaging Het
Rcc1 A C 4: 132,065,110 (GRCm39) S138A probably damaging Het
Rimbp3 G T 16: 17,031,065 (GRCm39) R1496S possibly damaging Het
Sacs T C 14: 61,429,054 (GRCm39) V371A probably benign Het
Sfxn4 A G 19: 60,830,642 (GRCm39) L260P possibly damaging Het
Slc17a1 G A 13: 24,062,466 (GRCm39) S211N possibly damaging Het
Slc39a11 C T 11: 113,452,918 (GRCm39) V8M probably damaging Het
Smpd2 A G 10: 41,365,012 (GRCm39) I78T probably benign Het
Steap3 A C 1: 120,169,248 (GRCm39) F350V probably benign Het
Syt17 A T 7: 117,981,107 (GRCm39) V462E probably damaging Het
Tlr11 T A 14: 50,598,801 (GRCm39) D262E probably benign Het
Tnfrsf9 T C 4: 151,014,331 (GRCm39) V10A probably benign Het
Trpv3 C A 11: 73,168,800 (GRCm39) Q87K probably benign Het
Ugt1a5 T C 1: 88,094,281 (GRCm39) Y170H probably benign Het
Urb1 A G 16: 90,584,296 (GRCm39) L562P probably damaging Het
Usp3 G T 9: 66,437,537 (GRCm39) N299K probably benign Het
Vmn1r83 T C 7: 12,055,556 (GRCm39) Y167C probably damaging Het
Vmn2r117 T A 17: 23,694,539 (GRCm39) Y436F probably benign Het
Vps13d A G 4: 144,854,981 (GRCm39) I2220T Het
Zfp936 T A 7: 42,836,685 (GRCm39) V32D probably damaging Het
Zswim2 A G 2: 83,751,063 (GRCm39) C259R probably damaging Het
Other mutations in Cry2
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01995:Cry2 APN 2 92,254,977 (GRCm39) missense probably benign 0.15
IGL02167:Cry2 APN 2 92,264,166 (GRCm39) missense possibly damaging 0.93
IGL02183:Cry2 APN 2 92,243,384 (GRCm39) missense probably damaging 0.99
IGL02343:Cry2 APN 2 92,257,266 (GRCm39) missense possibly damaging 0.90
IGL02432:Cry2 APN 2 92,244,012 (GRCm39) missense probably damaging 0.99
IGL02725:Cry2 APN 2 92,243,605 (GRCm39) splice site probably benign
IGL02932:Cry2 APN 2 92,243,462 (GRCm39) nonsense probably null
IGL03122:Cry2 APN 2 92,243,640 (GRCm39) missense probably damaging 1.00
IGL03366:Cry2 APN 2 92,244,060 (GRCm39) missense probably damaging 1.00
R0679:Cry2 UTSW 2 92,244,060 (GRCm39) missense probably damaging 1.00
R1325:Cry2 UTSW 2 92,244,115 (GRCm39) missense probably damaging 1.00
R1862:Cry2 UTSW 2 92,254,911 (GRCm39) missense probably damaging 1.00
R1891:Cry2 UTSW 2 92,243,985 (GRCm39) missense possibly damaging 0.93
R2189:Cry2 UTSW 2 92,242,037 (GRCm39) missense possibly damaging 0.84
R4032:Cry2 UTSW 2 92,244,172 (GRCm39) missense probably benign 0.00
R4689:Cry2 UTSW 2 92,254,899 (GRCm39) missense probably benign 0.38
R5130:Cry2 UTSW 2 92,254,944 (GRCm39) missense probably benign 0.28
R5145:Cry2 UTSW 2 92,243,405 (GRCm39) missense probably benign
R5970:Cry2 UTSW 2 92,243,312 (GRCm39) missense probably benign 0.08
R6179:Cry2 UTSW 2 92,244,187 (GRCm39) missense probably damaging 0.98
R7102:Cry2 UTSW 2 92,243,438 (GRCm39) missense probably damaging 0.99
R7158:Cry2 UTSW 2 92,244,060 (GRCm39) missense probably damaging 1.00
R7213:Cry2 UTSW 2 92,244,004 (GRCm39) missense probably benign 0.00
R7257:Cry2 UTSW 2 92,243,326 (GRCm39) missense possibly damaging 0.67
R7378:Cry2 UTSW 2 92,244,009 (GRCm39) missense probably damaging 1.00
R7427:Cry2 UTSW 2 92,243,392 (GRCm39) missense possibly damaging 0.74
R7428:Cry2 UTSW 2 92,243,392 (GRCm39) missense possibly damaging 0.74
R7531:Cry2 UTSW 2 92,243,350 (GRCm39) missense probably damaging 0.98
R8234:Cry2 UTSW 2 92,242,974 (GRCm39) missense probably benign
R8350:Cry2 UTSW 2 92,244,286 (GRCm39) missense probably benign 0.00
R8450:Cry2 UTSW 2 92,244,286 (GRCm39) missense probably benign 0.00
R8496:Cry2 UTSW 2 92,257,284 (GRCm39) missense probably damaging 1.00
R9172:Cry2 UTSW 2 92,243,993 (GRCm39) missense probably damaging 1.00
R9283:Cry2 UTSW 2 92,244,249 (GRCm39) missense probably damaging 1.00
Predicted Primers PCR Primer
(F):5'- AAACCAGCCTCCTTGGAGAC -3'
(R):5'- CCAGGTTTGACCGAATGGAG -3'

Sequencing Primer
(F):5'- TTGGAGACCTGACGCTCTG -3'
(R):5'- TTTGACCGAATGGAGGGGAACC -3'
Posted On 2019-10-07