Mutagenetix > Incidental Mutation

Incidental Mutation 'R7441:Zfp810'

576957

Institutional Source

Beutler Lab

Gene Symbol

Zfp810

Ensembl Gene

ENSMUSG00000066829

Gene Name

zinc finger protein 810

Synonyms

MMRRC Submission

045517-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.093) question?

Stock #

R7441 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

22188044-22218944 bp(-) (GRCm39)

Type of Mutation

nonsense

DNA Base Change (assembly)

C to A at 22190568 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Glutamic Acid to Stop codon at position 78 (E78*)

Ref Sequence

ENSEMBL: ENSMUSP00000150816 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000086278] [ENSMUST00000215202]

AlphaFold

Q99K45

Predicted Effect

silent
Transcript: ENSMUST00000086278

SMART Domains

Protein: ENSMUSP00000083459
Gene: ENSMUSG00000066829

Domain	Start	End	E-Value	Type
KRAB	4	64	1.09e-33	SMART
ZnF_C2H2	126	148	2.44e2	SMART
ZnF_C2H2	182	204	3.07e-1	SMART
ZnF_C2H2	210	232	8.47e-4	SMART
ZnF_C2H2	238	260	6.78e-3	SMART
ZnF_C2H2	266	288	6.13e-1	SMART
ZnF_C2H2	294	316	5.06e-2	SMART
ZnF_C2H2	322	344	4.79e-3	SMART
ZnF_C2H2	350	372	2.99e-4	SMART
ZnF_C2H2	378	400	1.33e-1	SMART
ZnF_C2H2	406	428	2.75e-3	SMART
ZnF_C2H2	434	456	1.58e-3	SMART

Predicted Effect

probably null
Transcript: ENSMUST00000215202
AA Change: E78*

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.7%
20x: 99.0%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 56 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4921509C19Rik	A	C	2: 151,314,845 (GRCm39)	S278A	possibly damaging	Het
Adamts20	T	C	15: 94,251,554 (GRCm39)	D411G	probably damaging	Het
Adgrl3	A	G	5: 81,871,987 (GRCm39)	I894V	possibly damaging	Het
Adprhl1	C	T	8: 13,273,069 (GRCm39)	V1230I	probably benign	Het
Agpat1	T	A	17: 34,829,883 (GRCm39)	Y77N	probably damaging	Het
Anpep	A	G	7: 79,477,392 (GRCm39)	V725A	possibly damaging	Het
Apc	T	A	18: 34,445,126 (GRCm39)	I674K	probably damaging	Het
Arhgef2	A	G	3: 88,551,262 (GRCm39)	R808G	probably damaging	Het
Asap1	A	G	15: 64,002,105 (GRCm39)	V402A	probably damaging	Het
Aspg	T	C	12: 112,091,255 (GRCm39)	V479A	possibly damaging	Het
B3galnt2	G	A	13: 14,169,070 (GRCm39)	V368M	probably benign	Het
Bahcc1	T	C	11: 120,177,132 (GRCm39)	S2007P	probably damaging	Het
Cyfip2	A	T	11: 46,087,254 (GRCm39)	I1212N	possibly damaging	Het
Dnajc16	A	T	4: 141,491,124 (GRCm39)	D675E	probably damaging	Het
Dram2	T	C	3: 106,462,503 (GRCm39)	F4L	probably damaging	Het
Dsp	A	G	13: 38,379,425 (GRCm39)	T2057A	probably benign	Het
Dync1h1	T	G	12: 110,602,887 (GRCm39)	L2176R	probably damaging	Het
Efhb	A	T	17: 53,708,549 (GRCm39)	I707N	possibly damaging	Het
Eif2ak4	A	G	2: 118,302,377 (GRCm39)	T1555A	probably benign	Het
Erc1	G	A	6: 119,801,912 (GRCm39)	T35I	possibly damaging	Het
Esr2	C	A	12: 76,188,168 (GRCm39)	M363I	probably benign	Het
Etl4	A	T	2: 20,749,000 (GRCm39)	N446I	possibly damaging	Het
Evpl	T	A	11: 116,113,782 (GRCm39)	K1303*	probably null	Het
Fam135b	A	T	15: 71,335,529 (GRCm39)	V555E	probably damaging	Het
Fam186b	A	G	15: 99,177,970 (GRCm39)	L452P	probably benign	Het
Fmn1	A	T	2: 113,271,956 (GRCm39)	Q108L	unknown	Het
Gcc2	A	G	10: 58,092,723 (GRCm39)	T48A	probably benign	Het
Gm6619	T	G	6: 131,467,354 (GRCm39)	I73S	possibly damaging	Het
Gm8267	T	A	14: 44,960,397 (GRCm39)	D116V	probably damaging	Het
Gtf3c3	G	A	1: 54,459,607 (GRCm39)	T385M	probably benign	Het
Iqgap2	C	A	13: 95,764,584 (GRCm39)	M1553I	probably benign	Het
Kcnk1	G	T	8: 126,722,307 (GRCm39)	G37C	probably damaging	Het
Kifc3	T	C	8: 95,864,615 (GRCm39)	M32V	probably benign	Het
Krt81	C	A	15: 101,359,251 (GRCm39)	K222N	possibly damaging	Het
Lrrc63	A	G	14: 75,363,697 (GRCm39)	S145P	possibly damaging	Het
Mybbp1a	T	A	11: 72,342,101 (GRCm39)	V1279E	probably benign	Het
Niban3	A	G	8: 72,052,808 (GRCm39)	D94G	probably benign	Het
Or11g26	A	T	14: 50,752,853 (GRCm39)	Y64F	probably damaging	Het
Or8u9	T	A	2: 86,001,354 (GRCm39)	D269V	probably damaging	Het
Pierce1	TCTCTGGGGCAGGCTTAGCCTTGGGCTCCCCCGGCTCCGGCTCCTCTGGGGCAGGCTTAGCCTTGGGCTCCCCCGGCTCCGGCTCCTCTGGGGCGGGCTTAGCCTTGGGCTCCCCCGGCTCCGGCTCCTC	TCTCTGGGGCAGGCTTAGCCTTGGGCTCCCCCGGCTCCGGCTCCTCTGGGGCGGGCTTAGCCTTGGGCTCCCCCGGCTCCGGCTCCTC	2: 28,356,122 (GRCm39)		probably benign	Het
Pramel12	A	G	4: 143,145,410 (GRCm39)	Y293C	probably benign	Het
Ptpn18	T	C	1: 34,512,416 (GRCm39)	V407A	probably benign	Het
Ptpn9	T	A	9: 56,934,717 (GRCm39)	Y160*	probably null	Het
Ptprj	T	C	2: 90,280,163 (GRCm39)	K1045R	possibly damaging	Het
Rundc3a	G	T	11: 102,290,872 (GRCm39)		probably null	Het
Scn11a	C	T	9: 119,587,692 (GRCm39)	V1351I	probably benign	Het
Slc26a9	A	G	1: 131,690,556 (GRCm39)	Y520C	probably damaging	Het
Spata20	G	A	11: 94,374,867 (GRCm39)	A245V	probably benign	Het
Steap3	A	C	1: 120,169,248 (GRCm39)	F350V	probably benign	Het
Swt1	A	T	1: 151,286,815 (GRCm39)	F226I	probably benign	Het
Taar7f	A	G	10: 23,925,885 (GRCm39)	T160A	possibly damaging	Het
Timd5	G	A	11: 46,419,382 (GRCm39)	W66*	probably null	Het
Upf2	A	T	2: 6,023,743 (GRCm39)	I698F	unknown	Het
Vmn2r84	T	C	10: 130,227,982 (GRCm39)	T85A	possibly damaging	Het
Zfp426	T	C	9: 20,382,147 (GRCm39)	E280G	possibly damaging	Het
Zfp937	GTGATAAGGCATTTGCACAAAACAGTCATCTCCTAACACATAAAAGAACACAT	G	2: 150,080,630 (GRCm39)		probably null	Het

Other mutations in Zfp810

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00484:Zfp810	APN	9	22,189,605 (GRCm39)	nonsense	probably null
IGL03079:Zfp810	APN	9	22,195,423 (GRCm39)	missense	probably damaging	1.00
IGL03402:Zfp810	APN	9	22,190,441 (GRCm39)	splice site	probably null
H8562:Zfp810	UTSW	9	22,190,387 (GRCm39)	missense	probably benign	0.42
R1116:Zfp810	UTSW	9	22,190,381 (GRCm39)	missense	probably benign	0.11
R1160:Zfp810	UTSW	9	22,189,828 (GRCm39)	missense	possibly damaging	0.64
R1171:Zfp810	UTSW	9	22,190,122 (GRCm39)	missense	possibly damaging	0.95
R1393:Zfp810	UTSW	9	22,191,810 (GRCm39)	missense	probably benign
R1608:Zfp810	UTSW	9	22,190,216 (GRCm39)	missense	probably benign	0.00
R1644:Zfp810	UTSW	9	22,190,324 (GRCm39)	missense	possibly damaging	0.67
R1766:Zfp810	UTSW	9	22,189,828 (GRCm39)	missense	possibly damaging	0.64
R2568:Zfp810	UTSW	9	22,190,534 (GRCm39)	missense	probably benign	0.01
R3684:Zfp810	UTSW	9	22,189,531 (GRCm39)	missense	probably benign	0.01
R4002:Zfp810	UTSW	9	22,190,188 (GRCm39)	missense	probably damaging	1.00
R4134:Zfp810	UTSW	9	22,190,369 (GRCm39)	missense	probably damaging	0.97
R4135:Zfp810	UTSW	9	22,190,369 (GRCm39)	missense	probably damaging	0.97
R4334:Zfp810	UTSW	9	22,190,080 (GRCm39)	missense	probably benign	0.00
R4545:Zfp810	UTSW	9	22,190,041 (GRCm39)	missense	probably damaging	0.96
R5399:Zfp810	UTSW	9	22,190,125 (GRCm39)	missense	possibly damaging	0.91
R5622:Zfp810	UTSW	9	22,190,392 (GRCm39)	missense	probably benign	0.00
R5643:Zfp810	UTSW	9	22,194,467 (GRCm39)	missense	probably benign	0.26
R7375:Zfp810	UTSW	9	22,201,833 (GRCm39)	critical splice donor site	probably null
R7809:Zfp810	UTSW	9	22,190,278 (GRCm39)	missense	possibly damaging	0.51
R8422:Zfp810	UTSW	9	22,194,518 (GRCm39)	nonsense	probably null
R8526:Zfp810	UTSW	9	22,189,586 (GRCm39)	missense	probably damaging	1.00
R8719:Zfp810	UTSW	9	22,190,571 (GRCm39)	missense	probably benign	0.00
R9177:Zfp810	UTSW	9	22,189,936 (GRCm39)	missense	probably damaging	1.00
R9479:Zfp810	UTSW	9	22,194,497 (GRCm39)	missense	possibly damaging	0.68
R9521:Zfp810	UTSW	9	22,190,227 (GRCm39)	missense	possibly damaging	0.81
R9683:Zfp810	UTSW	9	22,189,799 (GRCm39)	missense	possibly damaging	0.90

Predicted Primers

PCR Primer
(F):5'- AGCCTCTGATACTGACTGAGGG -3'
(R):5'- GCAGATCAGGAAGCAGAGTTCC -3'

Sequencing Primer
(F):5'- CCTCTGATACTGACTGAGGGTAGAC -3'
(R):5'- TCAGGAAGCAGAGTTCCTAATAC -3'

Posted On

2019-10-07