Mutagenetix > Incidental Mutation

Incidental Mutation 'R7448:Magi2'

577454

Institutional Source

Beutler Lab

Gene Symbol

Magi2

Ensembl Gene

ENSMUSG00000040003

Gene Name

membrane associated guanylate kinase, WW and PDZ domain containing 2

Synonyms

Acvrinp1, Magi-2, S-SCAM

MMRRC Submission

045523-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R7448 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

19432034-20909790 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

G to A at 20563954 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Glycine to Aspartic acid at position 199 (G199D)

Ref Sequence

ENSEMBL: ENSMUSP00000099094 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000088516] [ENSMUST00000101558] [ENSMUST00000115267] [ENSMUST00000197354] [ENSMUST00000197443] [ENSMUST00000197553] [ENSMUST00000208219]

AlphaFold

Q9WVQ1

Predicted Effect

probably damaging
Transcript: ENSMUST00000088516
AA Change: G362D

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000085872
Gene: ENSMUSG00000040003
AA Change: G362D

Domain	Start	End	E-Value	Type
PDZ	26	101	5.26e-9	SMART
GuKc	107	290	2.76e-45	SMART
WW	302	334	7.43e-12	SMART
WW	348	380	2.4e-6	SMART
PDZ	433	509	3.51e-19	SMART
PDZ	612	682	2.3e-14	SMART
PDZ	785	861	4.04e-19	SMART
low complexity region	893	907	N/A	INTRINSIC
PDZ	928	1009	5.05e-20	SMART
low complexity region	1052	1063	N/A	INTRINSIC
PDZ	1147	1221	3.88e-21	SMART
low complexity region	1257	1270	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000101558
AA Change: G199D

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000099094
Gene: ENSMUSG00000040003
AA Change: G199D

Domain	Start	End	E-Value	Type
low complexity region	66	74	N/A	INTRINSIC
WW	139	171	7.43e-12	SMART
WW	185	217	2.4e-6	SMART
PDZ	270	346	3.51e-19	SMART
PDZ	449	519	2.3e-14	SMART
PDZ	608	684	4.04e-19	SMART
low complexity region	716	730	N/A	INTRINSIC
PDZ	751	832	5.05e-20	SMART
low complexity region	875	886	N/A	INTRINSIC
PDZ	970	1044	3.88e-21	SMART
low complexity region	1080	1093	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000115267
AA Change: G199D

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000110922
Gene: ENSMUSG00000040003
AA Change: G199D

Domain	Start	End	E-Value	Type
low complexity region	66	74	N/A	INTRINSIC
WW	139	171	7.43e-12	SMART
WW	185	217	2.4e-6	SMART
PDZ	270	346	3.51e-19	SMART
PDZ	449	519	2.3e-14	SMART
PDZ	622	698	4.04e-19	SMART
low complexity region	730	744	N/A	INTRINSIC
PDZ	765	846	5.05e-20	SMART
low complexity region	889	900	N/A	INTRINSIC
PDZ	984	1058	3.88e-21	SMART
low complexity region	1094	1107	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000197354
AA Change: G362D

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000142576
Gene: ENSMUSG00000040003
AA Change: G362D

Domain	Start	End	E-Value	Type
PDZ	26	101	2.5e-11	SMART
GuKc	107	290	1.4e-47	SMART
WW	302	334	4.3e-14	SMART
WW	348	380	1.4e-8	SMART
PDZ	433	509	1.7e-21	SMART
PDZ	612	682	1.1e-16	SMART
PDZ	785	861	2e-21	SMART
low complexity region	893	907	N/A	INTRINSIC
PDZ	928	1009	2.4e-22	SMART
low complexity region	1052	1063	N/A	INTRINSIC
PDZ	1147	1221	1.9e-23	SMART
low complexity region	1255	1269	N/A	INTRINSIC
low complexity region	1304	1319	N/A	INTRINSIC
low complexity region	1344	1363	N/A	INTRINSIC
low complexity region	1368	1384	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000197443
AA Change: G362D

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000142764
Gene: ENSMUSG00000040003
AA Change: G362D

Domain	Start	End	E-Value	Type
PDZ	26	101	2.5e-11	SMART
GuKc	107	290	1.4e-47	SMART
WW	302	334	4.3e-14	SMART
WW	348	380	1.4e-8	SMART
PDZ	433	509	1.7e-21	SMART
PDZ	612	682	1.1e-16	SMART
PDZ	771	847	2e-21	SMART
low complexity region	879	893	N/A	INTRINSIC
PDZ	914	995	2.4e-22	SMART
low complexity region	1038	1049	N/A	INTRINSIC
PDZ	1133	1207	1.9e-23	SMART
low complexity region	1241	1255	N/A	INTRINSIC
low complexity region	1290	1305	N/A	INTRINSIC
low complexity region	1330	1349	N/A	INTRINSIC
low complexity region	1354	1370	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000197553

Predicted Effect

probably benign
Transcript: ENSMUST00000208219

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.7%
20x: 98.9%

Validation Efficiency

100% (117/117)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene interacts with atrophin-1. Atrophin-1 contains a polyglutamine repeat, expansion of which is responsible for dentatorubral and pallidoluysian atrophy. This encoded protein is characterized by two WW domains, a guanylate kinase-like domain, and multiple PDZ domains. It has structural similarity to the membrane-associated guanylate kinase homologue (MAGUK) family. [provided by RefSeq, Jul 2008]
PHENOTYPE: Homozygotes for a null allele show neonatal death and hippocampal neurons with altered dendritic spine morphology. Homozygotes for a different null allele die neonatally due to anuria and podocyte anomalies. Mice lacking all three isoforms develop proteinuria, podocytopathy and die of renal failure. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 119 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Acss2	T	C	2: 155,360,186 (GRCm39)	S54P	probably damaging	Het
Ahnak2	T	C	12: 112,746,605 (GRCm39)	K1242E		Het
Alpi	T	A	1: 87,029,257 (GRCm39)	M1L	possibly damaging	Het
Atp2c1	C	T	9: 105,329,982 (GRCm39)	A283T	probably damaging	Het
Atp8b5	T	G	4: 43,366,021 (GRCm39)	M764R	probably benign	Het
B4galnt2	T	A	11: 95,760,193 (GRCm39)	H278L	probably damaging	Het
Bcl9l	G	T	9: 44,420,634 (GRCm39)	A1347S	probably benign	Het
Bicd2	A	G	13: 49,533,427 (GRCm39)	E671G	probably damaging	Het
Bmp3	A	T	5: 99,020,077 (GRCm39)	I167F	probably damaging	Het
Bpifb5	T	A	2: 154,072,105 (GRCm39)	C271S	possibly damaging	Het
Camsap2	T	A	1: 136,198,644 (GRCm39)	H793L		Het
Casp8ap2	T	A	4: 32,643,974 (GRCm39)	S1016T	possibly damaging	Het
Castor1	A	G	11: 4,171,897 (GRCm39)	S325G	not run	Het
Ccdc134	T	A	15: 82,025,149 (GRCm39)	I216N	possibly damaging	Het
Ccdc63	G	T	5: 122,246,245 (GRCm39)	R559S	probably benign	Het
Cd276	T	C	9: 58,442,895 (GRCm39)	T187A	probably benign	Het
Ciao1	C	T	2: 127,087,678 (GRCm39)	R219H	probably damaging	Het
Clmn	T	C	12: 104,751,687 (GRCm39)	D256G	possibly damaging	Het
Cobl	A	C	11: 12,206,225 (GRCm39)	M550R	possibly damaging	Het
Cracr2b	A	T	7: 141,044,118 (GRCm39)	T117S	probably benign	Het
Cx3cr1	G	A	9: 119,881,282 (GRCm39)	A40V	probably benign	Het
Cxcl14	A	G	13: 56,440,344 (GRCm39)	C72R	probably damaging	Het
Dab2	T	A	15: 6,451,747 (GRCm39)	I121N	probably damaging	Het
Dapk1	A	G	13: 60,898,990 (GRCm39)	Y820C	probably damaging	Het
Dele1	A	G	18: 38,390,319 (GRCm39)	N256D	probably damaging	Het
Ech1	T	A	7: 28,525,623 (GRCm39)	C91S	probably damaging	Het
Exosc5	T	G	7: 25,358,734 (GRCm39)	V25G	probably benign	Het
Fbp1	A	C	13: 63,020,564 (GRCm39)	D122E	possibly damaging	Het
Fbxw13	A	G	9: 109,014,471 (GRCm39)	Y101H	unknown	Het
Fcsk	C	T	8: 111,616,963 (GRCm39)	G396S	possibly damaging	Het
Fhip1a	G	A	3: 85,579,871 (GRCm39)	S778L	probably benign	Het
Fmnl1	T	A	11: 103,077,453 (GRCm39)	V271E	probably damaging	Het
Galnt1	T	A	18: 24,417,866 (GRCm39)	S545T	probably benign	Het
Galnt13	G	A	2: 54,406,576 (GRCm39)	V9M	possibly damaging	Het
Gm7995	A	T	14: 42,132,302 (GRCm39)	I45F		Het
Gpr137	C	T	19: 6,917,726 (GRCm39)	R134Q	possibly damaging	Het
Gpr22	T	G	12: 31,759,514 (GRCm39)	I203L	probably benign	Het
H2-Q10	T	C	17: 35,784,457 (GRCm39)	Y324H	not run	Het
Hcn4	G	A	9: 58,751,582 (GRCm39)	E403K	unknown	Het
Hddc2	T	A	10: 31,189,412 (GRCm39)	M1K	probably null	Het
Hps3	A	G	3: 20,089,329 (GRCm39)	F34S	probably damaging	Het
Ift56	T	A	6: 38,381,422 (GRCm39)	Y319*	probably null	Het
Igdcc4	G	T	9: 65,031,276 (GRCm39)	V405L	possibly damaging	Het
Itpr2	C	A	6: 146,231,006 (GRCm39)	V1215L	probably damaging	Het
Kif26b	T	C	1: 178,742,339 (GRCm39)	S812P	probably damaging	Het
Lgi1	T	A	19: 38,289,713 (GRCm39)	C260S	probably damaging	Het
Lhfpl6	A	G	3: 53,168,020 (GRCm39)	Y198C	probably damaging	Het
Lrp5	T	C	19: 3,699,439 (GRCm39)	D282G	probably benign	Het
Lrpprc	T	C	17: 85,079,567 (GRCm39)	T230A	probably damaging	Het
Lrtm2	A	G	6: 119,297,784 (GRCm39)	W86R	probably benign	Het
Maco1	A	T	4: 134,555,590 (GRCm39)	N294K	possibly damaging	Het
Map1b	C	T	13: 99,644,648 (GRCm39)	R85Q	probably damaging	Het
Marchf7	T	A	2: 60,077,858 (GRCm39)		probably null	Het
Morc1	A	G	16: 48,251,708 (GRCm39)	D2G	probably damaging	Het
Mpp7	A	T	18: 7,351,079 (GRCm39)	F539L	probably damaging	Het
Muc13	A	T	16: 33,634,951 (GRCm39)	I502F	probably damaging	Het
Myh13	A	G	11: 67,255,286 (GRCm39)		probably null	Het
Nat10	C	G	2: 103,578,390 (GRCm39)	L238F	probably damaging	Het
Nckap1	G	A	2: 80,354,885 (GRCm39)	T679I	probably damaging	Het
Npy6r	T	A	18: 44,409,260 (GRCm39)	I227N	probably damaging	Het
Nudt18	A	T	14: 70,815,389 (GRCm39)	M1L	unknown	Het
Or13d1	T	A	4: 52,971,207 (GRCm39)	N195K	probably damaging	Het
Or14a257	G	A	7: 86,138,417 (GRCm39)	T114I	probably damaging	Het
Or4f14	T	A	2: 111,742,637 (GRCm39)	I213L	probably benign	Het
Or8d1	C	A	9: 38,766,412 (GRCm39)	T18K	probably damaging	Het
Or8u8	T	C	2: 86,011,678 (GRCm39)	Y259C	probably damaging	Het
Pcdha3	T	A	18: 37,079,266 (GRCm39)	F3I	probably benign	Het
Pcdhga3	T	A	18: 37,808,917 (GRCm39)	Y457N	possibly damaging	Het
Pclo	A	T	5: 14,719,631 (GRCm39)	Q1256L	unknown	Het
Piezo2	C	T	18: 63,157,543 (GRCm39)	R2389H	probably damaging	Het
Pml	G	T	9: 58,154,496 (GRCm39)	Q126K	probably benign	Het
Ppef2	A	G	5: 92,376,563 (GRCm39)	Y655H	probably damaging	Het
Ppp4r1	T	C	17: 66,147,936 (GRCm39)	V926A	probably damaging	Het
Psg29	A	G	7: 16,945,648 (GRCm39)	D406G	possibly damaging	Het
Ptprf	T	C	4: 118,092,864 (GRCm39)	D517G	probably benign	Het
Ptprg	G	A	14: 12,142,461 (GRCm38)	E371K	probably benign	Het
Pttg1ip2	T	C	5: 5,505,952 (GRCm39)	I110V	probably benign	Het
Rasgrp1	T	C	2: 117,118,424 (GRCm39)	I522V	probably damaging	Het
Rasgrp1	T	A	2: 117,122,178 (GRCm39)	D404V	possibly damaging	Het
Rb1cc1	T	A	1: 6,315,727 (GRCm39)	F541I	probably damaging	Het
Rgsl1	C	A	1: 153,719,847 (GRCm39)		probably null	Het
Rhobtb2	C	T	14: 70,033,397 (GRCm39)	W524*	probably null	Het
Rhox4d	G	A	X: 36,700,645 (GRCm39)	G191E	unknown	Het
Rims1	A	G	1: 22,474,699 (GRCm39)	S211P		Het
Ripor2	A	T	13: 24,854,054 (GRCm39)	Q54L	possibly damaging	Het
Rnf213	A	G	11: 119,372,117 (GRCm39)	I4903V		Het
Robo3	T	A	9: 37,336,111 (GRCm39)	I452F	possibly damaging	Het
Rpl27rt	A	G	18: 34,870,580 (GRCm39)	K38R	probably damaging	Het
Seh1l	C	T	18: 67,916,988 (GRCm39)	H56Y	probably damaging	Het
Sema3b	T	A	9: 107,480,162 (GRCm39)	D192V	probably damaging	Het
Sidt1	A	T	16: 44,106,763 (GRCm39)	C222*	probably null	Het
Skor1	A	G	9: 63,053,385 (GRCm39)	F195L	probably damaging	Het
Slc44a2	A	C	9: 21,259,642 (GRCm39)	K596N	possibly damaging	Het
Smgc	A	G	15: 91,729,696 (GRCm39)	K217E	probably benign	Het
Socs7	C	A	11: 97,267,917 (GRCm39)	H349Q	possibly damaging	Het
Speer4f2	A	G	5: 17,581,540 (GRCm39)	T161A		Het
Spg11	T	C	2: 121,924,026 (GRCm39)		probably null	Het
Ssb	A	G	2: 69,693,624 (GRCm39)	T11A	probably benign	Het
Sun1	A	G	5: 139,232,589 (GRCm39)	S837G	probably damaging	Het
Szt2	A	C	4: 118,220,668 (GRCm39)	S3385A	unknown	Het
Tapbp	T	C	17: 34,139,391 (GRCm39)	V129A	possibly damaging	Het
Thsd1	T	C	8: 22,733,349 (GRCm39)	I132T	possibly damaging	Het
Tm6sf2	T	A	8: 70,530,589 (GRCm39)	V223E	possibly damaging	Het
Tm9sf4	T	A	2: 153,036,267 (GRCm39)	M343K	probably benign	Het
Trank1	C	A	9: 111,195,417 (GRCm39)	P1147Q	probably benign	Het
Trip4	G	A	9: 65,773,757 (GRCm39)	T275M	probably damaging	Het
Tsen34	T	C	7: 3,698,834 (GRCm39)		probably null	Het
Ttn	T	C	2: 76,680,422 (GRCm39)	E1086G	unknown	Het
Ubr4	A	T	4: 139,189,778 (GRCm39)	M853L	unknown	Het
Ubxn11	A	T	4: 133,852,466 (GRCm39)	R352W	probably damaging	Het
Vmn1r35	G	A	6: 66,656,219 (GRCm39)		probably benign	Het
Vmn2r107	C	T	17: 20,595,994 (GRCm39)	T849I	probably benign	Het
Vmn2r93	C	A	17: 18,546,248 (GRCm39)	L707I	probably benign	Het
Wwc1	G	A	11: 35,766,533 (GRCm39)	T574I	probably benign	Het
Zfp143	A	G	7: 109,669,705 (GRCm39)	M45V	probably benign	Het
Zfp518a	T	A	19: 40,902,601 (GRCm39)	N843K	possibly damaging	Het
Zfp87	A	T	13: 67,665,163 (GRCm39)	M433K	probably benign	Het
Zfp873	C	A	10: 81,896,461 (GRCm39)	H397Q	probably damaging	Het
Zscan21	A	T	5: 138,116,110 (GRCm39)		probably benign	Het

Other mutations in Magi2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00908:Magi2	APN	5	20,596,299 (GRCm39)	missense	probably benign	0.05
IGL02120:Magi2	APN	5	20,433,451 (GRCm39)	critical splice donor site	probably null
IGL02341:Magi2	APN	5	20,671,201 (GRCm39)	missense	probably damaging	1.00
IGL02411:Magi2	APN	5	19,883,707 (GRCm39)	missense	probably damaging	1.00
IGL02657:Magi2	APN	5	19,432,581 (GRCm39)	missense	probably damaging	0.99
IGL02976:Magi2	APN	5	20,739,473 (GRCm39)	missense	probably damaging	1.00
IGL03105:Magi2	APN	5	20,748,616 (GRCm39)	missense	probably damaging	0.97
IGL03246:Magi2	APN	5	20,563,948 (GRCm39)	missense	probably damaging	1.00
IGL03329:Magi2	APN	5	20,671,126 (GRCm39)	missense	possibly damaging	0.95
LCD18:Magi2	UTSW	5	20,159,509 (GRCm39)	intron	probably benign
PIT4519001:Magi2	UTSW	5	20,866,344 (GRCm39)	missense	probably damaging	1.00
R0009:Magi2	UTSW	5	20,816,053 (GRCm39)	missense	probably benign	0.15
R0009:Magi2	UTSW	5	20,816,053 (GRCm39)	missense	probably benign	0.15
R0352:Magi2	UTSW	5	20,270,664 (GRCm39)	missense	probably damaging	1.00
R0362:Magi2	UTSW	5	19,432,573 (GRCm39)	missense	probably damaging	1.00
R0496:Magi2	UTSW	5	20,866,357 (GRCm39)	splice site	probably benign
R1103:Magi2	UTSW	5	20,816,101 (GRCm39)	missense	probably damaging	1.00
R1435:Magi2	UTSW	5	20,563,943 (GRCm39)	missense	probably damaging	1.00
R1583:Magi2	UTSW	5	19,432,330 (GRCm39)	missense	probably benign	0.30
R1616:Magi2	UTSW	5	20,814,324 (GRCm39)	missense	probably damaging	1.00
R1643:Magi2	UTSW	5	20,910,504 (GRCm39)	unclassified	probably benign
R1707:Magi2	UTSW	5	20,420,491 (GRCm39)	missense	probably damaging	1.00
R1833:Magi2	UTSW	5	19,432,455 (GRCm39)	missense	probably damaging	1.00
R1837:Magi2	UTSW	5	20,670,825 (GRCm39)	missense	probably damaging	1.00
R1838:Magi2	UTSW	5	20,670,825 (GRCm39)	missense	probably damaging	1.00
R1839:Magi2	UTSW	5	20,670,825 (GRCm39)	missense	probably damaging	1.00
R1847:Magi2	UTSW	5	20,807,458 (GRCm39)	missense	probably damaging	0.99
R2223:Magi2	UTSW	5	20,670,670 (GRCm39)	missense	probably damaging	1.00
R2496:Magi2	UTSW	5	19,883,750 (GRCm39)	missense	probably benign	0.42
R2504:Magi2	UTSW	5	20,563,934 (GRCm39)	missense	probably damaging	1.00
R2848:Magi2	UTSW	5	20,807,459 (GRCm39)	frame shift	probably null
R2879:Magi2	UTSW	5	20,807,459 (GRCm39)	frame shift	probably null
R2935:Magi2	UTSW	5	20,807,459 (GRCm39)	frame shift	probably null
R2936:Magi2	UTSW	5	20,807,459 (GRCm39)	frame shift	probably null
R3694:Magi2	UTSW	5	20,807,459 (GRCm39)	frame shift	probably null
R3783:Magi2	UTSW	5	20,670,907 (GRCm39)	missense	probably damaging	0.97
R3786:Magi2	UTSW	5	20,670,907 (GRCm39)	missense	probably damaging	0.97
R3787:Magi2	UTSW	5	20,670,907 (GRCm39)	missense	probably damaging	0.97
R3837:Magi2	UTSW	5	20,420,466 (GRCm39)	missense	probably benign	0.28
R4151:Magi2	UTSW	5	19,432,290 (GRCm39)	missense	probably damaging	0.97
R4721:Magi2	UTSW	5	20,739,467 (GRCm39)	missense	probably damaging	1.00
R5005:Magi2	UTSW	5	20,739,444 (GRCm39)	missense	probably damaging	0.98
R5012:Magi2	UTSW	5	20,670,618 (GRCm39)	missense	probably damaging	0.99
R5193:Magi2	UTSW	5	20,563,970 (GRCm39)	critical splice donor site	probably null
R5298:Magi2	UTSW	5	20,774,160 (GRCm39)	missense	probably damaging	1.00
R5372:Magi2	UTSW	5	20,907,108 (GRCm39)	missense	possibly damaging	0.82
R5580:Magi2	UTSW	5	20,420,422 (GRCm39)	missense	probably benign	0.03
R5806:Magi2	UTSW	5	20,856,202 (GRCm39)	missense	probably benign	0.01
R5924:Magi2	UTSW	5	20,816,067 (GRCm39)	missense	probably benign	0.00
R5992:Magi2	UTSW	5	19,432,289 (GRCm39)	start codon destroyed	probably null	0.42
R6014:Magi2	UTSW	5	20,816,091 (GRCm39)	missense	probably damaging	1.00
R6073:Magi2	UTSW	5	20,774,286 (GRCm39)	missense	probably damaging	1.00
R6500:Magi2	UTSW	5	20,807,345 (GRCm39)	missense	possibly damaging	0.94
R6664:Magi2	UTSW	5	20,907,395 (GRCm39)	missense	probably benign	0.00
R7229:Magi2	UTSW	5	20,670,586 (GRCm39)	missense	probably damaging	1.00
R7344:Magi2	UTSW	5	20,755,238 (GRCm39)	missense	probably benign	0.19
R7605:Magi2	UTSW	5	20,433,383 (GRCm39)	missense	probably damaging	1.00
R7712:Magi2	UTSW	5	20,755,280 (GRCm39)	missense	possibly damaging	0.78
R7808:Magi2	UTSW	5	20,670,838 (GRCm39)	missense	probably benign	0.03
R7955:Magi2	UTSW	5	20,594,070 (GRCm39)	missense	probably damaging	1.00
R8134:Magi2	UTSW	5	20,596,392 (GRCm39)	missense	probably benign	0.03
R8134:Magi2	UTSW	5	20,596,365 (GRCm39)	missense	probably damaging	1.00
R8253:Magi2	UTSW	5	20,814,305 (GRCm39)	missense	probably benign	0.44
R8481:Magi2	UTSW	5	20,594,152 (GRCm39)	missense	possibly damaging	0.91
R8553:Magi2	UTSW	5	20,856,198 (GRCm39)	missense	probably benign	0.00
R8751:Magi2	UTSW	5	20,739,462 (GRCm39)	missense	probably benign
R8766:Magi2	UTSW	5	20,400,123 (GRCm39)	missense	probably benign	0.33
R8851:Magi2	UTSW	5	20,270,618 (GRCm39)	missense	probably damaging	1.00
R8876:Magi2	UTSW	5	20,856,190 (GRCm39)	nonsense	probably null
R9120:Magi2	UTSW	5	20,733,305 (GRCm39)	missense	possibly damaging	0.81
R9335:Magi2	UTSW	5	20,866,263 (GRCm39)	missense
R9367:Magi2	UTSW	5	20,766,308 (GRCm39)	missense	probably damaging	0.97
R9454:Magi2	UTSW	5	20,671,176 (GRCm39)	missense	probably damaging	0.97
R9474:Magi2	UTSW	5	20,400,019 (GRCm39)	missense	probably benign	0.00
R9577:Magi2	UTSW	5	20,814,282 (GRCm39)	missense	probably damaging	1.00
R9673:Magi2	UTSW	5	20,670,582 (GRCm39)	missense	possibly damaging	0.86
R9696:Magi2	UTSW	5	20,670,864 (GRCm39)	missense	probably benign	0.35
X0065:Magi2	UTSW	5	20,774,176 (GRCm39)	missense	possibly damaging	0.94
Z1176:Magi2	UTSW	5	20,907,107 (GRCm39)	missense	probably benign	0.32
Z1177:Magi2	UTSW	5	20,907,410 (GRCm39)	missense	probably benign

Predicted Primers

PCR Primer
(F):5'- CACAATGGGTCTGAAATTTAGGC -3'
(R):5'- CATCAGCCAGCTGCAGAATC -3'

Sequencing Primer
(F):5'- TTTAGGCAAATGCTGACGGC -3'
(R):5'- TCAAGAAACACTAGCCATGCTGG -3'

Posted On

2019-10-07