Incidental Mutation 'R0633:Nek10'
| ID |
58100 |
| Institutional Source |
Beutler Lab
|
| Gene Symbol |
Nek10
|
| Ensembl Gene |
ENSMUSG00000042567 |
| Gene Name |
NIMA (never in mitosis gene a)- related kinase 10 |
| Synonyms |
LOC238944 |
| MMRRC Submission |
038822-MU
|
| Accession Numbers |
|
| Essential gene? |
Non essential
(E-score: 0.000)
|
| Stock # |
R0633 (G1)
|
| Quality Score |
176 |
|
Status
|
Not validated
|
| Chromosome |
14 |
| Chromosomal Location |
7457704-7666183 bp(-) (GRCm39) |
| Type of Mutation |
critical splice acceptor site |
| DNA Base Change (assembly) |
G to A
at 14857782 bp (GRCm38)
|
| Zygosity |
Heterozygous |
| Amino Acid Change |
|
| Ref Sequence |
ENSEMBL: ENSMUSP00000153142
(fasta)
|
| Gene Model |
predicted gene model for transcript(s):
[ENSMUST00000112630]
[ENSMUST00000112631]
[ENSMUST00000224491]
|
| AlphaFold |
Q3UGM2 |
| Predicted Effect |
noncoding transcript
Transcript: ENSMUST00000063220
|
| Predicted Effect |
probably null
Transcript: ENSMUST00000112630
|
| SMART Domains |
Protein: ENSMUSP00000108249
Gene: ENSMUSG00000042567
| Domain | Start | End | E-Value | Type |
|---|
|
ARM
|
197 |
238 |
8.23e1 |
SMART |
|
ARM
|
278 |
320 |
5.18e0 |
SMART |
|
low complexity region
|
387 |
400 |
N/A |
INTRINSIC |
|
ARM
|
401 |
448 |
7.09e1 |
SMART |
|
S_TKc
|
519 |
791 |
2.36e-75 |
SMART |
|
low complexity region
|
799 |
811 |
N/A |
INTRINSIC |
|
low complexity region
|
839 |
863 |
N/A |
INTRINSIC |
|
low complexity region
|
908 |
926 |
N/A |
INTRINSIC |
|
| Predicted Effect |
probably null
Transcript: ENSMUST00000112631
|
| SMART Domains |
Protein: ENSMUSP00000108250
Gene: ENSMUSG00000042567
| Domain | Start | End | E-Value | Type |
|---|
|
ARM
|
197 |
238 |
8.23e1 |
SMART |
|
ARM
|
278 |
320 |
5.18e0 |
SMART |
|
low complexity region
|
387 |
400 |
N/A |
INTRINSIC |
|
ARM
|
401 |
448 |
7.09e1 |
SMART |
|
S_TKc
|
519 |
791 |
2.36e-75 |
SMART |
|
low complexity region
|
799 |
811 |
N/A |
INTRINSIC |
|
low complexity region
|
839 |
863 |
N/A |
INTRINSIC |
|
low complexity region
|
908 |
926 |
N/A |
INTRINSIC |
|
| Predicted Effect |
probably null
Transcript: ENSMUST00000224491
|
| Coding Region Coverage |
- 1x: 99.3%
- 3x: 98.8%
- 10x: 97.4%
- 20x: 94.7%
|
| Validation Efficiency |
|
| Allele List at MGI |
|
| Other mutations in this stock |
Total: 77 list
| Gene | Ref | Var | Chr/Loc | Mutation | Predicted Effect | Zygosity |
|---|
| Adamts8 |
A |
T |
9: 30,854,807 (GRCm39) |
R18S |
probably damaging |
Het |
| Adgb |
G |
A |
10: 10,267,473 (GRCm39) |
A923V |
probably benign |
Het |
| Aldh1a3 |
A |
G |
7: 66,049,970 (GRCm39) |
V416A |
probably damaging |
Het |
| Alox5 |
C |
T |
6: 116,397,345 (GRCm39) |
G280R |
probably damaging |
Het |
| Anapc5 |
A |
T |
5: 122,938,695 (GRCm39) |
Y360N |
probably damaging |
Het |
| Apbb1 |
C |
T |
7: 105,208,170 (GRCm39) |
V685I |
probably damaging |
Het |
| Apc2 |
C |
A |
10: 80,143,289 (GRCm39) |
A463E |
probably damaging |
Het |
| Arhgap21 |
C |
T |
2: 20,860,198 (GRCm39) |
W1170* |
probably null |
Het |
| Atat1 |
G |
A |
17: 36,212,315 (GRCm39) |
R305C |
probably damaging |
Het |
| Brd8dc |
A |
G |
18: 34,719,319 (GRCm39) |
V167A |
possibly damaging |
Het |
| Cars2 |
T |
C |
8: 11,600,511 (GRCm39) |
D56G |
probably benign |
Het |
| Ccdc202 |
T |
G |
14: 96,119,379 (GRCm39) |
N45K |
probably damaging |
Het |
| Cdc42bpb |
T |
C |
12: 111,311,989 (GRCm39) |
I108V |
probably damaging |
Het |
| Cftr |
T |
A |
6: 18,305,979 (GRCm39) |
I1255K |
probably damaging |
Het |
| Ckap5 |
T |
C |
2: 91,381,088 (GRCm39) |
L148P |
probably damaging |
Het |
| Cntn4 |
A |
G |
6: 106,656,209 (GRCm39) |
|
probably null |
Het |
| Cpe |
G |
A |
8: 65,062,237 (GRCm39) |
P273L |
probably damaging |
Het |
| Cpsf7 |
A |
G |
19: 10,509,146 (GRCm39) |
D19G |
probably benign |
Het |
| Ddx25 |
C |
A |
9: 35,457,268 (GRCm39) |
R349L |
probably damaging |
Het |
| Depdc7 |
T |
C |
2: 104,553,226 (GRCm39) |
D446G |
probably benign |
Het |
| Det1 |
T |
A |
7: 78,493,683 (GRCm39) |
N107I |
probably benign |
Het |
| Dock6 |
A |
T |
9: 21,755,713 (GRCm39) |
D170E |
probably benign |
Het |
| Dvl1 |
C |
T |
4: 155,942,752 (GRCm39) |
L673F |
probably damaging |
Het |
| Gucy1b1 |
A |
T |
3: 81,952,767 (GRCm39) |
I222K |
probably benign |
Het |
| Hfm1 |
T |
C |
5: 107,065,467 (GRCm39) |
T71A |
possibly damaging |
Het |
| Ikzf1 |
A |
G |
11: 11,719,223 (GRCm39) |
E310G |
probably damaging |
Het |
| Impg1 |
T |
C |
9: 80,301,437 (GRCm39) |
E163G |
possibly damaging |
Het |
| Itpr2 |
G |
T |
6: 146,275,954 (GRCm39) |
H426Q |
probably damaging |
Het |
| Itpripl2 |
C |
T |
7: 118,089,479 (GRCm39) |
G360D |
probably benign |
Het |
| Kif14 |
C |
T |
1: 136,455,043 (GRCm39) |
R1572C |
probably damaging |
Het |
| L3mbtl3 |
A |
T |
10: 26,178,583 (GRCm39) |
H568Q |
unknown |
Het |
| Lgi2 |
A |
G |
5: 52,711,802 (GRCm39) |
Y173H |
probably damaging |
Het |
| Lpar5 |
A |
C |
6: 125,058,954 (GRCm39) |
Y225S |
probably benign |
Het |
| Lpin3 |
A |
G |
2: 160,745,894 (GRCm39) |
H675R |
probably damaging |
Het |
| Lrp2 |
C |
A |
2: 69,278,464 (GRCm39) |
G3963V |
probably damaging |
Het |
| Man1a2 |
G |
T |
3: 100,591,891 (GRCm39) |
D13E |
possibly damaging |
Het |
| Map1a |
T |
C |
2: 121,138,495 (GRCm39) |
V2753A |
probably damaging |
Het |
| Mitf |
C |
A |
6: 97,980,865 (GRCm39) |
N97K |
probably damaging |
Het |
| Msh2 |
A |
G |
17: 87,980,238 (GRCm39) |
|
probably null |
Het |
| Msr1 |
T |
C |
8: 40,073,041 (GRCm39) |
E170G |
probably damaging |
Het |
| Myrip |
C |
A |
9: 120,217,302 (GRCm39) |
R79S |
probably damaging |
Het |
| Neto1 |
C |
T |
18: 86,422,854 (GRCm39) |
R104* |
probably null |
Het |
| Nom1 |
A |
C |
5: 29,656,098 (GRCm39) |
K821T |
probably damaging |
Het |
| Nrxn1 |
A |
G |
17: 91,011,609 (GRCm39) |
V340A |
probably damaging |
Het |
| Nxpe4 |
A |
T |
9: 48,307,897 (GRCm39) |
I334F |
probably benign |
Het |
| Or1e23 |
A |
G |
11: 73,407,753 (GRCm39) |
S91P |
probably benign |
Het |
| Or2ag1 |
T |
C |
7: 106,313,184 (GRCm39) |
K235E |
probably benign |
Het |
| Or4a74 |
T |
C |
2: 89,439,718 (GRCm39) |
M243V |
probably benign |
Het |
| Or5al7 |
T |
A |
2: 85,992,435 (GRCm39) |
N286I |
probably damaging |
Het |
| Or5b124 |
T |
C |
19: 13,610,700 (GRCm39) |
V75A |
probably damaging |
Het |
| Or8k27 |
C |
T |
2: 86,275,473 (GRCm39) |
M284I |
probably benign |
Het |
| Padi4 |
A |
G |
4: 140,484,896 (GRCm39) |
S322P |
probably damaging |
Het |
| Peli3 |
A |
G |
19: 4,991,810 (GRCm39) |
Y44H |
probably damaging |
Het |
| Prdm4 |
A |
G |
10: 85,743,767 (GRCm39) |
S163P |
probably damaging |
Het |
| Prom2 |
T |
C |
2: 127,381,445 (GRCm39) |
D227G |
probably benign |
Het |
| Ptgfr |
C |
T |
3: 151,507,400 (GRCm39) |
R321H |
probably benign |
Het |
| Resf1 |
A |
T |
6: 149,227,199 (GRCm39) |
I82L |
probably benign |
Het |
| Rgs3 |
G |
A |
4: 62,544,143 (GRCm39) |
R136H |
probably damaging |
Het |
| Rgsl1 |
T |
G |
1: 153,719,853 (GRCm39) |
N3T |
possibly damaging |
Het |
| Rif1 |
T |
C |
2: 52,002,575 (GRCm39) |
S2010P |
probably benign |
Het |
| Rngtt |
T |
C |
4: 33,368,690 (GRCm39) |
F408L |
probably damaging |
Het |
| Rtn3 |
T |
G |
19: 7,434,958 (GRCm39) |
T326P |
probably benign |
Het |
| Slc18b1 |
A |
C |
10: 23,681,936 (GRCm39) |
M167L |
probably benign |
Het |
| Slc22a26 |
A |
G |
19: 7,765,575 (GRCm39) |
|
probably null |
Het |
| Slitrk6 |
T |
C |
14: 110,989,317 (GRCm39) |
D130G |
probably damaging |
Het |
| Snap47 |
A |
G |
11: 59,319,439 (GRCm39) |
V233A |
probably benign |
Het |
| Sumf1 |
A |
C |
6: 108,121,632 (GRCm39) |
Y158D |
probably damaging |
Het |
| Tbc1d15 |
A |
T |
10: 115,056,215 (GRCm39) |
H252Q |
probably benign |
Het |
| Thsd7b |
T |
C |
1: 130,116,263 (GRCm39) |
S1339P |
possibly damaging |
Het |
| Tmem45a2 |
T |
C |
16: 56,869,777 (GRCm39) |
I56V |
probably benign |
Het |
| Ttc21b |
A |
G |
2: 66,066,577 (GRCm39) |
S359P |
probably benign |
Het |
| Ttc27 |
T |
C |
17: 75,036,972 (GRCm39) |
I215T |
probably benign |
Het |
| Ttn |
C |
T |
2: 76,554,539 (GRCm39) |
V30759I |
possibly damaging |
Het |
| Vdac3 |
T |
C |
8: 23,070,404 (GRCm39) |
N168S |
probably damaging |
Het |
| Wdr7 |
T |
C |
18: 63,998,371 (GRCm39) |
V1106A |
probably benign |
Het |
| Wrap73 |
T |
A |
4: 154,226,948 (GRCm39) |
F16Y |
probably damaging |
Het |
| Zfat |
C |
A |
15: 68,052,652 (GRCm39) |
D381Y |
probably damaging |
Het |
|
| Other mutations in Nek10 |
| Allele | Source | Chr | Coord | Type | Predicted Effect | PPH Score |
|---|
|
IGL01361:Nek10
|
APN |
14 |
14,850,957 (GRCm38) |
missense |
probably damaging |
0.99 |
|
IGL02067:Nek10
|
APN |
14 |
14,861,639 (GRCm38) |
missense |
probably benign |
0.12 |
|
IGL02361:Nek10
|
APN |
14 |
14,843,856 (GRCm38) |
missense |
probably damaging |
1.00 |
|
IGL02687:Nek10
|
APN |
14 |
14,840,570 (GRCm38) |
missense |
probably damaging |
1.00 |
|
IGL02929:Nek10
|
APN |
14 |
14,821,119 (GRCm38) |
missense |
possibly damaging |
0.82 |
|
IGL03229:Nek10
|
APN |
14 |
14,986,686 (GRCm38) |
missense |
probably benign |
0.10 |
|
P0041:Nek10
|
UTSW |
14 |
14,861,603 (GRCm38) |
missense |
probably benign |
0.01 |
|
R0007:Nek10
|
UTSW |
14 |
14,840,574 (GRCm38) |
missense |
probably benign |
0.10 |
|
R0007:Nek10
|
UTSW |
14 |
14,840,574 (GRCm38) |
missense |
probably benign |
0.10 |
|
R0142:Nek10
|
UTSW |
14 |
14,861,560 (GRCm38) |
missense |
possibly damaging |
0.96 |
|
R0433:Nek10
|
UTSW |
14 |
14,860,927 (GRCm38) |
missense |
probably benign |
0.32 |
|
R1087:Nek10
|
UTSW |
14 |
14,827,059 (GRCm38) |
missense |
possibly damaging |
0.59 |
|
R1184:Nek10
|
UTSW |
14 |
14,931,325 (GRCm38) |
splice site |
probably benign |
|
|
R1250:Nek10
|
UTSW |
14 |
14,853,887 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R1371:Nek10
|
UTSW |
14 |
14,850,983 (GRCm38) |
missense |
probably damaging |
0.98 |
|
R1506:Nek10
|
UTSW |
14 |
14,999,078 (GRCm38) |
splice site |
probably benign |
|
|
R1829:Nek10
|
UTSW |
14 |
14,863,454 (GRCm38) |
critical splice acceptor site |
probably null |
|
|
R1831:Nek10
|
UTSW |
14 |
14,842,789 (GRCm38) |
missense |
probably benign |
|
|
R1833:Nek10
|
UTSW |
14 |
14,842,789 (GRCm38) |
missense |
probably benign |
|
|
R1990:Nek10
|
UTSW |
14 |
14,860,764 (GRCm38) |
missense |
probably benign |
|
|
R1997:Nek10
|
UTSW |
14 |
14,827,003 (GRCm38) |
missense |
probably benign |
0.09 |
|
R2011:Nek10
|
UTSW |
14 |
14,885,122 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R2158:Nek10
|
UTSW |
14 |
14,885,047 (GRCm38) |
splice site |
probably null |
|
|
R2288:Nek10
|
UTSW |
14 |
14,853,956 (GRCm38) |
nonsense |
probably null |
|
|
R2568:Nek10
|
UTSW |
14 |
14,999,112 (GRCm38) |
missense |
possibly damaging |
0.89 |
|
R2907:Nek10
|
UTSW |
14 |
14,980,613 (GRCm38) |
missense |
possibly damaging |
0.81 |
|
R2965:Nek10
|
UTSW |
14 |
14,836,202 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R3922:Nek10
|
UTSW |
14 |
14,861,585 (GRCm38) |
missense |
possibly damaging |
0.88 |
|
R4032:Nek10
|
UTSW |
14 |
14,853,877 (GRCm38) |
splice site |
probably null |
|
|
R4700:Nek10
|
UTSW |
14 |
14,842,841 (GRCm38) |
missense |
possibly damaging |
0.69 |
|
R4742:Nek10
|
UTSW |
14 |
14,861,624 (GRCm38) |
missense |
probably null |
0.03 |
|
R4785:Nek10
|
UTSW |
14 |
14,855,714 (GRCm38) |
missense |
probably benign |
|
|
R4890:Nek10
|
UTSW |
14 |
14,860,986 (GRCm38) |
missense |
possibly damaging |
0.47 |
|
R4891:Nek10
|
UTSW |
14 |
14,860,986 (GRCm38) |
missense |
possibly damaging |
0.47 |
|
R4920:Nek10
|
UTSW |
14 |
14,860,986 (GRCm38) |
missense |
possibly damaging |
0.47 |
|
R4924:Nek10
|
UTSW |
14 |
14,846,594 (GRCm38) |
splice site |
probably null |
|
|
R4928:Nek10
|
UTSW |
14 |
14,930,577 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R4948:Nek10
|
UTSW |
14 |
14,860,986 (GRCm38) |
missense |
possibly damaging |
0.47 |
|
R4952:Nek10
|
UTSW |
14 |
14,860,986 (GRCm38) |
missense |
possibly damaging |
0.47 |
|
R4953:Nek10
|
UTSW |
14 |
14,860,986 (GRCm38) |
missense |
possibly damaging |
0.47 |
|
R5092:Nek10
|
UTSW |
14 |
14,820,851 (GRCm38) |
missense |
possibly damaging |
0.81 |
|
R5097:Nek10
|
UTSW |
14 |
14,857,851 (GRCm38) |
missense |
probably benign |
0.00 |
|
R5593:Nek10
|
UTSW |
14 |
14,980,544 (GRCm38) |
nonsense |
probably null |
|
|
R5696:Nek10
|
UTSW |
14 |
14,860,736 (GRCm38) |
splice site |
probably null |
|
|
R5813:Nek10
|
UTSW |
14 |
14,986,704 (GRCm38) |
missense |
probably benign |
0.01 |
|
R5829:Nek10
|
UTSW |
14 |
14,865,404 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R5872:Nek10
|
UTSW |
14 |
14,850,896 (GRCm38) |
missense |
probably benign |
0.06 |
|
R5939:Nek10
|
UTSW |
14 |
14,931,290 (GRCm38) |
missense |
possibly damaging |
0.58 |
|
R6025:Nek10
|
UTSW |
14 |
14,865,633 (GRCm38) |
missense |
probably benign |
0.41 |
|
R6235:Nek10
|
UTSW |
14 |
14,821,113 (GRCm38) |
nonsense |
probably null |
|
|
R6539:Nek10
|
UTSW |
14 |
14,860,789 (GRCm38) |
missense |
possibly damaging |
0.94 |
|
R6542:Nek10
|
UTSW |
14 |
14,999,108 (GRCm38) |
missense |
probably benign |
0.44 |
|
R6561:Nek10
|
UTSW |
14 |
14,828,448 (GRCm38) |
missense |
possibly damaging |
0.48 |
|
R6659:Nek10
|
UTSW |
14 |
14,861,684 (GRCm38) |
missense |
probably benign |
0.29 |
|
R7039:Nek10
|
UTSW |
14 |
14,986,700 (GRCm38) |
missense |
probably damaging |
0.99 |
|
R7039:Nek10
|
UTSW |
14 |
14,826,946 (GRCm38) |
missense |
possibly damaging |
0.63 |
|
R7102:Nek10
|
UTSW |
14 |
14,828,517 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R7185:Nek10
|
UTSW |
14 |
14,846,621 (GRCm38) |
missense |
probably benign |
0.03 |
|
R7198:Nek10
|
UTSW |
14 |
14,850,947 (GRCm38) |
missense |
probably damaging |
0.99 |
|
R7202:Nek10
|
UTSW |
14 |
14,836,171 (GRCm38) |
missense |
probably benign |
0.01 |
|
R7251:Nek10
|
UTSW |
14 |
14,853,965 (GRCm38) |
missense |
probably benign |
|
|
R7345:Nek10
|
UTSW |
14 |
14,955,503 (GRCm38) |
missense |
probably benign |
|
|
R7590:Nek10
|
UTSW |
14 |
15,006,693 (GRCm38) |
makesense |
probably null |
|
|
R7593:Nek10
|
UTSW |
14 |
14,826,955 (GRCm38) |
missense |
probably benign |
0.04 |
|
R7616:Nek10
|
UTSW |
14 |
14,937,759 (GRCm38) |
missense |
probably benign |
0.27 |
|
R7635:Nek10
|
UTSW |
14 |
14,850,932 (GRCm38) |
missense |
probably benign |
0.01 |
|
R7817:Nek10
|
UTSW |
14 |
15,001,017 (GRCm38) |
missense |
probably benign |
0.00 |
|
R7826:Nek10
|
UTSW |
14 |
14,860,846 (GRCm38) |
splice site |
probably null |
|
|
R7986:Nek10
|
UTSW |
14 |
15,001,020 (GRCm38) |
missense |
probably benign |
0.17 |
|
R8765:Nek10
|
UTSW |
14 |
14,999,104 (GRCm38) |
missense |
probably damaging |
0.97 |
|
R8856:Nek10
|
UTSW |
14 |
14,937,610 (GRCm38) |
missense |
probably damaging |
0.96 |
|
R8973:Nek10
|
UTSW |
14 |
14,931,321 (GRCm38) |
critical splice donor site |
probably null |
|
|
R9002:Nek10
|
UTSW |
14 |
14,980,590 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R9088:Nek10
|
UTSW |
14 |
14,931,314 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R9195:Nek10
|
UTSW |
14 |
14,821,139 (GRCm38) |
missense |
probably benign |
0.03 |
|
R9464:Nek10
|
UTSW |
14 |
14,937,766 (GRCm38) |
missense |
probably benign |
|
|
R9511:Nek10
|
UTSW |
14 |
14,828,511 (GRCm38) |
missense |
probably benign |
0.05 |
|
R9529:Nek10
|
UTSW |
14 |
14,850,833 (GRCm38) |
missense |
probably benign |
|
|
R9590:Nek10
|
UTSW |
14 |
14,853,888 (GRCm38) |
missense |
probably damaging |
1.00 |
|
Z1177:Nek10
|
UTSW |
14 |
15,001,157 (GRCm38) |
nonsense |
probably null |
|
|
Z1177:Nek10
|
UTSW |
14 |
14,853,948 (GRCm38) |
missense |
probably benign |
0.00 |
|
| Predicted Primers |
PCR Primer
(F):5'- ACAGGGTGTTTCATTGGCACAGG -3'
(R):5'- GGTTTTCATAGACTCCATCCCCAAGC -3'
Sequencing Primer
(F):5'- gattacaagcaagccaccttac -3'
(R):5'- GAGCCCACTGCTAACCTG -3'
|
| Posted On |
2013-07-11 |
Incidental Mutation