Mutagenetix > Incidental Mutation

Incidental Mutation 'R7529:Tg'

583227

Institutional Source

Beutler Lab

Gene Symbol

Ensembl Gene

ENSMUSG00000053469

Gene Name

thyroglobulin

Synonyms

Tgn

MMRRC Submission

045601-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.096) question?

Stock #

R7529 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

66542606-66722570 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

G to T at 66566617 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Glycine to Tryptophan at position 1222 (G1222W)

Ref Sequence

ENSEMBL: ENSMUSP00000070239 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000065916]

AlphaFold

no structure available at present

Predicted Effect

probably damaging
Transcript: ENSMUST00000065916
AA Change: G1222W

PolyPhen 2 Score 0.990 (Sensitivity: 0.72; Specificity: 0.97)

SMART Domains

Protein: ENSMUSP00000070239
Gene: ENSMUSG00000053469
AA Change: G1222W

Domain	Start	End	E-Value	Type
signal peptide	1	20	N/A	INTRINSIC
TY	50	97	5.9e-16	SMART
TY	118	165	5.59e-17	SMART
Pfam:Thyroglobulin_1	174	252	4e-9	PFAM
TY	317	363	4.36e-19	SMART
low complexity region	495	504	N/A	INTRINSIC
TY	617	662	3.58e-15	SMART
TY	684	730	1.47e-16	SMART
TY	880	926	1.51e-4	SMART
TY	1029	1078	1.21e-12	SMART
TY	1106	1150	7.56e-5	SMART
TY	1167	1215	7.26e-16	SMART
low complexity region	1244	1255	N/A	INTRINSIC
Pfam:GCC2_GCC3	1464	1509	2.7e-16	PFAM
TY	1519	1568	9.81e-13	SMART
Pfam:COesterase	2181	2717	8.4e-140	PFAM

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.7%
20x: 99.1%

Validation Efficiency

100% (79/79)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] Thyroglobulin (Tg) is a glycoprotein homodimer produced predominantly by the thryroid gland. It acts as a substrate for the synthesis of thyroxine and triiodothyronine as well as the storage of the inactive forms of thyroid hormone and iodine. Thyroglobulin is secreted from the endoplasmic reticulum to its site of iodination, and subsequent thyroxine biosynthesis, in the follicular lumen. Mutations in this gene cause thyroid dyshormonogenesis, manifested as goiter, and are associated with moderate to severe congenital hypothyroidism. Polymorphisms in this gene are associated with susceptibility to autoimmune thyroid diseases (AITD) such as Graves disease and Hashimoto thryoiditis. [provided by RefSeq, Nov 2009]
PHENOTYPE: Mice homozygous for a spontaneous mutation exhibit enlarged thyroid gland, hypothyroidism, abnormal thyroid gland morphology, and decreased body weight. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 79 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
9130023H24Rik	T	A	7: 127,836,336 (GRCm39)	K86*	probably null	Het
Ablim3	A	C	18: 61,955,039 (GRCm39)	S317A	probably benign	Het
Adcy1	T	C	11: 7,089,157 (GRCm39)	S524P	probably damaging	Het
Agps	C	A	2: 75,662,696 (GRCm39)	A47E	possibly damaging	Het
Arhgef15	G	T	11: 68,844,848 (GRCm39)	R250S	probably damaging	Het
Armh1	C	A	4: 117,070,938 (GRCm39)	A396S	probably benign	Het
Atoh8	G	T	6: 72,200,825 (GRCm39)	D288E	probably benign	Het
B3galt2	A	C	1: 143,522,274 (GRCm39)	K137Q	probably benign	Het
Cacna2d4	G	A	6: 119,247,727 (GRCm39)	V343I	probably benign	Het
Cage1	T	C	13: 38,209,731 (GRCm39)	N82S	possibly damaging	Het
Cep350	A	G	1: 155,737,669 (GRCm39)	S2725P	probably benign	Het
Cfap65	T	C	1: 74,965,769 (GRCm39)	N414D	probably damaging	Het
Cgnl1	A	C	9: 71,539,040 (GRCm39)	L1154R	probably damaging	Het
Cmya5	A	T	13: 93,233,942 (GRCm39)	M382K	probably benign	Het
Dchs2	G	A	3: 83,261,705 (GRCm39)	V2658M	possibly damaging	Het
Dlgap5	T	C	14: 47,653,876 (GRCm39)	N51S	probably damaging	Het
Dnah17	A	G	11: 117,940,692 (GRCm39)		probably null	Het
Dnal1	A	G	12: 84,178,117 (GRCm39)	I35V	probably benign	Het
Dtnbp1	A	G	13: 45,084,546 (GRCm39)	F198S	probably damaging	Het
Edar	A	T	10: 58,447,830 (GRCm39)	S160T	probably benign	Het
Enpp3	A	T	10: 24,674,072 (GRCm39)	N409K	probably damaging	Het
Ercc6	T	A	14: 32,282,686 (GRCm39)	C726*	probably null	Het
Extl2	G	A	3: 115,821,055 (GRCm39)	V301I	possibly damaging	Het
G2e3	A	G	12: 51,418,387 (GRCm39)	Q594R	probably damaging	Het
Gal3st2c	A	G	1: 93,937,039 (GRCm39)	N328S	probably benign	Het
Galnt17	A	G	5: 131,335,218 (GRCm39)	V74A	probably damaging	Het
Ggta1	A	G	2: 35,304,256 (GRCm39)	W76R	probably damaging	Het
Gm9772	T	A	17: 22,226,140 (GRCm39)	D48V	probably benign	Het
Herpud2	T	C	9: 25,020,193 (GRCm39)	T388A	probably damaging	Het
Ighv5-9-1	A	G	12: 113,699,954 (GRCm39)	S53P	possibly damaging	Het
Il23r	T	A	6: 67,467,720 (GRCm39)	M16L	possibly damaging	Het
Ints1	G	A	5: 139,753,481 (GRCm39)	A717V	possibly damaging	Het
Itpr2	A	T	6: 146,096,096 (GRCm39)	L2122Q	probably damaging	Het
Klrg2	A	T	6: 38,607,266 (GRCm39)	V248E	probably damaging	Het
Krtap5-5	A	G	7: 141,783,429 (GRCm39)	C74R	unknown	Het
Luzp1	G	T	4: 136,268,243 (GRCm39)	L155F	probably damaging	Het
Mcam	T	C	9: 44,050,192 (GRCm39)	V209A	probably benign	Het
Med1	G	C	11: 98,046,791 (GRCm39)	T1335R	unknown	Het
Mroh2b	A	G	15: 4,978,491 (GRCm39)	I1346V	probably damaging	Het
Mrpl4	C	G	9: 20,918,975 (GRCm39)	Q201E	probably benign	Het
Muc21	A	T	17: 35,930,123 (GRCm39)	S1354R	unknown	Het
Mylk2	T	C	2: 152,757,624 (GRCm39)	L326P	probably damaging	Het
Myot	A	T	18: 44,479,240 (GRCm39)	R326*	probably null	Het
Nox3	T	A	17: 3,722,050 (GRCm39)	R288S	probably damaging	Het
Nox4	A	T	7: 87,044,976 (GRCm39)	Y572F	unknown	Het
Or2v2	A	G	11: 49,003,686 (GRCm39)	L289P	probably damaging	Het
Or5an10	A	G	19: 12,276,086 (GRCm39)	S137P	probably damaging	Het
Pcdhb15	G	T	18: 37,607,526 (GRCm39)	E253*	probably null	Het
Plcb2	T	C	2: 118,540,715 (GRCm39)	H1052R	probably damaging	Het
Plpp5	A	T	8: 26,214,233 (GRCm39)	Q250L	probably benign	Het
Plxna2	T	C	1: 194,326,179 (GRCm39)	Y38H	probably benign	Het
Pnpla8	A	G	12: 44,329,963 (GRCm39)	K172E	probably benign	Het
Pramel23	T	C	4: 143,429,244 (GRCm39)			Het
Prl8a9	T	A	13: 27,744,511 (GRCm39)	D110V	probably benign	Het
Prrx1	T	C	1: 163,081,533 (GRCm39)		probably null	Het
Prss52	T	A	14: 64,347,037 (GRCm39)	H70Q	probably benign	Het
Rcc1	T	C	4: 132,061,874 (GRCm39)	T300A	probably benign	Het
Rnf114	T	C	2: 167,349,014 (GRCm39)	V64A	possibly damaging	Het
Rnf168	T	C	16: 32,117,732 (GRCm39)	I431T	probably damaging	Het
Rnmt	A	G	18: 68,444,726 (GRCm39)	M232V	probably benign	Het
Rrs1	A	C	1: 9,616,417 (GRCm39)	Q223H	probably benign	Het
Scyl3	A	T	1: 163,771,438 (GRCm39)	L261F	probably damaging	Het
Slc24a4	A	T	12: 102,230,707 (GRCm39)	T533S	probably benign	Het
Slc26a2	A	G	18: 61,331,430 (GRCm39)	L667P	probably damaging	Het
Snrnp40	T	A	4: 130,278,275 (GRCm39)	V260D	possibly damaging	Het
Snrpa	A	C	7: 26,888,878 (GRCm39)	M174R	probably benign	Het
Ss18l1	C	T	2: 179,699,950 (GRCm39)	A270V	possibly damaging	Het
Stk19	G	T	17: 35,043,632 (GRCm39)	Q193K	probably benign	Het
Syne1	T	A	10: 5,374,382 (GRCm39)	I142L	probably damaging	Het
Tbx2	T	C	11: 85,731,727 (GRCm39)	S675P	probably benign	Het
Tcaf1	A	G	6: 42,652,289 (GRCm39)	I731T	probably damaging	Het
Tsc2	A	G	17: 24,816,922 (GRCm39)	F1581L	probably damaging	Het
Ubr4	G	C	4: 139,149,728 (GRCm39)	V520L	probably benign	Het
Wls	A	T	3: 159,578,644 (GRCm39)	N69Y	probably benign	Het
Wnk2	A	G	13: 49,254,457 (GRCm39)	F353L	possibly damaging	Het
Xkr6	G	T	14: 64,056,610 (GRCm39)	V430F	probably benign	Het
Zfp64	C	T	2: 168,735,992 (GRCm39)	G562R	probably benign	Het
Zfp663	T	G	2: 165,194,728 (GRCm39)	E497A	probably damaging	Het
Zfp995	A	T	17: 22,099,333 (GRCm39)	C300*	probably null	Het

Other mutations in Tg

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00157:Tg	APN	15	66,719,015 (GRCm39)	missense	probably damaging	1.00
IGL00230:Tg	APN	15	66,699,139 (GRCm39)	missense	probably benign	0.00
IGL00324:Tg	APN	15	66,565,273 (GRCm39)	missense	probably benign
IGL00428:Tg	APN	15	66,645,273 (GRCm39)	missense	probably benign	0.33
IGL00703:Tg	APN	15	66,568,338 (GRCm39)	missense	probably benign	0.34
IGL00808:Tg	APN	15	66,555,662 (GRCm39)	missense	probably damaging	1.00
IGL00833:Tg	APN	15	66,560,650 (GRCm39)	missense	probably benign	0.34
IGL00899:Tg	APN	15	66,545,922 (GRCm39)	critical splice donor site	probably null
IGL00921:Tg	APN	15	66,636,302 (GRCm39)	missense	probably benign	0.28
IGL00975:Tg	APN	15	66,553,731 (GRCm39)	missense	probably benign
IGL01288:Tg	APN	15	66,608,125 (GRCm39)	missense	possibly damaging	0.81
IGL01397:Tg	APN	15	66,567,941 (GRCm39)	splice site	probably benign
IGL01634:Tg	APN	15	66,601,415 (GRCm39)	missense	probably benign	0.34
IGL01646:Tg	APN	15	66,549,936 (GRCm39)	missense	probably damaging	1.00
IGL01704:Tg	APN	15	66,543,200 (GRCm39)	missense	probably damaging	0.98
IGL01958:Tg	APN	15	66,631,335 (GRCm39)	missense	probably benign	0.06
IGL02093:Tg	APN	15	66,564,223 (GRCm39)	missense	possibly damaging	0.83
IGL02113:Tg	APN	15	66,577,179 (GRCm39)	missense	probably benign	0.08
IGL02138:Tg	APN	15	66,589,082 (GRCm39)	missense	probably benign	0.01
IGL02156:Tg	APN	15	66,577,197 (GRCm39)	missense	probably benign	0.19
IGL02169:Tg	APN	15	66,629,792 (GRCm39)	missense	probably benign	0.04
IGL02342:Tg	APN	15	66,636,140 (GRCm39)	missense	probably benign
IGL02434:Tg	APN	15	66,636,191 (GRCm39)	missense	probably damaging	0.97
IGL02506:Tg	APN	15	66,613,443 (GRCm39)	missense	possibly damaging	0.71
IGL02513:Tg	APN	15	66,577,123 (GRCm39)	missense	probably benign
IGL02549:Tg	APN	15	66,711,210 (GRCm39)	missense	probably damaging	1.00
IGL02669:Tg	APN	15	66,620,575 (GRCm39)	splice site	probably benign
IGL02756:Tg	APN	15	66,606,435 (GRCm39)	missense	probably benign
IGL02800:Tg	APN	15	66,629,735 (GRCm39)	missense	probably damaging	1.00
IGL02828:Tg	APN	15	66,554,243 (GRCm39)	missense	probably damaging	1.00
IGL02927:Tg	APN	15	66,549,942 (GRCm39)	missense	probably damaging	1.00
IGL03061:Tg	APN	15	66,543,254 (GRCm39)	missense	probably damaging	1.00
IGL03105:Tg	APN	15	66,586,955 (GRCm39)	missense	probably benign	0.01
IGL03160:Tg	APN	15	66,711,152 (GRCm39)	nonsense	probably null
IGL03242:Tg	APN	15	66,555,647 (GRCm39)	missense	probably damaging	0.99
Also_ran	UTSW	15	66,550,688 (GRCm39)	missense	probably damaging	1.00
bedraggled	UTSW	15	66,612,563 (GRCm39)	missense	probably damaging	1.00
foster	UTSW	15	66,565,109 (GRCm39)	nonsense	probably null
hognose	UTSW	15	66,589,057 (GRCm39)	missense	probably damaging	0.99
ito	UTSW	15	66,638,011 (GRCm39)	nonsense	probably null
ito2	UTSW	15	66,543,245 (GRCm39)	missense	probably damaging	1.00
ito3	UTSW	15	66,645,323 (GRCm39)	missense	probably damaging	1.00
ito4	UTSW	15	66,568,369 (GRCm39)	missense	possibly damaging	0.47
Papua	UTSW	15	66,545,899 (GRCm39)	missense	probably damaging	1.00
Pipistrella	UTSW	15	66,567,984 (GRCm39)	missense	probably damaging	1.00
pluribus	UTSW	15	66,587,012 (GRCm39)	missense	probably damaging	0.98
samarai	UTSW	15	66,629,855 (GRCm39)	critical splice donor site	probably null
sariba	UTSW	15	66,566,719 (GRCm39)	missense	probably benign	0.01
ticker	UTSW	15	66,699,231 (GRCm39)	nonsense	probably null
Vampire	UTSW	15	66,554,676 (GRCm39)	missense	probably damaging	1.00
IGL03134:Tg	UTSW	15	66,612,567 (GRCm39)	missense	probably damaging	1.00
P0019:Tg	UTSW	15	66,560,712 (GRCm39)	missense	probably benign	0.01
R0121:Tg	UTSW	15	66,612,630 (GRCm39)	missense	probably benign	0.04
R0135:Tg	UTSW	15	66,566,719 (GRCm39)	missense	probably benign	0.01
R0227:Tg	UTSW	15	66,570,295 (GRCm39)	missense	possibly damaging	0.84
R0448:Tg	UTSW	15	66,636,291 (GRCm39)	missense	probably damaging	1.00
R0453:Tg	UTSW	15	66,700,382 (GRCm39)	missense	probably benign	0.09
R0504:Tg	UTSW	15	66,554,253 (GRCm39)	missense	probably damaging	0.97
R0543:Tg	UTSW	15	66,601,446 (GRCm39)	missense	probably benign	0.13
R0638:Tg	UTSW	15	66,589,057 (GRCm39)	missense	probably damaging	0.99
R0639:Tg	UTSW	15	66,613,333 (GRCm39)	critical splice acceptor site	probably null
R0646:Tg	UTSW	15	66,601,475 (GRCm39)	missense	probably damaging	0.99
R0666:Tg	UTSW	15	66,609,370 (GRCm39)	missense	probably benign
R0673:Tg	UTSW	15	66,613,333 (GRCm39)	critical splice acceptor site	probably null
R0689:Tg	UTSW	15	66,711,253 (GRCm39)	splice site	probably benign
R0704:Tg	UTSW	15	66,629,729 (GRCm39)	missense	probably benign	0.02
R0730:Tg	UTSW	15	66,550,638 (GRCm39)	missense	probably damaging	1.00
R0830:Tg	UTSW	15	66,596,993 (GRCm39)	missense	probably damaging	1.00
R0959:Tg	UTSW	15	66,579,859 (GRCm39)	missense	probably damaging	0.98
R1027:Tg	UTSW	15	66,544,258 (GRCm39)	missense	possibly damaging	0.65
R1061:Tg	UTSW	15	66,570,408 (GRCm39)	missense	probably benign	0.09
R1086:Tg	UTSW	15	66,555,911 (GRCm39)	missense	probably benign
R1103:Tg	UTSW	15	66,591,504 (GRCm39)	missense	probably benign	0.45
R1240:Tg	UTSW	15	66,700,397 (GRCm39)	missense	probably benign	0.16
R1281:Tg	UTSW	15	66,568,338 (GRCm39)	missense	probably benign	0.34
R1470:Tg	UTSW	15	66,721,312 (GRCm39)	missense	possibly damaging	0.95
R1470:Tg	UTSW	15	66,721,312 (GRCm39)	missense	possibly damaging	0.95
R1531:Tg	UTSW	15	66,722,351 (GRCm39)	missense	probably benign	0.02
R1544:Tg	UTSW	15	66,577,081 (GRCm39)	missense	probably benign	0.04
R1550:Tg	UTSW	15	66,565,279 (GRCm39)	missense	possibly damaging	0.52
R1575:Tg	UTSW	15	66,601,534 (GRCm39)	critical splice donor site	probably null
R1638:Tg	UTSW	15	66,568,015 (GRCm39)	nonsense	probably null
R1655:Tg	UTSW	15	66,700,417 (GRCm39)	critical splice donor site	probably null
R1671:Tg	UTSW	15	66,564,236 (GRCm39)	missense	possibly damaging	0.89
R1789:Tg	UTSW	15	66,609,397 (GRCm39)	missense	probably benign	0.00
R1883:Tg	UTSW	15	66,543,158 (GRCm39)	missense	probably damaging	1.00
R1984:Tg	UTSW	15	66,554,691 (GRCm39)	missense	probably benign
R2063:Tg	UTSW	15	66,700,402 (GRCm39)	missense	probably damaging	1.00
R2092:Tg	UTSW	15	66,721,456 (GRCm39)	missense	probably null	0.26
R2109:Tg	UTSW	15	66,601,443 (GRCm39)	missense	probably benign	0.02
R2128:Tg	UTSW	15	66,566,743 (GRCm39)	missense	probably benign	0.10
R2129:Tg	UTSW	15	66,566,743 (GRCm39)	missense	probably benign	0.10
R2207:Tg	UTSW	15	66,553,788 (GRCm39)	missense	probably benign	0.15
R2219:Tg	UTSW	15	66,553,782 (GRCm39)	missense	probably benign	0.03
R2228:Tg	UTSW	15	66,545,860 (GRCm39)	missense	probably damaging	0.99
R2229:Tg	UTSW	15	66,545,860 (GRCm39)	missense	probably damaging	0.99
R2259:Tg	UTSW	15	66,555,747 (GRCm39)	missense	probably benign
R2994:Tg	UTSW	15	66,553,802 (GRCm39)	missense	probably benign
R3904:Tg	UTSW	15	66,638,011 (GRCm39)	nonsense	probably null
R3946:Tg	UTSW	15	66,545,872 (GRCm39)	missense	probably damaging	1.00
R3965:Tg	UTSW	15	66,556,039 (GRCm39)	missense	probably benign
R4245:Tg	UTSW	15	66,568,318 (GRCm39)	missense	possibly damaging	0.68
R4451:Tg	UTSW	15	66,637,996 (GRCm39)	missense	probably benign	0.01
R4487:Tg	UTSW	15	66,543,245 (GRCm39)	missense	probably damaging	1.00
R4489:Tg	UTSW	15	66,579,791 (GRCm39)	missense	probably damaging	1.00
R4623:Tg	UTSW	15	66,607,120 (GRCm39)	missense	probably benign	0.23
R4659:Tg	UTSW	15	66,545,769 (GRCm39)	missense	possibly damaging	0.67
R4728:Tg	UTSW	15	66,554,676 (GRCm39)	missense	probably damaging	1.00
R4760:Tg	UTSW	15	66,565,168 (GRCm39)	missense	probably damaging	1.00
R4797:Tg	UTSW	15	66,629,855 (GRCm39)	critical splice donor site	probably null
R4944:Tg	UTSW	15	66,636,186 (GRCm39)	missense	probably damaging	1.00
R4998:Tg	UTSW	15	66,545,899 (GRCm39)	missense	probably damaging	1.00
R5009:Tg	UTSW	15	66,568,435 (GRCm39)	missense	probably benign	0.01
R5025:Tg	UTSW	15	66,579,779 (GRCm39)	missense	probably damaging	1.00
R5035:Tg	UTSW	15	66,553,662 (GRCm39)	splice site	probably null
R5049:Tg	UTSW	15	66,699,231 (GRCm39)	nonsense	probably null
R5073:Tg	UTSW	15	66,607,101 (GRCm39)	missense	probably benign	0.05
R5169:Tg	UTSW	15	66,550,629 (GRCm39)	nonsense	probably null
R5185:Tg	UTSW	15	66,645,323 (GRCm39)	missense	probably damaging	1.00
R5227:Tg	UTSW	15	66,631,416 (GRCm39)	missense	possibly damaging	0.87
R5300:Tg	UTSW	15	66,550,704 (GRCm39)	missense	probably damaging	1.00
R5334:Tg	UTSW	15	66,549,904 (GRCm39)	missense	probably damaging	1.00
R5339:Tg	UTSW	15	66,549,942 (GRCm39)	missense	probably damaging	1.00
R5402:Tg	UTSW	15	66,611,017 (GRCm39)	missense	probably damaging	0.98
R5441:Tg	UTSW	15	66,568,369 (GRCm39)	missense	possibly damaging	0.47
R5509:Tg	UTSW	15	66,699,142 (GRCm39)	missense	probably benign	0.45
R5580:Tg	UTSW	15	66,557,149 (GRCm39)	missense	possibly damaging	0.66
R5582:Tg	UTSW	15	66,565,284 (GRCm39)	missense	probably damaging	1.00
R5624:Tg	UTSW	15	66,709,906 (GRCm39)	missense	probably benign	0.11
R5686:Tg	UTSW	15	66,560,738 (GRCm39)	missense	probably benign	0.28
R6042:Tg	UTSW	15	66,555,842 (GRCm39)	missense	probably benign	0.01
R6122:Tg	UTSW	15	66,700,306 (GRCm39)	missense	probably damaging	1.00
R6146:Tg	UTSW	15	66,545,216 (GRCm39)	splice site	probably null
R6159:Tg	UTSW	15	66,607,096 (GRCm39)	missense	possibly damaging	0.71
R6223:Tg	UTSW	15	66,579,771 (GRCm39)	missense	probably benign	0.15
R6480:Tg	UTSW	15	66,543,160 (GRCm39)	missense	probably damaging	1.00
R6505:Tg	UTSW	15	66,631,407 (GRCm39)	missense	probably damaging	0.99
R6531:Tg	UTSW	15	66,711,211 (GRCm39)	missense	probably damaging	0.99
R6614:Tg	UTSW	15	66,607,108 (GRCm39)	missense	probably damaging	0.99
R6698:Tg	UTSW	15	66,711,211 (GRCm39)	missense	probably damaging	1.00
R6798:Tg	UTSW	15	66,550,688 (GRCm39)	missense	probably damaging	1.00
R6837:Tg	UTSW	15	66,567,984 (GRCm39)	missense	probably damaging	1.00
R6861:Tg	UTSW	15	66,560,740 (GRCm39)	missense	probably benign	0.00
R6888:Tg	UTSW	15	66,568,095 (GRCm39)	missense	probably damaging	0.99
R6933:Tg	UTSW	15	66,636,158 (GRCm39)	missense	possibly damaging	0.73
R6983:Tg	UTSW	15	66,565,207 (GRCm39)	missense	probably benign	0.01
R7078:Tg	UTSW	15	66,545,392 (GRCm39)	missense	probably damaging	1.00
R7244:Tg	UTSW	15	66,612,563 (GRCm39)	missense	probably damaging	1.00
R7320:Tg	UTSW	15	66,566,633 (GRCm39)	missense	possibly damaging	0.71
R7334:Tg	UTSW	15	66,597,121 (GRCm39)	missense	probably benign	0.01
R7418:Tg	UTSW	15	66,568,432 (GRCm39)	missense	probably damaging	0.99
R7485:Tg	UTSW	15	66,568,437 (GRCm39)	missense	probably benign	0.04
R7524:Tg	UTSW	15	66,568,010 (GRCm39)	missense	probably benign	0.01
R7540:Tg	UTSW	15	66,561,776 (GRCm39)	missense	probably benign	0.16
R7583:Tg	UTSW	15	66,636,267 (GRCm39)	missense	probably damaging	1.00
R7594:Tg	UTSW	15	66,601,432 (GRCm39)	missense	probably benign	0.20
R7667:Tg	UTSW	15	66,587,012 (GRCm39)	missense	probably damaging	0.98
R7722:Tg	UTSW	15	66,636,158 (GRCm39)	missense	possibly damaging	0.73
R7790:Tg	UTSW	15	66,721,453 (GRCm39)	missense	probably damaging	0.99
R7838:Tg	UTSW	15	66,565,112 (GRCm39)	missense	probably benign	0.00
R7890:Tg	UTSW	15	66,555,663 (GRCm39)	missense	probably damaging	1.00
R7904:Tg	UTSW	15	66,577,128 (GRCm39)	missense	probably benign	0.08
R7919:Tg	UTSW	15	66,555,923 (GRCm39)	missense	possibly damaging	0.73
R7921:Tg	UTSW	15	66,555,642 (GRCm39)	missense	probably benign	0.08
R8037:Tg	UTSW	15	66,560,724 (GRCm39)	missense	probably benign	0.00
R8038:Tg	UTSW	15	66,560,724 (GRCm39)	missense	probably benign	0.00
R8214:Tg	UTSW	15	66,645,247 (GRCm39)	missense	probably damaging	1.00
R8304:Tg	UTSW	15	66,565,109 (GRCm39)	nonsense	probably null
R8688:Tg	UTSW	15	66,566,802 (GRCm39)	critical splice donor site	probably benign
R8709:Tg	UTSW	15	66,553,786 (GRCm39)	missense	probably benign	0.08
R8714:Tg	UTSW	15	66,555,891 (GRCm39)	missense	probably damaging	0.97
R8901:Tg	UTSW	15	66,557,184 (GRCm39)	missense	probably damaging	1.00
R8917:Tg	UTSW	15	66,645,332 (GRCm39)	critical splice donor site	probably null
R9023:Tg	UTSW	15	66,555,522 (GRCm39)	missense	probably damaging	1.00
R9232:Tg	UTSW	15	66,570,310 (GRCm39)	missense	probably benign	0.01
R9310:Tg	UTSW	15	66,699,118 (GRCm39)	missense	possibly damaging	0.69
R9361:Tg	UTSW	15	66,557,246 (GRCm39)	missense	possibly damaging	0.50
R9389:Tg	UTSW	15	66,561,173 (GRCm39)	missense	probably benign	0.04
R9501:Tg	UTSW	15	66,718,923 (GRCm39)	missense	possibly damaging	0.52
R9510:Tg	UTSW	15	66,545,913 (GRCm39)	missense	probably damaging	1.00
R9594:Tg	UTSW	15	66,607,109 (GRCm39)	nonsense	probably null
R9629:Tg	UTSW	15	66,555,587 (GRCm39)	missense	possibly damaging	0.95
R9701:Tg	UTSW	15	66,637,991 (GRCm39)	missense	probably benign	0.03
R9743:Tg	UTSW	15	66,561,839 (GRCm39)	missense	probably benign	0.18
R9748:Tg	UTSW	15	66,719,008 (GRCm39)	missense	possibly damaging	0.91
T0975:Tg	UTSW	15	66,560,712 (GRCm39)	missense	probably benign	0.01
X0005:Tg	UTSW	15	66,560,712 (GRCm39)	missense	probably benign	0.01
X0065:Tg	UTSW	15	66,554,303 (GRCm39)	missense	probably damaging	1.00
X0067:Tg	UTSW	15	66,620,592 (GRCm39)	missense	probably benign	0.10
Z1177:Tg	UTSW	15	66,721,396 (GRCm39)	missense	probably benign	0.02
Z1177:Tg	UTSW	15	66,557,159 (GRCm39)	missense	possibly damaging	0.49

Predicted Primers

PCR Primer
(F):5'- GCCTGTCTGGTGAAACTGAG -3'
(R):5'- TACTCACTTTGACAGGCTCG -3'

Sequencing Primer
(F):5'- CTGAGAGACAGTGGACTTATCC -3'
(R):5'- ACTTTGACAGGCTCGGGGTG -3'

Posted On

2019-10-17