Mutagenetix > Incidental Mutation

Incidental Mutation 'R0616:Usp4'

58337

Institutional Source

Beutler Lab

Gene Symbol

Usp4

Ensembl Gene

ENSMUSG00000032612

Gene Name

ubiquitin specific peptidase 4 (proto-oncogene)

Synonyms

Unp, F730026I20Rik

MMRRC Submission

038805-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.139) question?

Stock #

R0616 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

108225052-108269744 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to G at 108244003 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Serine to Alanine at position 247 (S247A)

Ref Sequence

ENSEMBL: ENSMUSP00000141368 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000035237] [ENSMUST00000194224] [ENSMUST00000194959]

AlphaFold

P35123

Predicted Effect

probably benign
Transcript: ENSMUST00000035237
AA Change: S294A

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000035237
Gene: ENSMUSG00000032612
AA Change: S294A

Domain	Start	End	E-Value	Type
DUSP	27	125	1.39e-46	SMART
Pfam:Ubiquitin_3	139	226	6.7e-34	PFAM
low complexity region	263	286	N/A	INTRINSIC
Pfam:UCH	301	919	2.2e-84	PFAM
Pfam:UCH_1	302	507	2.8e-8	PFAM
Pfam:UCH_1	605	901	1.4e-15	PFAM
low complexity region	927	938	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000194034

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000194065

Predicted Effect

probably benign
Transcript: ENSMUST00000194224
AA Change: S294A

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000141321
Gene: ENSMUSG00000032612
AA Change: S294A

Domain	Start	End	E-Value	Type
DUSP	27	125	5.5e-49	SMART
Pfam:Ubiquitin_3	139	226	1.3e-30	PFAM
low complexity region	263	286	N/A	INTRINSIC
Pfam:UCH	301	633	1.4e-50	PFAM
Pfam:UCH_1	302	520	2.3e-8	PFAM
low complexity region	657	673	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000194959
AA Change: S247A

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000141368
Gene: ENSMUSG00000032612
AA Change: S247A

Domain	Start	End	E-Value	Type
DUSP	27	125	5.5e-49	SMART
Pfam:Ubiquitin_3	139	226	1.7e-30	PFAM
Pfam:UCH	254	872	7e-89	PFAM
Pfam:UCH_1	255	469	3.5e-8	PFAM
Pfam:UCH_1	566	854	2.5e-14	PFAM
low complexity region	880	891	N/A	INTRINSIC

Coding Region Coverage

1x: 99.4%
3x: 98.9%
10x: 97.5%
20x: 95.1%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene is a protease that deubiquitinates target proteins such as ADORA2A and TRIM21. The encoded protein shuttles between the nucleus and cytoplasm and is involved in maintaining operational fidelity in the endoplasmic reticulum. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]
PHENOTYPE: Mice homozygous for a knock-out allele exhibit increased irradiation-induced apoptosis, slow cellular growth, resistance to oncogenic transformation, and early cellular replicative senescence. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 89 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca12	G	T	1: 71,341,830 (GRCm39)	Q1044K	probably damaging	Het
Abi3bp	A	T	16: 56,474,433 (GRCm39)	T723S	probably damaging	Het
Ackr3	G	A	1: 90,142,191 (GRCm39)	V217I	probably benign	Het
Acnat2	A	G	4: 49,380,269 (GRCm39)	S370P	probably damaging	Het
Arap1	A	G	7: 101,050,857 (GRCm39)	R1152G	possibly damaging	Het
Arhgap15	G	A	2: 44,006,729 (GRCm39)		probably null	Het
Arhgap5	C	T	12: 52,563,848 (GRCm39)	T273I	possibly damaging	Het
Armh4	G	T	14: 50,011,113 (GRCm39)	T198K	possibly damaging	Het
C1s2	T	C	6: 124,605,723 (GRCm39)	E332G	probably damaging	Het
Camp	G	A	9: 109,677,707 (GRCm39)	R88W	probably benign	Het
Cdkl2	A	G	5: 92,156,863 (GRCm39)	M564T	probably benign	Het
Ceacam20	A	G	7: 19,704,321 (GRCm39)	H124R	probably benign	Het
Cep19	C	T	16: 31,922,829 (GRCm39)	R32C	probably damaging	Het
Cep295	G	A	9: 15,243,618 (GRCm39)	Q1565*	probably null	Het
Chd3	T	C	11: 69,236,313 (GRCm39)	E1932G	probably damaging	Het
Cibar1	G	A	4: 12,168,234 (GRCm39)	R210*	probably null	Het
Cnr2	G	T	4: 135,644,873 (GRCm39)	W317L	probably benign	Het
Cntnap5a	C	T	1: 116,508,279 (GRCm39)	H1264Y	possibly damaging	Het
Depdc7	T	A	2: 104,557,650 (GRCm39)	N200I	probably benign	Het
Dock4	T	C	12: 40,754,414 (GRCm39)	S468P	probably benign	Het
Dscc1	C	A	15: 54,946,966 (GRCm39)	C253F	probably benign	Het
Fam217a	C	A	13: 35,097,666 (GRCm39)	S55I	probably benign	Het
Farp1	G	A	14: 121,514,434 (GRCm39)	R921H	probably damaging	Het
Fat4	A	G	3: 38,997,019 (GRCm39)	D1746G	probably damaging	Het
Fbxw5	T	C	2: 25,392,517 (GRCm39)	F100L	probably damaging	Het
Gli3	C	A	13: 15,836,991 (GRCm39)	T458K	possibly damaging	Het
Gm4841	T	C	18: 60,404,009 (GRCm39)	Y28C	probably benign	Het
Gprc5d	T	C	6: 135,093,430 (GRCm39)	E159G	probably benign	Het
Grm4	A	T	17: 27,653,538 (GRCm39)	I757N	probably damaging	Het
Hagh	A	G	17: 25,076,551 (GRCm39)	Y94C	probably damaging	Het
Hycc1	T	C	5: 24,191,770 (GRCm39)	T44A	probably damaging	Het
Itpkb	T	C	1: 180,249,301 (GRCm39)	I892T	probably damaging	Het
Kcmf1	T	C	6: 72,827,467 (GRCm39)	I58V	probably benign	Het
Khdrbs2	A	G	1: 32,506,856 (GRCm39)	I167V	possibly damaging	Het
Kmt2c	A	G	5: 25,504,250 (GRCm39)	I275T	probably benign	Het
Lingo4	A	G	3: 94,310,388 (GRCm39)	K442R	probably benign	Het
Mak	T	C	13: 41,195,661 (GRCm39)	N382D	probably benign	Het
Maob	G	A	X: 16,576,402 (GRCm39)	T480I	possibly damaging	Het
Mcoln1	A	G	8: 3,565,025 (GRCm39)	E573G	probably benign	Het
Ms4a6b	G	A	19: 11,504,262 (GRCm39)		probably null	Het
Muc5ac	A	G	7: 141,349,981 (GRCm39)	M576V	probably benign	Het
Nme8	T	A	13: 19,875,029 (GRCm39)	D126V	probably benign	Het
Npy2r	T	A	3: 82,448,670 (GRCm39)	D35V	possibly damaging	Het
Nrxn1	T	C	17: 90,670,285 (GRCm39)	D193G	probably damaging	Het
Or2g7	A	C	17: 38,378,131 (GRCm39)	E23A	probably damaging	Het
Or2y1e	T	C	11: 49,218,583 (GRCm39)	L115P	probably damaging	Het
Or4c10b	T	A	2: 89,711,935 (GRCm39)	V255E	probably benign	Het
Or4d5	A	T	9: 40,012,283 (GRCm39)	F168I	probably damaging	Het
Or52b4i	T	A	7: 102,191,761 (GRCm39)	M206K	possibly damaging	Het
Or6d14	A	G	6: 116,533,889 (GRCm39)	I168V	probably benign	Het
Or8b3b	A	T	9: 38,584,776 (GRCm39)	M1K	probably null	Het
Or8c17	G	T	9: 38,180,630 (GRCm39)	V266L	probably benign	Het
Or8g18	G	A	9: 39,148,946 (GRCm39)	T258M	probably benign	Het
Pabpc2	A	T	18: 39,906,792 (GRCm39)	H19L	possibly damaging	Het
Pcdhb9	A	T	18: 37,535,028 (GRCm39)	K341*	probably null	Het
Pde4dip	T	C	3: 97,654,849 (GRCm39)	I859M	probably benign	Het
Pfkfb2	T	C	1: 130,634,159 (GRCm39)		probably null	Het
Pigg	C	T	5: 108,461,951 (GRCm39)	T94M	probably damaging	Het
Pik3c2b	T	C	1: 133,028,569 (GRCm39)	F1353L	probably damaging	Het
Prg4	T	C	1: 150,336,462 (GRCm39)	D87G	probably damaging	Het
Prkdc	A	T	16: 15,508,271 (GRCm39)	D974V	probably damaging	Het
Prmt3	A	G	7: 49,437,076 (GRCm39)	Y217C	probably damaging	Het
Proser1	A	G	3: 53,382,118 (GRCm39)	T192A	probably damaging	Het
Rab3d	G	A	9: 21,826,060 (GRCm39)	T118M	probably damaging	Het
Rb1cc1	A	G	1: 6,314,486 (GRCm39)	K386R	possibly damaging	Het
Rcn2	A	G	9: 55,963,534 (GRCm39)	D221G	probably benign	Het
Rhbdl3	T	G	11: 80,222,687 (GRCm39)	H245Q	probably damaging	Het
Ribc1	T	C	X: 150,788,787 (GRCm39)	E204G	probably damaging	Het
Rpap1	G	A	2: 119,608,601 (GRCm39)	L254F	probably damaging	Het
Rrp12	A	G	19: 41,880,988 (GRCm39)	F148L	possibly damaging	Het
Rusf1	A	T	7: 127,871,803 (GRCm39)		probably null	Het
Setdb1	A	T	3: 95,249,109 (GRCm39)	I333K	probably damaging	Het
Simc1	A	G	13: 54,694,845 (GRCm39)	I1210V	probably benign	Het
Smchd1	T	A	17: 71,686,569 (GRCm39)	D1379V	probably benign	Het
Snap29	A	T	16: 17,240,370 (GRCm39)	K159*	probably null	Het
Spdye4c	A	T	2: 128,436,132 (GRCm39)	K176M	possibly damaging	Het
Stk31	T	A	6: 49,400,419 (GRCm39)	W415R	probably damaging	Het
Supt6	C	T	11: 78,100,321 (GRCm39)	R1497Q	probably damaging	Het
Tenm3	A	G	8: 48,729,191 (GRCm39)	I1605T	possibly damaging	Het
Ttn	T	C	2: 76,676,967 (GRCm39)		probably null	Het
Ttn	A	G	2: 76,728,011 (GRCm39)		probably benign	Het
Ucp3	A	T	7: 100,129,368 (GRCm39)	T68S	probably benign	Het
Ugt2b36	T	C	5: 87,237,336 (GRCm39)	N316D	probably benign	Het
Utp20	A	T	10: 88,606,613 (GRCm39)	V1653D	probably benign	Het
Vmn1r183	A	G	7: 23,754,250 (GRCm39)	I18V	probably benign	Het
Vmn1r237	A	G	17: 21,534,885 (GRCm39)	M203V	probably damaging	Het
Vmn1r61	A	T	7: 5,613,998 (GRCm39)	F105L	possibly damaging	Het
Zfp462	T	C	4: 55,011,951 (GRCm39)	C158R	probably damaging	Het
Zfyve16	C	G	13: 92,657,637 (GRCm39)	R758P	probably damaging	Het

Other mutations in Usp4

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01530:Usp4	APN	9	108,240,099 (GRCm39)	critical splice donor site	probably null
IGL01663:Usp4	APN	9	108,243,079 (GRCm39)	missense	possibly damaging	0.80
IGL02105:Usp4	APN	9	108,262,131 (GRCm39)	missense	probably damaging	1.00
IGL02486:Usp4	APN	9	108,228,228 (GRCm39)	missense	probably damaging	1.00
kleinesrot	UTSW	9	108,233,661 (GRCm39)	missense	possibly damaging	0.95
R0148:Usp4	UTSW	9	108,268,870 (GRCm39)	splice site	probably null
R0285:Usp4	UTSW	9	108,255,763 (GRCm39)	missense	probably benign	0.33
R0591:Usp4	UTSW	9	108,225,228 (GRCm39)	splice site	probably benign
R0594:Usp4	UTSW	9	108,248,080 (GRCm39)	splice site	probably null
R1329:Usp4	UTSW	9	108,249,765 (GRCm39)	missense	probably damaging	1.00
R1508:Usp4	UTSW	9	108,249,873 (GRCm39)	missense	probably benign	0.14
R1752:Usp4	UTSW	9	108,251,441 (GRCm39)	missense	probably damaging	1.00
R1824:Usp4	UTSW	9	108,225,207 (GRCm39)	missense	probably damaging	1.00
R1846:Usp4	UTSW	9	108,249,935 (GRCm39)	missense	probably benign
R2196:Usp4	UTSW	9	108,250,885 (GRCm39)	missense	probably benign	0.07
R2925:Usp4	UTSW	9	108,245,055 (GRCm39)	missense	probably damaging	1.00
R4126:Usp4	UTSW	9	108,237,316 (GRCm39)	missense	probably benign	0.10
R4345:Usp4	UTSW	9	108,245,222 (GRCm39)	intron	probably benign
R4965:Usp4	UTSW	9	108,239,819 (GRCm39)	missense	probably damaging	1.00
R4981:Usp4	UTSW	9	108,258,617 (GRCm39)	missense	probably benign	0.00
R5110:Usp4	UTSW	9	108,239,877 (GRCm39)	missense	probably damaging	1.00
R5580:Usp4	UTSW	9	108,243,058 (GRCm39)	missense	probably benign	0.09
R5586:Usp4	UTSW	9	108,233,661 (GRCm39)	missense	possibly damaging	0.95
R5927:Usp4	UTSW	9	108,268,959 (GRCm39)	missense	probably benign	0.09
R6025:Usp4	UTSW	9	108,237,322 (GRCm39)	missense	possibly damaging	0.70
R6112:Usp4	UTSW	9	108,233,703 (GRCm39)	missense	probably damaging	1.00
R6197:Usp4	UTSW	9	108,248,154 (GRCm39)	missense	probably damaging	1.00
R6742:Usp4	UTSW	9	108,251,438 (GRCm39)	missense	possibly damaging	0.74
R7320:Usp4	UTSW	9	108,265,505 (GRCm39)	missense	probably benign	0.00
R7458:Usp4	UTSW	9	108,245,055 (GRCm39)	missense	probably damaging	1.00
R7531:Usp4	UTSW	9	108,249,879 (GRCm39)	missense	probably damaging	1.00
R7563:Usp4	UTSW	9	108,256,543 (GRCm39)	missense	probably benign
R8022:Usp4	UTSW	9	108,255,670 (GRCm39)	missense	probably damaging	0.99
R8510:Usp4	UTSW	9	108,265,581 (GRCm39)	critical splice donor site	probably null
R8996:Usp4	UTSW	9	108,268,909 (GRCm39)	missense	probably damaging	1.00
R9151:Usp4	UTSW	9	108,244,011 (GRCm39)	missense	probably benign	0.00
R9775:Usp4	UTSW	9	108,239,780 (GRCm39)	missense	probably damaging	1.00
X0026:Usp4	UTSW	9	108,225,069 (GRCm39)	unclassified	probably benign

Predicted Primers

PCR Primer
(F):5'- GATGAACACTCAGGCCCAGTCAAG -3'
(R):5'- AGCAGCCAGTAGGAGATATCCTAGC -3'

Sequencing Primer
(F):5'- GTGGCACATGTAATGTAAGCTC -3'
(R):5'- GATATCCTAGCAGAGACTTCTGAAGC -3'

Posted On

2013-07-11