Incidental Mutation 'R7546:Trcg1'
| ID |
584220 |
| Institutional Source |
Beutler Lab
|
| Gene Symbol |
Trcg1
|
| Ensembl Gene |
ENSMUSG00000070298 |
| Gene Name |
taste receptor cell gene 1 |
| Synonyms |
|
| MMRRC Submission |
045617-MU
|
| Accession Numbers |
|
| Essential gene? |
Probably non essential
(E-score: 0.070)
|
| Stock # |
R7546 (G1)
|
| Quality Score |
225.009 |
|
Status
|
Validated
|
| Chromosome |
9 |
| Chromosomal Location |
57143839-57157147 bp(+) (GRCm39) |
| Type of Mutation |
missense |
| DNA Base Change (assembly) |
T to C
at 57155621 bp (GRCm39)
|
| Zygosity |
Heterozygous |
| Amino Acid Change |
Leucine to Proline
at position 758
(L758P)
|
| Ref Sequence |
ENSEMBL: ENSMUSP00000091357
(fasta)
|
| Gene Model |
predicted gene model for transcript(s):
[ENSMUST00000034846]
[ENSMUST00000093837]
|
| AlphaFold |
no structure available at present |
| Predicted Effect |
probably benign
Transcript: ENSMUST00000034846
|
| SMART Domains |
Protein: ENSMUSP00000034846
Gene: ENSMUSG00000032300
| Domain | Start | End | E-Value | Type |
|---|
|
low complexity region
|
265 |
284 |
N/A |
INTRINSIC |
|
low complexity region
|
293 |
299 |
N/A |
INTRINSIC |
|
low complexity region
|
334 |
347 |
N/A |
INTRINSIC |
|
low complexity region
|
649 |
675 |
N/A |
INTRINSIC |
|
low complexity region
|
692 |
728 |
N/A |
INTRINSIC |
|
low complexity region
|
785 |
799 |
N/A |
INTRINSIC |
|
low complexity region
|
933 |
951 |
N/A |
INTRINSIC |
|
low complexity region
|
963 |
977 |
N/A |
INTRINSIC |
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000093837
AA Change: L758P
PolyPhen 2
Score 0.337 (Sensitivity: 0.90; Specificity: 0.89)
|
| SMART Domains |
Protein: ENSMUSP00000091357
Gene: ENSMUSG00000070298
AA Change: L758P
| Domain | Start | End | E-Value | Type |
|---|
|
signal peptide
|
1 |
21 |
N/A |
INTRINSIC |
|
low complexity region
|
302 |
335 |
N/A |
INTRINSIC |
|
Blast:SEA
|
449 |
549 |
5e-21 |
BLAST |
|
low complexity region
|
580 |
594 |
N/A |
INTRINSIC |
|
| Meta Mutation Damage Score |
0.0846 |
| Coding Region Coverage |
- 1x: 100.0%
- 3x: 99.9%
- 10x: 99.6%
- 20x: 98.8%
|
| Validation Efficiency |
100% (40/40) |
| Allele List at MGI |
|
| Other mutations in this stock |
Total: 42 list
| Gene | Ref | Var | Chr/Loc | Mutation | Predicted Effect | Zygosity |
|---|
| 1700122O11Rik |
A |
G |
17: 48,348,330 (GRCm39) |
S36P |
probably benign |
Het |
| A530084C06Rik |
G |
T |
13: 31,742,978 (GRCm39) |
R92S |
unknown |
Het |
| Ampd1 |
A |
G |
3: 103,003,028 (GRCm39) |
T582A |
probably benign |
Het |
| Ank1 |
A |
C |
8: 23,555,011 (GRCm39) |
N57T |
probably damaging |
Het |
| Atxn3 |
A |
G |
12: 101,914,261 (GRCm39) |
|
probably null |
Het |
| Bace2 |
G |
A |
16: 97,200,882 (GRCm39) |
A117T |
probably benign |
Het |
| Bcl2l15 |
A |
G |
3: 103,740,203 (GRCm39) |
N19S |
probably benign |
Het |
| Bid |
C |
A |
6: 120,877,112 (GRCm39) |
|
probably null |
Het |
| Cadps2 |
A |
T |
6: 23,626,607 (GRCm39) |
M227K |
probably benign |
Het |
| Clip4 |
G |
A |
17: 72,135,697 (GRCm39) |
C483Y |
possibly damaging |
Het |
| Dennd4a |
A |
G |
9: 64,780,326 (GRCm39) |
T621A |
probably damaging |
Het |
| E2f3 |
G |
A |
13: 30,094,112 (GRCm39) |
S383L |
probably damaging |
Het |
| F2r |
C |
T |
13: 95,754,858 (GRCm39) |
V9I |
probably benign |
Het |
| Gm14226 |
T |
C |
2: 154,867,131 (GRCm39) |
S363P |
probably damaging |
Het |
| Gstm5 |
T |
C |
3: 107,804,610 (GRCm39) |
Y65H |
probably damaging |
Het |
| H2bc13 |
A |
G |
13: 21,900,040 (GRCm39) |
S92P |
probably benign |
Het |
| H60c |
A |
G |
10: 3,209,907 (GRCm39) |
W127R |
probably damaging |
Het |
| Itgav |
G |
T |
2: 83,606,894 (GRCm39) |
G448* |
probably null |
Het |
| Klrh1 |
T |
C |
6: 129,749,343 (GRCm39) |
H84R |
probably benign |
Het |
| Lhx6 |
C |
T |
2: 35,993,357 (GRCm39) |
|
probably null |
Het |
| Manba |
T |
A |
3: 135,276,007 (GRCm39) |
V816D |
probably benign |
Het |
| Marchf10 |
G |
A |
11: 105,280,906 (GRCm39) |
P460S |
not run |
Het |
| Mmp17 |
T |
A |
5: 129,673,653 (GRCm39) |
V244E |
probably damaging |
Het |
| Mtrr |
C |
T |
13: 68,730,268 (GRCm39) |
|
probably benign |
Het |
| Nup160 |
T |
C |
2: 90,515,402 (GRCm39) |
I170T |
probably damaging |
Het |
| Or4c12b |
A |
G |
2: 89,647,363 (GRCm39) |
N225S |
probably benign |
Het |
| Or4c12b |
A |
T |
2: 89,647,538 (GRCm39) |
L283F |
probably damaging |
Het |
| Or51e1 |
T |
C |
7: 102,358,996 (GRCm39) |
S177P |
probably damaging |
Het |
| Pappa |
G |
A |
4: 65,074,352 (GRCm39) |
S302N |
possibly damaging |
Het |
| Plekhj1 |
G |
A |
10: 80,633,748 (GRCm39) |
A53V |
possibly damaging |
Het |
| Pramel46 |
T |
A |
5: 95,418,171 (GRCm39) |
H275L |
possibly damaging |
Het |
| Pramel48 |
C |
T |
5: 95,630,539 (GRCm39) |
R139C |
probably benign |
Het |
| Prpf8 |
T |
C |
11: 75,399,200 (GRCm39) |
V2157A |
probably damaging |
Het |
| Rps8 |
G |
A |
4: 117,011,104 (GRCm39) |
R200W |
probably damaging |
Het |
| Sesn2 |
A |
G |
4: 132,227,154 (GRCm39) |
F93L |
probably damaging |
Het |
| Skic3 |
C |
A |
13: 76,282,954 (GRCm39) |
L759M |
probably damaging |
Het |
| Slf1 |
A |
G |
13: 77,197,311 (GRCm39) |
S768P |
probably benign |
Het |
| Stat4 |
A |
G |
1: 52,137,622 (GRCm39) |
N471S |
probably damaging |
Het |
| Trim35 |
A |
G |
14: 66,540,696 (GRCm39) |
T183A |
probably benign |
Het |
| Ttr |
C |
A |
18: 20,803,102 (GRCm39) |
Y89* |
probably null |
Het |
| Zfp90 |
C |
A |
8: 107,151,323 (GRCm39) |
H345Q |
probably benign |
Het |
| Zmym4 |
A |
G |
4: 126,757,961 (GRCm39) |
V1531A |
probably damaging |
Het |
|
| Other mutations in Trcg1 |
| Allele | Source | Chr | Coord | Type | Predicted Effect | PPH Score |
|---|
|
IGL01727:Trcg1
|
APN |
9 |
57,149,877 (GRCm39) |
missense |
probably damaging |
0.97 |
|
IGL01727:Trcg1
|
APN |
9 |
57,149,556 (GRCm39) |
missense |
probably benign |
|
|
IGL02147:Trcg1
|
APN |
9 |
57,153,132 (GRCm39) |
missense |
probably benign |
0.20 |
|
IGL02329:Trcg1
|
APN |
9 |
57,147,676 (GRCm39) |
missense |
possibly damaging |
0.92 |
|
IGL02658:Trcg1
|
APN |
9 |
57,149,511 (GRCm39) |
nonsense |
probably null |
|
|
IGL02852:Trcg1
|
APN |
9 |
57,148,595 (GRCm39) |
missense |
possibly damaging |
0.94 |
|
IGL03163:Trcg1
|
APN |
9 |
57,155,630 (GRCm39) |
missense |
possibly damaging |
0.92 |
|
FR4589:Trcg1
|
UTSW |
9 |
57,149,485 (GRCm39) |
frame shift |
probably null |
|
|
R0555:Trcg1
|
UTSW |
9 |
57,149,616 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R0747:Trcg1
|
UTSW |
9 |
57,149,204 (GRCm39) |
missense |
probably benign |
0.00 |
|
R1061:Trcg1
|
UTSW |
9 |
57,153,156 (GRCm39) |
missense |
possibly damaging |
0.66 |
|
R1521:Trcg1
|
UTSW |
9 |
57,149,748 (GRCm39) |
missense |
probably benign |
0.36 |
|
R1622:Trcg1
|
UTSW |
9 |
57,155,955 (GRCm39) |
missense |
possibly damaging |
0.94 |
|
R1652:Trcg1
|
UTSW |
9 |
57,152,856 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R4677:Trcg1
|
UTSW |
9 |
57,153,144 (GRCm39) |
missense |
possibly damaging |
0.94 |
|
R4879:Trcg1
|
UTSW |
9 |
57,154,003 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R5013:Trcg1
|
UTSW |
9 |
57,149,562 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R5141:Trcg1
|
UTSW |
9 |
57,148,587 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R5690:Trcg1
|
UTSW |
9 |
57,149,094 (GRCm39) |
missense |
probably benign |
0.36 |
|
R6416:Trcg1
|
UTSW |
9 |
57,148,613 (GRCm39) |
missense |
possibly damaging |
0.46 |
|
R6980:Trcg1
|
UTSW |
9 |
57,152,856 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R7022:Trcg1
|
UTSW |
9 |
57,148,852 (GRCm39) |
missense |
possibly damaging |
0.46 |
|
R7172:Trcg1
|
UTSW |
9 |
57,155,618 (GRCm39) |
missense |
probably benign |
0.01 |
|
R7276:Trcg1
|
UTSW |
9 |
57,149,862 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R7412:Trcg1
|
UTSW |
9 |
57,148,766 (GRCm39) |
missense |
probably benign |
0.00 |
|
R7942:Trcg1
|
UTSW |
9 |
57,149,499 (GRCm39) |
missense |
probably benign |
|
|
R8087:Trcg1
|
UTSW |
9 |
57,155,957 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R8094:Trcg1
|
UTSW |
9 |
57,149,564 (GRCm39) |
missense |
probably benign |
0.01 |
|
R8825:Trcg1
|
UTSW |
9 |
57,148,754 (GRCm39) |
missense |
probably benign |
0.19 |
|
| Predicted Primers |
PCR Primer
(F):5'- AAGAACTTTGGTCAAGTGGTGG -3'
(R):5'- ACTTGGAGCCAATGTACCCTG -3'
Sequencing Primer
(F):5'- AATTCCAGTGAGTGTTCGGTTCAC -3'
(R):5'- CCTGGGGGCTCATAGGGAAG -3'
|
| Posted On |
2019-10-17 |
Incidental Mutation