Mutagenetix > Incidental Mutation

Incidental Mutation 'R0624:Med12l'

58779

Institutional Source

Beutler Lab

Gene Symbol

Med12l

Ensembl Gene

ENSMUSG00000056476

Gene Name

mediator complex subunit 12-like

Synonyms

MMRRC Submission

038813-MU

Accession Numbers

Essential gene?

Possibly non essential (E-score: 0.257) question?

Stock #

R0624 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

58913246-59226103 bp(+) (GRCm39)

Type of Mutation

nonsense

DNA Base Change (assembly)

G to A at 58945123 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Tryptophan to Stop codon at position 116 (W116*)

Ref Sequence

ENSEMBL: ENSMUSP00000142903 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000029393] [ENSMUST00000040325] [ENSMUST00000040846] [ENSMUST00000164225] [ENSMUST00000199659]

AlphaFold

Q8BQM9

Predicted Effect

probably null
Transcript: ENSMUST00000029393
AA Change: W116*

SMART Domains

Protein: ENSMUSP00000029393
Gene: ENSMUSG00000056476
AA Change: W116*

Domain	Start	End	E-Value	Type
Med12	101	172	1.54e-17	SMART
low complexity region	227	235	N/A	INTRINSIC
low complexity region	280	289	N/A	INTRINSIC
Pfam:Med12-LCEWAV	293	737	1.6e-200	PFAM

Predicted Effect

probably null
Transcript: ENSMUST00000040325
AA Change: W116*

SMART Domains

Protein: ENSMUSP00000042269
Gene: ENSMUSG00000056476
AA Change: W116*

Domain	Start	End	E-Value	Type
Med12	101	161	1.71e-24	SMART
low complexity region	216	224	N/A	INTRINSIC
low complexity region	269	278	N/A	INTRINSIC
Pfam:Med12-LCEWAV	282	730	2.6e-207	PFAM
low complexity region	744	758	N/A	INTRINSIC
low complexity region	853	872	N/A	INTRINSIC
low complexity region	1455	1466	N/A	INTRINSIC
low complexity region	1728	1742	N/A	INTRINSIC
low complexity region	1769	1783	N/A	INTRINSIC
Pfam:Med12-PQL	1803	2029	2.3e-14	PFAM
low complexity region	2055	2076	N/A	INTRINSIC
low complexity region	2083	2101	N/A	INTRINSIC
low complexity region	2116	2136	N/A	INTRINSIC

Predicted Effect

probably null
Transcript: ENSMUST00000040846
AA Change: W116*

SMART Domains

Protein: ENSMUSP00000041859
Gene: ENSMUSG00000056476
AA Change: W116*

Domain	Start	End	E-Value	Type
Med12	101	172	1.54e-17	SMART
low complexity region	227	235	N/A	INTRINSIC
low complexity region	280	289	N/A	INTRINSIC
Pfam:Med12-LCEWAV	293	728	9e-201	PFAM

Predicted Effect

probably null
Transcript: ENSMUST00000164225
AA Change: W116*

SMART Domains

Protein: ENSMUSP00000127038
Gene: ENSMUSG00000056476
AA Change: W116*

Domain	Start	End	E-Value	Type
Med12	101	161	1.71e-24	SMART
low complexity region	216	224	N/A	INTRINSIC
low complexity region	269	278	N/A	INTRINSIC
Pfam:Med12-LCEWAV	283	765	5e-187	PFAM
low complexity region	779	793	N/A	INTRINSIC
low complexity region	888	907	N/A	INTRINSIC
low complexity region	1490	1501	N/A	INTRINSIC
low complexity region	1763	1777	N/A	INTRINSIC
low complexity region	1804	1818	N/A	INTRINSIC
Pfam:Med12-PQL	1840	2063	9.7e-66	PFAM
low complexity region	2090	2111	N/A	INTRINSIC
low complexity region	2118	2136	N/A	INTRINSIC
low complexity region	2151	2171	N/A	INTRINSIC

Predicted Effect

probably null
Transcript: ENSMUST00000199659
AA Change: W116*

SMART Domains

Protein: ENSMUSP00000142903
Gene: ENSMUSG00000056476
AA Change: W116*

Domain	Start	End	E-Value	Type
Med12	101	161	1.71e-24	SMART
low complexity region	216	224	N/A	INTRINSIC
low complexity region	269	278	N/A	INTRINSIC
Pfam:Med12-LCEWAV	282	765	5.5e-209	PFAM
low complexity region	779	793	N/A	INTRINSIC
low complexity region	888	907	N/A	INTRINSIC
low complexity region	1490	1501	N/A	INTRINSIC
low complexity region	1761	1775	N/A	INTRINSIC
low complexity region	1802	1816	N/A	INTRINSIC
Pfam:Med12-PQL	1836	2062	1.7e-15	PFAM
low complexity region	2088	2130	N/A	INTRINSIC
low complexity region	2144	2164	N/A	INTRINSIC

Meta Mutation Damage Score

0.9755

Coding Region Coverage

1x: 99.4%
3x: 99.0%
10x: 97.8%
20x: 96.2%

Validation Efficiency

98% (88/90)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene is part of the Mediator complex, which is involved in transcriptional coactivation of nearly all RNA polymerase II-dependent genes. The Mediator complex links gene-specific transcriptional activators with the basal transcription machinery. [provided by RefSeq, May 2010]

Allele List at MGI

All alleles(4) : Targeted(3) Gene trapped(1)

Other mutations in this stock

Total: 88 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca9	T	A	11: 110,030,446 (GRCm39)	D767V	probably damaging	Het
Add1	T	A	5: 34,763,197 (GRCm39)	N128K	probably damaging	Het
Ado	A	T	10: 67,384,058 (GRCm39)	D182E	probably benign	Het
Anapc4	T	A	5: 53,002,761 (GRCm39)		probably benign	Het
Ano10	A	G	9: 122,088,661 (GRCm39)		probably benign	Het
Apba2	A	G	7: 64,364,263 (GRCm39)		probably null	Het
Apc2	A	G	10: 80,150,417 (GRCm39)	T1795A	probably benign	Het
Atp4a	A	G	7: 30,418,424 (GRCm39)	N571D	probably benign	Het
Birc6	A	G	17: 74,887,344 (GRCm39)	N891D	probably benign	Het
Bltp2	G	A	11: 78,159,283 (GRCm39)	E494K	probably damaging	Het
Car3	G	T	3: 14,931,864 (GRCm39)	M78I	probably benign	Het
Cc2d2a	A	T	5: 43,887,371 (GRCm39)	H1267L	probably benign	Het
Cdk18	A	G	1: 132,046,610 (GRCm39)	L192P	probably damaging	Het
Cdk9	A	T	2: 32,599,836 (GRCm39)	Y134N	probably damaging	Het
Ceacam5	A	T	7: 17,448,888 (GRCm39)	T85S	probably benign	Het
Cenpe	A	G	3: 134,952,347 (GRCm39)	T1403A	probably benign	Het
Chd8	C	T	14: 52,457,214 (GRCm39)	G918D	possibly damaging	Het
Csnk1e	T	A	15: 79,304,098 (GRCm39)		probably benign	Het
Dctpp1	A	T	7: 126,856,365 (GRCm39)	I119N	probably damaging	Het
Defb34	T	A	8: 19,173,784 (GRCm39)	F6Y	unknown	Het
Dnai4	T	C	4: 102,930,054 (GRCm39)		probably benign	Het
Dvl1	C	G	4: 155,939,232 (GRCm39)	N248K	probably damaging	Het
Dync1h1	T	C	12: 110,618,181 (GRCm39)		probably benign	Het
Dync2i1	T	C	12: 116,211,910 (GRCm39)	D199G	probably damaging	Het
Eml5	T	A	12: 98,831,738 (GRCm39)	R407W	probably damaging	Het
Epb41l5	T	C	1: 119,551,688 (GRCm39)	D99G	probably damaging	Het
Fat1	A	T	8: 45,504,205 (GRCm39)	N4566I	possibly damaging	Het
Gm21834	T	C	17: 58,049,015 (GRCm39)	E67G	possibly damaging	Het
Gsap	T	A	5: 21,458,949 (GRCm39)		probably null	Het
Guf1	T	C	5: 69,715,923 (GRCm39)	I108T	probably damaging	Het
Hsd3b5	T	C	3: 98,526,720 (GRCm39)	D242G	probably damaging	Het
Kcna7	A	G	7: 45,059,114 (GRCm39)	D467G	probably null	Het
Lars1	A	G	18: 42,375,849 (GRCm39)		probably benign	Het
Lrrc56	A	T	7: 140,786,366 (GRCm39)	D248V	probably damaging	Het
Map3k14	T	A	11: 103,133,117 (GRCm39)	E27V	possibly damaging	Het
Mgll	A	G	6: 88,702,799 (GRCm39)	R33G	probably damaging	Het
Mmp13	A	G	9: 7,280,221 (GRCm39)	S384G	possibly damaging	Het
Nalcn	C	T	14: 123,607,444 (GRCm39)	C675Y	probably benign	Het
Nrxn1	A	G	17: 91,396,117 (GRCm39)	L13P	unknown	Het
Ocstamp	A	G	2: 165,239,772 (GRCm39)	V138A	probably damaging	Het
Or12e8	T	G	2: 87,188,026 (GRCm39)	Y79*	probably null	Het
Or2z8	T	A	8: 72,812,006 (GRCm39)	S161T	possibly damaging	Het
Or4a68	A	G	2: 89,270,482 (GRCm39)	V47A	possibly damaging	Het
Or4f7	T	C	2: 111,645,056 (GRCm39)	N5S	probably damaging	Het
Or5p51	A	G	7: 107,444,323 (GRCm39)	S206P	possibly damaging	Het
Or9i1b	T	G	19: 13,896,808 (GRCm39)	C141W	probably damaging	Het
Patj	T	C	4: 98,569,472 (GRCm39)		probably benign	Het
Pcdhb22	A	G	18: 37,651,780 (GRCm39)	I83V	probably benign	Het
Pclo	A	G	5: 14,719,670 (GRCm39)	E1269G	unknown	Het
Plagl2	T	C	2: 153,077,973 (GRCm39)	T3A	probably benign	Het
Plcb1	C	T	2: 135,136,831 (GRCm39)	P309S	possibly damaging	Het
Pld3	A	T	7: 27,239,000 (GRCm39)	L175Q	possibly damaging	Het
Prrx1	A	G	1: 163,075,974 (GRCm39)		probably benign	Het
Psap	T	G	10: 60,135,345 (GRCm39)		probably benign	Het
Ptgfr	G	A	3: 151,540,839 (GRCm39)	T223M	probably damaging	Het
Reep2	A	T	18: 34,973,824 (GRCm39)	I6F	probably benign	Het
Rraga	A	G	4: 86,494,454 (GRCm39)	E100G	probably benign	Het
Rrm2b	T	C	15: 37,931,889 (GRCm39)	D37G	probably benign	Het
Rtl1	T	C	12: 109,559,153 (GRCm39)	I895M	probably damaging	Het
Ryr1	G	A	7: 28,774,034 (GRCm39)	A2445V	probably damaging	Het
Sbf1	C	T	15: 89,186,532 (GRCm39)	D898N	possibly damaging	Het
Sh3d19	G	A	3: 86,022,213 (GRCm39)	V548I	possibly damaging	Het
Shf	C	A	2: 122,199,116 (GRCm39)		probably benign	Het
Sipa1l3	T	C	7: 29,086,676 (GRCm39)	E638G	probably damaging	Het
Slc13a3	G	T	2: 165,253,807 (GRCm39)	P449T	probably damaging	Het
Slc2a13	C	T	15: 91,234,215 (GRCm39)	V374I	possibly damaging	Het
Slc4a7	T	C	14: 14,794,059 (GRCm38)		probably null	Het
Slc7a2	T	A	8: 41,361,568 (GRCm39)	S414T	probably benign	Het
Slc9c1	A	G	16: 45,393,719 (GRCm39)	E554G	probably benign	Het
Smad2	T	C	18: 76,433,064 (GRCm39)	I332T	probably damaging	Het
Snrnp40	C	T	4: 130,256,451 (GRCm39)	P59S	probably damaging	Het
Sorcs2	A	T	5: 36,222,777 (GRCm39)	I154N	probably damaging	Het
Sort1	G	A	3: 108,255,946 (GRCm39)	G631S	probably damaging	Het
Sox10	T	C	15: 79,043,586 (GRCm39)	D149G	possibly damaging	Het
Spn	C	T	7: 126,735,380 (GRCm39)	V376M	possibly damaging	Het
Tacc2	A	G	7: 130,179,239 (GRCm39)	D9G	probably damaging	Het
Tapt1	T	G	5: 44,334,448 (GRCm39)	L514F	possibly damaging	Het
Tcf3	A	G	10: 80,249,168 (GRCm39)	L480P	probably damaging	Het
Tenm4	G	C	7: 96,423,227 (GRCm39)	G637A	probably damaging	Het
Tex14	T	A	11: 87,411,525 (GRCm39)	N950K	probably benign	Het
Tgfbrap1	T	G	1: 43,098,289 (GRCm39)	H497P	probably benign	Het
Tnfrsf18	A	T	4: 156,110,986 (GRCm39)	Y48F	possibly damaging	Het
Tnxb	A	C	17: 34,902,522 (GRCm39)	H1002P	probably damaging	Het
Ttn	A	G	2: 76,593,571 (GRCm39)		probably benign	Het
Ugt2b34	C	G	5: 87,041,591 (GRCm39)		probably null	Het
Vldlr	A	G	19: 27,215,663 (GRCm39)	D220G	possibly damaging	Het
Vmn1r33	A	T	6: 66,589,121 (GRCm39)	Y144*	probably null	Het
Xrcc4	T	C	13: 90,140,594 (GRCm39)	E205G	possibly damaging	Het

Other mutations in Med12l

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00272:Med12l	APN	3	58,949,757 (GRCm39)	missense	probably damaging	0.98
IGL00561:Med12l	APN	3	59,135,245 (GRCm39)	missense	probably benign
IGL00974:Med12l	APN	3	58,990,435 (GRCm39)	missense	probably damaging	1.00
IGL01024:Med12l	APN	3	58,980,762 (GRCm39)	missense	probably damaging	1.00
IGL01094:Med12l	APN	3	59,001,076 (GRCm39)	missense	probably damaging	0.99
IGL01134:Med12l	APN	3	58,949,696 (GRCm39)	missense	possibly damaging	0.91
IGL01535:Med12l	APN	3	59,169,680 (GRCm39)	missense	probably damaging	1.00
IGL01653:Med12l	APN	3	59,169,314 (GRCm39)	missense	probably damaging	1.00
IGL01735:Med12l	APN	3	59,170,675 (GRCm39)	missense	probably damaging	1.00
IGL01972:Med12l	APN	3	59,169,314 (GRCm39)	missense	probably damaging	1.00
IGL02005:Med12l	APN	3	59,152,368 (GRCm39)	missense	probably damaging	1.00
IGL02098:Med12l	APN	3	59,183,276 (GRCm39)	missense	possibly damaging	0.92
IGL02115:Med12l	APN	3	58,975,740 (GRCm39)	missense	probably benign	0.00
IGL02231:Med12l	APN	3	59,153,303 (GRCm39)	missense	probably damaging	1.00
IGL02259:Med12l	APN	3	59,153,264 (GRCm39)	missense	probably damaging	1.00
IGL02369:Med12l	APN	3	59,164,794 (GRCm39)	missense	probably benign	0.00
IGL02424:Med12l	APN	3	59,000,143 (GRCm39)	missense	probably benign	0.21
IGL02501:Med12l	APN	3	59,169,397 (GRCm39)	missense	possibly damaging	0.71
IGL02525:Med12l	APN	3	58,975,789 (GRCm39)	missense	probably benign	0.01
IGL02530:Med12l	APN	3	58,984,510 (GRCm39)	missense	probably damaging	1.00
IGL02735:Med12l	APN	3	59,001,067 (GRCm39)	missense	probably damaging	1.00
IGL02865:Med12l	APN	3	59,201,713 (GRCm39)	missense	probably damaging	1.00
IGL03183:Med12l	APN	3	58,944,976 (GRCm39)	splice site	probably null
IGL03264:Med12l	APN	3	59,208,788 (GRCm39)	nonsense	probably null
FR4304:Med12l	UTSW	3	59,183,403 (GRCm39)	small insertion	probably benign
FR4340:Med12l	UTSW	3	59,183,406 (GRCm39)	small insertion	probably benign
FR4342:Med12l	UTSW	3	59,183,415 (GRCm39)	small insertion	probably benign
FR4342:Med12l	UTSW	3	59,183,409 (GRCm39)	small insertion	probably benign
FR4449:Med12l	UTSW	3	59,183,384 (GRCm39)	nonsense	probably null
FR4548:Med12l	UTSW	3	59,183,403 (GRCm39)	small insertion	probably benign
FR4589:Med12l	UTSW	3	59,183,377 (GRCm39)	small insertion	probably benign
FR4976:Med12l	UTSW	3	59,183,398 (GRCm39)	small insertion	probably benign
P0007:Med12l	UTSW	3	58,998,816 (GRCm39)	splice site	probably benign
P0045:Med12l	UTSW	3	58,998,956 (GRCm39)	missense	probably damaging	0.99
R0030:Med12l	UTSW	3	59,156,076 (GRCm39)	missense	probably damaging	1.00
R0030:Med12l	UTSW	3	59,156,076 (GRCm39)	missense	probably damaging	1.00
R0148:Med12l	UTSW	3	58,945,075 (GRCm39)	missense	probably damaging	1.00
R0325:Med12l	UTSW	3	58,984,480 (GRCm39)	missense	possibly damaging	0.88
R0330:Med12l	UTSW	3	59,135,123 (GRCm39)	missense	probably damaging	1.00
R0388:Med12l	UTSW	3	59,000,925 (GRCm39)	splice site	probably benign
R0542:Med12l	UTSW	3	58,949,822 (GRCm39)	missense	probably damaging	1.00
R0625:Med12l	UTSW	3	59,154,858 (GRCm39)	missense	probably damaging	1.00
R0671:Med12l	UTSW	3	59,172,350 (GRCm39)	missense	probably damaging	1.00
R0706:Med12l	UTSW	3	59,169,401 (GRCm39)	missense	probably damaging	1.00
R0785:Med12l	UTSW	3	59,168,253 (GRCm39)	missense	probably damaging	1.00
R1054:Med12l	UTSW	3	59,156,072 (GRCm39)	missense	probably damaging	0.99
R1102:Med12l	UTSW	3	59,152,257 (GRCm39)	missense	probably damaging	0.99
R1391:Med12l	UTSW	3	58,945,159 (GRCm39)	missense	probably benign	0.00
R1501:Med12l	UTSW	3	59,168,256 (GRCm39)	critical splice donor site	probably null
R1544:Med12l	UTSW	3	59,172,661 (GRCm39)	missense	possibly damaging	0.71
R1662:Med12l	UTSW	3	59,001,038 (GRCm39)	missense	probably damaging	1.00
R1670:Med12l	UTSW	3	59,183,379 (GRCm39)	small insertion	probably benign
R1839:Med12l	UTSW	3	58,975,740 (GRCm39)	missense	probably benign
R1854:Med12l	UTSW	3	59,168,193 (GRCm39)	missense	probably damaging	1.00
R2045:Med12l	UTSW	3	59,169,731 (GRCm39)	nonsense	probably null
R2070:Med12l	UTSW	3	59,152,326 (GRCm39)	missense	probably damaging	1.00
R2132:Med12l	UTSW	3	59,172,703 (GRCm39)	splice site	probably null
R2290:Med12l	UTSW	3	59,152,359 (GRCm39)	missense	probably damaging	1.00
R2325:Med12l	UTSW	3	59,139,875 (GRCm39)	missense	probably damaging	0.99
R2352:Med12l	UTSW	3	59,148,113 (GRCm39)	missense	probably damaging	1.00
R2484:Med12l	UTSW	3	59,205,259 (GRCm39)	missense	probably benign	0.18
R2906:Med12l	UTSW	3	59,164,503 (GRCm39)	missense	probably damaging	1.00
R3735:Med12l	UTSW	3	58,998,916 (GRCm39)	missense	probably damaging	1.00
R3736:Med12l	UTSW	3	58,998,916 (GRCm39)	missense	probably damaging	1.00
R3774:Med12l	UTSW	3	59,155,363 (GRCm39)	missense	probably damaging	0.97
R3957:Med12l	UTSW	3	58,980,589 (GRCm39)	missense	probably damaging	0.99
R4020:Med12l	UTSW	3	59,155,363 (GRCm39)	missense	probably damaging	0.97
R4087:Med12l	UTSW	3	59,205,342 (GRCm39)	missense	probably benign	0.00
R4231:Med12l	UTSW	3	59,164,644 (GRCm39)	splice site	probably null
R4233:Med12l	UTSW	3	59,164,644 (GRCm39)	splice site	probably null
R4235:Med12l	UTSW	3	59,164,644 (GRCm39)	splice site	probably null
R4236:Med12l	UTSW	3	59,164,644 (GRCm39)	splice site	probably null
R4327:Med12l	UTSW	3	59,172,688 (GRCm39)	missense	probably benign	0.01
R4328:Med12l	UTSW	3	59,172,688 (GRCm39)	missense	probably benign	0.01
R4346:Med12l	UTSW	3	58,938,976 (GRCm39)	missense	probably damaging	1.00
R4543:Med12l	UTSW	3	58,998,929 (GRCm39)	missense	probably damaging	1.00
R4559:Med12l	UTSW	3	58,914,523 (GRCm39)	critical splice donor site	probably null
R4776:Med12l	UTSW	3	59,140,633 (GRCm39)	missense	probably damaging	1.00
R4877:Med12l	UTSW	3	59,152,214 (GRCm39)	missense	probably damaging	1.00
R4983:Med12l	UTSW	3	59,169,350 (GRCm39)	missense	probably damaging	1.00
R5114:Med12l	UTSW	3	59,167,109 (GRCm39)	missense	possibly damaging	0.85
R5125:Med12l	UTSW	3	59,174,635 (GRCm39)	missense	possibly damaging	0.83
R5230:Med12l	UTSW	3	59,153,209 (GRCm39)	missense	probably damaging	1.00
R5407:Med12l	UTSW	3	59,165,622 (GRCm39)	missense	probably damaging	1.00
R5426:Med12l	UTSW	3	59,156,143 (GRCm39)	missense	probably damaging	0.98
R5439:Med12l	UTSW	3	59,170,634 (GRCm39)	missense	probably null	1.00
R5449:Med12l	UTSW	3	59,167,127 (GRCm39)	missense	probably damaging	1.00
R5596:Med12l	UTSW	3	59,159,771 (GRCm39)	missense	probably benign	0.45
R5716:Med12l	UTSW	3	59,208,798 (GRCm39)	critical splice donor site	probably null
R5833:Med12l	UTSW	3	59,172,647 (GRCm39)	missense	possibly damaging	0.95
R5883:Med12l	UTSW	3	58,998,889 (GRCm39)	missense	probably damaging	1.00
R6264:Med12l	UTSW	3	59,163,423 (GRCm39)	missense	probably damaging	1.00
R6269:Med12l	UTSW	3	59,135,243 (GRCm39)	missense	probably damaging	1.00
R6394:Med12l	UTSW	3	59,142,508 (GRCm39)	missense	probably damaging	1.00
R6400:Med12l	UTSW	3	59,155,332 (GRCm39)	missense	probably damaging	1.00
R6475:Med12l	UTSW	3	59,164,500 (GRCm39)	missense	probably damaging	1.00
R6489:Med12l	UTSW	3	59,164,828 (GRCm39)	missense	probably damaging	0.99
R6654:Med12l	UTSW	3	59,169,713 (GRCm39)	missense	probably damaging	1.00
R6881:Med12l	UTSW	3	59,174,586 (GRCm39)	missense	probably benign	0.00
R7110:Med12l	UTSW	3	59,169,645 (GRCm39)	missense	possibly damaging	0.92
R7134:Med12l	UTSW	3	59,001,180 (GRCm39)	nonsense	probably null
R7137:Med12l	UTSW	3	59,165,675 (GRCm39)	missense	probably damaging	1.00
R7159:Med12l	UTSW	3	59,183,438 (GRCm39)	missense	probably benign
R7341:Med12l	UTSW	3	58,949,824 (GRCm39)	missense	possibly damaging	0.53
R7349:Med12l	UTSW	3	59,165,746 (GRCm39)	missense	probably damaging	1.00
R7413:Med12l	UTSW	3	58,998,971 (GRCm39)	missense	probably benign	0.00
R7495:Med12l	UTSW	3	59,152,194 (GRCm39)	missense	probably damaging	1.00
R7678:Med12l	UTSW	3	58,984,141 (GRCm39)	missense	probably damaging	1.00
R7697:Med12l	UTSW	3	59,148,078 (GRCm39)	missense	probably damaging	1.00
R7714:Med12l	UTSW	3	59,001,007 (GRCm39)	missense	probably benign	0.17
R7725:Med12l	UTSW	3	59,163,413 (GRCm39)	missense	probably damaging	1.00
R7846:Med12l	UTSW	3	59,172,355 (GRCm39)	missense	probably damaging	1.00
R7852:Med12l	UTSW	3	59,155,332 (GRCm39)	missense	probably damaging	1.00
R8080:Med12l	UTSW	3	59,172,607 (GRCm39)	missense	probably damaging	1.00
R8181:Med12l	UTSW	3	59,169,389 (GRCm39)	missense	probably damaging	1.00
R8223:Med12l	UTSW	3	58,993,784 (GRCm39)	missense	possibly damaging	0.79
R8560:Med12l	UTSW	3	58,945,026 (GRCm39)	missense	probably damaging	1.00
R8708:Med12l	UTSW	3	59,159,751 (GRCm39)	missense	probably benign	0.00
R8865:Med12l	UTSW	3	58,979,303 (GRCm39)	missense	probably benign
R8947:Med12l	UTSW	3	58,984,443 (GRCm39)	splice site	probably benign
R8976:Med12l	UTSW	3	59,183,329 (GRCm39)	missense	probably damaging	0.99
R9016:Med12l	UTSW	3	59,163,294 (GRCm39)	missense	probably damaging	0.96
R9183:Med12l	UTSW	3	58,984,498 (GRCm39)	missense	probably damaging	1.00
R9487:Med12l	UTSW	3	59,155,353 (GRCm39)	missense	probably benign
R9526:Med12l	UTSW	3	58,984,207 (GRCm39)	missense	probably damaging	0.96
R9802:Med12l	UTSW	3	59,169,346 (GRCm39)	missense	probably damaging	1.00
RF004:Med12l	UTSW	3	59,183,390 (GRCm39)	small insertion	probably benign
RF011:Med12l	UTSW	3	59,183,401 (GRCm39)	small insertion	probably benign
RF013:Med12l	UTSW	3	59,183,387 (GRCm39)	small insertion	probably benign
RF020:Med12l	UTSW	3	59,183,379 (GRCm39)	small insertion	probably benign
RF021:Med12l	UTSW	3	58,980,711 (GRCm39)	missense	probably benign	0.19
RF027:Med12l	UTSW	3	59,183,402 (GRCm39)	small insertion	probably benign
RF027:Med12l	UTSW	3	59,183,388 (GRCm39)	small insertion	probably benign
RF030:Med12l	UTSW	3	59,183,410 (GRCm39)	small insertion	probably benign
RF032:Med12l	UTSW	3	59,183,410 (GRCm39)	small insertion	probably benign
RF032:Med12l	UTSW	3	59,183,406 (GRCm39)	small insertion	probably benign
RF032:Med12l	UTSW	3	59,183,402 (GRCm39)	small insertion	probably benign
RF033:Med12l	UTSW	3	59,183,416 (GRCm39)	small insertion	probably benign
RF033:Med12l	UTSW	3	59,183,408 (GRCm39)	small insertion	probably benign
RF033:Med12l	UTSW	3	59,183,402 (GRCm39)	small insertion	probably benign
RF037:Med12l	UTSW	3	59,183,377 (GRCm39)	small insertion	probably benign
RF040:Med12l	UTSW	3	59,183,410 (GRCm39)	small insertion	probably benign
RF040:Med12l	UTSW	3	59,183,388 (GRCm39)	small insertion	probably benign
RF041:Med12l	UTSW	3	59,183,416 (GRCm39)	small insertion	probably benign
RF041:Med12l	UTSW	3	59,183,406 (GRCm39)	small insertion	probably benign
RF042:Med12l	UTSW	3	59,183,402 (GRCm39)	small insertion	probably benign
RF042:Med12l	UTSW	3	59,183,388 (GRCm39)	small insertion	probably benign
RF042:Med12l	UTSW	3	59,183,377 (GRCm39)	small insertion	probably benign
RF042:Med12l	UTSW	3	59,183,416 (GRCm39)	small insertion	probably benign
RF049:Med12l	UTSW	3	59,183,390 (GRCm39)	small insertion	probably benign
RF050:Med12l	UTSW	3	59,183,394 (GRCm39)	small insertion	probably benign
RF053:Med12l	UTSW	3	59,183,414 (GRCm39)	small insertion	probably benign
RF055:Med12l	UTSW	3	59,183,404 (GRCm39)	small insertion	probably benign
RF056:Med12l	UTSW	3	59,183,414 (GRCm39)	small insertion	probably benign
RF057:Med12l	UTSW	3	59,183,401 (GRCm39)	small insertion	probably benign
RF063:Med12l	UTSW	3	59,183,394 (GRCm39)	small insertion	probably benign
RF063:Med12l	UTSW	3	59,183,379 (GRCm39)	small insertion	probably benign
X0062:Med12l	UTSW	3	59,140,600 (GRCm39)	missense	probably damaging	1.00
Z1176:Med12l	UTSW	3	59,203,538 (GRCm39)	missense	probably benign	0.00
Z1176:Med12l	UTSW	3	59,152,364 (GRCm39)	missense	probably damaging	1.00
Z1176:Med12l	UTSW	3	58,998,838 (GRCm39)	missense	probably damaging	0.98
Z1177:Med12l	UTSW	3	59,155,296 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TTCTGAAGAAGGAGAGCATGGCCC -3'
(R):5'- GGCAGTATGTGCCTACAACTGAACC -3'

Sequencing Primer
(F):5'- ACTATGTAACACTGAGGCCGTTG -3'
(R):5'- ACCTTTGGTTTAAAAAAGAGTGGG -3'

Posted On

2013-07-11