Incidental Mutation 'R0689:Vmn2r77'
ID 61278
Institutional Source Beutler Lab
Gene Symbol Vmn2r77
Ensembl Gene ENSMUSG00000090949
Gene Name vomeronasal 2, receptor 77
Synonyms EG546983
MMRRC Submission 038874-MU
Accession Numbers
Essential gene? Probably non essential (E-score: 0.079) question?
Stock # R0689 (G1)
Quality Score 147
Status Validated
Chromosome 7
Chromosomal Location 86444349-86461240 bp(+) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) A to T at 86460872 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Isoleucine to Phenylalanine at position 733 (I733F)
Ref Sequence ENSEMBL: ENSMUSP00000129540 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000164996]
AlphaFold L7N2B7
Predicted Effect probably damaging
Transcript: ENSMUST00000164996
AA Change: I733F

PolyPhen 2 Score 0.993 (Sensitivity: 0.70; Specificity: 0.97)
SMART Domains Protein: ENSMUSP00000129540
Gene: ENSMUSG00000090949
AA Change: I733F

DomainStartEndE-ValueType
signal peptide 1 21 N/A INTRINSIC
Pfam:ANF_receptor 78 467 1.4e-30 PFAM
Pfam:NCD3G 510 562 1e-20 PFAM
Pfam:7tm_3 594 830 2.6e-52 PFAM
Meta Mutation Damage Score 0.6467 question?
Coding Region Coverage
  • 1x: 99.2%
  • 3x: 98.5%
  • 10x: 96.4%
  • 20x: 91.1%
Validation Efficiency 99% (80/81)
Allele List at MGI
Other mutations in this stock
Total: 64 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Actn4 C A 7: 28,596,474 (GRCm39) G674W probably damaging Het
Adcy9 A G 16: 4,130,668 (GRCm39) probably benign Het
Adgrv1 C T 13: 81,623,224 (GRCm39) V3800I possibly damaging Het
Agl A G 3: 116,587,277 (GRCm39) Y93H probably damaging Het
Aldh1a3 T A 7: 66,051,753 (GRCm39) D400V probably benign Het
Bpifc A G 10: 85,796,411 (GRCm39) probably benign Het
Cachd1 G T 4: 100,832,073 (GRCm39) R745L probably damaging Het
Cadm3 T A 1: 173,172,019 (GRCm39) T185S possibly damaging Het
Cep85l G T 10: 53,224,943 (GRCm39) D215E probably damaging Het
Ces1g A G 8: 94,055,035 (GRCm39) S221P probably damaging Het
Cfap206 C T 4: 34,722,668 (GRCm39) V138M probably benign Het
Csmd3 A T 15: 47,619,421 (GRCm39) F1714I probably benign Het
Cyp4f18 A G 8: 72,749,812 (GRCm39) L279P probably benign Het
Dnah7a G A 1: 53,659,840 (GRCm39) Q723* probably null Het
Dnaja2 A G 8: 86,273,347 (GRCm39) probably benign Het
Dnajc6 T C 4: 101,468,450 (GRCm39) V162A possibly damaging Het
Dok4 T C 8: 95,597,547 (GRCm39) T3A probably benign Het
Efcab7 T C 4: 99,761,981 (GRCm39) W424R probably damaging Het
Fah A T 7: 84,242,392 (GRCm39) probably null Het
Fam120a T C 13: 49,121,114 (GRCm39) D64G probably damaging Het
G3bp1 T C 11: 55,389,452 (GRCm39) F383L probably damaging Het
Gas8 T G 8: 124,250,845 (GRCm39) L106R probably damaging Het
Gykl1 T G 18: 52,827,123 (GRCm39) N110K possibly damaging Het
Hsd3b3 G T 3: 98,649,295 (GRCm39) L343I possibly damaging Het
Itch T A 2: 155,024,098 (GRCm39) S234T possibly damaging Het
Itgbl1 A T 14: 124,065,259 (GRCm39) I61F possibly damaging Het
Klf6 T A 13: 5,915,115 (GRCm39) S185T probably damaging Het
Klk1b1 A C 7: 43,620,143 (GRCm39) K202T probably benign Het
Liph T C 16: 21,786,818 (GRCm39) Y268C probably damaging Het
Mroh2a C T 1: 88,158,402 (GRCm39) R150* probably null Het
Mroh2a A G 1: 88,186,386 (GRCm39) S64G probably benign Het
Myo9b G A 8: 71,783,400 (GRCm39) D574N probably damaging Het
Nfyb G A 10: 82,590,836 (GRCm39) A65V possibly damaging Het
Nipbl A G 15: 8,322,562 (GRCm39) probably null Het
Olfm3 T A 3: 114,916,194 (GRCm39) N355K probably benign Het
Or4k15c A T 14: 50,321,689 (GRCm39) F150I probably benign Het
Pcdhb21 A G 18: 37,648,370 (GRCm39) T500A probably benign Het
Pclo A G 5: 14,764,033 (GRCm39) I4169V unknown Het
Pde4d T C 13: 109,877,078 (GRCm39) S144P possibly damaging Het
Pgghg T C 7: 140,523,191 (GRCm39) Y157H probably benign Het
Pkd1l3 C G 8: 110,350,281 (GRCm39) D375E possibly damaging Het
Pla2g12a T C 3: 129,674,947 (GRCm39) probably null Het
Ppp1r14d T C 2: 119,060,093 (GRCm39) D63G probably damaging Het
Sema6a G A 18: 47,423,112 (GRCm39) probably null Het
Sgip1 A T 4: 102,823,449 (GRCm39) D690V probably damaging Het
Skp1 T C 11: 52,134,592 (GRCm39) probably benign Het
Slc25a12 T C 2: 71,141,837 (GRCm39) Y272C possibly damaging Het
Slc37a2 A G 9: 37,146,846 (GRCm39) probably benign Het
Snx6 A T 12: 54,810,441 (GRCm39) S112T probably benign Het
Sox6 A G 7: 115,085,786 (GRCm39) V685A probably damaging Het
Taf2 A T 15: 54,926,461 (GRCm39) V163E possibly damaging Het
Tg A T 15: 66,711,253 (GRCm39) probably benign Het
Tmem30c A G 16: 57,090,536 (GRCm39) Y224H probably damaging Het
Tmem45b T C 9: 31,339,879 (GRCm39) N173D probably benign Het
Traf5 T C 1: 191,729,837 (GRCm39) T405A probably benign Het
Trerf1 C T 17: 47,630,300 (GRCm39) noncoding transcript Het
Triobp A T 15: 78,844,188 (GRCm39) K135* probably null Het
Ttll9 T A 2: 152,825,047 (GRCm39) D75E probably benign Het
Vmn1r63 T C 7: 5,806,609 (GRCm39) I8V probably benign Het
Vmn2r98 T C 17: 19,300,782 (GRCm39) S595P possibly damaging Het
Zcchc2 C T 1: 105,958,234 (GRCm39) Q504* probably null Het
Zfp2 T C 11: 50,791,734 (GRCm39) D103G probably benign Het
Zfp59 A G 7: 27,553,142 (GRCm39) K198R probably benign Het
Zfp64 C A 2: 168,777,121 (GRCm39) probably benign Het
Other mutations in Vmn2r77
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL00954:Vmn2r77 APN 7 86,449,975 (GRCm39) missense probably benign 0.06
IGL01105:Vmn2r77 APN 7 86,460,872 (GRCm39) missense probably damaging 0.99
IGL01367:Vmn2r77 APN 7 86,461,124 (GRCm39) missense probably damaging 0.98
IGL01634:Vmn2r77 APN 7 86,460,857 (GRCm39) missense probably benign
IGL01805:Vmn2r77 APN 7 86,460,395 (GRCm39) missense probably benign 0.18
IGL01868:Vmn2r77 APN 7 86,452,224 (GRCm39) missense probably benign 0.00
IGL01980:Vmn2r77 APN 7 86,450,678 (GRCm39) missense probably benign 0.14
IGL02055:Vmn2r77 APN 7 86,450,763 (GRCm39) missense probably benign 0.00
IGL02066:Vmn2r77 APN 7 86,452,836 (GRCm39) nonsense probably null
IGL02185:Vmn2r77 APN 7 86,444,360 (GRCm39) missense unknown
IGL02200:Vmn2r77 APN 7 86,451,187 (GRCm39) missense probably benign 0.04
IGL02336:Vmn2r77 APN 7 86,451,224 (GRCm39) missense probably damaging 0.99
IGL02445:Vmn2r77 APN 7 86,452,848 (GRCm39) nonsense probably null
IGL02557:Vmn2r77 APN 7 86,444,342 (GRCm39) unclassified probably benign
IGL02659:Vmn2r77 APN 7 86,449,979 (GRCm39) missense probably benign 0.32
IGL02978:Vmn2r77 APN 7 86,460,555 (GRCm39) missense probably benign
IGL03180:Vmn2r77 APN 7 86,450,843 (GRCm39) missense possibly damaging 0.85
IGL03255:Vmn2r77 APN 7 86,461,131 (GRCm39) missense probably benign 0.04
IGL03273:Vmn2r77 APN 7 86,460,494 (GRCm39) missense probably damaging 0.99
R0046:Vmn2r77 UTSW 7 86,451,146 (GRCm39) missense possibly damaging 0.73
R0047:Vmn2r77 UTSW 7 86,460,858 (GRCm39) missense probably benign 0.01
R0066:Vmn2r77 UTSW 7 86,449,964 (GRCm39) missense probably benign 0.17
R0066:Vmn2r77 UTSW 7 86,449,964 (GRCm39) missense probably benign 0.17
R0389:Vmn2r77 UTSW 7 86,450,702 (GRCm39) missense probably benign 0.29
R0635:Vmn2r77 UTSW 7 86,460,383 (GRCm39) missense probably benign
R0827:Vmn2r77 UTSW 7 86,451,224 (GRCm39) missense probably damaging 1.00
R1167:Vmn2r77 UTSW 7 86,450,954 (GRCm39) missense probably benign 0.02
R1228:Vmn2r77 UTSW 7 86,450,242 (GRCm39) critical splice donor site probably null
R1353:Vmn2r77 UTSW 7 86,451,394 (GRCm39) missense probably benign 0.29
R1392:Vmn2r77 UTSW 7 86,450,830 (GRCm39) missense probably benign 0.00
R1392:Vmn2r77 UTSW 7 86,450,830 (GRCm39) missense probably benign 0.00
R1613:Vmn2r77 UTSW 7 86,460,356 (GRCm39) missense probably damaging 1.00
R1654:Vmn2r77 UTSW 7 86,461,123 (GRCm39) missense probably damaging 1.00
R1742:Vmn2r77 UTSW 7 86,444,543 (GRCm39) missense probably benign 0.35
R1827:Vmn2r77 UTSW 7 86,450,821 (GRCm39) missense probably damaging 0.99
R1911:Vmn2r77 UTSW 7 86,461,001 (GRCm39) missense probably damaging 1.00
R1974:Vmn2r77 UTSW 7 86,449,964 (GRCm39) missense probably benign 0.17
R2008:Vmn2r77 UTSW 7 86,450,921 (GRCm39) missense probably benign 0.31
R2093:Vmn2r77 UTSW 7 86,450,702 (GRCm39) missense probably benign 0.29
R2143:Vmn2r77 UTSW 7 86,461,152 (GRCm39) missense probably damaging 1.00
R2269:Vmn2r77 UTSW 7 86,460,897 (GRCm39) missense probably benign 0.03
R2972:Vmn2r77 UTSW 7 86,452,893 (GRCm39) missense probably benign 0.01
R2974:Vmn2r77 UTSW 7 86,452,893 (GRCm39) missense probably benign 0.01
R3037:Vmn2r77 UTSW 7 86,450,191 (GRCm39) missense probably benign
R3694:Vmn2r77 UTSW 7 86,450,044 (GRCm39) missense probably damaging 1.00
R3695:Vmn2r77 UTSW 7 86,450,044 (GRCm39) missense probably damaging 1.00
R3805:Vmn2r77 UTSW 7 86,444,368 (GRCm39) nonsense probably null
R3870:Vmn2r77 UTSW 7 86,461,050 (GRCm39) missense probably damaging 1.00
R4732:Vmn2r77 UTSW 7 86,450,195 (GRCm39) missense probably benign 0.00
R4733:Vmn2r77 UTSW 7 86,450,195 (GRCm39) missense probably benign 0.00
R5009:Vmn2r77 UTSW 7 86,451,015 (GRCm39) missense possibly damaging 0.82
R5201:Vmn2r77 UTSW 7 86,460,846 (GRCm39) missense probably damaging 0.98
R5218:Vmn2r77 UTSW 7 86,451,341 (GRCm39) missense probably damaging 0.98
R5469:Vmn2r77 UTSW 7 86,451,271 (GRCm39) missense probably benign 0.01
R5673:Vmn2r77 UTSW 7 86,461,214 (GRCm39) missense probably benign 0.05
R5771:Vmn2r77 UTSW 7 86,461,235 (GRCm39) missense probably benign 0.06
R5832:Vmn2r77 UTSW 7 86,460,670 (GRCm39) nonsense probably null
R5899:Vmn2r77 UTSW 7 86,460,924 (GRCm39) missense probably damaging 1.00
R6151:Vmn2r77 UTSW 7 86,450,878 (GRCm39) missense probably benign 0.00
R6182:Vmn2r77 UTSW 7 86,460,957 (GRCm39) missense probably damaging 1.00
R6326:Vmn2r77 UTSW 7 86,451,031 (GRCm39) missense probably benign
R6419:Vmn2r77 UTSW 7 86,460,767 (GRCm39) missense probably damaging 0.99
R6549:Vmn2r77 UTSW 7 86,450,065 (GRCm39) missense probably benign 0.06
R6874:Vmn2r77 UTSW 7 86,451,286 (GRCm39) missense probably benign 0.00
R6972:Vmn2r77 UTSW 7 86,452,202 (GRCm39) missense probably damaging 1.00
R7056:Vmn2r77 UTSW 7 86,451,023 (GRCm39) missense probably benign 0.06
R7185:Vmn2r77 UTSW 7 86,451,035 (GRCm39) missense probably benign 0.00
R7261:Vmn2r77 UTSW 7 86,460,518 (GRCm39) nonsense probably null
R7298:Vmn2r77 UTSW 7 86,449,979 (GRCm39) missense probably benign 0.00
R7662:Vmn2r77 UTSW 7 86,460,492 (GRCm39) nonsense probably null
R8182:Vmn2r77 UTSW 7 86,460,801 (GRCm39) missense probably damaging 1.00
R8327:Vmn2r77 UTSW 7 86,450,680 (GRCm39) missense probably benign 0.08
R8387:Vmn2r77 UTSW 7 86,450,947 (GRCm39) missense probably benign 0.00
R8825:Vmn2r77 UTSW 7 86,452,855 (GRCm39) missense probably benign
R8898:Vmn2r77 UTSW 7 86,444,430 (GRCm39) missense probably damaging 1.00
R8973:Vmn2r77 UTSW 7 86,452,150 (GRCm39) missense possibly damaging 0.93
R9258:Vmn2r77 UTSW 7 86,452,302 (GRCm39) missense possibly damaging 0.88
R9338:Vmn2r77 UTSW 7 86,460,994 (GRCm39) missense probably damaging 1.00
R9358:Vmn2r77 UTSW 7 86,452,236 (GRCm39) missense probably benign 0.00
R9377:Vmn2r77 UTSW 7 86,444,442 (GRCm39) missense probably benign 0.05
R9404:Vmn2r77 UTSW 7 86,451,247 (GRCm39) missense probably benign
R9673:Vmn2r77 UTSW 7 86,450,171 (GRCm39) missense possibly damaging 0.75
R9679:Vmn2r77 UTSW 7 86,460,741 (GRCm39) missense probably benign 0.07
Predicted Primers PCR Primer
(F):5'- TGCAACAGACCACATTTGCCATTG -3'
(R):5'- TCCCTGCACTGGAGGCTAAGATAC -3'

Sequencing Primer
(F):5'- CTGTAGTCCTAGCATTTAAGATCAC -3'
(R):5'- AACCATAGCCTTACCCTTAGTG -3'
Posted On 2013-07-30