Mutagenetix > Incidental Mutation

Incidental Mutation 'R0661:Vmn2r75'

61840

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r75

Ensembl Gene

ENSMUSG00000090436

Gene Name

vomeronasal 2, receptor 75

Synonyms

EG546981

MMRRC Submission

038846-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.090) question?

Stock #

R0661 (G1)

Quality Score

145

Status

Not validated

Chromosome

Chromosomal Location

85797250-85820932 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

G to A at 85814866 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Alanine to Valine at position 209 (A209V)

Ref Sequence

ENSEMBL: ENSMUSP00000126973 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000167830]

AlphaFold

G5E8Z7

Predicted Effect

probably benign
Transcript: ENSMUST00000167830
AA Change: A209V

PolyPhen 2 Score 0.002 (Sensitivity: 0.99; Specificity: 0.30)

SMART Domains

Protein: ENSMUSP00000126973
Gene: ENSMUSG00000090436
AA Change: A209V

Domain	Start	End	E-Value	Type
signal peptide	1	21	N/A	INTRINSIC
Pfam:ANF_receptor	80	466	2.8e-31	PFAM
Pfam:NCD3G	510	562	4.6e-20	PFAM
Pfam:7tm_3	593	829	7.7e-51	PFAM

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 99.4%
3x: 98.9%
10x: 97.5%
20x: 95.0%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 41 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Agtr1b	T	A	3: 20,370,163 (GRCm39)	T148S	possibly damaging	Het
Anks3	A	G	16: 4,766,198 (GRCm39)	F124L	probably damaging	Het
Ar	T	A	X: 97,194,171 (GRCm39)	Y262N	probably damaging	Het
Asxl1	T	A	2: 153,242,644 (GRCm39)	S1065T	possibly damaging	Het
Brip1	A	T	11: 86,001,189 (GRCm39)	I749N	possibly damaging	Het
C1ra	T	A	6: 124,499,336 (GRCm39)	H507Q	probably benign	Het
Cdk9	G	A	2: 32,599,832 (GRCm39)	T135I	probably damaging	Het
Col1a1	A	G	11: 94,840,215 (GRCm39)	T1088A	unknown	Het
Cpne2	T	C	8: 95,282,667 (GRCm39)	I283T	possibly damaging	Het
Dcaf17	T	C	2: 70,918,779 (GRCm39)	L451P	probably damaging	Het
Dhx57	C	T	17: 80,576,293 (GRCm39)	C599Y	probably damaging	Het
Drd1	T	A	13: 54,207,057 (GRCm39)	N379Y	possibly damaging	Het
Fsip2	A	G	2: 82,816,513 (GRCm39)	D4082G	possibly damaging	Het
Grin2a	G	T	16: 9,810,336 (GRCm39)	P21Q	probably damaging	Het
Heyl	G	T	4: 123,139,824 (GRCm39)	V128F	probably damaging	Het
Hoxd12	A	G	2: 74,506,236 (GRCm39)	E216G	probably damaging	Het
Inpp4b	C	A	8: 82,468,091 (GRCm39)	A18E	possibly damaging	Het
Invs	G	A	4: 48,421,861 (GRCm39)	R831H	probably benign	Het
Lrrk2	T	C	15: 91,671,219 (GRCm39)	V2000A	probably damaging	Het
Msh3	T	C	13: 92,481,604 (GRCm39)	N303D	possibly damaging	Het
Or11g27	A	G	14: 50,771,552 (GRCm39)	T228A	probably benign	Het
Or5an9	T	C	19: 12,187,068 (GRCm39)	L46P	probably damaging	Het
Or5b105	G	A	19: 13,080,642 (GRCm39)	R3C	possibly damaging	Het
Pcdh18	A	C	3: 49,707,767 (GRCm39)	S902R	possibly damaging	Het
Prdm15	A	T	16: 97,630,882 (GRCm39)	V190E	probably benign	Het
Ranbp2	T	G	10: 58,314,555 (GRCm39)	S1758R	probably benign	Het
Rimbp2	A	G	5: 128,863,774 (GRCm39)	V738A	probably benign	Het
Rtl5	T	C	X: 101,114,056 (GRCm39)	H138R	possibly damaging	Het
Sec11a	A	G	7: 80,584,787 (GRCm39)	V50A	probably damaging	Het
Shroom1	T	C	11: 53,357,764 (GRCm39)	S772P	possibly damaging	Het
Slc26a6	T	C	9: 108,736,312 (GRCm39)		probably null	Het
Slf1	A	G	13: 77,231,715 (GRCm39)	W555R	probably benign	Het
Spx	A	G	6: 142,359,565 (GRCm39)	S5G	possibly damaging	Het
Tcp1	T	C	17: 13,142,200 (GRCm39)	V398A	probably benign	Het
Tm6sf1	G	A	7: 81,515,093 (GRCm39)		probably null	Het
Tsbp1	A	T	17: 34,678,887 (GRCm39)	I217F	possibly damaging	Het
Ufsp2	T	A	8: 46,432,270 (GRCm39)	M1K	probably null	Het
Usf1	G	A	1: 171,245,067 (GRCm39)	R196Q	probably damaging	Het
Yme1l1	T	A	2: 23,081,054 (GRCm39)	M442K	probably damaging	Het
Zfand3	A	G	17: 30,354,372 (GRCm39)	E63G	probably damaging	Het
Zfp740	A	G	15: 102,121,094 (GRCm39)	T136A	possibly damaging	Het

Other mutations in Vmn2r75

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01133:Vmn2r75	APN	7	85,797,240 (GRCm39)	unclassified	probably benign
IGL01287:Vmn2r75	APN	7	85,797,801 (GRCm39)	missense	probably damaging	0.97
IGL01318:Vmn2r75	APN	7	85,814,774 (GRCm39)	missense	probably benign	0.06
IGL01331:Vmn2r75	APN	7	85,820,870 (GRCm39)	nonsense	probably null
IGL01406:Vmn2r75	APN	7	85,812,500 (GRCm39)	splice site	probably benign
IGL01615:Vmn2r75	APN	7	85,797,681 (GRCm39)	missense	probably benign	0.03
IGL01657:Vmn2r75	APN	7	85,813,455 (GRCm39)	missense	probably damaging	1.00
IGL02237:Vmn2r75	APN	7	85,814,786 (GRCm39)	missense	possibly damaging	0.88
IGL02275:Vmn2r75	APN	7	85,814,348 (GRCm39)	missense	probably benign	0.04
IGL02307:Vmn2r75	APN	7	85,814,974 (GRCm39)	missense	probably benign	0.00
IGL03136:Vmn2r75	APN	7	85,797,911 (GRCm39)	missense	possibly damaging	0.89
IGL03160:Vmn2r75	APN	7	85,797,644 (GRCm39)	missense	probably damaging	1.00
IGL03244:Vmn2r75	APN	7	85,820,933 (GRCm39)	unclassified	probably benign
PIT4449001:Vmn2r75	UTSW	7	85,814,791 (GRCm39)	missense	probably damaging	1.00
R0049:Vmn2r75	UTSW	7	85,797,309 (GRCm39)	nonsense	probably null
R0049:Vmn2r75	UTSW	7	85,797,309 (GRCm39)	nonsense	probably null
R0083:Vmn2r75	UTSW	7	85,814,866 (GRCm39)	missense	probably benign	0.00
R0108:Vmn2r75	UTSW	7	85,814,866 (GRCm39)	missense	probably benign	0.00
R0276:Vmn2r75	UTSW	7	85,797,515 (GRCm39)	missense	probably benign	0.01
R0320:Vmn2r75	UTSW	7	85,814,288 (GRCm39)	missense	probably benign	0.36
R0471:Vmn2r75	UTSW	7	85,814,721 (GRCm39)	missense	probably benign	0.01
R0562:Vmn2r75	UTSW	7	85,797,449 (GRCm39)	nonsense	probably null
R0631:Vmn2r75	UTSW	7	85,812,478 (GRCm39)	missense	probably null	1.00
R0811:Vmn2r75	UTSW	7	85,814,575 (GRCm39)	missense	probably benign	0.38
R0812:Vmn2r75	UTSW	7	85,814,575 (GRCm39)	missense	probably benign	0.38
R0891:Vmn2r75	UTSW	7	85,813,476 (GRCm39)	missense	possibly damaging	0.81
R1340:Vmn2r75	UTSW	7	85,797,798 (GRCm39)	missense	probably damaging	0.98
R1501:Vmn2r75	UTSW	7	85,814,850 (GRCm39)	missense	possibly damaging	0.85
R1760:Vmn2r75	UTSW	7	85,798,019 (GRCm39)	missense	probably damaging	1.00
R1970:Vmn2r75	UTSW	7	85,797,470 (GRCm39)	missense	probably damaging	1.00
R2060:Vmn2r75	UTSW	7	85,814,372 (GRCm39)	missense	probably benign	0.00
R2292:Vmn2r75	UTSW	7	85,798,144 (GRCm39)	missense	probably damaging	1.00
R3688:Vmn2r75	UTSW	7	85,797,629 (GRCm39)	missense	probably damaging	0.99
R3892:Vmn2r75	UTSW	7	85,813,494 (GRCm39)	missense	probably null	1.00
R4532:Vmn2r75	UTSW	7	85,797,349 (GRCm39)	nonsense	probably null
R4583:Vmn2r75	UTSW	7	85,813,290 (GRCm39)	missense	possibly damaging	0.81
R4592:Vmn2r75	UTSW	7	85,815,494 (GRCm39)	missense	probably benign	0.00
R4792:Vmn2r75	UTSW	7	85,812,378 (GRCm39)	missense	possibly damaging	0.46
R4859:Vmn2r75	UTSW	7	85,797,611 (GRCm39)	missense	probably benign	0.35
R4896:Vmn2r75	UTSW	7	85,820,787 (GRCm39)	missense	probably benign	0.01
R4943:Vmn2r75	UTSW	7	85,814,705 (GRCm39)	missense	probably damaging	1.00
R4992:Vmn2r75	UTSW	7	85,815,375 (GRCm39)	critical splice donor site	probably null
R5048:Vmn2r75	UTSW	7	85,814,735 (GRCm39)	missense	possibly damaging	0.66
R5063:Vmn2r75	UTSW	7	85,813,372 (GRCm39)	missense	probably benign
R5156:Vmn2r75	UTSW	7	85,813,436 (GRCm39)	missense	possibly damaging	0.51
R5243:Vmn2r75	UTSW	7	85,813,447 (GRCm39)	missense	probably damaging	1.00
R5277:Vmn2r75	UTSW	7	85,815,500 (GRCm39)	missense	probably benign
R5574:Vmn2r75	UTSW	7	85,815,510 (GRCm39)	missense	probably benign	0.22
R5622:Vmn2r75	UTSW	7	85,797,702 (GRCm39)	missense	probably benign	0.15
R5680:Vmn2r75	UTSW	7	85,820,779 (GRCm39)	missense	probably benign	0.10
R5884:Vmn2r75	UTSW	7	85,814,578 (GRCm39)	missense	probably benign
R6021:Vmn2r75	UTSW	7	85,820,820 (GRCm39)	missense	probably benign	0.01
R6217:Vmn2r75	UTSW	7	85,815,375 (GRCm39)	critical splice donor site	probably benign
R6242:Vmn2r75	UTSW	7	85,814,592 (GRCm39)	missense	probably damaging	1.00
R6299:Vmn2r75	UTSW	7	85,814,482 (GRCm39)	missense	probably benign	0.12
R6441:Vmn2r75	UTSW	7	85,820,784 (GRCm39)	missense	probably damaging	0.99
R6495:Vmn2r75	UTSW	7	85,813,287 (GRCm39)	missense	probably benign	0.00
R6553:Vmn2r75	UTSW	7	85,813,453 (GRCm39)	missense	probably benign	0.28
R6670:Vmn2r75	UTSW	7	85,797,644 (GRCm39)	missense	probably damaging	1.00
R7078:Vmn2r75	UTSW	7	85,815,568 (GRCm39)	missense	probably damaging	1.00
R7164:Vmn2r75	UTSW	7	85,814,592 (GRCm39)	missense	probably damaging	1.00
R8411:Vmn2r75	UTSW	7	85,797,722 (GRCm39)	missense	probably damaging	1.00
R8507:Vmn2r75	UTSW	7	85,797,685 (GRCm39)	nonsense	probably null
R8559:Vmn2r75	UTSW	7	85,815,480 (GRCm39)	missense	possibly damaging	0.65
R8677:Vmn2r75	UTSW	7	85,814,410 (GRCm39)	missense	possibly damaging	0.86
R8708:Vmn2r75	UTSW	7	85,812,476 (GRCm39)	missense	probably damaging	0.99
R8778:Vmn2r75	UTSW	7	85,813,497 (GRCm39)	missense	probably benign	0.40
R8968:Vmn2r75	UTSW	7	85,820,765 (GRCm39)	nonsense	probably null
R9145:Vmn2r75	UTSW	7	85,813,447 (GRCm39)	missense	probably damaging	1.00
R9316:Vmn2r75	UTSW	7	85,797,313 (GRCm39)	missense	possibly damaging	0.63
R9363:Vmn2r75	UTSW	7	85,815,423 (GRCm39)	missense	probably benign	0.03

Predicted Primers

PCR Primer
(F):5'- ACCCAGATTCTCCAAATGCCTTCAG -3'
(R):5'- GCATTGATACACTTTGCTCAAGCACAC -3'

Sequencing Primer
(F):5'- GGGAGAGTCTTTGTCTCCATAAAC -3'
(R):5'- TGCTCAAGCACACTCTGATG -3'

Posted On

2013-07-30