Mutagenetix > Incidental Mutation

Incidental Mutation 'R0669:Nrros'

62162

Institutional Source

Beutler Lab

Gene Symbol

Nrros

Ensembl Gene

ENSMUSG00000052384

Gene Name

negative regulator of reactive oxygen species

Synonyms

E430025L02Rik, Lrrc33

MMRRC Submission

038854-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R0669 (G1)

Quality Score

131

Status

Validated

Chromosome

Chromosomal Location

31961603-31984412 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 31962241 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Aspartic acid to Glycine at position 556 (D556G)

Ref Sequence

ENSEMBL: ENSMUSP00000155965 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000099991] [ENSMUST00000115163] [ENSMUST00000115165] [ENSMUST00000126869] [ENSMUST00000130410] [ENSMUST00000143682] [ENSMUST00000231836] [ENSMUST00000144345]

AlphaFold

Q8BMT4

Predicted Effect

probably damaging
Transcript: ENSMUST00000099991
AA Change: D564G

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000097571
Gene: ENSMUSG00000052384
AA Change: D564G

Domain	Start	End	E-Value	Type
signal peptide	1	19	N/A	INTRINSIC
LRRNT	31	61	1.05e1	SMART
LRR	80	103	1.01e2	SMART
LRR	131	155	5.27e1	SMART
LRR	156	179	4.05e-1	SMART
LRR_TYP	180	203	7.67e-2	SMART
LRR	204	227	1.49e1	SMART
LRR_TYP	327	350	1.67e-2	SMART
LRR	376	400	3.27e1	SMART
LRR_TYP	401	424	1.79e-2	SMART
LRR	461	484	1.45e1	SMART
LRR	535	556	1.76e1	SMART
LRR	559	580	3.36e1	SMART
transmembrane domain	653	675	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000115163
AA Change: D592G

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000110817
Gene: ENSMUSG00000052384
AA Change: D592G

Domain	Start	End	E-Value	Type
LRRNT	59	89	1.05e1	SMART
LRR	108	131	1.01e2	SMART
LRR	159	183	5.27e1	SMART
LRR	184	207	4.05e-1	SMART
LRR_TYP	208	231	7.67e-2	SMART
LRR	232	255	1.49e1	SMART
LRR_TYP	355	378	1.67e-2	SMART
LRR	404	428	3.27e1	SMART
LRR_TYP	429	452	1.79e-2	SMART
LRR	489	512	1.45e1	SMART
LRR	563	584	1.76e1	SMART
LRR	587	608	3.36e1	SMART
transmembrane domain	681	703	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000115165
AA Change: D540G

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000110819
Gene: ENSMUSG00000052384
AA Change: D540G

Domain	Start	End	E-Value	Type
Blast:LRRNT	8	37	5e-8	BLAST
LRR	56	79	1.01e2	SMART
LRR	107	131	5.27e1	SMART
LRR	132	155	4.05e-1	SMART
LRR_TYP	156	179	7.67e-2	SMART
LRR	180	203	1.49e1	SMART
LRR_TYP	303	326	1.67e-2	SMART
LRR	352	376	3.27e1	SMART
LRR_TYP	377	400	1.79e-2	SMART
LRR	437	460	1.45e1	SMART
LRR	511	532	1.76e1	SMART
LRR	535	556	3.36e1	SMART
transmembrane domain	629	651	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000126869
AA Change: D564G

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000116388
Gene: ENSMUSG00000052384
AA Change: D564G

Domain	Start	End	E-Value	Type
signal peptide	1	19	N/A	INTRINSIC
LRRNT	31	61	1.05e1	SMART
LRR	80	103	1.01e2	SMART
LRR	131	155	5.27e1	SMART
LRR	156	179	4.05e-1	SMART
LRR_TYP	180	203	7.67e-2	SMART
LRR	204	227	1.49e1	SMART
LRR_TYP	327	350	1.67e-2	SMART
LRR	376	400	3.27e1	SMART
LRR_TYP	401	424	1.79e-2	SMART
LRR	461	484	1.45e1	SMART
LRR	535	556	1.76e1	SMART
LRR	559	580	3.36e1	SMART
transmembrane domain	653	675	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000127287

Predicted Effect

probably benign
Transcript: ENSMUST00000130410

SMART Domains

Protein: ENSMUSP00000122290
Gene: ENSMUSG00000052384

Domain	Start	End	E-Value	Type
signal peptide	1	19	N/A	INTRINSIC
LRRNT	31	61	1.05e1	SMART
Pfam:LRR_7	81	97	1.9e-2	PFAM
Pfam:LRR_7	105	121	6.8e-2	PFAM
Pfam:LRR_7	133	144	2e-1	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000136809

Predicted Effect

probably damaging
Transcript: ENSMUST00000143682
AA Change: D564G

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000119349
Gene: ENSMUSG00000052384
AA Change: D564G

Domain	Start	End	E-Value	Type
signal peptide	1	19	N/A	INTRINSIC
LRRNT	31	61	1.05e1	SMART
LRR	80	103	1.01e2	SMART
LRR	131	155	5.27e1	SMART
LRR	156	179	4.05e-1	SMART
LRR_TYP	180	203	7.67e-2	SMART
LRR	204	227	1.49e1	SMART
LRR_TYP	327	350	1.67e-2	SMART
LRR	376	400	3.27e1	SMART
LRR_TYP	401	424	1.79e-2	SMART
LRR	461	484	1.45e1	SMART
LRR	535	556	1.76e1	SMART
LRR	559	580	3.36e1	SMART
transmembrane domain	653	675	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000150250

Predicted Effect

probably damaging
Transcript: ENSMUST00000231836
AA Change: D556G

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

Predicted Effect

probably benign
Transcript: ENSMUST00000144345

SMART Domains

Protein: ENSMUSP00000121492
Gene: ENSMUSG00000052384

Domain	Start	End	E-Value	Type
signal peptide	1	19	N/A	INTRINSIC
LRRNT	31	61	1.05e1	SMART

Meta Mutation Damage Score

0.5910

Coding Region Coverage

1x: 99.4%
3x: 98.9%
10x: 97.7%
20x: 95.8%

Validation Efficiency

99% (80/81)

MGI Phenotype

PHENOTYPE: Homozygous knockout leads to increased oxidative stress and susceptibility to experimental autoimmune encephalomyelitis, but to reduced susceptibility to bacterial infections. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 73 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4933427D14Rik	T	A	11: 72,089,671 (GRCm39)	H71L	possibly damaging	Het
Abcb11	C	A	2: 69,159,662 (GRCm39)	V10L	probably benign	Het
Abcb9	T	C	5: 124,200,950 (GRCm39)	T689A	probably damaging	Het
Adam22	A	G	5: 8,193,036 (GRCm39)		probably benign	Het
Adamts5	A	G	16: 85,696,614 (GRCm39)	I181T	probably benign	Het
Adamts9	A	T	6: 92,857,938 (GRCm39)	V231D	probably damaging	Het
Angptl6	C	T	9: 20,787,823 (GRCm39)	V197M	probably damaging	Het
Atp6v1c1	T	C	15: 38,677,772 (GRCm39)	V99A	probably benign	Het
Cacna2d3	A	G	14: 29,189,906 (GRCm39)	V110A	probably benign	Het
Ccdc32	A	G	2: 118,849,648 (GRCm39)		probably benign	Het
Cela3b	T	C	4: 137,155,841 (GRCm39)	H22R	probably benign	Het
Cep44	T	C	8: 56,994,008 (GRCm39)	T190A	possibly damaging	Het
Chsy1	T	C	7: 65,821,435 (GRCm39)	C557R	probably damaging	Het
Cntd1	A	G	11: 101,178,324 (GRCm39)	T308A	probably damaging	Het
Cutal	T	C	2: 34,775,878 (GRCm39)		probably benign	Het
Dipk1b	A	G	2: 26,524,878 (GRCm39)	T93A	probably benign	Het
Dna2	A	G	10: 62,792,768 (GRCm39)	D261G	probably damaging	Het
Dnhd1	T	A	7: 105,342,911 (GRCm39)	S1418R	probably benign	Het
Dnpep	A	G	1: 75,288,422 (GRCm39)		probably benign	Het
Dock7	A	T	4: 98,875,716 (GRCm39)	Y1075N	probably benign	Het
Eif2s1	C	T	12: 78,928,012 (GRCm39)		probably benign	Het
Ess2	T	C	16: 17,725,419 (GRCm39)	Y221C	probably damaging	Het
Fer1l6	T	C	15: 58,425,573 (GRCm39)		probably null	Het
Gm5592	T	C	7: 40,805,254 (GRCm39)		probably benign	Het
Ipp	A	G	4: 116,395,073 (GRCm39)	Y536C	probably damaging	Het
Krt72	T	C	15: 101,686,740 (GRCm39)	E402G	probably damaging	Het
Lamb3	T	C	1: 193,014,638 (GRCm39)	L599P	probably damaging	Het
Lingo2	T	A	4: 35,709,120 (GRCm39)	T287S	probably benign	Het
Lipo3	T	A	19: 33,537,025 (GRCm39)	T232S	probably benign	Het
Lrrc63	G	A	14: 75,363,550 (GRCm39)	H194Y	probably benign	Het
Map3k13	A	G	16: 21,725,274 (GRCm39)	T416A	probably benign	Het
Mcm3	A	T	1: 20,875,153 (GRCm39)		probably null	Het
Mms22l	T	C	4: 24,517,223 (GRCm39)	V258A	probably benign	Het
Mrpl46	A	T	7: 78,432,631 (GRCm39)	L49*	probably null	Het
Mrs2	T	C	13: 25,177,742 (GRCm39)	T369A	possibly damaging	Het
Mtrf1	T	A	14: 79,656,708 (GRCm39)	Y403*	probably null	Het
Muc20	G	A	16: 32,614,850 (GRCm39)	P176S	unknown	Het
Mup21	C	T	4: 62,068,964 (GRCm39)	C9Y	unknown	Het
Mup-ps21	A	T	4: 61,949,007 (GRCm39)		noncoding transcript	Het
Mybph	T	G	1: 134,125,081 (GRCm39)		probably null	Het
Mypn	A	G	10: 62,970,702 (GRCm39)		probably benign	Het
Nav2	T	C	7: 49,058,431 (GRCm39)	S124P	probably damaging	Het
Numa1	A	C	7: 101,648,884 (GRCm39)	I872L	probably benign	Het
Or14c40	A	G	7: 86,313,544 (GRCm39)	I225V	possibly damaging	Het
Or2a7	C	T	6: 43,150,938 (GRCm39)	T6I	probably benign	Het
Or2h2c	G	C	17: 37,422,347 (GRCm39)	L176V	probably benign	Het
Or4c11	A	T	2: 88,695,272 (GRCm39)	M108L	probably benign	Het
Or5p52	T	A	7: 107,502,446 (GRCm39)	I174K	probably damaging	Het
Pcdhb9	A	T	18: 37,535,308 (GRCm39)	N434I	probably damaging	Het
Pigq	A	T	17: 26,155,736 (GRCm39)		probably null	Het
Plxna2	T	G	1: 194,471,145 (GRCm39)	V972G	probably damaging	Het
Psma3	G	A	12: 71,035,269 (GRCm39)		probably benign	Het
Ptpn13	A	G	5: 103,703,975 (GRCm39)	T1336A	probably benign	Het
Rdh7	T	C	10: 127,720,598 (GRCm39)	D258G	probably benign	Het
Serpinb6c	A	G	13: 34,083,252 (GRCm39)	I54T	probably damaging	Het
Sh2d7	A	T	9: 54,448,633 (GRCm39)	Y218F	probably benign	Het
Shfl	AGAGGAGGAGGAGGAGGAGGAGGAGGA	AGAGGAGGAGGAGGAGGAGGAGGA	9: 20,785,013 (GRCm39)		probably benign	Het
Slc12a8	A	T	16: 33,371,274 (GRCm39)	I137F	possibly damaging	Het
Smarca2	T	C	19: 26,683,600 (GRCm39)	V1153A	possibly damaging	Het
Smc6	A	T	12: 11,339,165 (GRCm39)	I334L	probably benign	Het
Sorcs1	A	G	19: 50,230,380 (GRCm39)		probably benign	Het
Taar8c	A	G	10: 23,977,401 (GRCm39)	L137P	probably damaging	Het
Tcam1	A	T	11: 106,176,252 (GRCm39)	D326V	possibly damaging	Het
Telo2	A	T	17: 25,324,797 (GRCm39)	V461D	probably benign	Het
Tmprss7	A	T	16: 45,498,325 (GRCm39)	C351*	probably null	Het
Trim66	T	A	7: 109,054,199 (GRCm39)		probably benign	Het
Ube2d1	G	T	10: 71,097,940 (GRCm39)	H32N	probably benign	Het
Ubp1	T	C	9: 113,793,736 (GRCm39)		probably benign	Het
Vma21	C	T	X: 70,863,763 (GRCm39)	T81M	probably damaging	Het
Vmn1r113	T	C	7: 20,521,345 (GRCm39)	S46P	probably benign	Het
Wars1	T	C	12: 108,831,944 (GRCm39)	S374G	probably benign	Het
Wbp1	T	C	6: 83,096,326 (GRCm39)	D277G	possibly damaging	Het
Zfp939	C	A	7: 39,123,209 (GRCm39)		noncoding transcript	Het

Other mutations in Nrros

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00920:Nrros	APN	16	31,966,438 (GRCm39)	missense	probably benign	0.06
IGL01097:Nrros	APN	16	31,963,003 (GRCm39)	missense	possibly damaging	0.71
IGL02065:Nrros	APN	16	31,963,492 (GRCm39)	missense	possibly damaging	0.84
IGL03372:Nrros	APN	16	31,963,588 (GRCm39)	missense	probably damaging	1.00
R0615:Nrros	UTSW	16	31,962,903 (GRCm39)	missense	probably damaging	1.00
R0840:Nrros	UTSW	16	31,962,241 (GRCm39)	missense	probably damaging	1.00
R1398:Nrros	UTSW	16	31,961,962 (GRCm39)	missense	probably damaging	0.98
R1796:Nrros	UTSW	16	31,962,329 (GRCm39)	missense	probably damaging	1.00
R2031:Nrros	UTSW	16	31,962,975 (GRCm39)	nonsense	probably null
R2033:Nrros	UTSW	16	31,962,975 (GRCm39)	nonsense	probably null
R2034:Nrros	UTSW	16	31,962,975 (GRCm39)	nonsense	probably null
R2087:Nrros	UTSW	16	31,962,975 (GRCm39)	nonsense	probably null
R2089:Nrros	UTSW	16	31,962,975 (GRCm39)	nonsense	probably null
R2090:Nrros	UTSW	16	31,962,975 (GRCm39)	nonsense	probably null
R2091:Nrros	UTSW	16	31,962,975 (GRCm39)	nonsense	probably null
R2091:Nrros	UTSW	16	31,962,975 (GRCm39)	nonsense	probably null
R2151:Nrros	UTSW	16	31,962,076 (GRCm39)	missense	probably benign	0.11
R2438:Nrros	UTSW	16	31,963,117 (GRCm39)	missense	probably benign	0.25
R2438:Nrros	UTSW	16	31,962,929 (GRCm39)	splice site	probably null
R5474:Nrros	UTSW	16	31,963,170 (GRCm39)	missense	probably benign	0.00
R5527:Nrros	UTSW	16	31,963,288 (GRCm39)	missense	probably damaging	1.00
R5629:Nrros	UTSW	16	31,963,223 (GRCm39)	missense	probably damaging	1.00
R5888:Nrros	UTSW	16	31,961,905 (GRCm39)	missense	probably benign	0.15
R5939:Nrros	UTSW	16	31,962,272 (GRCm39)	missense	probably benign	0.01
R5982:Nrros	UTSW	16	31,963,411 (GRCm39)	missense	probably damaging	0.96
R6869:Nrros	UTSW	16	31,963,249 (GRCm39)	missense	probably damaging	1.00
R6912:Nrros	UTSW	16	31,981,057 (GRCm39)	missense	probably null	0.01
R7010:Nrros	UTSW	16	31,962,398 (GRCm39)	missense	probably damaging	0.96
R7469:Nrros	UTSW	16	31,963,030 (GRCm39)	missense	probably benign	0.14
R7673:Nrros	UTSW	16	31,981,099 (GRCm39)	missense	unknown
R7770:Nrros	UTSW	16	31,962,346 (GRCm39)	missense	probably benign	0.01
R7948:Nrros	UTSW	16	31,981,076 (GRCm39)	missense	unknown
R8375:Nrros	UTSW	16	31,966,456 (GRCm39)	missense	probably damaging	1.00
R8702:Nrros	UTSW	16	31,966,589 (GRCm39)	intron	probably benign
R9740:Nrros	UTSW	16	31,963,667 (GRCm39)	missense	possibly damaging	0.95
X0022:Nrros	UTSW	16	31,961,858 (GRCm39)	missense	possibly damaging	0.71

Predicted Primers

PCR Primer
(F):5'- AAGAGACCAGTGTCCACCTGTTCC -3'
(R):5'- AGGCATTACAAGACTGCCCGTTC -3'

Sequencing Primer
(F):5'- ACTTACAGCCCTGAGGCAG -3'
(R):5'- TTCTGAATGGGAGCATCAGCC -3'

Posted On

2013-07-30