Mutagenetix > Incidental Mutation

Incidental Mutation 'R0653:Or8g4'

62382

Institutional Source

Beutler Lab

Gene Symbol

Or8g4

Ensembl Gene

ENSMUSG00000055820

Gene Name

olfactory receptor family 8 subfamily G member 4

Synonyms

Olfr967, GA_x6K02T2PVTD-33447884-33448816, MOR171-30P

MMRRC Submission

038838-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.084) question?

Stock #

R0653 (G1)

Quality Score

131

Status

Not validated

Chromosome

Chromosomal Location

39661684-39662616 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 39661934 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Asparagine to Serine at position 84 (N84S)

Ref Sequence

ENSEMBL: ENSMUSP00000150183 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000069561] [ENSMUST00000213358]

AlphaFold

Q7TRA6

Predicted Effect

probably benign
Transcript: ENSMUST00000069561
AA Change: N84S

PolyPhen 2 Score 0.264 (Sensitivity: 0.91; Specificity: 0.88)

SMART Domains

Protein: ENSMUSP00000064201
Gene: ENSMUSG00000055820
AA Change: N84S

Domain	Start	End	E-Value	Type
Pfam:7tm_4	31	307	5.6e-50	PFAM
Pfam:7tm_1	41	290	6.8e-22	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000213358
AA Change: N84S

PolyPhen 2 Score 0.264 (Sensitivity: 0.91; Specificity: 0.88)

Coding Region Coverage

1x: 99.4%
3x: 98.9%
10x: 97.6%
20x: 95.4%

Validation Efficiency

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 55 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4933427D14Rik	T	A	11: 72,066,371 (GRCm39)	R612*	probably null	Het
Adgra1	A	G	7: 139,456,063 (GRCm39)	T564A	probably damaging	Het
Akr1c18	A	T	13: 4,195,307 (GRCm39)	D50E	probably damaging	Het
Atg9a	C	A	1: 75,166,972 (GRCm39)	L26F	probably damaging	Het
Atp11b	A	G	3: 35,893,343 (GRCm39)	T899A	probably damaging	Het
Atxn2	A	G	5: 121,910,841 (GRCm39)	E358G	probably damaging	Het
Bmp3	A	G	5: 99,019,970 (GRCm39)	Y131C	probably damaging	Het
Brip1	T	C	11: 86,043,484 (GRCm39)	E360G	possibly damaging	Het
Casd1	A	T	6: 4,608,075 (GRCm39)	I76L	probably benign	Het
Casq2	G	A	3: 102,020,482 (GRCm39)		probably null	Het
Ccdc185	T	A	1: 182,575,129 (GRCm39)	Q520L	possibly damaging	Het
Cenpf	T	C	1: 189,392,183 (GRCm39)	K550E	probably damaging	Het
Ciz1	T	C	2: 32,262,418 (GRCm39)	S521P	probably damaging	Het
Clstn2	A	T	9: 97,340,257 (GRCm39)	V705E	probably damaging	Het
Dagla	A	G	19: 10,225,789 (GRCm39)	Y792H	probably damaging	Het
Dgke	A	T	11: 88,950,995 (GRCm39)	C73S	probably benign	Het
Egflam	A	G	15: 7,279,509 (GRCm39)		probably null	Het
Farp1	T	C	14: 121,471,258 (GRCm39)		probably null	Het
Fos	A	G	12: 85,522,790 (GRCm39)	E234G	probably benign	Het
Gtf3c5	A	T	2: 28,468,008 (GRCm39)	M151K	probably benign	Het
Hgs	G	A	11: 120,359,904 (GRCm39)	R36H	probably damaging	Het
Lats2	G	A	14: 57,937,653 (GRCm39)	Q279*	probably null	Het
Lysmd4	T	A	7: 66,875,788 (GRCm39)	D150E	probably benign	Het
Mex3b	A	G	7: 82,518,242 (GRCm39)	K186E	probably damaging	Het
Myo9a	A	C	9: 59,832,274 (GRCm39)	Q2601P	probably damaging	Het
Nckap5l	C	A	15: 99,321,127 (GRCm39)	V1218F	probably damaging	Het
Nr5a2	A	G	1: 136,876,543 (GRCm39)	V40A	probably benign	Het
Obscn	C	A	11: 58,898,534 (GRCm39)		probably benign	Het
Or2y1d	C	A	11: 49,322,078 (GRCm39)	Y258*	probably null	Het
Or4c114	A	G	2: 88,904,808 (GRCm39)	I209T	possibly damaging	Het
Pclo	T	A	5: 14,732,269 (GRCm39)		probably benign	Het
Reln	T	C	5: 22,118,228 (GRCm39)	I2939V	probably benign	Het
Rtn4	A	T	11: 29,657,256 (GRCm39)	K470I	probably damaging	Het
Sbno1	A	G	5: 124,524,955 (GRCm39)	I1050T	possibly damaging	Het
Scaf11	G	T	15: 96,316,522 (GRCm39)	S17*	probably null	Het
Scn9a	A	T	2: 66,363,721 (GRCm39)	N841K	probably damaging	Het
Slc35e3	C	A	10: 117,576,711 (GRCm39)	E207*	probably null	Het
Slc6a20b	A	G	9: 123,426,377 (GRCm39)	F503L	probably damaging	Het
Spag16	G	T	1: 69,909,504 (GRCm39)	K200N	probably damaging	Het
Supt6	G	T	11: 78,116,841 (GRCm39)	Q604K	probably benign	Het
Taok1	A	G	11: 77,469,550 (GRCm39)		probably null	Het
Tjp1	A	T	7: 64,964,503 (GRCm39)	H889Q	probably damaging	Het
Tmed6	A	T	8: 107,792,283 (GRCm39)		probably null	Het
Tsen54	A	T	11: 115,705,887 (GRCm39)	E68V	probably damaging	Het
Ttn	A	G	2: 76,559,878 (GRCm39)	S21181P	probably damaging	Het
Ube3d	A	T	9: 86,334,043 (GRCm39)	M33K	possibly damaging	Het
Umodl1	C	A	17: 31,203,002 (GRCm39)	Q452K	probably benign	Het
Wif1	G	T	10: 120,935,704 (GRCm39)	A354S	probably benign	Het
Wnk2	A	G	13: 49,210,492 (GRCm39)	F1788L	possibly damaging	Het
Zfhx3	A	T	8: 109,673,440 (GRCm39)	I1497F	possibly damaging	Het
Zfp217	C	T	2: 169,957,382 (GRCm39)	A539T	probably benign	Het
Zfp30	G	A	7: 29,492,178 (GRCm39)	R225Q	probably damaging	Het
Zfp87	T	C	13: 74,520,190 (GRCm39)	E296G	probably damaging	Het
Zfp874b	A	G	13: 67,623,052 (GRCm39)	F86S	possibly damaging	Het
Zfyve19	A	G	2: 119,041,696 (GRCm39)	S88G	probably benign	Het

Other mutations in Or8g4

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02418:Or8g4	APN	9	39,661,787 (GRCm39)	missense	probably damaging	0.99
IGL02900:Or8g4	APN	9	39,661,901 (GRCm39)	missense	probably benign	0.01
R0099:Or8g4	UTSW	9	39,661,957 (GRCm39)	missense	possibly damaging	0.95
R0586:Or8g4	UTSW	9	39,662,414 (GRCm39)	missense	probably damaging	0.98
R0839:Or8g4	UTSW	9	39,661,687 (GRCm39)	missense	probably benign
R1701:Or8g4	UTSW	9	39,662,365 (GRCm39)	missense	probably damaging	1.00
R1744:Or8g4	UTSW	9	39,661,711 (GRCm39)	missense	probably benign	0.33
R1902:Or8g4	UTSW	9	39,662,102 (GRCm39)	missense	probably benign	0.01
R4696:Or8g4	UTSW	9	39,662,024 (GRCm39)	missense	probably damaging	0.98
R5252:Or8g4	UTSW	9	39,661,784 (GRCm39)	missense	probably damaging	0.98
R5660:Or8g4	UTSW	9	39,662,063 (GRCm39)	missense	probably damaging	1.00
R6272:Or8g4	UTSW	9	39,661,816 (GRCm39)	missense	probably benign	0.39
R6976:Or8g4	UTSW	9	39,662,540 (GRCm39)	missense	probably damaging	1.00
R7078:Or8g4	UTSW	9	39,661,787 (GRCm39)	missense	possibly damaging	0.92
R7167:Or8g4	UTSW	9	39,661,865 (GRCm39)	missense	probably damaging	0.96
R7701:Or8g4	UTSW	9	39,662,597 (GRCm39)	missense	probably benign
R7980:Or8g4	UTSW	9	39,662,417 (GRCm39)	missense	probably damaging	0.99
R8026:Or8g4	UTSW	9	39,662,092 (GRCm39)	missense	possibly damaging	0.80
R8701:Or8g4	UTSW	9	39,662,210 (GRCm39)	missense	probably damaging	1.00
R8790:Or8g4	UTSW	9	39,662,204 (GRCm39)	missense	probably damaging	1.00
R8822:Or8g4	UTSW	9	39,661,700 (GRCm39)	missense	probably benign	0.01
R8825:Or8g4	UTSW	9	39,661,994 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- GGAACTGCTTGACCTTGGTTTCACAC -3'
(R):5'- TCATCCAGGTGCAGACTTGATAGGAC -3'

Sequencing Primer
(F):5'- TCCTTGCTGGATTAACAAGCAC -3'
(R):5'- TGCAGACTTGATAGGACATCATAGC -3'

Posted On

2013-07-30