Incidental Mutation 'R0653:Wif1'
ID 62389
Institutional Source Beutler Lab
Gene Symbol Wif1
Ensembl Gene ENSMUSG00000020218
Gene Name Wnt inhibitory factor 1
Synonyms WIF-1
MMRRC Submission 038838-MU
Accession Numbers
Essential gene? Non essential (E-score: 0.000) question?
Stock # R0653 (G1)
Quality Score 82
Status Not validated
Chromosome 10
Chromosomal Location 120869909-120936547 bp(+) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) G to T at 120935704 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Alanine to Serine at position 354 (A354S)
Ref Sequence ENSEMBL: ENSMUSP00000020439 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000020439] [ENSMUST00000175867]
AlphaFold Q9WUA1
Predicted Effect probably benign
Transcript: ENSMUST00000020439
AA Change: A354S

PolyPhen 2 Score 0.010 (Sensitivity: 0.96; Specificity: 0.77)
SMART Domains Protein: ENSMUSP00000020439
Gene: ENSMUSG00000020218
AA Change: A354S

DomainStartEndE-ValueType
signal peptide 1 28 N/A INTRINSIC
WIF 35 179 8.47e-90 SMART
EGF 181 210 3.88e-3 SMART
EGF 213 242 4.46e-3 SMART
EGF 245 274 4.7e-2 SMART
EGF 277 306 1.69e-3 SMART
EGF 309 338 7.95e0 SMART
Predicted Effect noncoding transcript
Transcript: ENSMUST00000145691
Predicted Effect probably benign
Transcript: ENSMUST00000175867
AA Change: A340S

PolyPhen 2 Score 0.002 (Sensitivity: 0.99; Specificity: 0.30)
SMART Domains Protein: ENSMUSP00000135486
Gene: ENSMUSG00000020218
AA Change: A340S

DomainStartEndE-ValueType
signal peptide 1 28 N/A INTRINSIC
WIF 35 179 8.47e-90 SMART
EGF 181 210 3.88e-3 SMART
EGF 213 242 4.46e-3 SMART
EGF 245 274 4.7e-2 SMART
EGF 295 324 7.95e0 SMART
Coding Region Coverage
  • 1x: 99.4%
  • 3x: 98.9%
  • 10x: 97.6%
  • 20x: 95.4%
Validation Efficiency
MGI Phenotype FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene functions to inhibit WNT proteins, which are extracellular signaling molecules that play a role in embryonic development. This protein contains a WNT inhibitory factor (WIF) domain and five epidermal growth factor (EGF)-like domains, and is thought to be involved in mesoderm segmentation. This gene functions as a tumor suppressor gene, and has been found to be epigenetically silenced in various cancers. [provided by RefSeq, Jun 2010]
PHENOTYPE: Homozygous null mice are viable and fertile but display increased susceptibility to spontaneous and induced osteosarcomas. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 55 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
4933427D14Rik T A 11: 72,066,371 (GRCm39) R612* probably null Het
Adgra1 A G 7: 139,456,063 (GRCm39) T564A probably damaging Het
Akr1c18 A T 13: 4,195,307 (GRCm39) D50E probably damaging Het
Atg9a C A 1: 75,166,972 (GRCm39) L26F probably damaging Het
Atp11b A G 3: 35,893,343 (GRCm39) T899A probably damaging Het
Atxn2 A G 5: 121,910,841 (GRCm39) E358G probably damaging Het
Bmp3 A G 5: 99,019,970 (GRCm39) Y131C probably damaging Het
Brip1 T C 11: 86,043,484 (GRCm39) E360G possibly damaging Het
Casd1 A T 6: 4,608,075 (GRCm39) I76L probably benign Het
Casq2 G A 3: 102,020,482 (GRCm39) probably null Het
Ccdc185 T A 1: 182,575,129 (GRCm39) Q520L possibly damaging Het
Cenpf T C 1: 189,392,183 (GRCm39) K550E probably damaging Het
Ciz1 T C 2: 32,262,418 (GRCm39) S521P probably damaging Het
Clstn2 A T 9: 97,340,257 (GRCm39) V705E probably damaging Het
Dagla A G 19: 10,225,789 (GRCm39) Y792H probably damaging Het
Dgke A T 11: 88,950,995 (GRCm39) C73S probably benign Het
Egflam A G 15: 7,279,509 (GRCm39) probably null Het
Farp1 T C 14: 121,471,258 (GRCm39) probably null Het
Fos A G 12: 85,522,790 (GRCm39) E234G probably benign Het
Gtf3c5 A T 2: 28,468,008 (GRCm39) M151K probably benign Het
Hgs G A 11: 120,359,904 (GRCm39) R36H probably damaging Het
Lats2 G A 14: 57,937,653 (GRCm39) Q279* probably null Het
Lysmd4 T A 7: 66,875,788 (GRCm39) D150E probably benign Het
Mex3b A G 7: 82,518,242 (GRCm39) K186E probably damaging Het
Myo9a A C 9: 59,832,274 (GRCm39) Q2601P probably damaging Het
Nckap5l C A 15: 99,321,127 (GRCm39) V1218F probably damaging Het
Nr5a2 A G 1: 136,876,543 (GRCm39) V40A probably benign Het
Obscn C A 11: 58,898,534 (GRCm39) probably benign Het
Or2y1d C A 11: 49,322,078 (GRCm39) Y258* probably null Het
Or4c114 A G 2: 88,904,808 (GRCm39) I209T possibly damaging Het
Or8g4 A G 9: 39,661,934 (GRCm39) N84S probably benign Het
Pclo T A 5: 14,732,269 (GRCm39) probably benign Het
Reln T C 5: 22,118,228 (GRCm39) I2939V probably benign Het
Rtn4 A T 11: 29,657,256 (GRCm39) K470I probably damaging Het
Sbno1 A G 5: 124,524,955 (GRCm39) I1050T possibly damaging Het
Scaf11 G T 15: 96,316,522 (GRCm39) S17* probably null Het
Scn9a A T 2: 66,363,721 (GRCm39) N841K probably damaging Het
Slc35e3 C A 10: 117,576,711 (GRCm39) E207* probably null Het
Slc6a20b A G 9: 123,426,377 (GRCm39) F503L probably damaging Het
Spag16 G T 1: 69,909,504 (GRCm39) K200N probably damaging Het
Supt6 G T 11: 78,116,841 (GRCm39) Q604K probably benign Het
Taok1 A G 11: 77,469,550 (GRCm39) probably null Het
Tjp1 A T 7: 64,964,503 (GRCm39) H889Q probably damaging Het
Tmed6 A T 8: 107,792,283 (GRCm39) probably null Het
Tsen54 A T 11: 115,705,887 (GRCm39) E68V probably damaging Het
Ttn A G 2: 76,559,878 (GRCm39) S21181P probably damaging Het
Ube3d A T 9: 86,334,043 (GRCm39) M33K possibly damaging Het
Umodl1 C A 17: 31,203,002 (GRCm39) Q452K probably benign Het
Wnk2 A G 13: 49,210,492 (GRCm39) F1788L possibly damaging Het
Zfhx3 A T 8: 109,673,440 (GRCm39) I1497F possibly damaging Het
Zfp217 C T 2: 169,957,382 (GRCm39) A539T probably benign Het
Zfp30 G A 7: 29,492,178 (GRCm39) R225Q probably damaging Het
Zfp87 T C 13: 74,520,190 (GRCm39) E296G probably damaging Het
Zfp874b A G 13: 67,623,052 (GRCm39) F86S possibly damaging Het
Zfyve19 A G 2: 119,041,696 (GRCm39) S88G probably benign Het
Other mutations in Wif1
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01384:Wif1 APN 10 120,920,855 (GRCm39) missense possibly damaging 0.55
IGL01707:Wif1 APN 10 120,919,890 (GRCm39) critical splice donor site probably null
IGL01932:Wif1 APN 10 120,931,940 (GRCm39) missense probably damaging 1.00
IGL02183:Wif1 APN 10 120,911,181 (GRCm39) missense probably damaging 1.00
R1858:Wif1 UTSW 10 120,919,788 (GRCm39) splice site probably null
R1869:Wif1 UTSW 10 120,920,824 (GRCm39) missense probably benign 0.00
R1871:Wif1 UTSW 10 120,920,824 (GRCm39) missense probably benign 0.00
R4056:Wif1 UTSW 10 120,918,099 (GRCm39) missense probably benign 0.04
R4057:Wif1 UTSW 10 120,918,099 (GRCm39) missense probably benign 0.04
R5056:Wif1 UTSW 10 120,935,684 (GRCm39) missense probably benign 0.00
R6242:Wif1 UTSW 10 120,870,366 (GRCm39) missense possibly damaging 0.82
R6504:Wif1 UTSW 10 120,870,996 (GRCm39) missense probably damaging 0.96
R7220:Wif1 UTSW 10 120,926,019 (GRCm39) missense possibly damaging 0.76
R7365:Wif1 UTSW 10 120,919,814 (GRCm39) missense possibly damaging 0.94
R7456:Wif1 UTSW 10 120,932,554 (GRCm39) missense probably benign
R7707:Wif1 UTSW 10 120,919,864 (GRCm39) missense probably damaging 0.97
R8283:Wif1 UTSW 10 120,931,952 (GRCm39) missense probably benign
R8817:Wif1 UTSW 10 120,932,621 (GRCm39) missense possibly damaging 0.95
R8940:Wif1 UTSW 10 120,935,684 (GRCm39) missense probably benign 0.11
R8959:Wif1 UTSW 10 120,931,957 (GRCm39) missense probably damaging 0.99
R9628:Wif1 UTSW 10 120,932,549 (GRCm39) missense possibly damaging 0.94
Z1176:Wif1 UTSW 10 120,932,561 (GRCm39) missense probably damaging 1.00
Predicted Primers PCR Primer
(F):5'- AGACCTTGAACCTGTCACCTGCTG -3'
(R):5'- CGCTGCTATTGGCTTTATCCGAAAC -3'

Sequencing Primer
(F):5'- ACCTGCTGTTATTCAGGTAACTC -3'
(R):5'- TAAGATCTGTCCCAAAAGGACTG -3'
Posted On 2013-07-30