Mutagenetix > Incidental Mutation

Incidental Mutation 'R0051:Zfp775'

64287

Institutional Source

Beutler Lab

Gene Symbol

Zfp775

Ensembl Gene

ENSMUSG00000007216

Gene Name

zinc finger protein 775

Synonyms

C130032F08Rik

MMRRC Submission

038345-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.117) question?

Stock #

R0051 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

48578937-48598482 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 48597706 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Alanine at position 527 (T527A)

Ref Sequence

ENSEMBL: ENSMUSP00000145192 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000061720] [ENSMUST00000204042] [ENSMUST00000204095] [ENSMUST00000204121]

AlphaFold

Q8BI73

Predicted Effect

probably benign
Transcript: ENSMUST00000061720
AA Change: T527A

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000056290
Gene: ENSMUSG00000007216
AA Change: T527A

Domain	Start	End	E-Value	Type
low complexity region	86	102	N/A	INTRINSIC
ZnF_C2H2	104	126	7.9e-4	SMART
ZnF_C2H2	132	154	1.69e-3	SMART
ZnF_C2H2	160	182	2.36e-2	SMART
ZnF_C2H2	188	210	4.11e-2	SMART
ZnF_C2H2	279	301	1.84e-4	SMART
ZnF_C2H2	307	329	4.94e-5	SMART
ZnF_C2H2	335	357	2.95e-3	SMART
ZnF_C2H2	364	386	1.36e-2	SMART
ZnF_C2H2	446	468	1.3e-4	SMART
ZnF_C2H2	474	496	2.12e-4	SMART
ZnF_C2H2	502	524	5.5e-3	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000203998

Predicted Effect

probably benign
Transcript: ENSMUST00000204042

SMART Domains

Protein: ENSMUSP00000145369
Gene: ENSMUSG00000007216

Domain	Start	End	E-Value	Type
low complexity region	86	102	N/A	INTRINSIC
ZnF_C2H2	104	126	3.3e-6	SMART
ZnF_C2H2	132	154	7.3e-6	SMART
ZnF_C2H2	160	182	9.9e-5	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000204095
AA Change: T527A

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000145192
Gene: ENSMUSG00000007216
AA Change: T527A

Domain	Start	End	E-Value	Type
low complexity region	86	102	N/A	INTRINSIC
ZnF_C2H2	104	126	7.9e-4	SMART
ZnF_C2H2	132	154	1.69e-3	SMART
ZnF_C2H2	160	182	2.36e-2	SMART
ZnF_C2H2	188	210	4.11e-2	SMART
ZnF_C2H2	279	301	1.84e-4	SMART
ZnF_C2H2	307	329	4.94e-5	SMART
ZnF_C2H2	335	357	2.95e-3	SMART
ZnF_C2H2	364	386	1.36e-2	SMART
ZnF_C2H2	446	468	1.3e-4	SMART
ZnF_C2H2	474	496	2.12e-4	SMART
ZnF_C2H2	502	524	5.5e-3	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000204121

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 99.2%
3x: 98.7%
10x: 97.1%
20x: 93.8%

Validation Efficiency

100% (64/64)

Allele List at MGI

Other mutations in this stock

Total: 54 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930432E11Rik	A	G	7: 29,278,526 (GRCm39)		noncoding transcript	Het
Abr	T	A	11: 76,363,328 (GRCm39)	Q163L	probably benign	Het
Ankrd11	C	A	8: 123,616,481 (GRCm39)	C2457F	probably damaging	Het
Anks3	G	C	16: 4,765,613 (GRCm39)	T163S	probably benign	Het
Cacna1d	G	A	14: 29,833,052 (GRCm39)	P908S	probably damaging	Het
Ccdc146	C	A	5: 21,521,902 (GRCm39)	R374L	possibly damaging	Het
Cdc45	G	T	16: 18,613,524 (GRCm39)	A348E	probably damaging	Het
Cfap46	A	G	7: 139,255,951 (GRCm39)	C300R	probably damaging	Het
Cimap1a	C	A	7: 140,430,134 (GRCm39)		probably benign	Het
Coq2	T	C	5: 100,811,551 (GRCm39)	N146S	probably benign	Het
Dalrd3	T	C	9: 108,449,414 (GRCm39)	V120A	possibly damaging	Het
Dcp2	T	A	18: 44,538,441 (GRCm39)		probably benign	Het
Ddx39a	A	G	8: 84,447,251 (GRCm39)	K137R	possibly damaging	Het
Diaph3	A	G	14: 87,274,890 (GRCm39)		probably null	Het
Dmbt1	G	T	7: 130,721,225 (GRCm39)	R1668L	possibly damaging	Het
Dnah7a	T	G	1: 53,560,245 (GRCm39)		probably benign	Het
Dpp7	A	G	2: 25,246,107 (GRCm39)	Y49H	possibly damaging	Het
Drd5	A	G	5: 38,477,957 (GRCm39)	S317G	probably benign	Het
Ecpas	A	G	4: 58,832,729 (GRCm39)	L877S	probably damaging	Het
Ecsit	C	T	9: 21,987,584 (GRCm39)	V152I	probably benign	Het
Eeig1	G	A	2: 32,448,065 (GRCm39)	R58Q	possibly damaging	Het
Emc1	T	A	4: 139,102,474 (GRCm39)	M923K	possibly damaging	Het
Fcrl6	A	T	1: 172,426,320 (GRCm39)	L159Q	probably benign	Het
Frrs1	T	C	3: 116,678,946 (GRCm39)		probably benign	Het
Hspd1	A	G	1: 55,121,205 (GRCm39)		probably benign	Het
Impg2	T	A	16: 56,078,411 (GRCm39)	S458T	probably damaging	Het
Klf17	T	C	4: 117,617,589 (GRCm39)	Y256C	probably damaging	Het
Lnx2	A	G	5: 146,966,163 (GRCm39)	F319L	probably damaging	Het
Mafg	G	T	11: 120,520,430 (GRCm39)	R57S	probably damaging	Het
Med13l	T	A	5: 118,880,720 (GRCm39)	W1271R	probably damaging	Het
Mrgprb1	A	G	7: 48,096,962 (GRCm39)	S24P	probably benign	Het
Mtrf1l	T	C	10: 5,763,384 (GRCm39)	E315G	probably damaging	Het
Naip1	T	C	13: 100,547,509 (GRCm39)	E1239G	probably damaging	Het
Ncaph2	T	C	15: 89,253,867 (GRCm39)	S320P	probably damaging	Het
Nek11	A	G	9: 105,095,738 (GRCm39)		probably benign	Het
Nlrp2	A	T	7: 5,325,333 (GRCm39)		probably benign	Het
Rbm26	A	C	14: 105,389,976 (GRCm39)	V216G	possibly damaging	Het
Rnf115	A	G	3: 96,692,338 (GRCm39)	D178G	probably damaging	Het
Rtel1	C	T	2: 180,992,449 (GRCm39)	Q424*	probably null	Het
Rwdd4a	A	G	8: 47,990,400 (GRCm39)		probably benign	Het
Ryr3	T	C	2: 112,699,420 (GRCm39)	D890G	probably damaging	Het
Scarb1	C	A	5: 125,358,164 (GRCm39)		probably null	Het
Serpina10	A	G	12: 103,593,156 (GRCm39)		probably benign	Het
Slc12a5	T	A	2: 164,828,583 (GRCm39)	W508R	probably damaging	Het
Slc43a2	T	C	11: 75,453,676 (GRCm39)	C225R	probably damaging	Het
Slc6a9	T	C	4: 117,722,056 (GRCm39)	F440L	probably damaging	Het
Stac3	G	A	10: 127,344,017 (GRCm39)	R305H	probably damaging	Het
Stk32b	A	G	5: 37,616,940 (GRCm39)		probably benign	Het
Syna	A	T	5: 134,588,397 (GRCm39)	L184H	probably damaging	Het
Tmprss7	T	C	16: 45,494,302 (GRCm39)	N401S	probably damaging	Het
Tut4	T	G	4: 108,384,201 (GRCm39)	S1089R	probably damaging	Het
Yeats2	T	A	16: 20,012,474 (GRCm39)	Y557*	probably null	Het
Zfp352	T	C	4: 90,112,522 (GRCm39)	S221P	probably damaging	Het
Zfp575	A	G	7: 24,285,512 (GRCm39)	V43A	probably benign	Het

Other mutations in Zfp775

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
R1694:Zfp775	UTSW	6	48,596,389 (GRCm39)	missense	possibly damaging	0.53
R4178:Zfp775	UTSW	6	48,590,187 (GRCm39)	splice site	probably null
R5992:Zfp775	UTSW	6	48,596,750 (GRCm39)	missense	probably damaging	1.00
R6536:Zfp775	UTSW	6	48,596,543 (GRCm39)	missense	probably damaging	1.00
R6924:Zfp775	UTSW	6	48,596,589 (GRCm39)	missense	probably damaging	1.00
R7200:Zfp775	UTSW	6	48,597,415 (GRCm39)	missense	possibly damaging	0.47
R7784:Zfp775	UTSW	6	48,596,183 (GRCm39)	missense	possibly damaging	0.94
R7872:Zfp775	UTSW	6	48,597,404 (GRCm39)	missense	probably benign
R8406:Zfp775	UTSW	6	48,597,637 (GRCm39)	missense	probably damaging	1.00
R8766:Zfp775	UTSW	6	48,597,113 (GRCm39)	missense	probably damaging	1.00
R8771:Zfp775	UTSW	6	48,596,906 (GRCm39)	missense	probably benign	0.34
R9068:Zfp775	UTSW	6	48,597,151 (GRCm39)	missense	probably damaging	1.00
Z1088:Zfp775	UTSW	6	48,597,622 (GRCm39)	missense	probably damaging	1.00
Z1177:Zfp775	UTSW	6	48,597,245 (GRCm39)	missense	possibly damaging	0.50

Predicted Primers

PCR Primer
(F):5'- AGTGTGGTTGTGAAGCCCCAAG -3'
(R):5'- TAGAATACCATCCTCGGGCCTCAG -3'

Sequencing Primer
(F):5'- CAGTTCATCTGCAACGAGTG -3'
(R):5'- GCCTCAGAACTTTGATGAGC -3'

Posted On

2013-08-06