Incidental Mutation 'R0034:Kif15'
ID 64592
Institutional Source Beutler Lab
Gene Symbol Kif15
Ensembl Gene ENSMUSG00000036768
Gene Name kinesin family member 15
Synonyms N-10 kinesin, 3930402I10Rik, 3110023M17Rik, HKLP2, Knsl7
MMRRC Submission 038328-MU
Accession Numbers
Essential gene? Non essential (E-score: 0.000) question?
Stock # R0034 (G1)
Quality Score 140
Status Validated
Chromosome 9
Chromosomal Location 122780146-122847798 bp(+) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) A to T at 122828350 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Asparagine to Isoleucine at position 887 (N887I)
Ref Sequence ENSEMBL: ENSMUSP00000035490 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000040717] [ENSMUST00000214652]
AlphaFold Q6P9L6
Predicted Effect possibly damaging
Transcript: ENSMUST00000040717
AA Change: N887I

PolyPhen 2 Score 0.468 (Sensitivity: 0.89; Specificity: 0.90)
SMART Domains Protein: ENSMUSP00000035490
Gene: ENSMUSG00000036768
AA Change: N887I

DomainStartEndE-ValueType
KISc 24 371 2.86e-179 SMART
Pfam:Kinesin-relat_1 463 551 6.6e-26 PFAM
coiled coil region 579 643 N/A INTRINSIC
coiled coil region 706 1037 N/A INTRINSIC
coiled coil region 1065 1133 N/A INTRINSIC
Pfam:HMMR_C 1265 1387 3.5e-12 PFAM
Predicted Effect noncoding transcript
Transcript: ENSMUST00000214183
Predicted Effect probably benign
Transcript: ENSMUST00000214652
AA Change: N659I

PolyPhen 2 Score 0.403 (Sensitivity: 0.89; Specificity: 0.89)
Meta Mutation Damage Score 0.0632 question?
Coding Region Coverage
  • 1x: 99.4%
  • 3x: 98.9%
  • 10x: 97.8%
  • 20x: 96.4%
Validation Efficiency 98% (64/65)
Allele List at MGI
Other mutations in this stock
Total: 58 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
9430038I01Rik C T 7: 136,989,321 (GRCm39) R60Q probably benign Het
Angpt4 C T 2: 151,771,311 (GRCm39) T209I probably benign Het
Ap3b1 T C 13: 94,616,393 (GRCm39) probably benign Het
Aplp1 A C 7: 30,143,867 (GRCm39) V56G probably damaging Het
Asns G A 6: 7,676,299 (GRCm39) P419L probably damaging Het
Atxn7 A T 14: 14,100,846 (GRCm38) H844L probably damaging Het
Cd14 A G 18: 36,859,288 (GRCm39) Y56H probably benign Het
Cd300lb C T 11: 114,819,225 (GRCm39) V135I probably damaging Het
Cep152 C T 2: 125,425,813 (GRCm39) A851T probably benign Het
Cfap74 C T 4: 155,545,344 (GRCm39) probably benign Het
Col28a1 T A 6: 8,175,708 (GRCm39) I47L probably benign Het
Dnaaf9 T C 2: 130,578,492 (GRCm39) H664R probably damaging Het
Eef1d T C 15: 75,774,808 (GRCm39) T200A probably benign Het
Exoc1l A G 5: 76,664,377 (GRCm39) I155M probably damaging Het
Faap100 A T 11: 120,262,973 (GRCm39) M795K probably benign Het
Gabpb1 C T 2: 126,500,454 (GRCm39) R15Q possibly damaging Het
Gata4 C A 14: 63,438,933 (GRCm39) M381I probably benign Het
Gm5114 A G 7: 39,058,282 (GRCm39) S446P possibly damaging Het
Gnb1 T A 4: 155,636,146 (GRCm39) N155K probably benign Het
Haspin G A 11: 73,029,044 (GRCm39) T15M probably damaging Het
Heatr5a A G 12: 51,971,955 (GRCm39) L745P probably damaging Het
Kcng3 T A 17: 83,895,812 (GRCm39) probably benign Het
Kif26a T C 12: 112,135,397 (GRCm39) probably benign Het
Kif9 G A 9: 110,348,679 (GRCm39) C738Y probably benign Het
Kifc2 G T 15: 76,551,300 (GRCm39) C613F probably benign Het
Klf12 A G 14: 100,224,865 (GRCm39) probably null Het
Lrp1 A T 10: 127,381,520 (GRCm39) I3826N probably benign Het
Map2k4 A G 11: 65,610,437 (GRCm39) probably benign Het
Myo7b A G 18: 32,093,913 (GRCm39) S2006P probably damaging Het
Or51m1 T C 7: 103,578,708 (GRCm39) V226A probably benign Het
Pax4 T C 6: 28,442,448 (GRCm39) T285A probably benign Het
Pcdhb5 A G 18: 37,455,137 (GRCm39) N506D probably damaging Het
Pkhd1l1 G A 15: 44,367,405 (GRCm39) G768S probably benign Het
Plb1 G T 5: 32,430,457 (GRCm39) G138V probably benign Het
Poln A C 5: 34,272,762 (GRCm39) V398G possibly damaging Het
Poteg A G 8: 27,952,105 (GRCm39) probably benign Het
Rapgef1 C A 2: 29,614,780 (GRCm39) probably benign Het
Rbm43 A T 2: 51,815,722 (GRCm39) D166E probably benign Het
Rhobtb2 T C 14: 70,026,137 (GRCm39) T602A probably benign Het
Samd3 G A 10: 26,147,398 (GRCm39) probably benign Het
Sbno2 A C 10: 79,894,174 (GRCm39) probably benign Het
Sec1 A G 7: 45,328,759 (GRCm39) V96A probably benign Het
Senp7 A C 16: 55,973,933 (GRCm39) S385R possibly damaging Het
Sgk3 T C 1: 9,955,902 (GRCm39) V301A probably damaging Het
Sgpl1 A T 10: 60,938,392 (GRCm39) M467K probably damaging Het
Slc22a26 A G 19: 7,779,618 (GRCm39) I66T probably benign Het
Stra6 G A 9: 58,058,752 (GRCm39) probably null Het
Tfrc T A 16: 32,434,214 (GRCm39) probably null Het
Tmem30b T C 12: 73,592,779 (GRCm39) Y112C probably damaging Het
Trap1 A T 16: 3,886,894 (GRCm39) probably benign Het
Trpc1 A G 9: 95,631,814 (GRCm39) S43P probably damaging Het
Tsku T C 7: 98,001,870 (GRCm39) T154A possibly damaging Het
Uroc1 T C 6: 90,322,292 (GRCm39) V272A probably damaging Het
Vmn1r69 T A 7: 10,314,738 (GRCm39) probably benign Het
Vmn2r1 T A 3: 63,997,435 (GRCm39) W364R probably damaging Het
Wnk2 G T 13: 49,221,556 (GRCm39) T377K possibly damaging Het
Zfta C A 19: 7,397,724 (GRCm39) H90Q probably damaging Het
Zscan20 T C 4: 128,479,455 (GRCm39) N1012S probably damaging Het
Other mutations in Kif15
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01459:Kif15 APN 9 122,804,820 (GRCm39) missense probably damaging 1.00
IGL01577:Kif15 APN 9 122,825,399 (GRCm39) missense probably benign 0.06
IGL01647:Kif15 APN 9 122,792,536 (GRCm39) intron probably benign
IGL01921:Kif15 APN 9 122,808,569 (GRCm39) missense probably damaging 1.00
IGL02040:Kif15 APN 9 122,846,450 (GRCm39) missense probably damaging 0.99
IGL02191:Kif15 APN 9 122,804,744 (GRCm39) missense probably damaging 1.00
IGL02218:Kif15 APN 9 122,824,892 (GRCm39) splice site probably benign
IGL02537:Kif15 APN 9 122,822,914 (GRCm39) missense probably benign 0.08
IGL02814:Kif15 APN 9 122,832,705 (GRCm39) missense possibly damaging 0.83
PIT4480001:Kif15 UTSW 9 122,840,608 (GRCm39) missense probably benign
R0458:Kif15 UTSW 9 122,838,424 (GRCm39) missense probably benign
R0526:Kif15 UTSW 9 122,826,862 (GRCm39) missense probably damaging 0.96
R0533:Kif15 UTSW 9 122,838,498 (GRCm39) unclassified probably benign
R0726:Kif15 UTSW 9 122,788,993 (GRCm39) missense probably benign 0.21
R1580:Kif15 UTSW 9 122,789,021 (GRCm39) missense probably benign 0.22
R1597:Kif15 UTSW 9 122,823,074 (GRCm39) missense probably benign 0.22
R2096:Kif15 UTSW 9 122,815,252 (GRCm39) missense probably damaging 1.00
R3125:Kif15 UTSW 9 122,817,026 (GRCm39) missense probably damaging 0.99
R3176:Kif15 UTSW 9 122,816,905 (GRCm39) splice site probably benign
R4088:Kif15 UTSW 9 122,815,254 (GRCm39) missense probably benign 0.29
R4308:Kif15 UTSW 9 122,843,047 (GRCm39) missense probably benign 0.00
R4597:Kif15 UTSW 9 122,822,914 (GRCm39) missense probably benign 0.08
R4705:Kif15 UTSW 9 122,789,058 (GRCm39) splice site probably null
R4832:Kif15 UTSW 9 122,831,191 (GRCm39) splice site probably null
R5100:Kif15 UTSW 9 122,821,059 (GRCm39) missense probably damaging 0.98
R5126:Kif15 UTSW 9 122,804,823 (GRCm39) missense probably damaging 1.00
R5180:Kif15 UTSW 9 122,828,275 (GRCm39) missense probably damaging 0.99
R5247:Kif15 UTSW 9 122,815,507 (GRCm39) missense possibly damaging 0.65
R5376:Kif15 UTSW 9 122,823,036 (GRCm39) missense probably benign 0.04
R5392:Kif15 UTSW 9 122,825,360 (GRCm39) missense probably damaging 0.99
R5422:Kif15 UTSW 9 122,813,954 (GRCm39) splice site probably null
R5562:Kif15 UTSW 9 122,807,081 (GRCm39) missense probably damaging 1.00
R5663:Kif15 UTSW 9 122,820,916 (GRCm39) splice site probably null
R5767:Kif15 UTSW 9 122,843,039 (GRCm39) missense possibly damaging 0.78
R5927:Kif15 UTSW 9 122,846,326 (GRCm39) missense probably benign 0.00
R6049:Kif15 UTSW 9 122,840,687 (GRCm39) missense probably damaging 0.98
R6435:Kif15 UTSW 9 122,815,556 (GRCm39) missense probably damaging 1.00
R7040:Kif15 UTSW 9 122,840,679 (GRCm39) missense possibly damaging 0.67
R7158:Kif15 UTSW 9 122,828,379 (GRCm39) missense probably benign
R7163:Kif15 UTSW 9 122,846,722 (GRCm39) missense probably damaging 1.00
R7197:Kif15 UTSW 9 122,838,991 (GRCm39) critical splice donor site probably null
R7318:Kif15 UTSW 9 122,817,014 (GRCm39) missense probably damaging 1.00
R7360:Kif15 UTSW 9 122,820,202 (GRCm39) missense probably benign
R8039:Kif15 UTSW 9 122,836,490 (GRCm39) missense possibly damaging 0.82
R8228:Kif15 UTSW 9 122,821,041 (GRCm39) missense possibly damaging 0.82
R8549:Kif15 UTSW 9 122,815,236 (GRCm39) missense probably benign
R9001:Kif15 UTSW 9 122,826,855 (GRCm39) missense probably benign 0.00
R9031:Kif15 UTSW 9 122,846,492 (GRCm39) intron probably benign
R9044:Kif15 UTSW 9 122,840,781 (GRCm39) missense probably benign 0.01
R9063:Kif15 UTSW 9 122,833,706 (GRCm39) missense probably damaging 1.00
R9306:Kif15 UTSW 9 122,807,056 (GRCm39) missense probably damaging 1.00
R9490:Kif15 UTSW 9 122,788,203 (GRCm39) missense probably benign 0.10
R9554:Kif15 UTSW 9 122,828,585 (GRCm39) missense probably damaging 1.00
R9682:Kif15 UTSW 9 122,815,712 (GRCm39) missense probably damaging 0.98
R9752:Kif15 UTSW 9 122,824,890 (GRCm39) critical splice donor site probably null
Z1177:Kif15 UTSW 9 122,780,116 (GRCm39) unclassified probably benign
Predicted Primers PCR Primer
(F):5'- AGCTCAGAGCTGAGGTTGTTGC -3'
(R):5'- GGTTGCTCCAGGAAATCAGAGAACG -3'

Sequencing Primer
(F):5'- GTGAGCTACATCACCAATTGTC -3'
(R):5'- CGGGACAGGAAGCAAAGAC -3'
Posted On 2013-08-06