Mutagenetix > Incidental Mutation

Incidental Mutation 'T0975:Or13g1'

67721

Institutional Source

Beutler Lab

Gene Symbol

Or13g1

Ensembl Gene

ENSMUSG00000054054

Gene Name

olfactory receptor family 13 subfamily G member 1

Synonyms

MOR251-4P, GA_x6K02T2NHDJ-9801340-9802266, Olfr309

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.058) question?

Stock #

T0975 (G3) of strain 714

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

85955393-85956319 bp(-) (GRCm39)

Type of Mutation

nonsense

DNA Base Change (assembly)

A to T at 85955492 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Tyrosine to Stop codon at position 276 (Y276*)

Ref Sequence

ENSEMBL: ENSMUSP00000134377 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000055690] [ENSMUST00000174158]

AlphaFold

Q7TS01

Predicted Effect

probably null
Transcript: ENSMUST00000055690
AA Change: Y276*

SMART Domains

Protein: ENSMUSP00000061929
Gene: ENSMUSG00000054054
AA Change: Y276*

Domain	Start	End	E-Value	Type
Pfam:7TM_GPCR_Srsx	33	303	4.5e-11	PFAM
Pfam:7tm_1	39	288	1.5e-26	PFAM
Pfam:7tm_4	137	281	7.9e-44	PFAM

Predicted Effect

probably null
Transcript: ENSMUST00000174158
AA Change: Y276*

SMART Domains

Protein: ENSMUSP00000134377
Gene: ENSMUSG00000054054
AA Change: Y276*

Domain	Start	End	E-Value	Type
Pfam:7tm_4	29	305	1.6e-55	PFAM
Pfam:7TM_GPCR_Srsx	33	303	4.5e-11	PFAM
Pfam:7tm_1	39	288	2.4e-18	PFAM

Coding Region Coverage

1x: 99.4%
3x: 98.7%
10x: 97.0%
20x: 93.3%

Validation Efficiency

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 67 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930556J24Rik	TAA	TAAA	11: 3,887,945 (GRCm39)		probably null	Het
4930556J24Rik	C	T	11: 3,926,324 (GRCm39)	A27T	unknown	Het
Ago3	C	T	4: 126,298,056 (GRCm39)	V155I	probably benign	Het
Ago3	G	A	4: 126,298,103 (GRCm39)	A139V	probably benign	Het
Ago3	C	T	4: 126,298,098 (GRCm39)	A141T	probably benign	Het
Ahdc1	ACCTCCT	ACCTCCTCCT	4: 132,790,065 (GRCm39)		probably benign	Het
Azin2	A	G	4: 128,839,927 (GRCm39)	Y222H	probably benign	Het
Bpifb5	C	A	2: 154,071,384 (GRCm39)		probably null	Het
Castor1	G	C	11: 4,170,445 (GRCm39)	G147A	probably benign	Het
Ccdc157	C	T	11: 4,096,246 (GRCm39)	A455T	probably damaging	Het
Ccng1	A	C	11: 40,644,871 (GRCm39)	S9A	probably benign	Het
Cfh	T	C	1: 140,082,336 (GRCm39)	T164A	probably benign	Het
Cherp	TTGGACCTGGACCTGGACCTGGACCTGGA	TTGGACCTGGACCTGGACCTGGA	8: 73,215,878 (GRCm39)		probably benign	Het
Chrng	T	C	1: 87,138,348 (GRCm39)	S380P	probably benign	Het
Clspn	ACGGCGGCGGCGGCG	ACGGCGGCGGCGGCGGCGGCG	4: 126,460,230 (GRCm39)		probably benign	Het
Ctrc	T	TA	4: 141,572,507 (GRCm39)		probably null	Het
Cxxc1	C	T	18: 74,353,992 (GRCm39)	R593C	probably damaging	Het
Dlgap1	T	C	17: 70,823,950 (GRCm39)	S312P	possibly damaging	Het
Dnah10	A	G	5: 124,840,130 (GRCm39)	S1255G	probably benign	Het
Dpep1	A	T	8: 123,927,727 (GRCm39)	S388C	probably damaging	Het
Emid1	T	C	11: 5,094,386 (GRCm39)	T42A	probably damaging	Het
Emid1	A	C	11: 5,078,884 (GRCm39)	L353V	probably benign	Het
Epn3	A	G	11: 94,382,733 (GRCm39)		probably null	Het
Fam124b	T	C	1: 80,190,843 (GRCm39)	E180G	probably benign	Het
Fam135b	T	G	15: 71,335,734 (GRCm39)	T487P	probably damaging	Het
Gja4	G	C	4: 127,206,024 (GRCm39)	H246Q	probably benign	Het
Gm9972	GA	GAA	11: 42,927,597 (GRCm39)		probably null	Het
Hmmr	G	C	11: 40,614,243 (GRCm39)	N148K	probably damaging	Het
Homez	C	T	14: 55,094,796 (GRCm39)	R304K	possibly damaging	Het
Ifngr1	G	A	10: 19,485,221 (GRCm39)	V407M	probably damaging	Het
Inpp5j	G	T	11: 3,452,527 (GRCm39)	T241N	possibly damaging	Het
Kif12	GGGGC	GGGGCCTCCACCCGGCGGGC	4: 63,089,660 (GRCm39)		probably benign	Het
Kremen1	C	T	11: 5,145,105 (GRCm39)	A424T	probably benign	Het
Mat2b	G	A	11: 40,570,918 (GRCm39)	T302I	probably benign	Het
Mtmr3	C	T	11: 4,438,441 (GRCm39)	R671K	probably benign	Het
Nacad	A	G	11: 6,551,632 (GRCm39)	C520R	probably benign	Het
Nacad	GCAGGGTCAGGGTC	GCAGGGTCAGGGTCAGGGTC	11: 6,549,750 (GRCm39)		probably benign	Het
Nacad	T	C	11: 6,551,622 (GRCm39)	N523S	probably benign	Het
Nefh	G	A	11: 4,890,151 (GRCm39)	P823S	probably benign	Het
Nfrkb	G	C	9: 31,308,379 (GRCm39)	A230P	probably benign	Het
Nlrp4a	A	G	7: 26,149,062 (GRCm39)	E223G	probably damaging	Het
Notch3	T	A	17: 32,365,391 (GRCm39)	Y1107F	probably damaging	Het
Or10ak9	G	T	4: 118,726,500 (GRCm39)	R174M	probably benign	Het
Or6c35	A	G	10: 129,169,314 (GRCm39)	D188G	probably benign	Het
Osm	A	G	11: 4,189,588 (GRCm39)	D124G	probably benign	Het
Plekhm2	TTCCTCCTCCT	TTCCTCCT	4: 141,359,292 (GRCm39)		probably benign	Het
Pomgnt1	C	T	4: 115,994,624 (GRCm39)		probably benign	Het
Spen	A	G	4: 141,201,664 (GRCm39)	V2321A	probably benign	Het
Sytl1	TCTGC	TC	4: 132,984,305 (GRCm39)		probably benign	Het
Tcn2	G	C	11: 3,873,487 (GRCm39)	F286L	possibly damaging	Het
Tg	T	C	15: 66,560,712 (GRCm39)	S10P	probably benign	Het
Tmprss7	C	T	16: 45,501,096 (GRCm39)	R235Q	probably benign	Het
Tns3	G	A	11: 8,499,100 (GRCm39)		probably benign	Het
Tns3	G	T	11: 8,401,146 (GRCm39)	L1051M	probably benign	Het
Tns3	T	G	11: 8,429,518 (GRCm39)	E806A	probably benign	Het
Toe1	T	C	4: 116,663,290 (GRCm39)	I62M	probably benign	Het
Txnrd2	A	G	16: 18,294,315 (GRCm39)	H436R	probably damaging	Het
Ubr4	C	T	4: 139,179,092 (GRCm39)	P2001S	probably damaging	Het
Vmn2r23	T	A	6: 123,690,120 (GRCm39)	M332K	probably benign	Het
Zbtb8a	GG	GGATG	4: 129,253,812 (GRCm39)		probably benign	Het
Zbtb8a	T	C	4: 129,254,005 (GRCm39)	H163R	probably benign	Het
Zfyve21	A	G	12: 111,794,067 (GRCm39)	D206G	probably damaging	Het
Zkscan4	AGAGGAG	AGAG	13: 21,663,370 (GRCm39)		probably benign	Het
Zmym1	A	C	4: 126,943,466 (GRCm39)	H307Q	probably benign	Het
Zmym1	C	T	4: 126,941,740 (GRCm39)	D785N	probably benign	Het
Zmym1	C	T	4: 126,942,043 (GRCm39)	V684I	probably benign	Het
Zpld2	GTG	GTGCTG	4: 133,929,940 (GRCm39)		probably benign	Het

Other mutations in Or13g1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00886:Or13g1	APN	7	85,956,259 (GRCm39)	missense	probably damaging	1.00
R0370:Or13g1	UTSW	7	85,956,057 (GRCm39)	missense	probably benign	0.42
R1869:Or13g1	UTSW	7	85,955,875 (GRCm39)	missense	possibly damaging	0.96
R2047:Or13g1	UTSW	7	85,956,012 (GRCm39)	missense	probably damaging	1.00
R3939:Or13g1	UTSW	7	85,955,437 (GRCm39)	missense	probably benign	0.00
R4562:Or13g1	UTSW	7	85,956,360 (GRCm39)	start gained	probably benign
R4640:Or13g1	UTSW	7	85,956,274 (GRCm39)	missense	probably benign	0.01
R4811:Or13g1	UTSW	7	85,956,166 (GRCm39)	missense	probably benign	0.41
R4867:Or13g1	UTSW	7	85,955,491 (GRCm39)	missense	probably benign	0.00
R4954:Or13g1	UTSW	7	85,955,809 (GRCm39)	missense	probably benign	0.01
R5784:Or13g1	UTSW	7	85,955,743 (GRCm39)	missense	probably damaging	1.00
R6043:Or13g1	UTSW	7	85,955,547 (GRCm39)	missense	probably damaging	0.97
R6498:Or13g1	UTSW	7	85,956,226 (GRCm39)	missense	probably benign
R6849:Or13g1	UTSW	7	85,956,248 (GRCm39)	missense	possibly damaging	0.54
R7238:Or13g1	UTSW	7	85,955,799 (GRCm39)	missense	probably damaging	1.00
R7811:Or13g1	UTSW	7	85,955,554 (GRCm39)	missense	probably damaging	1.00
R7917:Or13g1	UTSW	7	85,955,686 (GRCm39)	missense	probably damaging	1.00
R8010:Or13g1	UTSW	7	85,956,260 (GRCm39)	missense	probably benign	0.24
R8271:Or13g1	UTSW	7	85,955,962 (GRCm39)	missense	probably benign	0.13
R9017:Or13g1	UTSW	7	85,955,957 (GRCm39)	nonsense	probably null
R9150:Or13g1	UTSW	7	85,955,942 (GRCm39)	missense	probably damaging	0.96

Predicted Primers

PCR Primer
(F):5'- TGCCTACATTGGTAAATGAGTGTCTGG -3'
(R):5'- CCTGGCTGTGGGAGACTTTACTCTAAC -3'

Sequencing Primer
(F):5'- GGATCATTTCCCAAAAAACCTGTG -3'
(R):5'- GGAGACTTTACTCTAACCTGCATTTC -3'

Posted On

2013-09-03