Mutagenetix > Incidental Mutation

Incidental Mutation 'R0748:Adgrf3'

70221

Institutional Source

Beutler Lab

Gene Symbol

Adgrf3

Ensembl Gene

ENSMUSG00000067642

Gene Name

adhesion G protein-coupled receptor F3

Synonyms

PGR23, LOC381628, Gpr113

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R0748 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

30398429-30410720 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 30401874 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Valine to Alanine at position 718 (V718A)

Ref Sequence

ENSEMBL: ENSMUSP00000085440 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000088117] [ENSMUST00000125367]

AlphaFold

Q58Y75

Predicted Effect

probably damaging
Transcript: ENSMUST00000088117
AA Change: V718A

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000085440
Gene: ENSMUSG00000067642
AA Change: V718A

Domain	Start	End	E-Value	Type
signal peptide	1	20	N/A	INTRINSIC
low complexity region	85	97	N/A	INTRINSIC
Blast:IG	163	252	2e-20	BLAST
Blast:CCP	341	399	1e-6	BLAST
low complexity region	403	415	N/A	INTRINSIC
low complexity region	471	483	N/A	INTRINSIC
GPS	632	684	2.68e-17	SMART
Pfam:7tm_2	687	935	1e-30	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000125367

SMART Domains

Protein: ENSMUSP00000120958
Gene: ENSMUSG00000067642

Domain	Start	End	E-Value	Type
transmembrane domain	21	43	N/A	INTRINSIC

Meta Mutation Damage Score

0.1613

Coding Region Coverage

1x: 99.1%
3x: 98.5%
10x: 96.7%
20x: 92.4%

Validation Efficiency

100% (36/36)

Allele List at MGI

Other mutations in this stock

Total: 29 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Amigo1	A	G	3: 108,095,946 (GRCm39)	S482G	probably damaging	Het
F2rl3	A	G	8: 73,489,379 (GRCm39)	Q202R	probably benign	Het
Faap100	A	G	11: 120,262,997 (GRCm39)	V787A	probably damaging	Het
Fat4	A	T	3: 38,941,977 (GRCm39)	Q290L	possibly damaging	Het
Flnc	A	G	6: 29,446,343 (GRCm39)	E920G	probably damaging	Het
Gm9881	T	C	16: 90,967,313 (GRCm39)	N137S	unknown	Het
Hps4	A	G	5: 112,522,780 (GRCm39)	E546G	probably damaging	Het
Htr2b	A	G	1: 86,038,528 (GRCm39)	I26T	probably benign	Het
Insr	A	C	8: 3,308,841 (GRCm39)	M65R	probably damaging	Het
Kif20a	T	C	18: 34,761,241 (GRCm39)		probably benign	Het
L3mbtl1	A	T	2: 162,813,083 (GRCm39)		probably benign	Het
L3mbtl1	A	C	2: 162,813,084 (GRCm39)		probably null	Het
Lcn4	T	C	2: 26,558,359 (GRCm39)	I175M	probably damaging	Het
Malt1	T	A	18: 65,608,331 (GRCm39)		probably null	Het
Nufip1	T	C	14: 76,348,508 (GRCm39)	S46P	probably damaging	Het
Nup93	T	A	8: 95,034,571 (GRCm39)	Y629N	probably damaging	Het
Or10a49	C	A	7: 108,468,357 (GRCm39)	M1I	probably null	Het
Pcsk6	T	C	7: 65,688,716 (GRCm39)		probably benign	Het
Rdx	G	C	9: 51,976,160 (GRCm39)	V33L	possibly damaging	Het
Rnf213	T	C	11: 119,364,306 (GRCm39)	L4535P	probably damaging	Het
Rorb	T	A	19: 18,955,164 (GRCm39)	T66S	probably damaging	Het
S100a9	T	C	3: 90,600,198 (GRCm39)	D66G	possibly damaging	Het
Sacs	T	C	14: 61,446,714 (GRCm39)	I2920T	probably damaging	Het
Safb2	T	C	17: 56,882,580 (GRCm39)	N351S	probably benign	Het
Ugt1a10	C	T	1: 88,142,845 (GRCm39)	P113L	probably damaging	Het
Unc13b	G	A	4: 43,241,164 (GRCm39)		probably benign	Het
Vars1	A	G	17: 35,216,988 (GRCm39)	S489P	probably damaging	Het
Wars2	A	G	3: 99,123,888 (GRCm39)	K250E	probably damaging	Het
Zfp292	A	G	4: 34,816,424 (GRCm39)		probably benign	Het

Other mutations in Adgrf3

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL03080:Adgrf3	APN	5	30,401,827 (GRCm39)	missense	probably benign	0.02
IGL03171:Adgrf3	APN	5	30,401,292 (GRCm39)	missense	probably damaging	1.00
R0010:Adgrf3	UTSW	5	30,410,607 (GRCm39)	splice site	probably benign
R0042:Adgrf3	UTSW	5	30,402,426 (GRCm39)	missense	probably damaging	1.00
R0140:Adgrf3	UTSW	5	30,401,379 (GRCm39)	missense	probably benign	0.19
R0617:Adgrf3	UTSW	5	30,400,078 (GRCm39)	missense	probably benign	0.25
R1291:Adgrf3	UTSW	5	30,404,532 (GRCm39)	missense	probably damaging	0.99
R1330:Adgrf3	UTSW	5	30,400,093 (GRCm39)	missense	probably benign	0.24
R1468:Adgrf3	UTSW	5	30,407,227 (GRCm39)	splice site	probably benign
R1695:Adgrf3	UTSW	5	30,408,553 (GRCm39)	missense	probably benign	0.05
R1716:Adgrf3	UTSW	5	30,402,549 (GRCm39)	missense	probably benign	0.03
R1844:Adgrf3	UTSW	5	30,404,211 (GRCm39)	missense	probably damaging	0.96
R1935:Adgrf3	UTSW	5	30,407,304 (GRCm39)	missense	probably benign	0.00
R1936:Adgrf3	UTSW	5	30,407,304 (GRCm39)	missense	probably benign	0.00
R2059:Adgrf3	UTSW	5	30,404,489 (GRCm39)	missense	possibly damaging	0.91
R2656:Adgrf3	UTSW	5	30,401,436 (GRCm39)	missense	possibly damaging	0.96
R2913:Adgrf3	UTSW	5	30,401,992 (GRCm39)	missense	probably damaging	1.00
R2914:Adgrf3	UTSW	5	30,401,992 (GRCm39)	missense	probably damaging	1.00
R2987:Adgrf3	UTSW	5	30,402,358 (GRCm39)	missense	probably damaging	1.00
R3797:Adgrf3	UTSW	5	30,401,821 (GRCm39)	missense	possibly damaging	0.49
R3798:Adgrf3	UTSW	5	30,401,821 (GRCm39)	missense	possibly damaging	0.49
R3799:Adgrf3	UTSW	5	30,401,821 (GRCm39)	missense	possibly damaging	0.49
R3934:Adgrf3	UTSW	5	30,405,432 (GRCm39)	unclassified	probably benign
R4043:Adgrf3	UTSW	5	30,409,360 (GRCm39)	missense	probably benign	0.00
R4080:Adgrf3	UTSW	5	30,402,367 (GRCm39)	nonsense	probably null
R4575:Adgrf3	UTSW	5	30,407,255 (GRCm39)	missense	probably benign	0.00
R4754:Adgrf3	UTSW	5	30,402,615 (GRCm39)	critical splice acceptor site	probably null
R4819:Adgrf3	UTSW	5	30,403,442 (GRCm39)	missense	possibly damaging	0.66
R4893:Adgrf3	UTSW	5	30,405,476 (GRCm39)	missense	probably benign	0.00
R4991:Adgrf3	UTSW	5	30,404,146 (GRCm39)	missense	probably benign	0.26
R5686:Adgrf3	UTSW	5	30,402,304 (GRCm39)	missense	probably damaging	1.00
R5965:Adgrf3	UTSW	5	30,410,637 (GRCm39)	missense	probably benign	0.00
R5997:Adgrf3	UTSW	5	30,403,360 (GRCm39)	critical splice donor site	probably null
R6103:Adgrf3	UTSW	5	30,401,265 (GRCm39)	missense	probably damaging	1.00
R6244:Adgrf3	UTSW	5	30,402,531 (GRCm39)	missense	probably benign	0.17
R6409:Adgrf3	UTSW	5	30,402,312 (GRCm39)	missense	probably damaging	0.96
R6575:Adgrf3	UTSW	5	30,401,522 (GRCm39)	missense	possibly damaging	0.72
R6745:Adgrf3	UTSW	5	30,408,601 (GRCm39)	missense	probably benign	0.31
R6790:Adgrf3	UTSW	5	30,401,385 (GRCm39)	missense	probably benign	0.00
R6813:Adgrf3	UTSW	5	30,402,519 (GRCm39)	missense	probably damaging	0.96
R7202:Adgrf3	UTSW	5	30,409,378 (GRCm39)	nonsense	probably null
R7250:Adgrf3	UTSW	5	30,400,680 (GRCm39)	missense	probably damaging	1.00
R7353:Adgrf3	UTSW	5	30,403,495 (GRCm39)	missense	probably damaging	0.98
R7634:Adgrf3	UTSW	5	30,407,245 (GRCm39)	missense	probably benign	0.01
R7658:Adgrf3	UTSW	5	30,402,204 (GRCm39)	missense	probably benign	0.41
R8037:Adgrf3	UTSW	5	30,404,510 (GRCm39)	missense	probably damaging	1.00
R8281:Adgrf3	UTSW	5	30,402,301 (GRCm39)	missense	possibly damaging	0.46
R8717:Adgrf3	UTSW	5	30,403,579 (GRCm39)	unclassified	probably benign
R8857:Adgrf3	UTSW	5	30,402,065 (GRCm39)	nonsense	probably null
R8926:Adgrf3	UTSW	5	30,405,446 (GRCm39)	missense	possibly damaging	0.46
R9391:Adgrf3	UTSW	5	30,400,071 (GRCm39)	missense	possibly damaging	0.94
R9446:Adgrf3	UTSW	5	30,401,957 (GRCm39)	missense	probably benign	0.01
R9522:Adgrf3	UTSW	5	30,404,482 (GRCm39)	missense	possibly damaging	0.90
Z1088:Adgrf3	UTSW	5	30,404,118 (GRCm39)	missense	possibly damaging	0.90

Predicted Primers

PCR Primer
(F):5'- TAGCCCAGCATCACCATCAGTGAG -3'
(R):5'- AGGTACATGCAGCCAATGCCAG -3'

Sequencing Primer
(F):5'- ACTACGTGCTTGGACAGC -3'
(R):5'- GCCAATGCCAGCATCACTC -3'

Posted On

2013-09-30