Mutagenetix > Incidental Mutation

Incidental Mutation 'R0773:Prl3b1'

76975

Institutional Source

Beutler Lab

Gene Symbol

Prl3b1

Ensembl Gene

ENSMUSG00000038891

Gene Name

prolactin family 3, subfamily b, member 1

Synonyms

mplII, PL, Pl-2, mPL-II, Pl2, prolactin-like, Csh2

MMRRC Submission

038953-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.074) question?

Stock #

R0773 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

27426413-27433666 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

G to A at 27427848 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Alanine to Threonine at position 53 (A53T)

Ref Sequence

ENSEMBL: ENSMUSP00000047680 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000035273] [ENSMUST00000225089]

AlphaFold

P09586

Predicted Effect

probably benign
Transcript: ENSMUST00000035273
AA Change: A53T

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)

SMART Domains

Protein: ENSMUSP00000047680
Gene: ENSMUSG00000038891
AA Change: A53T

Domain	Start	End	E-Value	Type
Pfam:Hormone_1	18	222	1e-55	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000225089
AA Change: A53T

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 99.1%
3x: 98.3%
10x: 95.2%
20x: 87.2%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 1 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Rasl10b	G	A	11: 83,308,665 (GRCm39)		probably null	Het

Other mutations in Prl3b1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02385:Prl3b1	APN	13	27,433,366 (GRCm39)	missense	possibly damaging	0.51
IGL02669:Prl3b1	APN	13	27,429,795 (GRCm39)	missense	probably benign	0.24
IGL03035:Prl3b1	APN	13	27,433,516 (GRCm39)	unclassified	probably benign
IGL03077:Prl3b1	APN	13	27,429,759 (GRCm39)	missense	probably benign	0.00
Gabby	UTSW	13	27,431,928 (GRCm39)	missense	probably damaging	1.00
peaches	UTSW	13	27,426,473 (GRCm39)	start codon destroyed	probably null	1.00
Pits	UTSW	13	27,431,957 (GRCm39)	critical splice donor site	probably null
R2014_Prl3b1_632	UTSW	13	27,431,948 (GRCm39)	missense	probably benign	0.00
R0716:Prl3b1	UTSW	13	27,427,779 (GRCm39)	missense	probably benign	0.02
R0758:Prl3b1	UTSW	13	27,427,848 (GRCm39)	missense	probably benign	0.00
R0774:Prl3b1	UTSW	13	27,427,848 (GRCm39)	missense	probably benign	0.00
R0775:Prl3b1	UTSW	13	27,427,848 (GRCm39)	missense	probably benign	0.00
R1364:Prl3b1	UTSW	13	27,427,848 (GRCm39)	missense	probably benign	0.00
R1366:Prl3b1	UTSW	13	27,427,848 (GRCm39)	missense	probably benign	0.00
R1367:Prl3b1	UTSW	13	27,427,848 (GRCm39)	missense	probably benign	0.00
R1368:Prl3b1	UTSW	13	27,427,848 (GRCm39)	missense	probably benign	0.00
R1530:Prl3b1	UTSW	13	27,427,848 (GRCm39)	missense	probably benign	0.00
R1884:Prl3b1	UTSW	13	27,431,886 (GRCm39)	missense	possibly damaging	0.95
R1990:Prl3b1	UTSW	13	27,429,775 (GRCm39)	missense	possibly damaging	0.94
R1991:Prl3b1	UTSW	13	27,431,895 (GRCm39)	missense	possibly damaging	0.60
R2014:Prl3b1	UTSW	13	27,431,948 (GRCm39)	missense	probably benign	0.00
R2885:Prl3b1	UTSW	13	27,433,505 (GRCm39)	missense	probably damaging	1.00
R4259:Prl3b1	UTSW	13	27,427,889 (GRCm39)	splice site	probably null
R4580:Prl3b1	UTSW	13	27,433,450 (GRCm39)	missense	possibly damaging	0.93
R4913:Prl3b1	UTSW	13	27,433,460 (GRCm39)	missense	probably damaging	0.99
R5897:Prl3b1	UTSW	13	27,429,858 (GRCm39)	missense	probably benign	0.08
R6235:Prl3b1	UTSW	13	27,431,928 (GRCm39)	missense	probably damaging	1.00
R6366:Prl3b1	UTSW	13	27,427,875 (GRCm39)	missense	probably benign	0.00
R6597:Prl3b1	UTSW	13	27,431,957 (GRCm39)	critical splice donor site	probably null
R7179:Prl3b1	UTSW	13	27,427,827 (GRCm39)	missense	probably benign	0.05
R7312:Prl3b1	UTSW	13	27,426,473 (GRCm39)	start codon destroyed	probably null	1.00
X0026:Prl3b1	UTSW	13	27,431,906 (GRCm39)	missense	probably benign	0.21
Z1177:Prl3b1	UTSW	13	27,427,742 (GRCm39)	missense	probably benign	0.23

Predicted Primers

PCR Primer
(F):5'- GGACAGACAGTATAGCCTCTACAGTGT -3'
(R):5'- AGGGTTTGATGATTGCTCCATTGGAA -3'

Sequencing Primer
(F):5'- CAGTATAGCCTCTACAGTGTCATTTG -3'
(R):5'- ggaggaggtgagaggtgg -3'

Posted On

2013-10-16