Mutagenetix > Incidental Mutation

Incidental Mutation 'R0940:Fmo6'

81357

Institutional Source

Beutler Lab

Gene Symbol

Fmo6

Ensembl Gene

ENSMUSG00000095576

Gene Name

flavin containing monooxygenase 6

Synonyms

MMRRC Submission

039079-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.068) question?

Stock #

R0940 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

162744120-162765084 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 162753795 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Cysteine to Serine at position 116 (C116S)

Ref Sequence

ENSEMBL: ENSMUSP00000136378 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000178465] [ENSMUST00000195576]

AlphaFold

J3QMN6

Predicted Effect

probably benign
Transcript: ENSMUST00000178465
AA Change: C116S

PolyPhen 2 Score 0.067 (Sensitivity: 0.94; Specificity: 0.84)

SMART Domains

Protein: ENSMUSP00000136378
Gene: ENSMUSG00000095576
AA Change: C116S

Domain	Start	End	E-Value	Type
Pfam:FMO-like	2	532	3.2e-273	PFAM
Pfam:Pyr_redox_2	3	226	2e-13	PFAM
Pfam:Pyr_redox_3	6	220	4.6e-17	PFAM
Pfam:K_oxygenase	79	223	1e-7	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000195576

SMART Domains

Protein: ENSMUSP00000144106
Gene: ENSMUSG00000095576

Domain	Start	End	E-Value	Type
Pfam:FMO-like	2	118	5.1e-58	PFAM
Pfam:NAD_binding_8	7	84	2.2e-5	PFAM

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 99.5%
3x: 99.1%
10x: 98.0%
20x: 96.7%

Validation Efficiency

100% (68/68)

Allele List at MGI

Other mutations in this stock

Total: 63 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1810064F22Rik	C	T	9: 22,119,367 (GRCm39)		noncoding transcript	Het
2610021A01Rik	T	G	7: 41,275,858 (GRCm39)	I520M	probably damaging	Het
Ackr4	A	G	9: 103,976,831 (GRCm39)	F39L	probably damaging	Het
Adgre5	C	T	8: 84,460,126 (GRCm39)	S92N	probably damaging	Het
Adrb2	T	C	18: 62,312,762 (GRCm39)	D21G	probably benign	Het
Akr1c6	G	A	13: 4,486,372 (GRCm39)	E60K	probably benign	Het
Bcl7c	T	A	7: 127,306,503 (GRCm39)	N96I	possibly damaging	Het
Brca1	A	T	11: 101,422,969 (GRCm39)	S106R	possibly damaging	Het
C6	A	T	15: 4,764,717 (GRCm39)	T138S	probably benign	Het
Cul3	T	C	1: 80,300,564 (GRCm39)		probably benign	Het
Dnah8	T	A	17: 31,022,217 (GRCm39)	M3939K	probably damaging	Het
Dock4	T	A	12: 40,681,626 (GRCm39)		probably benign	Het
Dsc2	T	G	18: 20,183,116 (GRCm39)	T101P	probably damaging	Het
Dynlt1b	T	C	17: 6,697,649 (GRCm39)		probably benign	Het
E330013P04Rik	A	G	19: 60,150,354 (GRCm39)		noncoding transcript	Het
Fggy	A	G	4: 95,585,238 (GRCm39)	E39G	probably benign	Het
Fhip1a	A	G	3: 85,572,797 (GRCm39)	V952A	possibly damaging	Het
Gadd45a	A	G	6: 67,013,813 (GRCm39)	I44T	possibly damaging	Het
Gmps	A	G	3: 63,883,743 (GRCm39)		probably benign	Het
Gnmt	A	G	17: 47,037,271 (GRCm39)	L171P	probably damaging	Het
Hnrnpm	G	A	17: 33,868,976 (GRCm39)	R523C	probably damaging	Het
Inpp5a	T	C	7: 139,105,654 (GRCm39)	Y202H	probably damaging	Het
Kank3	C	T	17: 34,036,450 (GRCm39)	S106F	probably damaging	Het
Lmcd1	A	G	6: 112,305,658 (GRCm39)	D253G	probably benign	Het
Lrrk2	A	T	15: 91,613,284 (GRCm39)	I803F	possibly damaging	Het
Mybpc2	T	C	7: 44,156,311 (GRCm39)	K834R	probably benign	Het
Mycbp2	A	T	14: 103,500,129 (GRCm39)		probably benign	Het
Myh4	A	T	11: 67,133,689 (GRCm39)	N243Y	probably damaging	Het
Myorg	A	G	4: 41,497,996 (GRCm39)	Y545H	probably damaging	Het
Nfatc1	C	T	18: 80,679,110 (GRCm39)	M759I	probably benign	Het
Nipal4	A	G	11: 46,041,139 (GRCm39)	I352T	possibly damaging	Het
Nomo1	A	G	7: 45,683,329 (GRCm39)	E25G	possibly damaging	Het
Or10j27	A	G	1: 172,958,020 (GRCm39)	S255P	probably benign	Het
Or13a19	T	C	7: 139,903,065 (GRCm39)	I151T	probably benign	Het
Or14j7	A	G	17: 38,234,591 (GRCm39)	I45V	probably damaging	Het
Or1e32	T	C	11: 73,705,050 (GRCm39)	N286S	probably damaging	Het
Or4c116	T	A	2: 88,942,419 (GRCm39)	I146L	probably benign	Het
Or5p66	T	C	7: 107,886,264 (GRCm39)	D23G	probably benign	Het
Pabpn1l	A	G	8: 123,349,183 (GRCm39)	V78A	probably benign	Het
Pde6b	T	A	5: 108,568,203 (GRCm39)	I327N	possibly damaging	Het
Phrf1	T	C	7: 140,834,768 (GRCm39)		probably benign	Het
Pkm	C	T	9: 59,575,818 (GRCm39)		probably benign	Het
Plxna2	G	A	1: 194,482,863 (GRCm39)	V1519I	probably benign	Het
Ppp2cb	T	C	8: 34,105,689 (GRCm39)		probably null	Het
Prickle2	A	G	6: 92,387,984 (GRCm39)	Y473H	probably benign	Het
Prpf3	A	G	3: 95,751,535 (GRCm39)	W389R	probably damaging	Het
Psme4	G	A	11: 30,765,264 (GRCm39)	E544K	possibly damaging	Het
Relb	A	T	7: 19,345,767 (GRCm39)	D395E	probably damaging	Het
Rif1	GCCACCA	GCCA	2: 52,000,336 (GRCm39)		probably benign	Het
Rnf213	A	G	11: 119,307,389 (GRCm39)	N683S	probably benign	Het
Rtel1	T	C	2: 180,964,596 (GRCm39)	C102R	probably benign	Het
Sel1l3	C	T	5: 53,301,379 (GRCm39)		probably benign	Het
Slc8a2	A	G	7: 15,878,887 (GRCm39)	T458A	probably benign	Het
Smc3	T	A	19: 53,629,340 (GRCm39)	M931K	probably benign	Het
Sorbs2	T	C	8: 46,249,539 (GRCm39)	V795A	probably benign	Het
Tgm3	A	G	2: 129,854,326 (GRCm39)	S2G	probably benign	Het
Tpbpa	T	C	13: 61,087,867 (GRCm39)	T75A	probably damaging	Het
Trub1	A	G	19: 57,473,495 (GRCm39)		probably benign	Het
Uggt2	A	G	14: 119,328,604 (GRCm39)		probably null	Het
Ugt2a3	A	G	5: 87,475,065 (GRCm39)	V393A	possibly damaging	Het
Vav3	T	A	3: 109,470,151 (GRCm39)	M532K	possibly damaging	Het
Zfp616	A	T	11: 73,975,850 (GRCm39)	K706N	probably damaging	Het
Zkscan1	T	C	5: 138,091,432 (GRCm39)	F55S	probably damaging	Het

Other mutations in Fmo6

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01392:Fmo6	APN	1	162,757,580 (GRCm39)	nonsense	probably null
IGL02083:Fmo6	APN	1	162,748,033 (GRCm39)	nonsense	probably null
adventure	UTSW	1	162,750,379 (GRCm39)	missense	probably benign
R0792:Fmo6	UTSW	1	162,748,132 (GRCm39)	missense	probably damaging	0.98
R1173:Fmo6	UTSW	1	162,753,710 (GRCm39)	missense	probably damaging	1.00
R1268:Fmo6	UTSW	1	162,748,086 (GRCm39)	missense	probably damaging	1.00
R1538:Fmo6	UTSW	1	162,753,675 (GRCm39)	missense	probably damaging	1.00
R1694:Fmo6	UTSW	1	162,750,241 (GRCm39)	missense	probably benign
R1717:Fmo6	UTSW	1	162,753,821 (GRCm39)	nonsense	probably null
R1837:Fmo6	UTSW	1	162,750,379 (GRCm39)	missense	probably benign
R2125:Fmo6	UTSW	1	162,757,527 (GRCm39)	missense	possibly damaging	0.82
R2434:Fmo6	UTSW	1	162,744,439 (GRCm39)	missense	probably benign	0.00
R2894:Fmo6	UTSW	1	162,750,293 (GRCm39)	nonsense	probably null
R4864:Fmo6	UTSW	1	162,751,964 (GRCm39)	missense	probably benign	0.34
R6414:Fmo6	UTSW	1	162,748,014 (GRCm39)	missense	probably damaging	0.97
R6576:Fmo6	UTSW	1	162,750,264 (GRCm39)	missense	probably damaging	1.00
R6883:Fmo6	UTSW	1	162,757,461 (GRCm39)	missense	probably damaging	1.00
R7013:Fmo6	UTSW	1	162,745,817 (GRCm39)	missense	probably benign	0.00
R7014:Fmo6	UTSW	1	162,753,877 (GRCm39)	missense	probably benign	0.13
R7657:Fmo6	UTSW	1	162,750,285 (GRCm39)	missense	probably benign
R8351:Fmo6	UTSW	1	162,748,174 (GRCm39)	missense	probably damaging	1.00
R8451:Fmo6	UTSW	1	162,748,174 (GRCm39)	missense	probably damaging	1.00
R8911:Fmo6	UTSW	1	162,748,114 (GRCm39)	missense	possibly damaging	0.80
R9217:Fmo6	UTSW	1	162,748,046 (GRCm39)	missense	probably benign	0.02
R9274:Fmo6	UTSW	1	162,747,921 (GRCm39)	missense	probably benign	0.00
X0025:Fmo6	UTSW	1	162,750,427 (GRCm39)	missense	probably damaging	1.00
X0025:Fmo6	UTSW	1	162,748,065 (GRCm39)	missense	probably benign	0.29
Z1176:Fmo6	UTSW	1	162,753,701 (GRCm39)	nonsense	probably null

Predicted Primers

PCR Primer
(F):5'- TCAGAGACCCCATGTGTGTTAGGAC -3'
(R):5'- AAAGTGGAGTCACTGTCGAGCAGC -3'

Sequencing Primer
(F):5'- ATGTTTGCATGACCCAGTGAC -3'
(R):5'- TCACTAAGGATGTGCTCTGAC -3'

Posted On

2013-11-07