Mutagenetix > Incidental Mutation

Incidental Mutation 'R0959:Tg'

81797

Institutional Source

Beutler Lab

Gene Symbol

Ensembl Gene

ENSMUSG00000053469

Gene Name

thyroglobulin

Synonyms

Tgn

MMRRC Submission

039088-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.096) question?

Stock #

R0959 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

66542606-66722570 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 66579859 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Serine at position 1555 (T1555S)

Ref Sequence

ENSEMBL: ENSMUSP00000070239 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000065916] [ENSMUST00000163495] [ENSMUST00000171045]

AlphaFold

no structure available at present

Predicted Effect

probably damaging
Transcript: ENSMUST00000065916
AA Change: T1555S

PolyPhen 2 Score 0.983 (Sensitivity: 0.75; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000070239
Gene: ENSMUSG00000053469
AA Change: T1555S

Domain	Start	End	E-Value	Type
signal peptide	1	20	N/A	INTRINSIC
TY	50	97	5.9e-16	SMART
TY	118	165	5.59e-17	SMART
Pfam:Thyroglobulin_1	174	252	4e-9	PFAM
TY	317	363	4.36e-19	SMART
low complexity region	495	504	N/A	INTRINSIC
TY	617	662	3.58e-15	SMART
TY	684	730	1.47e-16	SMART
TY	880	926	1.51e-4	SMART
TY	1029	1078	1.21e-12	SMART
TY	1106	1150	7.56e-5	SMART
TY	1167	1215	7.26e-16	SMART
low complexity region	1244	1255	N/A	INTRINSIC
Pfam:GCC2_GCC3	1464	1509	2.7e-16	PFAM
TY	1519	1568	9.81e-13	SMART
Pfam:COesterase	2181	2717	8.4e-140	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000163495

SMART Domains

Protein: ENSMUSP00000129868
Gene: ENSMUSG00000053469

Domain	Start	End	E-Value	Type
TY	14	63	1.21e-12	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000167512

Predicted Effect

SMART Domains

Protein: ENSMUSP00000126454
Gene: ENSMUSG00000053469
AA Change: T68S

Domain	Start	End	E-Value	Type
internal_repeat_1	93	331	1.53e-6	PROSPERO
Pfam:COesterase	562	1098	2.1e-137	PFAM

Coding Region Coverage

1x: 99.6%
3x: 98.7%
10x: 95.8%
20x: 89.6%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] Thyroglobulin (Tg) is a glycoprotein homodimer produced predominantly by the thryroid gland. It acts as a substrate for the synthesis of thyroxine and triiodothyronine as well as the storage of the inactive forms of thyroid hormone and iodine. Thyroglobulin is secreted from the endoplasmic reticulum to its site of iodination, and subsequent thyroxine biosynthesis, in the follicular lumen. Mutations in this gene cause thyroid dyshormonogenesis, manifested as goiter, and are associated with moderate to severe congenital hypothyroidism. Polymorphisms in this gene are associated with susceptibility to autoimmune thyroid diseases (AITD) such as Graves disease and Hashimoto thryoiditis. [provided by RefSeq, Nov 2009]
PHENOTYPE: Mice homozygous for a spontaneous mutation exhibit enlarged thyroid gland, hypothyroidism, abnormal thyroid gland morphology, and decreased body weight. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 57 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Actl11	T	C	9: 107,808,434 (GRCm39)	V919A	probably damaging	Het
Adam28	G	A	14: 68,845,387 (GRCm39)	P761L	possibly damaging	Het
Aoc1l3	T	A	6: 48,965,566 (GRCm39)	C525S	possibly damaging	Het
Aplf	G	A	6: 87,623,065 (GRCm39)	P338L	probably benign	Het
Arl5b	T	A	2: 15,077,942 (GRCm39)	I89N	probably damaging	Het
Asap2	T	C	12: 21,297,320 (GRCm39)	V596A	probably damaging	Het
Baz1b	T	G	5: 135,273,076 (GRCm39)	F1400C	probably damaging	Het
Ccdc149	A	T	5: 52,542,497 (GRCm39)	L365Q	probably damaging	Het
Ccdc60	A	G	5: 116,318,870 (GRCm39)	S149P	probably damaging	Het
Ces1b	A	G	8: 93,794,775 (GRCm39)	C275R	probably damaging	Het
Creb3	T	C	4: 43,563,509 (GRCm39)	L163P	probably damaging	Het
Dhx37	T	G	5: 125,500,496 (GRCm39)	N570T	probably benign	Het
Epc1	T	C	18: 6,453,657 (GRCm39)	N223D	probably damaging	Het
Gbp5	C	T	3: 142,208,885 (GRCm39)	H143Y	possibly damaging	Het
Gfod1	A	T	13: 43,456,905 (GRCm39)	D23E	probably benign	Het
Gm7361	A	T	5: 26,467,051 (GRCm39)	E223D	possibly damaging	Het
Izumo1	A	G	7: 45,274,415 (GRCm39)	K161E	probably damaging	Het
Kcnh2	C	T	5: 24,527,670 (GRCm39)	R894H	probably damaging	Het
Lrp1b	T	C	2: 41,158,366 (GRCm39)	N1617S	possibly damaging	Het
Med13l	A	T	5: 118,892,350 (GRCm39)	E1924D	possibly damaging	Het
Mroh2a	GCCC	GC	1: 88,159,979 (GRCm39)		probably null	Het
Mtg2	T	G	2: 179,725,221 (GRCm39)	S145A	probably benign	Het
Mug2	G	A	6: 122,062,454 (GRCm39)	S1442N	probably benign	Het
Myo1b	A	T	1: 51,836,246 (GRCm39)	I315N	probably damaging	Het
Naip2	G	A	13: 100,291,386 (GRCm39)	T1184M	probably benign	Het
Naip2	T	A	13: 100,291,419 (GRCm39)	H1173L	probably benign	Het
Nsrp1	G	A	11: 76,937,285 (GRCm39)	R304*	probably null	Het
Or2a56	G	T	6: 42,932,686 (GRCm39)	V85L	probably benign	Het
Oxtr	C	T	6: 112,454,138 (GRCm39)	R42Q	probably benign	Het
Parp4	T	C	14: 56,885,576 (GRCm39)	F1552L	unknown	Het
Pigw	G	A	11: 84,769,033 (GRCm39)	H99Y	probably benign	Het
Ppp6r2	G	A	15: 89,158,379 (GRCm39)	M444I	possibly damaging	Het
Rchy1	A	G	5: 92,105,476 (GRCm39)	F82L	probably damaging	Het
Reln	A	C	5: 22,432,626 (GRCm39)	F125V	probably damaging	Het
Riok1	G	A	13: 38,241,149 (GRCm39)	E435K	probably damaging	Het
Rnf213	A	G	11: 119,343,407 (GRCm39)	R3590G	probably damaging	Het
Scart2	A	G	7: 139,874,704 (GRCm39)	E394G	probably damaging	Het
Scrib	G	C	15: 75,923,310 (GRCm39)	P1249A	probably benign	Het
Shc4	C	T	2: 125,520,607 (GRCm39)		probably null	Het
Slc12a2	T	A	18: 58,037,450 (GRCm39)	I520N	probably damaging	Het
Slc26a5	A	G	5: 22,021,959 (GRCm39)	I484T	probably benign	Het
Slc39a11	G	T	11: 113,354,899 (GRCm39)	T110K	probably benign	Het
Snd1	T	A	6: 28,884,970 (GRCm39)	S774T	probably benign	Het
Spata31d1c	C	T	13: 65,184,129 (GRCm39)	P557L	probably damaging	Het
Tada1	A	G	1: 166,216,198 (GRCm39)	D133G	probably benign	Het
Thra	A	G	11: 98,644,455 (GRCm39)	E15G	possibly damaging	Het
Timd5	A	T	11: 46,427,247 (GRCm39)	R187*	probably null	Het
Ttn	T	A	2: 76,625,440 (GRCm39)	I15128F	probably damaging	Het
Ube2r2	A	G	4: 41,174,066 (GRCm39)	Y68C	probably damaging	Het
Uncx	A	T	5: 139,532,442 (GRCm39)	N169I	probably damaging	Het
Vmn2r100	A	G	17: 19,743,786 (GRCm39)	Y483C	possibly damaging	Het
Xkr4	T	C	1: 3,286,897 (GRCm39)	D431G	probably damaging	Het
Xylb	C	A	9: 119,209,091 (GRCm39)	A311E	possibly damaging	Het
Zbtb39	C	G	10: 127,578,175 (GRCm39)	Q250E	probably benign	Het
Zbtb39	C	A	10: 127,578,931 (GRCm39)	H502N	probably damaging	Het
Zfp82	A	G	7: 29,755,876 (GRCm39)	L402P	probably damaging	Het
Zpbp2	G	A	11: 98,448,451 (GRCm39)	R256Q	probably benign	Het

Other mutations in Tg

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00157:Tg	APN	15	66,719,015 (GRCm39)	missense	probably damaging	1.00
IGL00230:Tg	APN	15	66,699,139 (GRCm39)	missense	probably benign	0.00
IGL00324:Tg	APN	15	66,565,273 (GRCm39)	missense	probably benign
IGL00428:Tg	APN	15	66,645,273 (GRCm39)	missense	probably benign	0.33
IGL00703:Tg	APN	15	66,568,338 (GRCm39)	missense	probably benign	0.34
IGL00808:Tg	APN	15	66,555,662 (GRCm39)	missense	probably damaging	1.00
IGL00833:Tg	APN	15	66,560,650 (GRCm39)	missense	probably benign	0.34
IGL00899:Tg	APN	15	66,545,922 (GRCm39)	critical splice donor site	probably null
IGL00921:Tg	APN	15	66,636,302 (GRCm39)	missense	probably benign	0.28
IGL00975:Tg	APN	15	66,553,731 (GRCm39)	missense	probably benign
IGL01288:Tg	APN	15	66,608,125 (GRCm39)	missense	possibly damaging	0.81
IGL01397:Tg	APN	15	66,567,941 (GRCm39)	splice site	probably benign
IGL01634:Tg	APN	15	66,601,415 (GRCm39)	missense	probably benign	0.34
IGL01646:Tg	APN	15	66,549,936 (GRCm39)	missense	probably damaging	1.00
IGL01704:Tg	APN	15	66,543,200 (GRCm39)	missense	probably damaging	0.98
IGL01958:Tg	APN	15	66,631,335 (GRCm39)	missense	probably benign	0.06
IGL02093:Tg	APN	15	66,564,223 (GRCm39)	missense	possibly damaging	0.83
IGL02113:Tg	APN	15	66,577,179 (GRCm39)	missense	probably benign	0.08
IGL02138:Tg	APN	15	66,589,082 (GRCm39)	missense	probably benign	0.01
IGL02156:Tg	APN	15	66,577,197 (GRCm39)	missense	probably benign	0.19
IGL02169:Tg	APN	15	66,629,792 (GRCm39)	missense	probably benign	0.04
IGL02342:Tg	APN	15	66,636,140 (GRCm39)	missense	probably benign
IGL02434:Tg	APN	15	66,636,191 (GRCm39)	missense	probably damaging	0.97
IGL02506:Tg	APN	15	66,613,443 (GRCm39)	missense	possibly damaging	0.71
IGL02513:Tg	APN	15	66,577,123 (GRCm39)	missense	probably benign
IGL02549:Tg	APN	15	66,711,210 (GRCm39)	missense	probably damaging	1.00
IGL02669:Tg	APN	15	66,620,575 (GRCm39)	splice site	probably benign
IGL02756:Tg	APN	15	66,606,435 (GRCm39)	missense	probably benign
IGL02800:Tg	APN	15	66,629,735 (GRCm39)	missense	probably damaging	1.00
IGL02828:Tg	APN	15	66,554,243 (GRCm39)	missense	probably damaging	1.00
IGL02927:Tg	APN	15	66,549,942 (GRCm39)	missense	probably damaging	1.00
IGL03061:Tg	APN	15	66,543,254 (GRCm39)	missense	probably damaging	1.00
IGL03105:Tg	APN	15	66,586,955 (GRCm39)	missense	probably benign	0.01
IGL03160:Tg	APN	15	66,711,152 (GRCm39)	nonsense	probably null
IGL03242:Tg	APN	15	66,555,647 (GRCm39)	missense	probably damaging	0.99
Also_ran	UTSW	15	66,550,688 (GRCm39)	missense	probably damaging	1.00
bedraggled	UTSW	15	66,612,563 (GRCm39)	missense	probably damaging	1.00
foster	UTSW	15	66,565,109 (GRCm39)	nonsense	probably null
hognose	UTSW	15	66,589,057 (GRCm39)	missense	probably damaging	0.99
ito	UTSW	15	66,638,011 (GRCm39)	nonsense	probably null
ito2	UTSW	15	66,543,245 (GRCm39)	missense	probably damaging	1.00
ito3	UTSW	15	66,645,323 (GRCm39)	missense	probably damaging	1.00
ito4	UTSW	15	66,568,369 (GRCm39)	missense	possibly damaging	0.47
Papua	UTSW	15	66,545,899 (GRCm39)	missense	probably damaging	1.00
Pipistrella	UTSW	15	66,567,984 (GRCm39)	missense	probably damaging	1.00
pluribus	UTSW	15	66,587,012 (GRCm39)	missense	probably damaging	0.98
samarai	UTSW	15	66,629,855 (GRCm39)	critical splice donor site	probably null
sariba	UTSW	15	66,566,719 (GRCm39)	missense	probably benign	0.01
ticker	UTSW	15	66,699,231 (GRCm39)	nonsense	probably null
Vampire	UTSW	15	66,554,676 (GRCm39)	missense	probably damaging	1.00
IGL03134:Tg	UTSW	15	66,612,567 (GRCm39)	missense	probably damaging	1.00
P0019:Tg	UTSW	15	66,560,712 (GRCm39)	missense	probably benign	0.01
R0121:Tg	UTSW	15	66,612,630 (GRCm39)	missense	probably benign	0.04
R0135:Tg	UTSW	15	66,566,719 (GRCm39)	missense	probably benign	0.01
R0227:Tg	UTSW	15	66,570,295 (GRCm39)	missense	possibly damaging	0.84
R0448:Tg	UTSW	15	66,636,291 (GRCm39)	missense	probably damaging	1.00
R0453:Tg	UTSW	15	66,700,382 (GRCm39)	missense	probably benign	0.09
R0504:Tg	UTSW	15	66,554,253 (GRCm39)	missense	probably damaging	0.97
R0543:Tg	UTSW	15	66,601,446 (GRCm39)	missense	probably benign	0.13
R0638:Tg	UTSW	15	66,589,057 (GRCm39)	missense	probably damaging	0.99
R0639:Tg	UTSW	15	66,613,333 (GRCm39)	critical splice acceptor site	probably null
R0646:Tg	UTSW	15	66,601,475 (GRCm39)	missense	probably damaging	0.99
R0666:Tg	UTSW	15	66,609,370 (GRCm39)	missense	probably benign
R0673:Tg	UTSW	15	66,613,333 (GRCm39)	critical splice acceptor site	probably null
R0689:Tg	UTSW	15	66,711,253 (GRCm39)	splice site	probably benign
R0704:Tg	UTSW	15	66,629,729 (GRCm39)	missense	probably benign	0.02
R0730:Tg	UTSW	15	66,550,638 (GRCm39)	missense	probably damaging	1.00
R0830:Tg	UTSW	15	66,596,993 (GRCm39)	missense	probably damaging	1.00
R1027:Tg	UTSW	15	66,544,258 (GRCm39)	missense	possibly damaging	0.65
R1061:Tg	UTSW	15	66,570,408 (GRCm39)	missense	probably benign	0.09
R1086:Tg	UTSW	15	66,555,911 (GRCm39)	missense	probably benign
R1103:Tg	UTSW	15	66,591,504 (GRCm39)	missense	probably benign	0.45
R1240:Tg	UTSW	15	66,700,397 (GRCm39)	missense	probably benign	0.16
R1281:Tg	UTSW	15	66,568,338 (GRCm39)	missense	probably benign	0.34
R1470:Tg	UTSW	15	66,721,312 (GRCm39)	missense	possibly damaging	0.95
R1470:Tg	UTSW	15	66,721,312 (GRCm39)	missense	possibly damaging	0.95
R1531:Tg	UTSW	15	66,722,351 (GRCm39)	missense	probably benign	0.02
R1544:Tg	UTSW	15	66,577,081 (GRCm39)	missense	probably benign	0.04
R1550:Tg	UTSW	15	66,565,279 (GRCm39)	missense	possibly damaging	0.52
R1575:Tg	UTSW	15	66,601,534 (GRCm39)	critical splice donor site	probably null
R1638:Tg	UTSW	15	66,568,015 (GRCm39)	nonsense	probably null
R1655:Tg	UTSW	15	66,700,417 (GRCm39)	critical splice donor site	probably null
R1671:Tg	UTSW	15	66,564,236 (GRCm39)	missense	possibly damaging	0.89
R1789:Tg	UTSW	15	66,609,397 (GRCm39)	missense	probably benign	0.00
R1883:Tg	UTSW	15	66,543,158 (GRCm39)	missense	probably damaging	1.00
R1984:Tg	UTSW	15	66,554,691 (GRCm39)	missense	probably benign
R2063:Tg	UTSW	15	66,700,402 (GRCm39)	missense	probably damaging	1.00
R2092:Tg	UTSW	15	66,721,456 (GRCm39)	missense	probably null	0.26
R2109:Tg	UTSW	15	66,601,443 (GRCm39)	missense	probably benign	0.02
R2128:Tg	UTSW	15	66,566,743 (GRCm39)	missense	probably benign	0.10
R2129:Tg	UTSW	15	66,566,743 (GRCm39)	missense	probably benign	0.10
R2207:Tg	UTSW	15	66,553,788 (GRCm39)	missense	probably benign	0.15
R2219:Tg	UTSW	15	66,553,782 (GRCm39)	missense	probably benign	0.03
R2228:Tg	UTSW	15	66,545,860 (GRCm39)	missense	probably damaging	0.99
R2229:Tg	UTSW	15	66,545,860 (GRCm39)	missense	probably damaging	0.99
R2259:Tg	UTSW	15	66,555,747 (GRCm39)	missense	probably benign
R2994:Tg	UTSW	15	66,553,802 (GRCm39)	missense	probably benign
R3904:Tg	UTSW	15	66,638,011 (GRCm39)	nonsense	probably null
R3946:Tg	UTSW	15	66,545,872 (GRCm39)	missense	probably damaging	1.00
R3965:Tg	UTSW	15	66,556,039 (GRCm39)	missense	probably benign
R4245:Tg	UTSW	15	66,568,318 (GRCm39)	missense	possibly damaging	0.68
R4451:Tg	UTSW	15	66,637,996 (GRCm39)	missense	probably benign	0.01
R4487:Tg	UTSW	15	66,543,245 (GRCm39)	missense	probably damaging	1.00
R4489:Tg	UTSW	15	66,579,791 (GRCm39)	missense	probably damaging	1.00
R4623:Tg	UTSW	15	66,607,120 (GRCm39)	missense	probably benign	0.23
R4659:Tg	UTSW	15	66,545,769 (GRCm39)	missense	possibly damaging	0.67
R4728:Tg	UTSW	15	66,554,676 (GRCm39)	missense	probably damaging	1.00
R4760:Tg	UTSW	15	66,565,168 (GRCm39)	missense	probably damaging	1.00
R4797:Tg	UTSW	15	66,629,855 (GRCm39)	critical splice donor site	probably null
R4944:Tg	UTSW	15	66,636,186 (GRCm39)	missense	probably damaging	1.00
R4998:Tg	UTSW	15	66,545,899 (GRCm39)	missense	probably damaging	1.00
R5009:Tg	UTSW	15	66,568,435 (GRCm39)	missense	probably benign	0.01
R5025:Tg	UTSW	15	66,579,779 (GRCm39)	missense	probably damaging	1.00
R5035:Tg	UTSW	15	66,553,662 (GRCm39)	splice site	probably null
R5049:Tg	UTSW	15	66,699,231 (GRCm39)	nonsense	probably null
R5073:Tg	UTSW	15	66,607,101 (GRCm39)	missense	probably benign	0.05
R5169:Tg	UTSW	15	66,550,629 (GRCm39)	nonsense	probably null
R5185:Tg	UTSW	15	66,645,323 (GRCm39)	missense	probably damaging	1.00
R5227:Tg	UTSW	15	66,631,416 (GRCm39)	missense	possibly damaging	0.87
R5300:Tg	UTSW	15	66,550,704 (GRCm39)	missense	probably damaging	1.00
R5334:Tg	UTSW	15	66,549,904 (GRCm39)	missense	probably damaging	1.00
R5339:Tg	UTSW	15	66,549,942 (GRCm39)	missense	probably damaging	1.00
R5402:Tg	UTSW	15	66,611,017 (GRCm39)	missense	probably damaging	0.98
R5441:Tg	UTSW	15	66,568,369 (GRCm39)	missense	possibly damaging	0.47
R5509:Tg	UTSW	15	66,699,142 (GRCm39)	missense	probably benign	0.45
R5580:Tg	UTSW	15	66,557,149 (GRCm39)	missense	possibly damaging	0.66
R5582:Tg	UTSW	15	66,565,284 (GRCm39)	missense	probably damaging	1.00
R5624:Tg	UTSW	15	66,709,906 (GRCm39)	missense	probably benign	0.11
R5686:Tg	UTSW	15	66,560,738 (GRCm39)	missense	probably benign	0.28
R6042:Tg	UTSW	15	66,555,842 (GRCm39)	missense	probably benign	0.01
R6122:Tg	UTSW	15	66,700,306 (GRCm39)	missense	probably damaging	1.00
R6146:Tg	UTSW	15	66,545,216 (GRCm39)	splice site	probably null
R6159:Tg	UTSW	15	66,607,096 (GRCm39)	missense	possibly damaging	0.71
R6223:Tg	UTSW	15	66,579,771 (GRCm39)	missense	probably benign	0.15
R6480:Tg	UTSW	15	66,543,160 (GRCm39)	missense	probably damaging	1.00
R6505:Tg	UTSW	15	66,631,407 (GRCm39)	missense	probably damaging	0.99
R6531:Tg	UTSW	15	66,711,211 (GRCm39)	missense	probably damaging	0.99
R6614:Tg	UTSW	15	66,607,108 (GRCm39)	missense	probably damaging	0.99
R6698:Tg	UTSW	15	66,711,211 (GRCm39)	missense	probably damaging	1.00
R6798:Tg	UTSW	15	66,550,688 (GRCm39)	missense	probably damaging	1.00
R6837:Tg	UTSW	15	66,567,984 (GRCm39)	missense	probably damaging	1.00
R6861:Tg	UTSW	15	66,560,740 (GRCm39)	missense	probably benign	0.00
R6888:Tg	UTSW	15	66,568,095 (GRCm39)	missense	probably damaging	0.99
R6933:Tg	UTSW	15	66,636,158 (GRCm39)	missense	possibly damaging	0.73
R6983:Tg	UTSW	15	66,565,207 (GRCm39)	missense	probably benign	0.01
R7078:Tg	UTSW	15	66,545,392 (GRCm39)	missense	probably damaging	1.00
R7244:Tg	UTSW	15	66,612,563 (GRCm39)	missense	probably damaging	1.00
R7320:Tg	UTSW	15	66,566,633 (GRCm39)	missense	possibly damaging	0.71
R7334:Tg	UTSW	15	66,597,121 (GRCm39)	missense	probably benign	0.01
R7418:Tg	UTSW	15	66,568,432 (GRCm39)	missense	probably damaging	0.99
R7485:Tg	UTSW	15	66,568,437 (GRCm39)	missense	probably benign	0.04
R7524:Tg	UTSW	15	66,568,010 (GRCm39)	missense	probably benign	0.01
R7529:Tg	UTSW	15	66,566,617 (GRCm39)	missense	probably damaging	0.99
R7540:Tg	UTSW	15	66,561,776 (GRCm39)	missense	probably benign	0.16
R7583:Tg	UTSW	15	66,636,267 (GRCm39)	missense	probably damaging	1.00
R7594:Tg	UTSW	15	66,601,432 (GRCm39)	missense	probably benign	0.20
R7667:Tg	UTSW	15	66,587,012 (GRCm39)	missense	probably damaging	0.98
R7722:Tg	UTSW	15	66,636,158 (GRCm39)	missense	possibly damaging	0.73
R7790:Tg	UTSW	15	66,721,453 (GRCm39)	missense	probably damaging	0.99
R7838:Tg	UTSW	15	66,565,112 (GRCm39)	missense	probably benign	0.00
R7890:Tg	UTSW	15	66,555,663 (GRCm39)	missense	probably damaging	1.00
R7904:Tg	UTSW	15	66,577,128 (GRCm39)	missense	probably benign	0.08
R7919:Tg	UTSW	15	66,555,923 (GRCm39)	missense	possibly damaging	0.73
R7921:Tg	UTSW	15	66,555,642 (GRCm39)	missense	probably benign	0.08
R8037:Tg	UTSW	15	66,560,724 (GRCm39)	missense	probably benign	0.00
R8038:Tg	UTSW	15	66,560,724 (GRCm39)	missense	probably benign	0.00
R8214:Tg	UTSW	15	66,645,247 (GRCm39)	missense	probably damaging	1.00
R8304:Tg	UTSW	15	66,565,109 (GRCm39)	nonsense	probably null
R8688:Tg	UTSW	15	66,566,802 (GRCm39)	critical splice donor site	probably benign
R8709:Tg	UTSW	15	66,553,786 (GRCm39)	missense	probably benign	0.08
R8714:Tg	UTSW	15	66,555,891 (GRCm39)	missense	probably damaging	0.97
R8901:Tg	UTSW	15	66,557,184 (GRCm39)	missense	probably damaging	1.00
R8917:Tg	UTSW	15	66,645,332 (GRCm39)	critical splice donor site	probably null
R9023:Tg	UTSW	15	66,555,522 (GRCm39)	missense	probably damaging	1.00
R9232:Tg	UTSW	15	66,570,310 (GRCm39)	missense	probably benign	0.01
R9310:Tg	UTSW	15	66,699,118 (GRCm39)	missense	possibly damaging	0.69
R9361:Tg	UTSW	15	66,557,246 (GRCm39)	missense	possibly damaging	0.50
R9389:Tg	UTSW	15	66,561,173 (GRCm39)	missense	probably benign	0.04
R9501:Tg	UTSW	15	66,718,923 (GRCm39)	missense	possibly damaging	0.52
R9510:Tg	UTSW	15	66,545,913 (GRCm39)	missense	probably damaging	1.00
R9594:Tg	UTSW	15	66,607,109 (GRCm39)	nonsense	probably null
R9629:Tg	UTSW	15	66,555,587 (GRCm39)	missense	possibly damaging	0.95
R9701:Tg	UTSW	15	66,637,991 (GRCm39)	missense	probably benign	0.03
R9743:Tg	UTSW	15	66,561,839 (GRCm39)	missense	probably benign	0.18
R9748:Tg	UTSW	15	66,719,008 (GRCm39)	missense	possibly damaging	0.91
T0975:Tg	UTSW	15	66,560,712 (GRCm39)	missense	probably benign	0.01
X0005:Tg	UTSW	15	66,560,712 (GRCm39)	missense	probably benign	0.01
X0065:Tg	UTSW	15	66,554,303 (GRCm39)	missense	probably damaging	1.00
X0067:Tg	UTSW	15	66,620,592 (GRCm39)	missense	probably benign	0.10
Z1177:Tg	UTSW	15	66,721,396 (GRCm39)	missense	probably benign	0.02
Z1177:Tg	UTSW	15	66,557,159 (GRCm39)	missense	possibly damaging	0.49

Predicted Primers

PCR Primer
(F):5'- GCTGGAGGTGGTTACACTACAAGC -3'
(R):5'- TGCTACCCAGACTAACAGAGGCAAG -3'

Sequencing Primer
(F):5'- AGCCAGAATGTCTGTGTTACC -3'
(R):5'- GCCTCAGCAGGTCAACTTTC -3'

Posted On

2013-11-08