Incidental Mutation 'R0960:Tmem25'
| ID |
81831 |
| Institutional Source |
Beutler Lab
|
| Gene Symbol |
Tmem25
|
| Ensembl Gene |
ENSMUSG00000002032 |
| Gene Name |
transmembrane protein 25 |
| Synonyms |
0610039J01Rik |
| MMRRC Submission |
039089-MU
|
| Accession Numbers |
|
| Essential gene? |
Probably non essential
(E-score: 0.061)
|
| Stock # |
R0960 (G1)
|
| Quality Score |
225 |
|
Status
|
Validated
|
| Chromosome |
9 |
| Chromosomal Location |
44705066-44710604 bp(-) (GRCm39) |
| Type of Mutation |
critical splice acceptor site |
| DNA Base Change (assembly) |
T to A
at 44706809 bp (GRCm39)
|
| Zygosity |
Heterozygous |
| Amino Acid Change |
|
| Ref Sequence |
ENSEMBL: ENSMUSP00000110353
(fasta)
|
| Gene Model |
predicted gene model for transcript(s):
[ENSMUST00000002099]
[ENSMUST00000002100]
[ENSMUST00000002100]
[ENSMUST00000044694]
[ENSMUST00000114705]
[ENSMUST00000114705]
[ENSMUST00000114705]
[ENSMUST00000114705]
[ENSMUST00000118186]
[ENSMUST00000132020]
[ENSMUST00000213972]
[ENSMUST00000213203]
[ENSMUST00000214833]
[ENSMUST00000214431]
[ENSMUST00000213363]
|
| AlphaFold |
Q9DCF1 |
| Predicted Effect |
probably benign
Transcript: ENSMUST00000002099
|
| SMART Domains |
Protein: ENSMUSP00000002099
Gene: ENSMUSG00000002031
| Domain | Start | End | E-Value | Type |
|---|
|
low complexity region
|
3 |
18 |
N/A |
INTRINSIC |
|
low complexity region
|
29 |
52 |
N/A |
INTRINSIC |
|
Pfam:IFT46_B_C
|
60 |
270 |
6.2e-106 |
PFAM |
|
| Predicted Effect |
probably null
Transcript: ENSMUST00000002100
|
| SMART Domains |
Protein: ENSMUSP00000002100
Gene: ENSMUSG00000002032
| Domain | Start | End | E-Value | Type |
|---|
|
low complexity region
|
5 |
23 |
N/A |
INTRINSIC |
|
Pfam:C2-set_2
|
26 |
118 |
2.7e-14 |
PFAM |
|
SCOP:d1ie5a_
|
121 |
220 |
2e-6 |
SMART |
|
transmembrane domain
|
238 |
260 |
N/A |
INTRINSIC |
|
low complexity region
|
327 |
338 |
N/A |
INTRINSIC |
|
| Predicted Effect |
probably null
Transcript: ENSMUST00000002100
|
| SMART Domains |
Protein: ENSMUSP00000002100
Gene: ENSMUSG00000002032
| Domain | Start | End | E-Value | Type |
|---|
|
low complexity region
|
5 |
23 |
N/A |
INTRINSIC |
|
Pfam:C2-set_2
|
26 |
118 |
2.7e-14 |
PFAM |
|
SCOP:d1ie5a_
|
121 |
220 |
2e-6 |
SMART |
|
transmembrane domain
|
238 |
260 |
N/A |
INTRINSIC |
|
low complexity region
|
327 |
338 |
N/A |
INTRINSIC |
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000044694
|
| SMART Domains |
Protein: ENSMUSP00000042183
Gene: ENSMUSG00000039438
| Domain | Start | End | E-Value | Type |
|---|
|
low complexity region
|
22 |
39 |
N/A |
INTRINSIC |
|
Pfam:TPR_11
|
46 |
112 |
2.1e-13 |
PFAM |
|
Pfam:TPR_9
|
54 |
123 |
5.8e-7 |
PFAM |
|
Pfam:TPR_1
|
82 |
113 |
2.3e-6 |
PFAM |
|
Pfam:TPR_2
|
82 |
114 |
1.2e-5 |
PFAM |
|
| Predicted Effect |
probably null
Transcript: ENSMUST00000114705
|
| SMART Domains |
Protein: ENSMUSP00000110353
Gene: ENSMUSG00000002032
| Domain | Start | End | E-Value | Type |
|---|
|
low complexity region
|
5 |
23 |
N/A |
INTRINSIC |
|
Pfam:C2-set_2
|
26 |
118 |
2.2e-15 |
PFAM |
|
SCOP:d1ie5a_
|
121 |
220 |
2e-6 |
SMART |
|
transmembrane domain
|
238 |
260 |
N/A |
INTRINSIC |
|
low complexity region
|
327 |
338 |
N/A |
INTRINSIC |
|
| Predicted Effect |
probably null
Transcript: ENSMUST00000114705
|
| SMART Domains |
Protein: ENSMUSP00000110353
Gene: ENSMUSG00000002032
| Domain | Start | End | E-Value | Type |
|---|
|
low complexity region
|
5 |
23 |
N/A |
INTRINSIC |
|
Pfam:C2-set_2
|
26 |
118 |
2.2e-15 |
PFAM |
|
SCOP:d1ie5a_
|
121 |
220 |
2e-6 |
SMART |
|
transmembrane domain
|
238 |
260 |
N/A |
INTRINSIC |
|
low complexity region
|
327 |
338 |
N/A |
INTRINSIC |
|
| Predicted Effect |
probably null
Transcript: ENSMUST00000114705
|
| SMART Domains |
Protein: ENSMUSP00000110353
Gene: ENSMUSG00000002032
| Domain | Start | End | E-Value | Type |
|---|
|
low complexity region
|
5 |
23 |
N/A |
INTRINSIC |
|
Pfam:C2-set_2
|
26 |
118 |
2.2e-15 |
PFAM |
|
SCOP:d1ie5a_
|
121 |
220 |
2e-6 |
SMART |
|
transmembrane domain
|
238 |
260 |
N/A |
INTRINSIC |
|
low complexity region
|
327 |
338 |
N/A |
INTRINSIC |
|
| Predicted Effect |
probably null
Transcript: ENSMUST00000114705
|
| SMART Domains |
Protein: ENSMUSP00000110353
Gene: ENSMUSG00000002032
| Domain | Start | End | E-Value | Type |
|---|
|
low complexity region
|
5 |
23 |
N/A |
INTRINSIC |
|
Pfam:C2-set_2
|
26 |
118 |
2.2e-15 |
PFAM |
|
SCOP:d1ie5a_
|
121 |
220 |
2e-6 |
SMART |
|
transmembrane domain
|
238 |
260 |
N/A |
INTRINSIC |
|
low complexity region
|
327 |
338 |
N/A |
INTRINSIC |
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000118186
|
| SMART Domains |
Protein: ENSMUSP00000113845
Gene: ENSMUSG00000002031
| Domain | Start | End | E-Value | Type |
|---|
|
low complexity region
|
3 |
18 |
N/A |
INTRINSIC |
|
low complexity region
|
29 |
52 |
N/A |
INTRINSIC |
|
Pfam:IFT46_B_C
|
59 |
272 |
1.1e-105 |
PFAM |
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000132020
|
| Predicted Effect |
noncoding transcript
Transcript: ENSMUST00000149020
|
| Predicted Effect |
noncoding transcript
Transcript: ENSMUST00000216449
|
| Predicted Effect |
noncoding transcript
Transcript: ENSMUST00000214931
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000213972
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000213203
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000214833
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000214431
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000213363
|
| Meta Mutation Damage Score |
0.9501 |
| Coding Region Coverage |
- 1x: 99.5%
- 3x: 99.0%
- 10x: 97.8%
- 20x: 96.0%
|
| Validation Efficiency |
98% (49/50) |
| Allele List at MGI |
|
| Other mutations in this stock |
Total: 48 list
| Gene | Ref | Var | Chr/Loc | Mutation | Predicted Effect | Zygosity |
|---|
| 1810009A15Rik |
T |
C |
19: 8,867,792 (GRCm39) |
V256A |
probably benign |
Het |
| 4930433I11Rik |
A |
T |
7: 40,642,480 (GRCm39) |
T141S |
probably benign |
Het |
| 4930474N05Rik |
C |
A |
14: 35,818,367 (GRCm39) |
H122N |
probably benign |
Het |
| Aamp |
T |
C |
1: 74,320,304 (GRCm39) |
T341A |
possibly damaging |
Het |
| Adam26a |
A |
T |
8: 44,021,800 (GRCm39) |
H563Q |
probably damaging |
Het |
| Ankrd13a |
G |
A |
5: 114,924,868 (GRCm39) |
E118K |
probably benign |
Het |
| Asic5 |
A |
G |
3: 81,913,847 (GRCm39) |
I174V |
probably benign |
Het |
| Atad2b |
G |
A |
12: 5,056,593 (GRCm39) |
|
probably benign |
Het |
| Bub1b |
A |
G |
2: 118,437,161 (GRCm39) |
I120V |
probably benign |
Het |
| Casp8 |
T |
C |
1: 58,868,172 (GRCm39) |
|
probably null |
Het |
| Cdk5rap2 |
A |
G |
4: 70,161,745 (GRCm39) |
Y254H |
probably benign |
Het |
| Clasp1 |
T |
C |
1: 118,479,756 (GRCm39) |
I996T |
probably benign |
Het |
| Cntn6 |
A |
G |
6: 104,751,441 (GRCm39) |
I294V |
probably benign |
Het |
| Flnc |
A |
G |
6: 29,441,511 (GRCm39) |
D431G |
probably damaging |
Het |
| Gm4884 |
T |
A |
7: 40,692,232 (GRCm39) |
M67K |
possibly damaging |
Het |
| Hmx3 |
T |
C |
7: 131,145,043 (GRCm39) |
Y118H |
probably benign |
Het |
| Hsf2bp |
C |
T |
17: 32,226,743 (GRCm39) |
R204H |
probably damaging |
Het |
| Il12b |
G |
T |
11: 44,299,315 (GRCm39) |
C128F |
probably damaging |
Het |
| Ints6 |
T |
C |
14: 62,947,015 (GRCm39) |
M317V |
probably benign |
Het |
| Iqca1 |
C |
A |
1: 90,070,453 (GRCm39) |
G133V |
probably null |
Het |
| Kcnh2 |
C |
T |
5: 24,527,670 (GRCm39) |
R894H |
probably damaging |
Het |
| Kif27 |
T |
C |
13: 58,471,781 (GRCm39) |
E769G |
probably damaging |
Het |
| Kif28 |
G |
A |
1: 179,523,370 (GRCm39) |
Q987* |
probably null |
Het |
| Klhdc3 |
T |
C |
17: 46,987,444 (GRCm39) |
H330R |
possibly damaging |
Het |
| Leo1 |
G |
A |
9: 75,352,522 (GRCm39) |
E22K |
probably benign |
Het |
| Lpcat2 |
C |
T |
8: 93,596,338 (GRCm39) |
T125M |
probably benign |
Het |
| Map1a |
A |
G |
2: 121,132,124 (GRCm39) |
Y742C |
probably benign |
Het |
| Mllt6 |
C |
T |
11: 97,555,772 (GRCm39) |
|
probably benign |
Het |
| Mpp2 |
A |
G |
11: 101,952,411 (GRCm39) |
V354A |
possibly damaging |
Het |
| Mroh2a |
C |
A |
1: 88,170,142 (GRCm39) |
A685D |
possibly damaging |
Het |
| Myo10 |
A |
G |
15: 25,801,275 (GRCm39) |
E1488G |
probably damaging |
Het |
| Neb |
A |
G |
2: 52,102,995 (GRCm39) |
V4461A |
probably benign |
Het |
| Nudcd1 |
G |
T |
15: 44,291,047 (GRCm39) |
|
probably benign |
Het |
| Or10ag59 |
T |
C |
2: 87,406,279 (GRCm39) |
Y284H |
probably benign |
Het |
| Or2w1b |
A |
C |
13: 21,300,435 (GRCm39) |
D191A |
possibly damaging |
Het |
| Pde1a |
G |
A |
2: 79,695,378 (GRCm39) |
|
probably benign |
Het |
| Sdha |
A |
T |
13: 74,471,303 (GRCm39) |
|
probably benign |
Het |
| Selenoo |
T |
G |
15: 88,980,957 (GRCm39) |
I432S |
probably benign |
Het |
| Sh3gl2 |
A |
T |
4: 85,295,717 (GRCm39) |
I140F |
probably damaging |
Het |
| Svopl |
G |
T |
6: 37,993,992 (GRCm39) |
Y346* |
probably null |
Het |
| Tbc1d17 |
C |
T |
7: 44,497,852 (GRCm39) |
|
probably benign |
Het |
| Tlr3 |
A |
C |
8: 45,850,452 (GRCm39) |
I815S |
probably damaging |
Het |
| Tpd52 |
A |
T |
3: 9,008,650 (GRCm39) |
|
probably null |
Het |
| Tspoap1 |
A |
T |
11: 87,661,421 (GRCm39) |
|
probably benign |
Het |
| Txndc11 |
A |
T |
16: 10,909,453 (GRCm39) |
D364E |
probably benign |
Het |
| Unc5cl |
G |
T |
17: 48,766,624 (GRCm39) |
|
probably benign |
Het |
| Vmn1r13 |
A |
G |
6: 57,186,996 (GRCm39) |
M52V |
probably benign |
Het |
| Zap70 |
T |
C |
1: 36,818,254 (GRCm39) |
Y314H |
probably damaging |
Het |
|
| Other mutations in Tmem25 |
| Allele | Source | Chr | Coord | Type | Predicted Effect | PPH Score |
|---|
|
IGL00596:Tmem25
|
APN |
9 |
44,706,816 (GRCm39) |
unclassified |
probably benign |
|
|
IGL01980:Tmem25
|
APN |
9 |
44,709,568 (GRCm39) |
nonsense |
probably null |
|
|
IGL02000:Tmem25
|
APN |
9 |
44,709,568 (GRCm39) |
nonsense |
probably null |
|
|
IGL03407:Tmem25
|
APN |
9 |
44,707,636 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R0446:Tmem25
|
UTSW |
9 |
44,707,878 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R0690:Tmem25
|
UTSW |
9 |
44,706,811 (GRCm39) |
unclassified |
probably benign |
|
|
R1204:Tmem25
|
UTSW |
9 |
44,706,529 (GRCm39) |
missense |
probably benign |
0.17 |
|
R1899:Tmem25
|
UTSW |
9 |
44,709,513 (GRCm39) |
splice site |
probably null |
|
|
R1927:Tmem25
|
UTSW |
9 |
44,707,780 (GRCm39) |
missense |
possibly damaging |
0.77 |
|
R2088:Tmem25
|
UTSW |
9 |
44,707,383 (GRCm39) |
missense |
possibly damaging |
0.94 |
|
R3027:Tmem25
|
UTSW |
9 |
44,709,511 (GRCm39) |
splice site |
probably null |
|
|
R6854:Tmem25
|
UTSW |
9 |
44,707,305 (GRCm39) |
missense |
possibly damaging |
0.46 |
|
R7305:Tmem25
|
UTSW |
9 |
44,706,705 (GRCm39) |
critical splice donor site |
probably null |
|
|
R7655:Tmem25
|
UTSW |
9 |
44,709,640 (GRCm39) |
missense |
possibly damaging |
0.82 |
|
R7656:Tmem25
|
UTSW |
9 |
44,709,640 (GRCm39) |
missense |
possibly damaging |
0.82 |
|
R7951:Tmem25
|
UTSW |
9 |
44,706,790 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R7968:Tmem25
|
UTSW |
9 |
44,706,983 (GRCm39) |
missense |
probably benign |
0.00 |
|
R8298:Tmem25
|
UTSW |
9 |
44,710,116 (GRCm39) |
start gained |
probably benign |
|
|
R9177:Tmem25
|
UTSW |
9 |
44,709,529 (GRCm39) |
missense |
possibly damaging |
0.95 |
|
R9207:Tmem25
|
UTSW |
9 |
44,710,476 (GRCm39) |
critical splice donor site |
probably null |
|
|
R9274:Tmem25
|
UTSW |
9 |
44,706,336 (GRCm39) |
missense |
probably benign |
0.00 |
|
R9291:Tmem25
|
UTSW |
9 |
44,706,799 (GRCm39) |
missense |
probably damaging |
0.98 |
|
R9645:Tmem25
|
UTSW |
9 |
44,706,515 (GRCm39) |
missense |
|
|
|
| Predicted Primers |
PCR Primer
(F):5'- CACTGGACACACGGTAGATGTAGC -3'
(R):5'- TGACATGGTTTCTTTGTCCTCAGGC -3'
Sequencing Primer
(F):5'- GCCTTGGGTATAACAAGCAC -3'
(R):5'- TTGTCCTCAGGCCCCTC -3'
|
| Posted On |
2013-11-08 |
Incidental Mutation