Mutagenetix > Incidental Mutation

Incidental Mutation 'R0943:Zfp735'

81975

Institutional Source

Beutler Lab

Gene Symbol

Zfp735

Ensembl Gene

ENSMUSG00000060630

Gene Name

zinc finger protein 735

Synonyms

1700012C15Rik

MMRRC Submission

039082-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.069) question?

Stock #

R0943 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

73579604-73604624 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 73602909 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Alanine at position 618 (T618A)

Ref Sequence

ENSEMBL: ENSMUSP00000079269 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000080407]

AlphaFold

B1ARH2

Predicted Effect

probably benign
Transcript: ENSMUST00000080407
AA Change: T618A

PolyPhen 2 Score 0.036 (Sensitivity: 0.94; Specificity: 0.82)

SMART Domains

Protein: ENSMUSP00000079269
Gene: ENSMUSG00000060630
AA Change: T618A

Domain	Start	End	E-Value	Type
KRAB	8	68	2.2e-34	SMART
ZnF_C2H2	483	505	4.38e1	SMART
ZnF_C2H2	511	533	2.67e-1	SMART
ZnF_C2H2	539	561	1.81e1	SMART
ZnF_C2H2	567	589	1.5e-4	SMART
ZnF_C2H2	595	617	4.87e-4	SMART
ZnF_C2H2	623	645	4.24e-4	SMART
ZnF_C2H2	651	673	2.27e-4	SMART
ZnF_C2H2	679	701	7.49e-5	SMART
ZnF_C2H2	707	729	4.87e-4	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000145996

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000149560

Meta Mutation Damage Score

0.2870

Coding Region Coverage

1x: 99.4%
3x: 98.8%
10x: 97.3%
20x: 94.5%

Validation Efficiency

97% (36/37)

Allele List at MGI

Other mutations in this stock

Total: 37 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Agtpbp1	T	C	13: 59,648,416 (GRCm39)	N468S	probably benign	Het
Card6	A	G	15: 5,129,768 (GRCm39)	S543P	probably damaging	Het
Celsr1	T	G	15: 85,787,489 (GRCm39)	T2750P	probably damaging	Het
Csmd3	A	G	15: 47,539,135 (GRCm39)	M2341T	probably damaging	Het
Dym	A	G	18: 75,419,840 (GRCm39)	*670W	probably null	Het
Ehbp1	T	C	11: 22,045,883 (GRCm39)	D597G	probably benign	Het
Emx1	G	A	6: 85,180,901 (GRCm39)	W206*	probably null	Het
Esr1	A	G	10: 4,696,781 (GRCm39)	K210R	probably damaging	Het
Extl1	TGCGTTGCACCGATACCGGG	TG	4: 134,084,988 (GRCm39)		probably benign	Het
Fads2b	C	T	2: 85,319,109 (GRCm39)	D398N	probably damaging	Het
Fam72a	T	C	1: 131,456,517 (GRCm39)	S27P	possibly damaging	Het
Fanca	A	T	8: 124,000,925 (GRCm39)	C1152S	probably damaging	Het
Fras1	G	A	5: 96,874,402 (GRCm39)	V2276I	probably benign	Het
Hoxb13	A	G	11: 96,086,799 (GRCm39)	E202G	probably benign	Het
Lcmt1	T	G	7: 123,000,662 (GRCm39)		probably null	Het
Nars2	C	T	7: 96,605,138 (GRCm39)		probably benign	Het
Neil3	ATATTTATTTATTTATTTATTTATTTATTTATT	ATATTTATTTATTTATTTATTTATTTATTTATTTATT	8: 54,062,404 (GRCm39)		probably benign	Het
Nup153	T	C	13: 46,850,248 (GRCm39)		probably benign	Het
Or4c10b	T	C	2: 89,711,305 (GRCm39)	V45A	probably benign	Het
Or5b112	C	A	19: 13,319,157 (GRCm39)	H12N	probably benign	Het
Prkar2a	T	C	9: 108,610,475 (GRCm39)		probably benign	Het
Ptprc	T	C	1: 138,038,902 (GRCm39)	T209A	probably damaging	Het
Rif1	GCCACCA	GCCA	2: 52,000,336 (GRCm39)		probably benign	Het
Rnf26rt	T	C	6: 76,473,398 (GRCm39)	H406R	probably benign	Het
Rprd2	C	T	3: 95,691,559 (GRCm39)	V239I	possibly damaging	Het
Sgo2b	A	G	8: 64,384,369 (GRCm39)	F209S	possibly damaging	Het
Shfl	A	T	9: 20,784,258 (GRCm39)	H160L	possibly damaging	Het
Spry2	T	C	14: 106,131,021 (GRCm39)	Y55C	probably damaging	Het
Tbc1d32	A	T	10: 56,037,243 (GRCm39)	V667E	probably benign	Het
Tbrg4	A	G	11: 6,569,008 (GRCm39)	F388L	probably damaging	Het
Tmt1a	T	C	15: 100,202,839 (GRCm39)	Y20H	probably benign	Het
Tshz1	T	C	18: 84,033,356 (GRCm39)	T351A	probably benign	Het
Usp48	A	G	4: 137,371,781 (GRCm39)	N969S	possibly damaging	Het
Vmn2r108	A	T	17: 20,691,397 (GRCm39)	C375*	probably null	Het
Vps45	T	A	3: 95,964,336 (GRCm39)	I62F	probably benign	Het
Xab2	A	G	8: 3,663,667 (GRCm39)	F388L	probably benign	Het
Zswim2	T	A	2: 83,748,342 (GRCm39)	R279S	possibly damaging	Het

Other mutations in Zfp735

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00492:Zfp735	APN	11	73,602,192 (GRCm39)	missense	possibly damaging	0.86
IGL00798:Zfp735	APN	11	73,602,386 (GRCm39)	missense	possibly damaging	0.72
IGL01642:Zfp735	APN	11	73,601,305 (GRCm39)	missense	possibly damaging	0.73
IGL01684:Zfp735	APN	11	73,581,191 (GRCm39)	missense	possibly damaging	0.86
IGL02096:Zfp735	APN	11	73,602,254 (GRCm39)	missense	probably benign	0.01
IGL02238:Zfp735	APN	11	73,601,319 (GRCm39)	missense	probably benign	0.00
IGL02505:Zfp735	APN	11	73,580,626 (GRCm39)	missense	probably benign	0.03
IGL02740:Zfp735	APN	11	73,601,412 (GRCm39)	missense	possibly damaging	0.53
IGL02957:Zfp735	APN	11	73,601,755 (GRCm39)	missense	probably benign	0.00
bananaquit	UTSW	11	73,601,412 (GRCm39)	nonsense	probably null
bescher	UTSW	11	73,602,979 (GRCm39)	missense	possibly damaging	0.93
Galvanic	UTSW	11	73,602,504 (GRCm39)	nonsense	probably null
grassquit	UTSW	11	73,603,029 (GRCm39)	missense	possibly damaging	0.66
R0114:Zfp735	UTSW	11	73,601,488 (GRCm39)	missense	probably benign	0.33
R0217:Zfp735	UTSW	11	73,602,112 (GRCm39)	missense	possibly damaging	0.73
R1421:Zfp735	UTSW	11	73,601,523 (GRCm39)	missense	probably benign
R1460:Zfp735	UTSW	11	73,603,159 (GRCm39)	missense	possibly damaging	0.73
R1493:Zfp735	UTSW	11	73,601,305 (GRCm39)	missense	possibly damaging	0.73
R1517:Zfp735	UTSW	11	73,601,470 (GRCm39)	missense	probably benign
R1676:Zfp735	UTSW	11	73,602,301 (GRCm39)	missense	possibly damaging	0.53
R1709:Zfp735	UTSW	11	73,602,589 (GRCm39)	missense	probably benign	0.01
R1871:Zfp735	UTSW	11	73,601,412 (GRCm39)	nonsense	probably null
R1931:Zfp735	UTSW	11	73,602,677 (GRCm39)	missense	possibly damaging	0.69
R2219:Zfp735	UTSW	11	73,601,851 (GRCm39)	missense	possibly damaging	0.53
R2227:Zfp735	UTSW	11	73,602,223 (GRCm39)	nonsense	probably null
R2227:Zfp735	UTSW	11	73,602,222 (GRCm39)	missense	possibly damaging	0.53
R3552:Zfp735	UTSW	11	73,602,067 (GRCm39)	nonsense	probably null
R3856:Zfp735	UTSW	11	73,602,282 (GRCm39)	missense	probably benign	0.01
R3925:Zfp735	UTSW	11	73,601,950 (GRCm39)	missense	probably benign	0.33
R4572:Zfp735	UTSW	11	73,580,611 (GRCm39)	missense	probably benign	0.02
R4585:Zfp735	UTSW	11	73,580,550 (GRCm39)	missense	possibly damaging	0.51
R4586:Zfp735	UTSW	11	73,580,550 (GRCm39)	missense	possibly damaging	0.51
R4619:Zfp735	UTSW	11	73,602,031 (GRCm39)	missense	probably damaging	0.98
R4687:Zfp735	UTSW	11	73,602,682 (GRCm39)	missense	probably damaging	0.98
R4687:Zfp735	UTSW	11	73,602,681 (GRCm39)	missense	probably damaging	0.98
R5435:Zfp735	UTSW	11	73,602,939 (GRCm39)	missense	possibly damaging	0.72
R5489:Zfp735	UTSW	11	73,601,419 (GRCm39)	nonsense	probably null
R5516:Zfp735	UTSW	11	73,601,640 (GRCm39)	missense	probably benign
R5654:Zfp735	UTSW	11	73,602,964 (GRCm39)	missense	possibly damaging	0.71
R5990:Zfp735	UTSW	11	73,581,174 (GRCm39)	missense	possibly damaging	0.70
R6332:Zfp735	UTSW	11	73,602,504 (GRCm39)	nonsense	probably null
R6427:Zfp735	UTSW	11	73,581,140 (GRCm39)	missense	possibly damaging	0.73
R6460:Zfp735	UTSW	11	73,602,478 (GRCm39)	missense	probably benign	0.33
R6820:Zfp735	UTSW	11	73,579,783 (GRCm39)	start codon destroyed	probably null	0.01
R6831:Zfp735	UTSW	11	73,601,434 (GRCm39)	missense	probably damaging	1.00
R6833:Zfp735	UTSW	11	73,601,434 (GRCm39)	missense	probably damaging	1.00
R6834:Zfp735	UTSW	11	73,601,434 (GRCm39)	missense	probably damaging	1.00
R6897:Zfp735	UTSW	11	73,601,880 (GRCm39)	missense	probably benign	0.08
R6941:Zfp735	UTSW	11	73,581,159 (GRCm39)	missense	probably benign	0.33
R7335:Zfp735	UTSW	11	73,602,379 (GRCm39)	missense	possibly damaging	0.47
R7366:Zfp735	UTSW	11	73,602,979 (GRCm39)	missense	possibly damaging	0.93
R7474:Zfp735	UTSW	11	73,602,002 (GRCm39)	missense	possibly damaging	0.72
R7487:Zfp735	UTSW	11	73,581,154 (GRCm39)	missense	possibly damaging	0.53
R7583:Zfp735	UTSW	11	73,601,933 (GRCm39)	missense	possibly damaging	0.86
R7866:Zfp735	UTSW	11	73,601,629 (GRCm39)	missense	probably benign	0.00
R8005:Zfp735	UTSW	11	73,603,140 (GRCm39)	nonsense	probably null
R8500:Zfp735	UTSW	11	73,601,811 (GRCm39)	missense	possibly damaging	0.53
R8551:Zfp735	UTSW	11	73,603,122 (GRCm39)	missense	probably benign	0.06
R8754:Zfp735	UTSW	11	73,603,000 (GRCm39)	missense	possibly damaging	0.85
R8769:Zfp735	UTSW	11	73,581,127 (GRCm39)	missense	possibly damaging	0.53
R8794:Zfp735	UTSW	11	73,603,029 (GRCm39)	missense	possibly damaging	0.66
R8835:Zfp735	UTSW	11	73,601,692 (GRCm39)	missense	possibly damaging	0.53
R8869:Zfp735	UTSW	11	73,602,510 (GRCm39)	missense	possibly damaging	0.53
R8969:Zfp735	UTSW	11	73,602,699 (GRCm39)	missense	possibly damaging	0.83
R9072:Zfp735	UTSW	11	73,603,060 (GRCm39)	missense	probably benign	0.21
R9073:Zfp735	UTSW	11	73,603,060 (GRCm39)	missense	probably benign	0.21
R9193:Zfp735	UTSW	11	73,580,600 (GRCm39)	missense	possibly damaging	0.71
R9355:Zfp735	UTSW	11	73,602,362 (GRCm39)	missense	probably benign	0.01
R9414:Zfp735	UTSW	11	73,602,023 (GRCm39)	nonsense	probably null
R9456:Zfp735	UTSW	11	73,602,403 (GRCm39)	missense	possibly damaging	0.53
R9573:Zfp735	UTSW	11	73,602,936 (GRCm39)	missense	possibly damaging	0.67
R9647:Zfp735	UTSW	11	73,580,600 (GRCm39)	missense	probably damaging	0.98
R9710:Zfp735	UTSW	11	73,601,806 (GRCm39)	missense	possibly damaging	0.86
Z1176:Zfp735	UTSW	11	73,601,641 (GRCm39)	missense	probably benign	0.02

Predicted Primers

PCR Primer
(F):5'- GCTCAGTACTCAGCTCTTCAAAACCC -3'
(R):5'- ctgtggtgaGCATGAAGTTTTCAGAATC -3'

Sequencing Primer
(F):5'- tcaccaaagaatccatacaggag -3'
(R):5'- agcttttcccacattcattacac -3'

Posted On

2013-11-08