Mutagenetix > Incidental Mutation

Incidental Mutation 'R0890:Scn9a'

83452

Institutional Source

Beutler Lab

Gene Symbol

Scn9a

Ensembl Gene

ENSMUSG00000075316

Gene Name

sodium channel, voltage-gated, type IX, alpha

Synonyms

PN1

MMRRC Submission

039053-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R0890 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

66310424-66465306 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 66314079 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Alanine at position 1869 (T1869A)

Ref Sequence

ENSEMBL: ENSMUSP00000131711 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000100063] [ENSMUST00000100064] [ENSMUST00000112354] [ENSMUST00000164384] [ENSMUST00000169900]

AlphaFold

Q62205

Predicted Effect

probably damaging
Transcript: ENSMUST00000100063
AA Change: T1871A

PolyPhen 2 Score 0.996 (Sensitivity: 0.55; Specificity: 0.98)

SMART Domains

Protein: ENSMUSP00000097641
Gene: ENSMUSG00000075316
AA Change: T1871A

Domain	Start	End	E-Value	Type
low complexity region	29	49	N/A	INTRINSIC
Pfam:Ion_trans	154	403	9.5e-78	PFAM
coiled coil region	404	442	N/A	INTRINSIC
Pfam:DUF3451	465	685	1.3e-62	PFAM
Pfam:Ion_trans	768	957	9.9e-48	PFAM
Pfam:Na_trans_assoc	972	1191	2.9e-72	PFAM
low complexity region	1203	1214	N/A	INTRINSIC
Pfam:Ion_trans	1217	1445	2.8e-55	PFAM
PDB:1BYY\|A	1447	1499	9e-27	PDB
Pfam:Ion_trans	1538	1748	3.4e-52	PFAM
Pfam:PKD_channel	1599	1755	1.1e-7	PFAM
IQ	1877	1899	1.03e-3	SMART
low complexity region	1956	1972	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000100064
AA Change: T1880A

PolyPhen 2 Score 0.983 (Sensitivity: 0.75; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000097642
Gene: ENSMUSG00000075316
AA Change: T1880A

Domain	Start	End	E-Value	Type
low complexity region	29	49	N/A	INTRINSIC
Pfam:Ion_trans	125	412	2.2e-84	PFAM
low complexity region	433	446	N/A	INTRINSIC
Pfam:Na_trans_cytopl	483	693	7.5e-76	PFAM
Pfam:Ion_trans	742	977	4.1e-57	PFAM
Pfam:Na_trans_assoc	981	1185	1.4e-58	PFAM
Pfam:Ion_trans	1189	1466	7e-67	PFAM
Pfam:Ion_trans	1512	1769	1e-55	PFAM
Pfam:PKD_channel	1605	1763	2.6e-7	PFAM
IQ	1886	1908	1.03e-3	SMART
low complexity region	1965	1981	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000112354
AA Change: T1869A

PolyPhen 2 Score 0.996 (Sensitivity: 0.55; Specificity: 0.98)

SMART Domains

Protein: ENSMUSP00000107973
Gene: ENSMUSG00000075316
AA Change: T1869A

Domain	Start	End	E-Value	Type
low complexity region	29	49	N/A	INTRINSIC
Pfam:Ion_trans	154	401	1.2e-77	PFAM
coiled coil region	402	449	N/A	INTRINSIC
Pfam:DUF3451	463	683	1.3e-62	PFAM
Pfam:Ion_trans	766	955	9.9e-48	PFAM
Pfam:Na_trans_assoc	970	1189	2.9e-72	PFAM
low complexity region	1201	1212	N/A	INTRINSIC
Pfam:Ion_trans	1215	1443	2.8e-55	PFAM
PDB:1BYY\|A	1445	1497	7e-29	PDB
Pfam:Ion_trans	1536	1746	3.4e-52	PFAM
Pfam:PKD_channel	1597	1753	1.1e-7	PFAM
IQ	1875	1897	1.03e-3	SMART
low complexity region	1954	1970	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000141149

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000152740

Predicted Effect

probably damaging
Transcript: ENSMUST00000164384
AA Change: T1880A

PolyPhen 2 Score 0.983 (Sensitivity: 0.75; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000126528
Gene: ENSMUSG00000075316
AA Change: T1880A

Domain	Start	End	E-Value	Type
low complexity region	29	49	N/A	INTRINSIC
Pfam:Ion_trans	154	401	1.1e-77	PFAM
coiled coil region	402	449	N/A	INTRINSIC
Pfam:DUF3451	463	694	4.2e-66	PFAM
Pfam:Ion_trans	777	966	8.8e-48	PFAM
Pfam:Na_trans_assoc	981	1200	6e-72	PFAM
low complexity region	1212	1223	N/A	INTRINSIC
Pfam:Ion_trans	1226	1454	2.5e-55	PFAM
PDB:1BYY\|A	1456	1508	6e-29	PDB
Pfam:Ion_trans	1547	1757	3e-52	PFAM
Pfam:PKD_channel	1608	1764	8.1e-8	PFAM
IQ	1886	1908	1.03e-3	SMART
low complexity region	1965	1981	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000169900
AA Change: T1869A

PolyPhen 2 Score 0.996 (Sensitivity: 0.55; Specificity: 0.98)

SMART Domains

Protein: ENSMUSP00000131711
Gene: ENSMUSG00000075316
AA Change: T1869A

Domain	Start	End	E-Value	Type
low complexity region	29	49	N/A	INTRINSIC
Pfam:Ion_trans	154	401	3.7e-78	PFAM
coiled coil region	402	449	N/A	INTRINSIC
Pfam:DUF3451	463	683	1.3e-62	PFAM
Pfam:Ion_trans	766	955	9.9e-48	PFAM
Pfam:Na_trans_assoc	970	1189	2.9e-72	PFAM
low complexity region	1201	1212	N/A	INTRINSIC
Pfam:Ion_trans	1215	1443	2.8e-55	PFAM
PDB:1BYY\|A	1445	1497	7e-29	PDB
Pfam:Ion_trans	1536	1746	3.4e-52	PFAM
Pfam:PKD_channel	1597	1753	1.1e-7	PFAM
IQ	1875	1897	1.03e-3	SMART
low complexity region	1954	1970	N/A	INTRINSIC

Coding Region Coverage

1x: 99.4%
3x: 98.7%
10x: 97.1%
20x: 94.0%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a voltage-gated sodium channel which plays a significant role in nociception signaling. Mutations in this gene have been associated with primary erythermalgia, channelopathy-associated insensitivity to pain, and paroxysmal extreme pain disorder. [provided by RefSeq, Aug 2009]
PHENOTYPE: Mice homozygous for a knock-out allele exhibit prenatal/neonatal lethality. Mice homozygous for a knock-in allele exhibit increased susceptibility to electrically induced seizures. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 32 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca15	T	C	7: 119,972,936 (GRCm39)	S837P	probably benign	Het
Cdh24	A	G	14: 54,870,051 (GRCm39)	V240A	probably benign	Het
Clcn4	T	C	7: 7,291,964 (GRCm39)	T556A	possibly damaging	Het
Coa7	G	A	4: 108,195,583 (GRCm39)	A171T	probably damaging	Het
Col12a1	A	T	9: 79,607,684 (GRCm39)	S381R	probably damaging	Het
Col9a3	C	T	2: 180,251,856 (GRCm39)	P335L	probably benign	Het
Dcxr	T	A	11: 120,617,297 (GRCm39)	N82I	probably damaging	Het
Dhdh	T	C	7: 45,131,395 (GRCm39)	D146G	possibly damaging	Het
Dhrs13	T	A	11: 77,925,176 (GRCm39)	L99Q	probably null	Het
Dnai1	C	T	4: 41,604,253 (GRCm39)	T220M	possibly damaging	Het
Gapvd1	T	A	2: 34,602,329 (GRCm39)	D606V	probably damaging	Het
Gcn1	T	G	5: 115,717,852 (GRCm39)	C246G	possibly damaging	Het
Gdf10	A	G	14: 33,654,113 (GRCm39)	K207E	possibly damaging	Het
Gucy2d	T	C	7: 98,122,472 (GRCm39)	V1046A	probably benign	Het
Itpr3	C	A	17: 27,307,985 (GRCm39)	Y257*	probably null	Het
Kifc5b	C	T	17: 27,141,996 (GRCm39)	T158M	possibly damaging	Het
Klra7	C	T	6: 130,195,916 (GRCm39)	D251N	probably benign	Het
Mesp1	T	C	7: 79,442,683 (GRCm39)	D198G	probably benign	Het
Mrgprb8	T	A	7: 48,038,777 (GRCm39)	C149*	probably null	Het
Nphp4	C	A	4: 152,582,677 (GRCm39)	L169I	possibly damaging	Het
Or1j1	T	A	2: 36,702,586 (GRCm39)	T173S	probably benign	Het
Or52p2	C	A	7: 102,237,408 (GRCm39)	E181*	probably null	Het
Or5h17	T	A	16: 58,820,150 (GRCm39)	I34K	possibly damaging	Het
Pcgf5	A	T	19: 36,389,544 (GRCm39)	H7L	probably benign	Het
Polr3b	A	C	10: 84,550,200 (GRCm39)	K970T	probably benign	Het
Pomgnt1	A	G	4: 116,009,382 (GRCm39)	D93G	probably benign	Het
Rnf213	A	G	11: 119,321,312 (GRCm39)	K1256E	possibly damaging	Het
Setdb2	A	G	14: 59,656,669 (GRCm39)	V232A	possibly damaging	Het
Sh3d21	T	C	4: 126,044,945 (GRCm39)	E578G	probably damaging	Het
Tmem168	A	T	6: 13,603,271 (GRCm39)	S32T	probably damaging	Het
Vmn1r38	T	G	6: 66,753,514 (GRCm39)	I201L	probably benign	Het
Wfs1	C	A	5: 37,132,888 (GRCm39)	W130C	probably damaging	Het

Other mutations in Scn9a

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00514:Scn9a	APN	2	66,393,945 (GRCm39)	missense	probably damaging	1.00
IGL00570:Scn9a	APN	2	66,314,486 (GRCm39)	missense	probably damaging	1.00
IGL00809:Scn9a	APN	2	66,314,279 (GRCm39)	missense	probably damaging	1.00
IGL00977:Scn9a	APN	2	66,314,645 (GRCm39)	missense	probably damaging	0.99
IGL01120:Scn9a	APN	2	66,357,316 (GRCm39)	missense	probably benign	0.00
IGL01134:Scn9a	APN	2	66,335,312 (GRCm39)	missense	probably damaging	1.00
IGL01300:Scn9a	APN	2	66,318,397 (GRCm39)	nonsense	probably null
IGL01452:Scn9a	APN	2	66,357,416 (GRCm39)	missense	probably damaging	1.00
IGL01531:Scn9a	APN	2	66,367,722 (GRCm39)	missense	probably benign	0.11
IGL01572:Scn9a	APN	2	66,324,230 (GRCm39)	missense	probably benign	0.00
IGL01645:Scn9a	APN	2	66,317,986 (GRCm39)	missense	possibly damaging	0.62
IGL01823:Scn9a	APN	2	66,314,386 (GRCm39)	missense	probably damaging	1.00
IGL01965:Scn9a	APN	2	66,314,777 (GRCm39)	missense	probably damaging	1.00
IGL02127:Scn9a	APN	2	66,325,170 (GRCm39)	missense	probably damaging	1.00
IGL02127:Scn9a	APN	2	66,377,479 (GRCm39)	missense	probably damaging	1.00
IGL02166:Scn9a	APN	2	66,323,447 (GRCm39)	missense	possibly damaging	0.95
IGL02183:Scn9a	APN	2	66,314,955 (GRCm39)	splice site	probably benign
IGL02640:Scn9a	APN	2	66,366,440 (GRCm39)	critical splice donor site	probably null
IGL02685:Scn9a	APN	2	66,367,637 (GRCm39)	missense	probably damaging	1.00
IGL02798:Scn9a	APN	2	66,370,903 (GRCm39)	missense	possibly damaging	0.52
IGL02832:Scn9a	APN	2	66,398,373 (GRCm39)	missense	probably damaging	1.00
IGL03008:Scn9a	APN	2	66,392,855 (GRCm39)	missense	probably damaging	1.00
IGL03270:Scn9a	APN	2	66,314,358 (GRCm39)	missense	probably damaging	1.00
IGL03408:Scn9a	APN	2	66,357,091 (GRCm39)	missense	probably benign	0.00
BB007:Scn9a	UTSW	2	66,335,193 (GRCm39)	missense	probably damaging	0.99
BB017:Scn9a	UTSW	2	66,335,193 (GRCm39)	missense	probably damaging	0.99
R0039:Scn9a	UTSW	2	66,392,788 (GRCm39)	missense	probably damaging	0.98
R0173:Scn9a	UTSW	2	66,363,437 (GRCm39)	missense	probably damaging	1.00
R0323:Scn9a	UTSW	2	66,398,475 (GRCm39)	missense	probably damaging	1.00
R0344:Scn9a	UTSW	2	66,335,354 (GRCm39)	missense	probably damaging	0.99
R0421:Scn9a	UTSW	2	66,373,621 (GRCm39)	missense	probably benign
R0465:Scn9a	UTSW	2	66,357,340 (GRCm39)	missense	probably damaging	1.00
R0514:Scn9a	UTSW	2	66,314,022 (GRCm39)	missense	probably damaging	1.00
R0599:Scn9a	UTSW	2	66,357,143 (GRCm39)	missense	probably damaging	0.96
R0627:Scn9a	UTSW	2	66,367,721 (GRCm39)	missense	probably benign	0.00
R0644:Scn9a	UTSW	2	66,363,405 (GRCm39)	critical splice donor site	probably null
R0653:Scn9a	UTSW	2	66,363,721 (GRCm39)	missense	probably damaging	1.00
R0685:Scn9a	UTSW	2	66,313,843 (GRCm39)	missense	probably benign	0.02
R0718:Scn9a	UTSW	2	66,377,456 (GRCm39)	missense	probably damaging	1.00
R0827:Scn9a	UTSW	2	66,366,468 (GRCm39)	nonsense	probably null
R1139:Scn9a	UTSW	2	66,335,341 (GRCm39)	missense	probably benign	0.02
R1385:Scn9a	UTSW	2	66,393,886 (GRCm39)	missense	probably damaging	1.00
R1398:Scn9a	UTSW	2	66,314,930 (GRCm39)	missense	probably benign	0.11
R1496:Scn9a	UTSW	2	66,357,232 (GRCm39)	missense	probably benign
R1511:Scn9a	UTSW	2	66,357,157 (GRCm39)	missense	probably benign	0.01
R1517:Scn9a	UTSW	2	66,335,371 (GRCm39)	splice site	probably benign
R1564:Scn9a	UTSW	2	66,314,648 (GRCm39)	missense	probably damaging	1.00
R1634:Scn9a	UTSW	2	66,318,361 (GRCm39)	missense	probably damaging	1.00
R1662:Scn9a	UTSW	2	66,313,803 (GRCm39)	missense	probably benign	0.00
R1695:Scn9a	UTSW	2	66,335,220 (GRCm39)	nonsense	probably null
R1709:Scn9a	UTSW	2	66,313,850 (GRCm39)	missense	probably damaging	1.00
R1741:Scn9a	UTSW	2	66,317,938 (GRCm39)	missense	probably damaging	0.99
R1755:Scn9a	UTSW	2	66,332,060 (GRCm39)	missense	probably benign	0.38
R1914:Scn9a	UTSW	2	66,396,594 (GRCm39)	missense	probably damaging	1.00
R1962:Scn9a	UTSW	2	66,314,655 (GRCm39)	missense	probably damaging	1.00
R1970:Scn9a	UTSW	2	66,345,724 (GRCm39)	missense	probably damaging	0.97
R2017:Scn9a	UTSW	2	66,345,665 (GRCm39)	missense	probably damaging	0.99
R2092:Scn9a	UTSW	2	66,363,720 (GRCm39)	missense	probably damaging	0.99
R2105:Scn9a	UTSW	2	66,398,527 (GRCm39)	missense	probably benign	0.25
R2114:Scn9a	UTSW	2	66,314,396 (GRCm39)	missense	probably damaging	1.00
R2115:Scn9a	UTSW	2	66,314,396 (GRCm39)	missense	probably damaging	1.00
R2128:Scn9a	UTSW	2	66,356,998 (GRCm39)	missense	probably damaging	1.00
R2157:Scn9a	UTSW	2	66,366,669 (GRCm39)	missense	probably damaging	1.00
R2162:Scn9a	UTSW	2	66,364,573 (GRCm39)	missense	probably damaging	0.98
R2350:Scn9a	UTSW	2	66,335,312 (GRCm39)	missense	probably damaging	1.00
R3694:Scn9a	UTSW	2	66,392,749 (GRCm39)	missense	probably benign
R3771:Scn9a	UTSW	2	66,313,992 (GRCm39)	missense	probably benign	0.26
R3772:Scn9a	UTSW	2	66,313,992 (GRCm39)	missense	probably benign	0.26
R3773:Scn9a	UTSW	2	66,313,992 (GRCm39)	missense	probably benign	0.26
R3922:Scn9a	UTSW	2	66,357,217 (GRCm39)	missense	possibly damaging	0.88
R3926:Scn9a	UTSW	2	66,357,217 (GRCm39)	missense	possibly damaging	0.88
R4258:Scn9a	UTSW	2	66,395,398 (GRCm39)	intron	probably benign
R4385:Scn9a	UTSW	2	66,314,900 (GRCm39)	missense	probably damaging	1.00
R4415:Scn9a	UTSW	2	66,357,037 (GRCm39)	missense	probably damaging	1.00
R4570:Scn9a	UTSW	2	66,313,902 (GRCm39)	missense	possibly damaging	0.85
R4682:Scn9a	UTSW	2	66,377,362 (GRCm39)	missense	probably benign
R4783:Scn9a	UTSW	2	66,370,967 (GRCm39)	missense	probably benign	0.01
R4822:Scn9a	UTSW	2	66,314,093 (GRCm39)	missense	possibly damaging	0.55
R4829:Scn9a	UTSW	2	66,382,057 (GRCm39)	missense	probably benign
R4908:Scn9a	UTSW	2	66,357,087 (GRCm39)	missense	probably benign	0.03
R4983:Scn9a	UTSW	2	66,396,614 (GRCm39)	missense	probably benign	0.02
R5047:Scn9a	UTSW	2	66,392,824 (GRCm39)	missense	probably damaging	1.00
R5100:Scn9a	UTSW	2	66,364,463 (GRCm39)	missense	probably damaging	1.00
R5140:Scn9a	UTSW	2	66,395,511 (GRCm39)	missense	possibly damaging	0.81
R5398:Scn9a	UTSW	2	66,318,387 (GRCm39)	missense	probably damaging	1.00
R5557:Scn9a	UTSW	2	66,377,447 (GRCm39)	missense	probably damaging	0.99
R5582:Scn9a	UTSW	2	66,395,373 (GRCm39)	intron	probably benign
R6108:Scn9a	UTSW	2	66,314,393 (GRCm39)	missense	probably damaging	1.00
R6115:Scn9a	UTSW	2	66,393,973 (GRCm39)	missense	possibly damaging	0.70
R6143:Scn9a	UTSW	2	66,317,868 (GRCm39)	missense	probably benign	0.00
R6261:Scn9a	UTSW	2	66,314,240 (GRCm39)	missense	probably damaging	1.00
R6335:Scn9a	UTSW	2	66,398,608 (GRCm39)	start codon destroyed	possibly damaging	0.91
R6429:Scn9a	UTSW	2	66,357,307 (GRCm39)	missense	possibly damaging	0.95
R6632:Scn9a	UTSW	2	66,313,846 (GRCm39)	missense	probably benign	0.23
R6681:Scn9a	UTSW	2	66,393,686 (GRCm39)	missense	possibly damaging	0.90
R6830:Scn9a	UTSW	2	66,398,373 (GRCm39)	missense	probably damaging	1.00
R7102:Scn9a	UTSW	2	66,379,359 (GRCm39)	missense	probably damaging	1.00
R7186:Scn9a	UTSW	2	66,364,567 (GRCm39)	missense	probably damaging	1.00
R7243:Scn9a	UTSW	2	66,370,874 (GRCm39)	missense	probably damaging	1.00
R7311:Scn9a	UTSW	2	66,314,748 (GRCm39)	missense	possibly damaging	0.54
R7328:Scn9a	UTSW	2	66,314,931 (GRCm39)	missense	probably benign
R7386:Scn9a	UTSW	2	66,370,894 (GRCm39)	missense	probably damaging	1.00
R7438:Scn9a	UTSW	2	66,377,531 (GRCm39)	missense	possibly damaging	0.81
R7483:Scn9a	UTSW	2	66,363,692 (GRCm39)	missense	probably damaging	0.99
R7485:Scn9a	UTSW	2	66,364,561 (GRCm39)	missense	probably damaging	1.00
R7526:Scn9a	UTSW	2	66,313,990 (GRCm39)	missense	probably benign
R7617:Scn9a	UTSW	2	66,370,893 (GRCm39)	missense	possibly damaging	0.55
R7642:Scn9a	UTSW	2	66,366,580 (GRCm39)	missense	probably benign	0.02
R7653:Scn9a	UTSW	2	66,357,424 (GRCm39)	missense	probably damaging	1.00
R7747:Scn9a	UTSW	2	66,314,642 (GRCm39)	missense	probably damaging	1.00
R7823:Scn9a	UTSW	2	66,314,135 (GRCm39)	missense	probably damaging	1.00
R7864:Scn9a	UTSW	2	66,314,904 (GRCm39)	missense	possibly damaging	0.73
R7890:Scn9a	UTSW	2	66,373,456 (GRCm39)	missense	probably benign	0.00
R7930:Scn9a	UTSW	2	66,335,193 (GRCm39)	missense	probably damaging	0.99
R7975:Scn9a	UTSW	2	66,314,597 (GRCm39)	missense	probably damaging	1.00
R8057:Scn9a	UTSW	2	66,345,774 (GRCm39)	missense	probably benign	0.06
R8145:Scn9a	UTSW	2	66,317,754 (GRCm39)	missense	probably damaging	1.00
R8163:Scn9a	UTSW	2	66,314,745 (GRCm39)	missense	probably damaging	1.00
R8165:Scn9a	UTSW	2	66,370,874 (GRCm39)	missense	probably damaging	1.00
R8342:Scn9a	UTSW	2	66,366,626 (GRCm39)	missense	probably benign
R8345:Scn9a	UTSW	2	66,324,966 (GRCm39)	missense	probably damaging	0.96
R8464:Scn9a	UTSW	2	66,396,625 (GRCm39)	missense	probably damaging	0.99
R8467:Scn9a	UTSW	2	66,332,015 (GRCm39)	missense	probably damaging	1.00
R8698:Scn9a	UTSW	2	66,366,628 (GRCm39)	missense	probably benign	0.00
R8810:Scn9a	UTSW	2	66,332,010 (GRCm39)	missense	probably damaging	1.00
R8822:Scn9a	UTSW	2	66,370,979 (GRCm39)	missense	probably damaging	0.99
R8829:Scn9a	UTSW	2	66,313,961 (GRCm39)	missense	probably benign
R9009:Scn9a	UTSW	2	66,338,927 (GRCm39)	missense	probably damaging	1.00
R9038:Scn9a	UTSW	2	66,325,147 (GRCm39)	missense	probably damaging	1.00
R9126:Scn9a	UTSW	2	66,314,744 (GRCm39)	missense	probably damaging	1.00
R9205:Scn9a	UTSW	2	66,363,657 (GRCm39)	missense	probably damaging	1.00
R9300:Scn9a	UTSW	2	66,335,236 (GRCm39)	missense	probably benign	0.39
R9373:Scn9a	UTSW	2	66,314,261 (GRCm39)	missense	probably benign	0.00
R9404:Scn9a	UTSW	2	66,357,040 (GRCm39)	missense	probably benign	0.02
R9443:Scn9a	UTSW	2	66,395,553 (GRCm39)	missense	probably damaging	1.00
R9590:Scn9a	UTSW	2	66,314,328 (GRCm39)	missense	probably benign	0.05
R9612:Scn9a	UTSW	2	66,363,708 (GRCm39)	missense	probably damaging	1.00
R9617:Scn9a	UTSW	2	66,392,809 (GRCm39)	missense	probably damaging	1.00
R9717:Scn9a	UTSW	2	66,357,002 (GRCm39)	missense	probably benign
X0003:Scn9a	UTSW	2	66,338,991 (GRCm39)	missense	probably benign	0.02
X0062:Scn9a	UTSW	2	66,398,421 (GRCm39)	missense	probably damaging	1.00
Z1176:Scn9a	UTSW	2	66,370,936 (GRCm39)	missense	probably benign	0.00
Z1177:Scn9a	UTSW	2	66,325,029 (GRCm39)	missense	possibly damaging	0.68

Predicted Primers

PCR Primer
(F):5'- AGTCACCTTCGCAGGCTGTAAAC -3'
(R):5'- ACGAAGTCTGGGAGAAGTTCGACC -3'

Sequencing Primer
(F):5'- CGCAGGCTGTAAACAGTGTATC -3'
(R):5'- GAGTTCTGCAAGCTCTCTGAC -3'

Posted On

2013-11-08