Mutagenetix > Incidental Mutation

Incidental Mutation 'R0894:Kdm2b'

83651

Institutional Source

Beutler Lab

Gene Symbol

Kdm2b

Ensembl Gene

ENSMUSG00000029475

Gene Name

lysine (K)-specific demethylase 2B

Synonyms

Cxxc2, Fbxl10, Jhdm1b

MMRRC Submission

039057-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R0894 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

123008727-123127333 bp(-) (GRCm39)

Type of Mutation

critical splice donor site (2 bp from exon)

DNA Base Change (assembly)

A to G at 123122523 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000118488 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000046073] [ENSMUST00000086200] [ENSMUST00000086200] [ENSMUST00000118027] [ENSMUST00000118027] [ENSMUST00000121739] [ENSMUST00000121739] [ENSMUST00000127403] [ENSMUST00000156474] [ENSMUST00000156474]

AlphaFold

Q6P1G2

Predicted Effect

probably null
Transcript: ENSMUST00000046073

SMART Domains

Protein: ENSMUSP00000038229
Gene: ENSMUSG00000029475

Domain	Start	End	E-Value	Type
Blast:JmjC	23	101	4e-41	BLAST
JmjC	147	315	3.61e-41	SMART
low complexity region	380	401	N/A	INTRINSIC
low complexity region	406	424	N/A	INTRINSIC
PDB:2YU2\|A	472	546	1e-17	PDB
Pfam:zf-CXXC	578	624	3e-17	PFAM
PHD	634	696	8.58e-4	SMART
low complexity region	792	823	N/A	INTRINSIC
low complexity region	903	913	N/A	INTRINSIC
low complexity region	990	1006	N/A	INTRINSIC
low complexity region	1018	1032	N/A	INTRINSIC
FBOX	1038	1078	1.69e-2	SMART
LRR	1121	1143	1.31e2	SMART
LRR	1145	1170	2.9e2	SMART
LRR	1185	1209	2.04e2	SMART
LRR	1210	1235	1.1e1	SMART
LRR	1265	1290	3.91e1	SMART

Predicted Effect

probably null
Transcript: ENSMUST00000086200

SMART Domains

Protein: ENSMUSP00000083376
Gene: ENSMUSG00000029475

Domain	Start	End	E-Value	Type
Blast:JmjC	17	95	4e-41	BLAST
JmjC	141	309	3.61e-41	SMART
low complexity region	374	395	N/A	INTRINSIC
low complexity region	400	418	N/A	INTRINSIC
PDB:2YU2\|A	466	540	1e-17	PDB
Pfam:zf-CXXC	572	618	2.1e-17	PFAM
PHD	628	690	8.58e-4	SMART
low complexity region	786	817	N/A	INTRINSIC
low complexity region	897	907	N/A	INTRINSIC
low complexity region	984	1000	N/A	INTRINSIC
low complexity region	1012	1026	N/A	INTRINSIC
FBOX	1032	1072	1.69e-2	SMART
LRR	1115	1137	1.31e2	SMART
LRR	1139	1164	2.9e2	SMART
LRR	1179	1203	2.04e2	SMART
LRR	1204	1229	1.1e1	SMART
LRR	1259	1284	3.91e1	SMART

Predicted Effect

probably null
Transcript: ENSMUST00000086200

SMART Domains

Protein: ENSMUSP00000083376
Gene: ENSMUSG00000029475

Domain	Start	End	E-Value	Type
Blast:JmjC	17	95	4e-41	BLAST
JmjC	141	309	3.61e-41	SMART
low complexity region	374	395	N/A	INTRINSIC
low complexity region	400	418	N/A	INTRINSIC
PDB:2YU2\|A	466	540	1e-17	PDB
Pfam:zf-CXXC	572	618	2.1e-17	PFAM
PHD	628	690	8.58e-4	SMART
low complexity region	786	817	N/A	INTRINSIC
low complexity region	897	907	N/A	INTRINSIC
low complexity region	984	1000	N/A	INTRINSIC
low complexity region	1012	1026	N/A	INTRINSIC
FBOX	1032	1072	1.69e-2	SMART
LRR	1115	1137	1.31e2	SMART
LRR	1139	1164	2.9e2	SMART
LRR	1179	1203	2.04e2	SMART
LRR	1204	1229	1.1e1	SMART
LRR	1259	1284	3.91e1	SMART

Predicted Effect

probably null
Transcript: ENSMUST00000118027

SMART Domains

Protein: ENSMUSP00000114052
Gene: ENSMUSG00000029475

Domain	Start	End	E-Value	Type
Blast:JmjC	23	101	4e-41	BLAST
JmjC	147	315	3.61e-41	SMART
low complexity region	380	401	N/A	INTRINSIC
low complexity region	406	424	N/A	INTRINSIC
PDB:2YU2\|A	472	546	9e-18	PDB
Pfam:zf-CXXC	578	624	2.1e-17	PFAM
PHD	634	696	8.58e-4	SMART
low complexity region	865	875	N/A	INTRINSIC
low complexity region	952	968	N/A	INTRINSIC
low complexity region	980	994	N/A	INTRINSIC
FBOX	1000	1040	1.69e-2	SMART
LRR	1083	1105	1.31e2	SMART
LRR	1107	1132	2.9e2	SMART
LRR	1147	1171	2.04e2	SMART
LRR	1172	1197	1.1e1	SMART
LRR	1227	1252	3.91e1	SMART

Predicted Effect

probably null
Transcript: ENSMUST00000118027

SMART Domains

Protein: ENSMUSP00000114052
Gene: ENSMUSG00000029475

Domain	Start	End	E-Value	Type
Blast:JmjC	23	101	4e-41	BLAST
JmjC	147	315	3.61e-41	SMART
low complexity region	380	401	N/A	INTRINSIC
low complexity region	406	424	N/A	INTRINSIC
PDB:2YU2\|A	472	546	9e-18	PDB
Pfam:zf-CXXC	578	624	2.1e-17	PFAM
PHD	634	696	8.58e-4	SMART
low complexity region	865	875	N/A	INTRINSIC
low complexity region	952	968	N/A	INTRINSIC
low complexity region	980	994	N/A	INTRINSIC
FBOX	1000	1040	1.69e-2	SMART
LRR	1083	1105	1.31e2	SMART
LRR	1107	1132	2.9e2	SMART
LRR	1147	1171	2.04e2	SMART
LRR	1172	1197	1.1e1	SMART
LRR	1227	1252	3.91e1	SMART

Predicted Effect

probably null
Transcript: ENSMUST00000121739

SMART Domains

Protein: ENSMUSP00000114049
Gene: ENSMUSG00000029475

Domain	Start	End	E-Value	Type
Blast:JmjC	1	46	2e-19	BLAST
JmjC	92	260	3.61e-41	SMART
low complexity region	325	346	N/A	INTRINSIC
low complexity region	351	369	N/A	INTRINSIC
PDB:2YU2\|A	417	491	1e-17	PDB
Pfam:zf-CXXC	523	569	5.4e-17	PFAM
PHD	579	641	8.58e-4	SMART
low complexity region	737	768	N/A	INTRINSIC
low complexity region	848	858	N/A	INTRINSIC
low complexity region	935	951	N/A	INTRINSIC
low complexity region	963	977	N/A	INTRINSIC
FBOX	983	1023	1.69e-2	SMART
LRR	1066	1088	1.31e2	SMART
LRR	1090	1115	2.9e2	SMART
LRR	1130	1154	2.04e2	SMART
LRR	1155	1180	1.1e1	SMART
LRR	1210	1235	3.91e1	SMART

Predicted Effect

probably null
Transcript: ENSMUST00000121739

SMART Domains

Protein: ENSMUSP00000114049
Gene: ENSMUSG00000029475

Domain	Start	End	E-Value	Type
Blast:JmjC	1	46	2e-19	BLAST
JmjC	92	260	3.61e-41	SMART
low complexity region	325	346	N/A	INTRINSIC
low complexity region	351	369	N/A	INTRINSIC
PDB:2YU2\|A	417	491	1e-17	PDB
Pfam:zf-CXXC	523	569	5.4e-17	PFAM
PHD	579	641	8.58e-4	SMART
low complexity region	737	768	N/A	INTRINSIC
low complexity region	848	858	N/A	INTRINSIC
low complexity region	935	951	N/A	INTRINSIC
low complexity region	963	977	N/A	INTRINSIC
FBOX	983	1023	1.69e-2	SMART
LRR	1066	1088	1.31e2	SMART
LRR	1090	1115	2.9e2	SMART
LRR	1130	1154	2.04e2	SMART
LRR	1155	1180	1.1e1	SMART
LRR	1210	1235	3.91e1	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000123479

Predicted Effect

probably benign
Transcript: ENSMUST00000127403

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000134501

Predicted Effect

probably null
Transcript: ENSMUST00000156474

SMART Domains

Protein: ENSMUSP00000118488
Gene: ENSMUSG00000029475

Domain	Start	End	E-Value	Type
Blast:JmjC	17	95	3e-42	BLAST
JmjC	141	309	3.61e-41	SMART
low complexity region	374	395	N/A	INTRINSIC
low complexity region	400	418	N/A	INTRINSIC
PDB:2YU2\|A	466	512	1e-8	PDB

Predicted Effect

probably null
Transcript: ENSMUST00000156474

SMART Domains

Protein: ENSMUSP00000118488
Gene: ENSMUSG00000029475

Domain	Start	End	E-Value	Type
Blast:JmjC	17	95	3e-42	BLAST
JmjC	141	309	3.61e-41	SMART
low complexity region	374	395	N/A	INTRINSIC
low complexity region	400	418	N/A	INTRINSIC
PDB:2YU2\|A	466	512	1e-8	PDB

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000172729

SMART Domains

Protein: ENSMUSP00000133329
Gene: ENSMUSG00000029475

Domain	Start	End	E-Value	Type
Blast:JmjC	76	105	8e-11	BLAST

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000150543

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000148466

Meta Mutation Damage Score

0.9585

Coding Region Coverage

1x: 99.5%
3x: 99.0%
10x: 97.7%
20x: 95.8%

Validation Efficiency

97% (102/105)

MGI Phenotype

FUNCTION: The protein encoded by this gene is a H3K36-specific histone demethylase, which contains an N-terminal jumonji C domain, a CxxC zinc finger domain, a plant homeodomain finger, an F-box, and eight leucine-rich repeats. Amongst its demonstrated functions, this protein plays roles in the suppression of premature cellular senescence, leukemia maintenance and development, maintenance of mouse embryonic stem cell pluripotency, and induced pluripotent stem cell generation. Mice homozygous for a targeted deletion of the zinc finger domain display embryonic lethality with development ceasing at approximately 7 to 8 days post coitum, demonstrating an essential role in early development. A pseudogene of this gene is found on chromosome 4. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014]
PHENOTYPE: Mice homozygous for a targeted allele that does not express the long form protein exhibit exencephaly, fetal and postnatal lethality, coloboma, curly tail, oligozoospermia, increased apoptosis, and increased neuronal precursor proliferation. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 104 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2210408I21Rik	A	G	13: 77,471,726 (GRCm39)	S1044G	probably benign	Het
Aanat	A	G	11: 116,487,730 (GRCm39)	H143R	probably benign	Het
Abca8a	A	G	11: 109,941,792 (GRCm39)	I1159T	probably benign	Het
Abcb1a	G	T	5: 8,724,856 (GRCm39)		probably benign	Het
Abcc1	T	C	16: 14,283,001 (GRCm39)	V1159A	possibly damaging	Het
Akr1e1	T	A	13: 4,645,071 (GRCm39)	Q204L	probably damaging	Het
Alk	T	C	17: 72,202,930 (GRCm39)	Y1135C	probably damaging	Het
Atad2b	C	T	12: 5,015,915 (GRCm39)	T547I	probably damaging	Het
Brd10	T	C	19: 29,697,974 (GRCm39)		probably benign	Het
C030005K15Rik	A	T	10: 97,561,648 (GRCm39)	S28T	unknown	Het
Cdk5rap3	A	G	11: 96,799,654 (GRCm39)	L387P	probably damaging	Het
Cfap95	T	C	19: 23,630,062 (GRCm39)	E10G	unknown	Het
Cfap96	A	G	8: 46,409,497 (GRCm39)	F274S	probably damaging	Het
Clec4f	T	A	6: 83,629,979 (GRCm39)	N193I	probably damaging	Het
Col4a4	A	T	1: 82,507,377 (GRCm39)		probably null	Het
Cplx4	T	G	18: 66,090,116 (GRCm39)	D101A	possibly damaging	Het
Cpne8	A	T	15: 90,533,474 (GRCm39)	D50E	probably damaging	Het
Csmd3	G	T	15: 47,721,316 (GRCm39)	D1542E	possibly damaging	Het
Ctdp1	A	G	18: 80,512,736 (GRCm39)	V9A	probably benign	Het
Ctnnd2	A	T	15: 30,332,301 (GRCm39)		probably benign	Het
Cyp7b1	C	T	3: 18,151,674 (GRCm39)	A180T	probably benign	Het
Dcun1d5	C	T	9: 7,203,379 (GRCm39)		probably benign	Het
Dgat1	G	T	15: 76,387,199 (GRCm39)	L363I	possibly damaging	Het
Dipk2b	T	A	X: 18,289,791 (GRCm39)	I224F	possibly damaging	Het
Dlg1	T	A	16: 31,561,965 (GRCm39)	H120Q	probably benign	Het
Dnah6	T	C	6: 73,101,740 (GRCm39)	N1928S	probably benign	Het
Dnai4	T	C	4: 102,906,583 (GRCm39)		probably benign	Het
Dync2h1	A	T	9: 7,041,734 (GRCm39)		probably benign	Het
Ednra	T	G	8: 78,446,649 (GRCm39)		probably benign	Het
Efcab6	A	T	15: 83,802,493 (GRCm39)	C845S	probably benign	Het
Egln1	A	T	8: 125,642,435 (GRCm39)	C303S	probably damaging	Het
Eomes	T	C	9: 118,311,368 (GRCm39)		probably null	Het
Epha1	A	G	6: 42,340,756 (GRCm39)	V568A	probably benign	Het
Ercc6	T	A	14: 32,238,985 (GRCm39)	N24K	probably benign	Het
Esco2	T	C	14: 66,064,726 (GRCm39)	Q338R	probably benign	Het
Fbxo46	T	C	7: 18,869,654 (GRCm39)	V91A	probably damaging	Het
Fryl	A	T	5: 73,198,675 (GRCm39)		probably benign	Het
Gab3	A	C	X: 74,077,024 (GRCm39)	D43E	probably damaging	Het
Gltpd2	T	A	11: 70,410,535 (GRCm39)		probably benign	Het
Gm17333	G	T	16: 77,649,711 (GRCm39)		noncoding transcript	Het
Gm7353	T	C	7: 3,160,570 (GRCm39)		noncoding transcript	Het
Grik4	T	C	9: 42,599,405 (GRCm39)		probably benign	Het
Gtpbp2	G	T	17: 46,476,895 (GRCm39)	A358S	possibly damaging	Het
Hyls1	C	T	9: 35,472,528 (GRCm39)	C296Y	probably damaging	Het
Igf2r	C	T	17: 12,910,988 (GRCm39)	M1943I	probably benign	Het
Iqca1l	T	C	5: 24,755,731 (GRCm39)		probably null	Het
Ireb2	T	A	9: 54,803,861 (GRCm39)	N517K	probably damaging	Het
Itga10	A	G	3: 96,560,976 (GRCm39)	S614G	possibly damaging	Het
Kif17	T	C	4: 138,025,542 (GRCm39)	M948T	possibly damaging	Het
Klhl33	A	T	14: 51,129,583 (GRCm39)	N347K	probably damaging	Het
Llph	T	A	10: 120,064,086 (GRCm39)	C67*	probably null	Het
Lrrn3	T	C	12: 41,504,033 (GRCm39)	T95A	probably damaging	Het
Map3k12	C	A	15: 102,410,613 (GRCm39)	A455S	probably damaging	Het
Mex3d	T	C	10: 80,217,376 (GRCm39)	T149A	probably benign	Het
Myo7b	A	G	18: 32,133,123 (GRCm39)	W409R	probably damaging	Het
Nbea	A	G	3: 55,916,761 (GRCm39)	M833T	possibly damaging	Het
Ncapg	A	G	5: 45,837,236 (GRCm39)	T436A	probably null	Het
Nkx1-2	C	A	7: 132,201,042 (GRCm39)	D72Y	probably null	Het
Or11g27	T	C	14: 50,771,159 (GRCm39)	S97P	possibly damaging	Het
Or2d3b	T	A	7: 106,514,317 (GRCm39)	I304K	probably benign	Het
Or6c217	T	A	10: 129,737,751 (GRCm39)	N276I	probably damaging	Het
Pcdh15	A	G	10: 74,460,087 (GRCm39)	Y1308C	probably damaging	Het
Pcnx2	A	G	8: 126,613,665 (GRCm39)		probably benign	Het
Pcsk1	G	A	13: 75,246,096 (GRCm39)	G158D	probably damaging	Het
Phkb	A	T	8: 86,744,070 (GRCm39)	D573V	probably damaging	Het
Pik3r4	A	G	9: 105,544,970 (GRCm39)	K150E	possibly damaging	Het
Ppp2r5e	C	G	12: 75,516,341 (GRCm39)	A239P	probably damaging	Het
Ppp4r4	C	T	12: 103,566,754 (GRCm39)	A67V	probably damaging	Het
Prex2	A	G	1: 11,252,122 (GRCm39)	T1056A	probably benign	Het
Prkca	A	T	11: 107,903,518 (GRCm39)	Y285N	possibly damaging	Het
Psd	T	C	19: 46,301,880 (GRCm39)	E903G	probably damaging	Het
Psg19	T	C	7: 18,527,987 (GRCm39)	E252G	probably benign	Het
Psg20	T	A	7: 18,414,969 (GRCm39)	K306*	probably null	Het
Pygl	T	G	12: 70,241,148 (GRCm39)		probably benign	Het
Rasgrf2	A	G	13: 92,130,890 (GRCm39)	S724P	probably damaging	Het
Reck	C	A	4: 43,922,967 (GRCm39)	A414D	probably damaging	Het
Scn10a	C	A	9: 119,459,213 (GRCm39)	V1150L	probably damaging	Het
Shc2	A	T	10: 79,465,751 (GRCm39)	I187N	probably damaging	Het
Sipa1l3	T	A	7: 29,086,716 (GRCm39)	K625*	probably null	Het
Slc44a4	T	C	17: 35,147,466 (GRCm39)	L583P	possibly damaging	Het
Slc5a11	G	C	7: 122,857,643 (GRCm39)	R244P	possibly damaging	Het
Slfn8	T	A	11: 82,894,407 (GRCm39)	Q744L	probably benign	Het
Snx2	G	T	18: 53,309,488 (GRCm39)	V13L	probably benign	Het
Spmip5	A	T	19: 58,776,015 (GRCm39)	L138Q	probably damaging	Het
Spsb1	C	T	4: 149,990,872 (GRCm39)		probably null	Het
Stfa2l1	A	T	16: 35,977,228 (GRCm39)	I8L	probably benign	Het
Svil	G	T	18: 5,097,494 (GRCm39)	R1659L	probably damaging	Het
Tbccd1	A	T	16: 22,640,995 (GRCm39)	L461M	probably benign	Het
Tmem9	A	T	1: 135,961,926 (GRCm39)	T174S	possibly damaging	Het
Tnks2	T	C	19: 36,867,450 (GRCm39)		probably null	Het
Tnrc18	C	A	5: 142,800,869 (GRCm39)	V30L	probably benign	Het
Tomm7	A	G	5: 24,049,025 (GRCm39)	F16S	probably damaging	Het
Ttf2	A	G	3: 100,876,865 (GRCm39)		probably benign	Het
Ubr7	C	A	12: 102,735,450 (GRCm39)	T303N	probably damaging	Het
Ushbp1	A	T	8: 71,842,868 (GRCm39)		probably null	Het
Vmn1r177	C	A	7: 23,565,475 (GRCm39)	V134F	probably benign	Het
Vmn2r12	A	G	5: 109,235,716 (GRCm39)		probably null	Het
Vmn2r53	T	G	7: 12,335,141 (GRCm39)	H173P	probably benign	Het
Yars1	A	T	4: 129,090,948 (GRCm39)	M119L	probably damaging	Het
Zcrb1	A	T	15: 93,295,038 (GRCm39)		probably benign	Het
Zfp267	T	A	3: 36,218,935 (GRCm39)	Y319*	probably null	Het
Zfp319	C	A	8: 96,056,250 (GRCm39)		probably benign	Het
Zfp783	C	G	6: 47,920,320 (GRCm39)		noncoding transcript	Het
Zfyve26	A	G	12: 79,320,372 (GRCm39)	I1024T	possibly damaging	Het

Other mutations in Kdm2b

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00578:Kdm2b	APN	5	123,099,630 (GRCm39)	missense	probably damaging	1.00
IGL02061:Kdm2b	APN	5	123,021,404 (GRCm39)	missense	probably damaging	1.00
IGL02142:Kdm2b	APN	5	123,085,898 (GRCm39)	missense	probably damaging	0.99
IGL02143:Kdm2b	APN	5	123,085,898 (GRCm39)	missense	probably damaging	0.99
IGL02147:Kdm2b	APN	5	123,085,898 (GRCm39)	missense	probably damaging	0.99
IGL02294:Kdm2b	APN	5	123,099,537 (GRCm39)	missense	probably damaging	1.00
IGL02309:Kdm2b	APN	5	123,085,883 (GRCm39)	missense	probably damaging	0.99
IGL03039:Kdm2b	APN	5	123,019,734 (GRCm39)	missense	probably benign	0.06
IGL03134:Kdm2b	UTSW	5	123,070,737 (GRCm39)	missense	probably damaging	1.00
PIT4520001:Kdm2b	UTSW	5	123,079,110 (GRCm39)	missense	probably damaging	1.00
R0008:Kdm2b	UTSW	5	123,019,806 (GRCm39)	missense	probably benign	0.08
R0592:Kdm2b	UTSW	5	123,099,197 (GRCm39)	splice site	probably benign
R1078:Kdm2b	UTSW	5	123,099,604 (GRCm39)	missense	possibly damaging	0.83
R1387:Kdm2b	UTSW	5	123,018,331 (GRCm39)	missense	probably damaging	1.00
R1441:Kdm2b	UTSW	5	123,070,943 (GRCm39)	missense	probably benign	0.25
R1550:Kdm2b	UTSW	5	123,019,120 (GRCm39)	missense	probably damaging	1.00
R1795:Kdm2b	UTSW	5	123,122,523 (GRCm39)	critical splice donor site	probably null
R2060:Kdm2b	UTSW	5	123,021,428 (GRCm39)	missense	probably damaging	1.00
R2161:Kdm2b	UTSW	5	123,018,762 (GRCm39)	missense	probably damaging	1.00
R2259:Kdm2b	UTSW	5	123,020,479 (GRCm39)	missense	probably damaging	1.00
R3843:Kdm2b	UTSW	5	123,072,856 (GRCm39)	missense	probably damaging	0.98
R3844:Kdm2b	UTSW	5	123,072,856 (GRCm39)	missense	probably damaging	0.98
R3859:Kdm2b	UTSW	5	123,018,290 (GRCm39)	missense	probably damaging	1.00
R4506:Kdm2b	UTSW	5	123,026,688 (GRCm39)	missense	possibly damaging	0.58
R4680:Kdm2b	UTSW	5	123,072,849 (GRCm39)	missense	probably damaging	0.99
R4786:Kdm2b	UTSW	5	123,018,917 (GRCm39)	critical splice acceptor site	probably null
R4894:Kdm2b	UTSW	5	123,079,030 (GRCm39)	nonsense	probably null
R5265:Kdm2b	UTSW	5	123,016,651 (GRCm39)	missense	probably damaging	1.00
R5522:Kdm2b	UTSW	5	123,087,225 (GRCm39)	missense	probably damaging	1.00
R5746:Kdm2b	UTSW	5	123,017,427 (GRCm39)	missense	probably damaging	1.00
R5813:Kdm2b	UTSW	5	123,009,931 (GRCm39)	missense	probably benign	0.37
R5920:Kdm2b	UTSW	5	123,018,359 (GRCm39)	missense	probably damaging	1.00
R5961:Kdm2b	UTSW	5	123,070,724 (GRCm39)	missense	probably benign	0.37
R6029:Kdm2b	UTSW	5	123,017,650 (GRCm39)	missense	probably damaging	1.00
R6280:Kdm2b	UTSW	5	123,016,687 (GRCm39)	missense	probably damaging	1.00
R6303:Kdm2b	UTSW	5	123,019,807 (GRCm39)	missense	probably benign	0.34
R6304:Kdm2b	UTSW	5	123,019,807 (GRCm39)	missense	probably benign	0.34
R6383:Kdm2b	UTSW	5	123,072,841 (GRCm39)	missense	probably damaging	1.00
R6432:Kdm2b	UTSW	5	123,018,254 (GRCm39)	missense	probably damaging	1.00
R6513:Kdm2b	UTSW	5	123,018,302 (GRCm39)	missense	probably damaging	0.99
R6526:Kdm2b	UTSW	5	123,099,532 (GRCm39)	missense	probably damaging	1.00
R7213:Kdm2b	UTSW	5	123,059,532 (GRCm39)	missense	probably damaging	0.99
R7226:Kdm2b	UTSW	5	123,059,512 (GRCm39)	missense	possibly damaging	0.60
R7292:Kdm2b	UTSW	5	123,018,854 (GRCm39)	missense	probably damaging	0.98
R7893:Kdm2b	UTSW	5	123,085,802 (GRCm39)	missense	probably benign	0.12
R8021:Kdm2b	UTSW	5	123,070,982 (GRCm39)	missense	probably damaging	0.99
R8038:Kdm2b	UTSW	5	123,098,958 (GRCm39)	intron	probably benign
R8162:Kdm2b	UTSW	5	123,072,856 (GRCm39)	missense	probably damaging	0.98
R8397:Kdm2b	UTSW	5	123,018,579 (GRCm39)	missense	probably benign	0.03
R8411:Kdm2b	UTSW	5	123,018,239 (GRCm39)	missense	probably damaging	1.00
R8899:Kdm2b	UTSW	5	123,125,851 (GRCm39)	nonsense	probably null
R8997:Kdm2b	UTSW	5	123,018,236 (GRCm39)	missense	probably null	0.99
R9142:Kdm2b	UTSW	5	123,127,112 (GRCm39)	unclassified	probably benign
R9192:Kdm2b	UTSW	5	123,070,679 (GRCm39)	missense	probably benign	0.05
R9238:Kdm2b	UTSW	5	123,009,889 (GRCm39)	missense	probably damaging	0.98
R9455:Kdm2b	UTSW	5	123,099,537 (GRCm39)	missense	probably damaging	1.00
R9644:Kdm2b	UTSW	5	123,120,842 (GRCm39)	missense	probably damaging	0.98
R9731:Kdm2b	UTSW	5	123,125,823 (GRCm39)	missense	probably benign	0.03
Z1177:Kdm2b	UTSW	5	123,018,860 (GRCm39)	missense	probably damaging	0.98

Predicted Primers

PCR Primer
(F):5'- GGGGAGGTCATCACTGCTTAAATGC -3'
(R):5'- CCTTGGGCTGTTCATTGACAAGTTG -3'

Sequencing Primer
(F):5'- GAAAGAGCAGCTTTTCTTCTCCAG -3'
(R):5'- CTGTTCATTGACAAGTTGGGACC -3'

Posted On

2013-11-08