Incidental Mutation 'R1073:Zfp866'
| ID |
85529 |
| Institutional Source |
Beutler Lab
|
| Gene Symbol |
Zfp866
|
| Ensembl Gene |
ENSMUSG00000043090 |
| Gene Name |
zinc finger protein 866 |
| Synonyms |
9830167H18Rik, D330038O06Rik |
| MMRRC Submission |
039159-MU
|
| Accession Numbers |
|
| Essential gene? |
Probably non essential
(E-score: 0.078)
|
| Stock # |
R1073 (G1)
|
| Quality Score |
225 |
|
Status
|
Validated
|
| Chromosome |
8 |
| Chromosomal Location |
70213974-70227561 bp(-) (GRCm39) |
| Type of Mutation |
splice site |
| DNA Base Change (assembly) |
A to T
at 70220272 bp (GRCm39)
|
| Zygosity |
Heterozygous |
| Amino Acid Change |
|
| Ref Sequence |
ENSEMBL: ENSMUSP00000123252
(fasta)
|
| Gene Model |
predicted gene model for transcript(s):
[ENSMUST00000131784]
[ENSMUST00000137573]
[ENSMUST00000149782]
|
| AlphaFold |
Q6PGD2 |
| Predicted Effect |
probably benign
Transcript: ENSMUST00000126915
|
| SMART Domains |
Protein: ENSMUSP00000122850
Gene: ENSMUSG00000092544
| Domain | Start | End | E-Value | Type |
|---|
|
KRAB
|
16 |
73 |
1.96e-17 |
SMART |
|
PDB:2I13|B
|
104 |
210 |
3e-6 |
PDB |
|
SCOP:d1fgja_
|
114 |
216 |
2e-6 |
SMART |
|
Blast:KRAB
|
189 |
209 |
3e-6 |
BLAST |
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000131784
|
| SMART Domains |
Protein: ENSMUSP00000116972
Gene: ENSMUSG00000043090
| Domain | Start | End | E-Value | Type |
|---|
|
Blast:KRAB
|
1 |
35 |
2e-19 |
BLAST |
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000137573
|
| SMART Domains |
Protein: ENSMUSP00000119781
Gene: ENSMUSG00000043090
| Domain | Start | End | E-Value | Type |
|---|
|
KRAB
|
4 |
67 |
4.26e-18 |
SMART |
|
ZnF_C2H2
|
162 |
184 |
6.42e-4 |
SMART |
|
ZnF_C2H2
|
190 |
212 |
3.69e-4 |
SMART |
|
ZnF_C2H2
|
218 |
240 |
3.89e-3 |
SMART |
|
ZnF_C2H2
|
246 |
268 |
3.34e-2 |
SMART |
|
ZnF_C2H2
|
274 |
296 |
1.76e-1 |
SMART |
|
ZnF_C2H2
|
302 |
324 |
5.42e-2 |
SMART |
|
ZnF_C2H2
|
330 |
352 |
1.38e-3 |
SMART |
|
ZnF_C2H2
|
358 |
380 |
6.78e-3 |
SMART |
|
ZnF_C2H2
|
386 |
408 |
2.09e-3 |
SMART |
|
ZnF_C2H2
|
414 |
436 |
9.44e-2 |
SMART |
|
ZnF_C2H2
|
442 |
464 |
2.95e-3 |
SMART |
|
ZnF_C2H2
|
470 |
492 |
9.73e-4 |
SMART |
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000149782
|
| SMART Domains |
Protein: ENSMUSP00000123252
Gene: ENSMUSG00000092544
| Domain | Start | End | E-Value | Type |
|---|
|
KRAB
|
80 |
139 |
1.2e-17 |
SMART |
|
ZnF_C2H2
|
155 |
177 |
1.69e-3 |
SMART |
|
ZnF_C2H2
|
182 |
204 |
8.47e-4 |
SMART |
|
ZnF_C2H2
|
210 |
232 |
7.15e-2 |
SMART |
|
| Meta Mutation Damage Score |
0.0898 |
| Coding Region Coverage |
- 1x: 99.7%
- 3x: 99.1%
- 10x: 97.5%
- 20x: 94.5%
|
| Validation Efficiency |
98% (40/41) |
| Allele List at MGI |
|
| Other mutations in this stock |
Total: 34 list
| Gene | Ref | Var | Chr/Loc | Mutation | Predicted Effect | Zygosity |
|---|
| Asap3 |
T |
C |
4: 135,963,742 (GRCm39) |
I334T |
probably damaging |
Het |
| Atxn7l3 |
C |
G |
11: 102,183,261 (GRCm39) |
|
probably benign |
Het |
| Cacna2d3 |
T |
A |
14: 28,767,580 (GRCm39) |
H765L |
probably damaging |
Het |
| Cngb1 |
A |
G |
8: 96,030,195 (GRCm39) |
|
probably null |
Het |
| Csmd1 |
C |
T |
8: 16,408,477 (GRCm39) |
|
probably benign |
Het |
| Cux1 |
A |
T |
5: 136,281,395 (GRCm39) |
|
probably null |
Het |
| D7Ertd443e |
T |
C |
7: 133,871,947 (GRCm39) |
K232R |
probably damaging |
Het |
| Dock6 |
A |
G |
9: 21,757,814 (GRCm39) |
S97P |
probably benign |
Het |
| Eml5 |
G |
A |
12: 98,797,232 (GRCm39) |
A1099V |
probably damaging |
Het |
| Gtf2h1 |
G |
A |
7: 46,466,368 (GRCm39) |
A472T |
probably damaging |
Het |
| Krt82 |
T |
A |
15: 101,458,689 (GRCm39) |
D117V |
probably damaging |
Het |
| Lrfn5 |
A |
G |
12: 61,887,595 (GRCm39) |
Y461C |
probably damaging |
Het |
| Mmrn2 |
G |
A |
14: 34,118,251 (GRCm39) |
|
probably null |
Het |
| Mrtfb |
T |
A |
16: 13,230,182 (GRCm39) |
S956T |
possibly damaging |
Het |
| Msrb3 |
T |
A |
10: 120,620,041 (GRCm39) |
S93C |
possibly damaging |
Het |
| Ncl |
AAAGCCTCCC |
AAAGCCTCCCAAGCCTCCC |
1: 86,278,538 (GRCm39) |
|
probably benign |
Het |
| Or8k41 |
A |
G |
2: 86,313,984 (GRCm39) |
I34T |
probably damaging |
Het |
| Osbpl10 |
C |
T |
9: 115,036,621 (GRCm39) |
Q381* |
probably null |
Het |
| Pelp1 |
A |
G |
11: 70,287,416 (GRCm39) |
L464P |
probably damaging |
Het |
| Pigs |
A |
G |
11: 78,226,431 (GRCm39) |
D216G |
probably benign |
Het |
| Ptprk |
T |
C |
10: 28,372,943 (GRCm39) |
|
probably null |
Het |
| Pyroxd1 |
T |
C |
6: 142,294,370 (GRCm39) |
|
probably null |
Het |
| Ros1 |
T |
A |
10: 51,922,221 (GRCm39) |
D2284V |
probably damaging |
Het |
| Rptor |
T |
C |
11: 119,634,717 (GRCm39) |
S178P |
possibly damaging |
Het |
| Slc8a1 |
C |
T |
17: 81,955,836 (GRCm39) |
D401N |
probably damaging |
Het |
| Tas2r143 |
T |
C |
6: 42,377,694 (GRCm39) |
Y175H |
probably benign |
Het |
| Tdrd9 |
T |
C |
12: 111,989,693 (GRCm39) |
S502P |
probably damaging |
Het |
| Tmem38a |
A |
G |
8: 73,333,947 (GRCm39) |
H142R |
probably damaging |
Het |
| Tmem44 |
A |
G |
16: 30,333,651 (GRCm39) |
|
probably benign |
Het |
| Ugt2b38 |
T |
G |
5: 87,560,232 (GRCm39) |
N361H |
probably damaging |
Het |
| Umod |
A |
G |
7: 119,063,964 (GRCm39) |
V614A |
possibly damaging |
Het |
| Vmn2r5 |
A |
T |
3: 64,398,726 (GRCm39) |
L751* |
probably null |
Het |
| Wdr37 |
A |
T |
13: 8,855,876 (GRCm39) |
I489N |
probably damaging |
Het |
| Xdh |
T |
C |
17: 74,246,831 (GRCm39) |
T78A |
probably benign |
Het |
|
| Other mutations in Zfp866 |
| Allele | Source | Chr | Coord | Type | Predicted Effect | PPH Score |
|---|
|
IGL02755:Zfp866
|
APN |
8 |
70,219,290 (GRCm39) |
critical splice donor site |
probably null |
|
|
R0238:Zfp866
|
UTSW |
8 |
70,219,365 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R1226:Zfp866
|
UTSW |
8 |
70,218,940 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R1388:Zfp866
|
UTSW |
8 |
70,218,834 (GRCm39) |
missense |
probably benign |
0.16 |
|
R5129:Zfp866
|
UTSW |
8 |
70,220,359 (GRCm39) |
splice site |
probably null |
|
|
R5204:Zfp866
|
UTSW |
8 |
70,218,690 (GRCm39) |
missense |
probably damaging |
0.96 |
|
R5225:Zfp866
|
UTSW |
8 |
70,218,091 (GRCm39) |
missense |
possibly damaging |
0.93 |
|
R5504:Zfp866
|
UTSW |
8 |
70,218,341 (GRCm39) |
missense |
probably benign |
0.07 |
|
R5912:Zfp866
|
UTSW |
8 |
70,218,934 (GRCm39) |
missense |
probably benign |
0.03 |
|
R6046:Zfp866
|
UTSW |
8 |
70,218,373 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R6939:Zfp866
|
UTSW |
8 |
70,218,871 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R7033:Zfp866
|
UTSW |
8 |
70,218,491 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R7111:Zfp866
|
UTSW |
8 |
70,219,221 (GRCm39) |
missense |
probably benign |
0.05 |
|
R7351:Zfp866
|
UTSW |
8 |
70,218,547 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R7458:Zfp866
|
UTSW |
8 |
70,218,202 (GRCm39) |
nonsense |
probably null |
|
|
R8098:Zfp866
|
UTSW |
8 |
70,218,628 (GRCm39) |
missense |
probably benign |
0.02 |
|
R8755:Zfp866
|
UTSW |
8 |
70,219,381 (GRCm39) |
missense |
possibly damaging |
0.78 |
|
R9575:Zfp866
|
UTSW |
8 |
70,219,288 (GRCm39) |
missense |
probably damaging |
0.98 |
|
| Predicted Primers |
PCR Primer
(F):5'- TCAAGGTGTAGGTCAGACACTGCTC -3'
(R):5'- AGGTGGCGTGACTAACGCTGTG -3'
Sequencing Primer
(F):5'- cctccccagagcaataatctaatac -3'
(R):5'- atgagggcagagggcag -3'
|
| Posted On |
2013-11-18 |
Incidental Mutation