Mutagenetix > Incidental Mutation

Incidental Mutation 'R1076:Ang4'

85666

Institutional Source

Beutler Lab

Gene Symbol

Ang4

Ensembl Gene

ENSMUSG00000060615

Gene Name

angiogenin, ribonuclease A family, member 4

Synonyms

Rnase5d

MMRRC Submission

039162-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.052) question?

Stock #

R1076 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

52001349-52011047 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 52001759 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Lysine to Arginine at position 63 (K63R)

Ref Sequence

ENSEMBL: ENSMUSP00000073525 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000073860]

AlphaFold

Q3TMQ6

PDB Structure

Biological and Structural Features of Murine Angiogenin-4, an Angiogenic Protein [X-RAY DIFFRACTION]

Predicted Effect

probably damaging
Transcript: ENSMUST00000073860
AA Change: K63R

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000073525
Gene: ENSMUSG00000060615
AA Change: K63R

Domain	Start	End	E-Value	Type
low complexity region	5	21	N/A	INTRINSIC
RNAse_Pc	25	141	5.34e-61	SMART

Meta Mutation Damage Score

0.7327

Coding Region Coverage

1x: 99.4%
3x: 98.8%
10x: 97.4%
20x: 95.1%

Validation Efficiency

100% (39/39)

Allele List at MGI

Other mutations in this stock

Total: 35 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Ankrd50	T	C	3: 38,509,071 (GRCm39)	N176D	probably damaging	Het
Apbb1	A	T	7: 105,223,062 (GRCm39)	L183Q	probably benign	Het
Cdh17	T	C	4: 11,795,581 (GRCm39)	V387A	probably benign	Het
Cldn4	A	G	5: 134,975,191 (GRCm39)	S137P	probably damaging	Het
Cnn1	A	T	9: 22,019,165 (GRCm39)	Q157L	probably damaging	Het
Csn1s1	A	T	5: 87,824,242 (GRCm39)		probably null	Het
Dennd3	C	T	15: 73,412,582 (GRCm39)	H415Y	probably damaging	Het
Dnpep	A	G	1: 75,292,582 (GRCm39)		probably benign	Het
Dram1	C	A	10: 88,161,246 (GRCm39)	V208L	probably damaging	Het
Elovl2	A	G	13: 41,343,583 (GRCm39)	V115A	possibly damaging	Het
Fryl	C	T	5: 73,282,016 (GRCm39)		probably benign	Het
Gsap	A	G	5: 21,492,692 (GRCm39)	T707A	possibly damaging	Het
Hsh2d	G	A	8: 72,954,304 (GRCm39)	D229N	probably benign	Het
Ktn1	T	A	14: 47,932,095 (GRCm39)	M674K	probably damaging	Het
Larp4	A	G	15: 99,895,311 (GRCm39)	T295A	probably benign	Het
Lrp1	T	C	10: 127,399,666 (GRCm39)		probably benign	Het
Lypd10	A	G	7: 24,413,167 (GRCm39)	K162R	probably benign	Het
Macf1	T	C	4: 123,279,391 (GRCm39)	D3870G	probably damaging	Het
Mctp2	T	G	7: 71,835,615 (GRCm39)		probably null	Het
Nbn	A	T	4: 15,970,719 (GRCm39)		probably null	Het
Neb	A	G	2: 52,094,904 (GRCm39)	Y4883H	probably damaging	Het
Nsun6	A	T	2: 15,014,283 (GRCm39)	C286S	probably benign	Het
Pabpc4	T	A	4: 123,186,701 (GRCm39)	D307E	possibly damaging	Het
Pik3cg	G	A	12: 32,245,713 (GRCm39)		probably benign	Het
Ptpdc1	C	T	13: 48,740,286 (GRCm39)	E382K	probably damaging	Het
Serpina3k	A	G	12: 104,307,253 (GRCm39)	T162A	probably benign	Het
Sis	T	C	3: 72,841,431 (GRCm39)	T795A	probably damaging	Het
Skint8	T	A	4: 111,784,416 (GRCm39)	V14E	probably damaging	Het
Slc2a1	T	G	4: 118,991,645 (GRCm39)	M351R	probably damaging	Het
Slc41a3	G	A	6: 90,621,142 (GRCm39)	C394Y	probably benign	Het
Srp54b	T	C	12: 55,302,313 (GRCm39)		probably benign	Het
Ulk2	G	A	11: 61,710,135 (GRCm39)	H358Y	probably damaging	Het
Utp20	A	T	10: 88,608,405 (GRCm39)	I1544N	possibly damaging	Het
Utp20	A	G	10: 88,608,321 (GRCm39)	M1572T	probably benign	Het
Zbtb14	C	A	17: 69,695,497 (GRCm39)	F398L	probably damaging	Het

Other mutations in Ang4

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01363:Ang4	APN	14	52,001,636 (GRCm39)	missense	probably benign	0.11
IGL01393:Ang4	APN	14	52,001,670 (GRCm39)	missense	probably benign	0.00
IGL02023:Ang4	APN	14	52,001,511 (GRCm39)	utr 3 prime	probably benign
IGL02645:Ang4	APN	14	52,001,804 (GRCm39)	missense	probably damaging	1.00
R0023:Ang4	UTSW	14	52,001,860 (GRCm39)	nonsense	probably null
R0834:Ang4	UTSW	14	52,001,725 (GRCm39)	missense	probably benign
R2258:Ang4	UTSW	14	52,001,955 (GRCm39)	start gained	probably benign
R4524:Ang4	UTSW	14	52,001,605 (GRCm39)	nonsense	probably null
R4981:Ang4	UTSW	14	52,001,829 (GRCm39)	missense	probably benign	0.11
R5929:Ang4	UTSW	14	52,001,708 (GRCm39)	missense	probably damaging	1.00
R7840:Ang4	UTSW	14	52,001,529 (GRCm39)	missense	probably damaging	1.00
R8879:Ang4	UTSW	14	52,001,943 (GRCm39)	missense	probably benign	0.03
Z1176:Ang4	UTSW	14	52,001,605 (GRCm39)	nonsense	probably null

Predicted Primers

PCR Primer
(F):5'- TCCCCTGGGATACAGCAAGAAGAG -3'
(R):5'- TTGGCAGGAATCACTGTTGGAAGAG -3'

Sequencing Primer
(F):5'- CTGGGAACTAGTGATCTTCCAGAC -3'
(R):5'- GACAATGAGCCCATGTCCTTTG -3'

Posted On

2013-11-18