Mutagenetix > Incidental Mutation

Incidental Mutation 'R1077:Cdc25c'

85708

Institutional Source

Beutler Lab

Gene Symbol

Cdc25c

Ensembl Gene

ENSMUSG00000044201

Gene Name

cell division cycle 25C

Synonyms

Cdc25

MMRRC Submission

039163-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R1077 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

34866046-34884586 bp(-) (GRCm39)

Type of Mutation

splice site

DNA Base Change (assembly)

A to T at 34882026 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000055427 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000060710]

AlphaFold

P48967

Predicted Effect

probably benign
Transcript: ENSMUST00000060710

SMART Domains

Protein: ENSMUSP00000055427
Gene: ENSMUSG00000044201

Domain	Start	End	E-Value	Type
low complexity region	246	257	N/A	INTRINSIC
RHOD	284	398	3.71e-21	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000181453

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000181641

Coding Region Coverage

1x: 99.3%
3x: 98.8%
10x: 97.3%
20x: 94.6%

Validation Efficiency

100% (40/40)

MGI Phenotype

FUNCTION: This gene encodes a dual specificity phosphatase that dephosphorylates cyclin B-bound Cdk1 to trigger entry into mitosis. [provided by RefSeq, Dec 2014]
PHENOTYPE: Mice homozygous for a targeted null mutation exhibit no discernable phenotype; mice are viable and fertile with normal T and B lymphocyte development and proliferative responses. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 40 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4921528I07Rik	T	C	9: 114,130,770 (GRCm39)		noncoding transcript	Het
Apob	A	G	12: 8,056,017 (GRCm39)	K1500E	probably benign	Het
Atp8b1	A	T	18: 64,706,333 (GRCm39)	Y225*	probably null	Het
Ceacam15	T	C	7: 16,406,000 (GRCm39)	N184D	probably benign	Het
Cntln	T	C	4: 84,914,716 (GRCm39)	S508P	probably damaging	Het
Defa41	T	A	8: 21,692,449 (GRCm39)	F90Y	probably benign	Het
Dhx34	A	G	7: 15,952,293 (GRCm39)	S111P	probably damaging	Het
Dst	A	G	1: 34,203,248 (GRCm39)	E759G	probably damaging	Het
Fis1	G	A	5: 136,994,000 (GRCm39)	A28T	probably damaging	Het
Fsd1	T	C	17: 56,297,542 (GRCm39)		probably null	Het
Grk6	T	C	13: 55,602,340 (GRCm39)		probably null	Het
Il23r	A	T	6: 67,450,794 (GRCm39)	H228Q	probably benign	Het
Kcnh4	C	T	11: 100,643,164 (GRCm39)	V368I	possibly damaging	Het
Kdr	T	C	5: 76,116,891 (GRCm39)	E728G	probably damaging	Het
Krt33a	T	A	11: 99,906,763 (GRCm39)	M71L	probably benign	Het
Lrrc7	T	G	3: 157,866,780 (GRCm39)	D987A	probably damaging	Het
Naalad2	C	T	9: 18,258,802 (GRCm39)	R491Q	probably damaging	Het
Nedd4l	A	T	18: 65,300,570 (GRCm39)		probably benign	Het
Pramel7	A	G	2: 87,321,534 (GRCm39)	L167S	probably damaging	Het
Prkci	A	G	3: 31,104,341 (GRCm39)	D568G	probably damaging	Het
Psg18	G	A	7: 18,085,000 (GRCm39)	T32I	possibly damaging	Het
Ric8b	T	A	10: 84,806,581 (GRCm39)		probably benign	Het
Rnf213	T	C	11: 119,376,824 (GRCm39)		probably benign	Het
Rttn	T	C	18: 89,082,373 (GRCm39)	V1433A	probably damaging	Het
Sbf2	A	T	7: 109,966,379 (GRCm39)		probably benign	Het
Sdk2	C	T	11: 113,729,472 (GRCm39)		silent	Het
Sfta2	T	C	17: 35,961,019 (GRCm39)		probably benign	Het
Slc17a1	T	A	13: 24,062,433 (GRCm39)		probably benign	Het
Slc6a21	G	A	7: 44,937,626 (GRCm39)	C314Y	probably benign	Het
Smpd4	T	C	16: 17,441,833 (GRCm39)	V35A	probably damaging	Het
Sorl1	T	C	9: 41,925,786 (GRCm39)	D1182G	probably damaging	Het
Syne2	A	G	12: 76,088,809 (GRCm39)	I5056V	possibly damaging	Het
Tex14	T	C	11: 87,410,571 (GRCm39)		probably benign	Het
Tex44	A	G	1: 86,354,777 (GRCm39)	T229A	probably benign	Het
Tfap2b	T	A	1: 19,304,373 (GRCm39)	C394*	probably null	Het
Ttk	T	A	9: 83,726,202 (GRCm39)		probably benign	Het
Vmn2r26	G	A	6: 124,030,872 (GRCm39)	V536I	probably benign	Het
Wac	A	G	18: 7,921,916 (GRCm39)	T553A	probably damaging	Het
Wdcp	T	A	12: 4,900,685 (GRCm39)	H180Q	probably damaging	Het
Wdr33	A	G	18: 31,968,514 (GRCm39)	H235R	probably benign	Het

Other mutations in Cdc25c

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00773:Cdc25c	APN	18	34,880,294 (GRCm39)	missense	probably benign	0.45
IGL01357:Cdc25c	APN	18	34,867,910 (GRCm39)	splice site	probably null
IGL02122:Cdc25c	APN	18	34,877,038 (GRCm39)	missense	probably benign	0.03
R0053:Cdc25c	UTSW	18	34,868,488 (GRCm39)	missense	probably benign	0.16
R0053:Cdc25c	UTSW	18	34,868,488 (GRCm39)	missense	probably benign	0.16
R1679:Cdc25c	UTSW	18	34,880,348 (GRCm39)	missense	probably damaging	1.00
R2036:Cdc25c	UTSW	18	34,871,292 (GRCm39)	missense	probably damaging	1.00
R2051:Cdc25c	UTSW	18	34,871,292 (GRCm39)	missense	probably damaging	1.00
R2077:Cdc25c	UTSW	18	34,871,292 (GRCm39)	missense	probably damaging	1.00
R2511:Cdc25c	UTSW	18	34,871,292 (GRCm39)	missense	probably damaging	1.00
R5304:Cdc25c	UTSW	18	34,883,864 (GRCm39)	missense	possibly damaging	0.71
R5604:Cdc25c	UTSW	18	34,866,701 (GRCm39)	missense	probably damaging	1.00
R7833:Cdc25c	UTSW	18	34,880,296 (GRCm39)	missense	probably benign	0.17
R7919:Cdc25c	UTSW	18	34,868,429 (GRCm39)	missense	probably damaging	0.99
R8193:Cdc25c	UTSW	18	34,882,675 (GRCm39)	splice site	probably null
R8710:Cdc25c	UTSW	18	34,882,666 (GRCm39)	critical splice acceptor site	probably benign
R8960:Cdc25c	UTSW	18	34,866,329 (GRCm39)	missense	possibly damaging	0.90

Predicted Primers

PCR Primer
(F):5'- ACTTGAGCGTGTACAGAGTCTACATAGG -3'
(R):5'- GCCTTGACCTTTCGAATCTTAGCAGTG -3'

Sequencing Primer
(F):5'- GATAACATCCCTGTGATTCATACAGC -3'
(R):5'- cctcagaagccagaagaagaac -3'

Posted On

2013-11-18