Mutagenetix > Incidental Mutation

Incidental Mutation 'R1079:Lzts2'

85809

Institutional Source

Beutler Lab

Gene Symbol

Lzts2

Ensembl Gene

ENSMUSG00000035342

Gene Name

leucine zipper, putative tumor suppressor 2

Synonyms

MMRRC Submission

039165-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.126) question?

Stock #

R1079 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

45003615-45015554 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 45011983 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Asparagine to Lysine at position 137 (N137K)

Ref Sequence

ENSEMBL: ENSMUSP00000137571 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000039016] [ENSMUST00000145391] [ENSMUST00000169459] [ENSMUST00000178087] [ENSMUST00000179108]

AlphaFold

Q91YU6

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000038901

Predicted Effect

probably damaging
Transcript: ENSMUST00000039016
AA Change: N137K

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000045478
Gene: ENSMUSG00000035342
AA Change: N137K

Domain	Start	End	E-Value	Type
low complexity region	99	105	N/A	INTRINSIC
low complexity region	187	199	N/A	INTRINSIC
low complexity region	242	265	N/A	INTRINSIC
low complexity region	267	321	N/A	INTRINSIC
low complexity region	375	407	N/A	INTRINSIC
low complexity region	412	423	N/A	INTRINSIC
Pfam:Fez1	441	639	4.2e-82	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000145391

SMART Domains

Protein: ENSMUSP00000119002
Gene: ENSMUSG00000074818

Domain	Start	End	E-Value	Type
low complexity region	12	35	N/A	INTRINSIC
PDZ	95	167	3.51e-19	SMART
PDZ	220	292	2.47e-14	SMART
low complexity region	319	344	N/A	INTRINSIC
low complexity region	442	459	N/A	INTRINSIC
low complexity region	521	533	N/A	INTRINSIC
low complexity region	724	744	N/A	INTRINSIC
low complexity region	768	809	N/A	INTRINSIC
low complexity region	812	824	N/A	INTRINSIC
PDZ	866	947	1.96e-8	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000169459

SMART Domains

Protein: ENSMUSP00000133273
Gene: ENSMUSG00000074818

Domain	Start	End	E-Value	Type
low complexity region	12	35	N/A	INTRINSIC
PDZ	95	167	3.51e-19	SMART
PDZ	220	292	2.47e-14	SMART
low complexity region	319	344	N/A	INTRINSIC
low complexity region	442	459	N/A	INTRINSIC
low complexity region	521	533	N/A	INTRINSIC
low complexity region	724	744	N/A	INTRINSIC
low complexity region	768	809	N/A	INTRINSIC
low complexity region	812	824	N/A	INTRINSIC
PDZ	866	947	1.96e-8	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000178087
AA Change: N137K

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000136405
Gene: ENSMUSG00000035342
AA Change: N137K

Domain	Start	End	E-Value	Type
low complexity region	99	105	N/A	INTRINSIC
low complexity region	187	199	N/A	INTRINSIC
low complexity region	242	265	N/A	INTRINSIC
low complexity region	267	321	N/A	INTRINSIC
low complexity region	375	407	N/A	INTRINSIC
low complexity region	412	423	N/A	INTRINSIC
Pfam:Fez1	441	638	2.2e-66	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000179108
AA Change: N137K

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000137571
Gene: ENSMUSG00000035342
AA Change: N137K

Domain	Start	End	E-Value	Type
low complexity region	99	105	N/A	INTRINSIC
low complexity region	187	199	N/A	INTRINSIC
low complexity region	242	265	N/A	INTRINSIC
low complexity region	267	321	N/A	INTRINSIC
low complexity region	375	407	N/A	INTRINSIC
low complexity region	412	423	N/A	INTRINSIC
Pfam:Fez1	441	639	4.2e-82	PFAM

Coding Region Coverage

1x: 99.4%
3x: 98.9%
10x: 97.6%
20x: 95.6%

Validation Efficiency

98% (46/47)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene belongs to the leucine zipper tumor suppressor family of proteins, which function in transcription regulation and cell cycle control. This family member can repress beta-catenin-mediated transcriptional activation and is a negative regulator of the Wnt signaling pathway. It negatively regulates microtubule severing at centrosomes, and is necessary for central spindle formation and cytokinesis completion. It is implicated in cancer, where it may inhibit cell proliferation and decrease susceptibility to tumor development. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Dec 2015]
PHENOTYPE: Mice homozygous for a null mutation display defects in urinary tract development. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 47 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2200002J24Rik	T	C	7: 30,399,209 (GRCm39)	M1T	probably null	Het
Adam25	T	C	8: 41,208,513 (GRCm39)	V593A	possibly damaging	Het
Agrn	C	T	4: 156,261,682 (GRCm39)	C536Y	probably damaging	Het
Amigo3	T	C	9: 107,931,051 (GRCm39)	M158T	probably benign	Het
Arsa	G	A	15: 89,358,428 (GRCm39)		probably benign	Het
Baz1a	T	A	12: 54,941,785 (GRCm39)	I1474L	possibly damaging	Het
Cfap144	A	G	11: 58,692,620 (GRCm39)	Y36H	probably benign	Het
Cfap65	A	G	1: 74,944,872 (GRCm39)	V1455A	probably damaging	Het
Cfap65	G	A	1: 74,941,606 (GRCm39)	A1776V	probably damaging	Het
Cnot6	G	T	11: 49,575,930 (GRCm39)	D176E	probably benign	Het
Crot	A	T	5: 9,043,504 (GRCm39)		probably null	Het
Cryba2	A	T	1: 74,929,717 (GRCm39)	V140E	probably damaging	Het
Dennd4b	G	A	3: 90,178,485 (GRCm39)	R516K	probably benign	Het
Dst	C	A	1: 34,225,944 (GRCm39)	T1697N	possibly damaging	Het
Eftud2	G	T	11: 102,730,870 (GRCm39)	Y837*	probably null	Het
Evpl	G	T	11: 116,120,894 (GRCm39)	T447K	possibly damaging	Het
Fndc3a	A	T	14: 72,827,247 (GRCm39)	M146K	possibly damaging	Het
Folh1	G	T	7: 86,421,089 (GRCm39)	T80K	probably damaging	Het
Gm4922	T	A	10: 18,660,086 (GRCm39)	Y212F	probably damaging	Het
Gtf2i	T	G	5: 134,271,748 (GRCm39)		probably benign	Het
Hipk3	A	G	2: 104,302,043 (GRCm39)	F50L	probably benign	Het
Ifit1bl2	T	A	19: 34,596,885 (GRCm39)	T244S	probably benign	Het
Ikzf2	T	C	1: 69,578,264 (GRCm39)	D341G	possibly damaging	Het
Kif3a	G	C	11: 53,461,408 (GRCm39)	V17L	possibly damaging	Het
Lgr6	C	T	1: 134,921,748 (GRCm39)	A199T	probably damaging	Het
Mphosph8	T	A	14: 56,911,716 (GRCm39)	D246E	probably damaging	Het
Myh14	T	C	7: 44,279,426 (GRCm39)	E918G	probably damaging	Het
N6amt1	T	C	16: 87,153,086 (GRCm39)	V52A	probably damaging	Het
Npr2	A	C	4: 43,643,654 (GRCm39)	T561P	probably damaging	Het
Nudt12	G	A	17: 59,318,032 (GRCm39)		probably benign	Het
Or10ad1	A	C	15: 98,106,223 (GRCm39)	V14G	probably damaging	Het
Or5w8	A	G	2: 87,687,699 (GRCm39)	Y60C	probably damaging	Het
Or6n2	A	T	1: 173,897,032 (GRCm39)	H56L	possibly damaging	Het
Or8k22	T	A	2: 86,163,185 (GRCm39)	R172W	probably damaging	Het
Pan2	A	G	10: 128,154,107 (GRCm39)	T1050A	probably damaging	Het
Rad17	G	T	13: 100,770,407 (GRCm39)	D213E	probably benign	Het
Sall2	T	A	14: 52,550,660 (GRCm39)	H843L	probably benign	Het
Sdk2	C	T	11: 113,729,472 (GRCm39)		silent	Het
Sema3e	A	T	5: 14,275,669 (GRCm39)	N258I	probably benign	Het
Siglec1	G	A	2: 130,921,297 (GRCm39)	R625*	probably null	Het
Slc15a3	T	C	19: 10,833,344 (GRCm39)	S454P	probably benign	Het
Sp110	G	A	1: 85,516,825 (GRCm39)		probably benign	Het
Spatc1l	T	C	10: 76,399,741 (GRCm39)	S88P	probably damaging	Het
Ssh3	A	C	19: 4,316,577 (GRCm39)	L143R	probably damaging	Het
Ttn	C	T	2: 76,587,340 (GRCm39)		probably benign	Het
Vps35	A	G	8: 86,005,683 (GRCm39)	L306S	probably damaging	Het
Zfp729a	T	C	13: 67,767,794 (GRCm39)	I812V	possibly damaging	Het

Other mutations in Lzts2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02115:Lzts2	APN	19	45,014,809 (GRCm39)	unclassified	probably benign
R0053:Lzts2	UTSW	19	45,014,746 (GRCm39)	unclassified	probably benign
R0135:Lzts2	UTSW	19	45,014,626 (GRCm39)	unclassified	probably benign
R0433:Lzts2	UTSW	19	45,010,115 (GRCm39)	missense	possibly damaging	0.87
R1459:Lzts2	UTSW	19	45,009,893 (GRCm39)	missense	probably damaging	1.00
R2869:Lzts2	UTSW	19	45,012,534 (GRCm39)	nonsense	probably null
R2869:Lzts2	UTSW	19	45,012,534 (GRCm39)	nonsense	probably null
R4450:Lzts2	UTSW	19	45,012,032 (GRCm39)	nonsense	probably null
R5806:Lzts2	UTSW	19	45,014,806 (GRCm39)	unclassified	probably benign
R6958:Lzts2	UTSW	19	45,012,582 (GRCm39)	unclassified	probably benign
R7598:Lzts2	UTSW	19	45,012,272 (GRCm39)	nonsense	probably null
R9398:Lzts2	UTSW	19	45,013,208 (GRCm39)	missense	unknown

Predicted Primers

PCR Primer
(F):5'- GGCTTGGTATGACACACCTGTAATCTTG -3'
(R):5'- GGGTTTGTCAGCAGCAGAGGAG -3'

Sequencing Primer
(F):5'- atctgcctgcctctgcc -3'
(R):5'- aggaggaagaggaagaggag -3'

Posted On

2013-11-18