Mutagenetix > Incidental Mutation

Incidental Mutation 'R0714:Or9g4b'

85890

Institutional Source

Beutler Lab

Gene Symbol

Or9g4b

Ensembl Gene

ENSMUSG00000033850

Gene Name

olfactory receptor family 9 subfamily G member 4B

Synonyms

GA_x6K02T2Q125-47264151-47265089, Olfr1015, MOR213-3

MMRRC Submission

038897-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.244) question?

Stock #

R0714 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

85615818-85616833 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 85616743 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Aspartic acid to Glycine at position 296 (D296G)

Ref Sequence

ENSEMBL: ENSMUSP00000148957 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000047870] [ENSMUST00000215945]

AlphaFold

Q7TR94

Predicted Effect

probably damaging
Transcript: ENSMUST00000047870
AA Change: D296G

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000046301
Gene: ENSMUSG00000033850
AA Change: D296G

Domain	Start	End	E-Value	Type
Pfam:7tm_4	31	308	4.5e-49	PFAM
Pfam:7tm_1	41	290	4.2e-19	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000214726

Predicted Effect

probably damaging
Transcript: ENSMUST00000215945
AA Change: D296G

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

Meta Mutation Damage Score

0.5332

Coding Region Coverage

1x: 99.4%
3x: 98.9%
10x: 97.6%
20x: 95.5%

Validation Efficiency

100% (52/52)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 50 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abhd17b	G	A	19: 21,655,973 (GRCm39)	R85H	probably damaging	Het
Adamts13	A	G	2: 26,876,997 (GRCm39)		probably benign	Het
Alb	T	C	5: 90,610,665 (GRCm39)	S82P	possibly damaging	Het
Arhgap10	G	A	8: 78,078,316 (GRCm39)		probably benign	Het
Aspscr1	T	A	11: 120,594,493 (GRCm39)		probably null	Het
Capn3	A	T	2: 120,322,361 (GRCm39)	Q359L	probably benign	Het
Ccdc110	A	T	8: 46,396,047 (GRCm39)	D646V	possibly damaging	Het
Ccr2	G	A	9: 123,905,966 (GRCm39)	G82D	probably benign	Het
Col6a4	A	G	9: 105,895,102 (GRCm39)		probably benign	Het
Dhx29	T	C	13: 113,064,499 (GRCm39)	V58A	possibly damaging	Het
Dhx35	C	A	2: 158,686,103 (GRCm39)	Q593K	probably benign	Het
Dmd	T	C	X: 83,353,503 (GRCm39)	L2240P	probably benign	Het
Emc7	A	G	2: 112,293,277 (GRCm39)	N162S	possibly damaging	Het
Exoc7	A	T	11: 116,184,120 (GRCm39)	N483K	probably benign	Het
Fbxo34	T	C	14: 47,767,486 (GRCm39)	V282A	probably damaging	Het
Fndc3c1	A	T	X: 105,468,972 (GRCm39)	Y1087*	probably null	Het
Kat2a	A	G	11: 100,602,178 (GRCm39)	V192A	probably damaging	Het
Larp7	T	C	3: 127,340,833 (GRCm39)	D64G	probably damaging	Het
Lnx1	A	G	5: 74,768,570 (GRCm39)		probably benign	Het
Mib2	C	T	4: 155,743,917 (GRCm39)	G42S	probably damaging	Het
Nckipsd	C	A	9: 108,691,333 (GRCm39)		probably benign	Het
Ndufab1	A	G	7: 121,695,960 (GRCm39)		probably benign	Het
Nedd4	G	A	9: 72,638,728 (GRCm39)		probably benign	Het
Nrsn2	G	A	2: 152,216,042 (GRCm39)	R54*	probably null	Het
Nt5dc3	T	A	10: 86,648,238 (GRCm39)	V171E	probably damaging	Het
Nudt8	A	G	19: 4,052,023 (GRCm39)	*211W	probably null	Het
Nxph4	A	G	10: 127,362,808 (GRCm39)	S28P	probably damaging	Het
Or4f14b	A	G	2: 111,774,898 (GRCm39)	V301A	probably benign	Het
Or8k17	A	C	2: 86,066,498 (GRCm39)	L227R	probably damaging	Het
Pcdhb15	A	G	18: 37,607,674 (GRCm39)	Y302C	probably damaging	Het
Pkdrej	A	G	15: 85,699,712 (GRCm39)	S2075P	possibly damaging	Het
Sdhc	A	T	1: 170,957,488 (GRCm39)		probably benign	Het
Sidt2	A	G	9: 45,858,358 (GRCm39)		probably benign	Het
Sik2	T	C	9: 50,818,736 (GRCm39)	M413V	probably benign	Het
Slc5a4b	A	G	10: 75,917,341 (GRCm39)	F232L	probably benign	Het
Slx1b	A	T	7: 126,291,620 (GRCm39)	I148N	probably damaging	Het
Spag17	C	G	3: 99,987,472 (GRCm39)	S1587R	probably damaging	Het
St13	T	C	15: 81,267,228 (GRCm39)	D74G	probably benign	Het
St7l	G	A	3: 104,782,244 (GRCm39)	R207H	probably benign	Het
Syne2	AGAGTGAG	AGAGTGAGTGAG	12: 76,144,734 (GRCm39)		probably null	Het
Tacc3	T	C	5: 33,828,741 (GRCm39)		probably benign	Het
Tbx22	G	A	X: 106,728,731 (GRCm39)	V421I	probably benign	Het
Tmc3	G	A	7: 83,265,969 (GRCm39)	A705T	possibly damaging	Het
Tmem130	C	T	5: 144,674,619 (GRCm39)	V369M	probably damaging	Het
Tonsl	T	C	15: 76,517,921 (GRCm39)		probably benign	Het
Trpm6	T	A	19: 18,815,451 (GRCm39)	I1179N	possibly damaging	Het
Ttc13	A	T	8: 125,401,105 (GRCm39)	S624T	probably damaging	Het
Utp23	T	G	15: 51,745,665 (GRCm39)	V55G	possibly damaging	Het
Vps11	A	G	9: 44,270,953 (GRCm39)	V143A	possibly damaging	Het
Vps50	G	T	6: 3,571,105 (GRCm39)	V618F	probably benign	Het

Other mutations in Or9g4b

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00902:Or9g4b	APN	2	85,616,461 (GRCm39)	missense	probably benign	0.21
IGL01716:Or9g4b	APN	2	85,616,487 (GRCm39)	missense	probably damaging	0.97
IGL03196:Or9g4b	APN	2	85,616,365 (GRCm39)	missense	possibly damaging	0.63
IGL03374:Or9g4b	APN	2	85,616,053 (GRCm39)	missense	probably damaging	1.00
R0329:Or9g4b	UTSW	2	85,616,147 (GRCm39)	nonsense	probably null
R0330:Or9g4b	UTSW	2	85,616,147 (GRCm39)	nonsense	probably null
R0965:Or9g4b	UTSW	2	85,616,643 (GRCm39)	missense	probably damaging	1.00
R1078:Or9g4b	UTSW	2	85,616,437 (GRCm39)	missense	possibly damaging	0.53
R3826:Or9g4b	UTSW	2	85,616,559 (GRCm39)	nonsense	probably null
R5031:Or9g4b	UTSW	2	85,616,062 (GRCm39)	nonsense	probably null
R5239:Or9g4b	UTSW	2	85,616,002 (GRCm39)	missense	probably damaging	1.00
R6120:Or9g4b	UTSW	2	85,616,685 (GRCm39)	missense	probably damaging	1.00
R6177:Or9g4b	UTSW	2	85,616,004 (GRCm39)	missense	probably damaging	0.99
R6726:Or9g4b	UTSW	2	85,615,906 (GRCm39)	missense	possibly damaging	0.51
R6954:Or9g4b	UTSW	2	85,616,726 (GRCm39)	nonsense	probably null
R7766:Or9g4b	UTSW	2	85,616,002 (GRCm39)	missense	probably damaging	1.00
R8193:Or9g4b	UTSW	2	85,616,305 (GRCm39)	missense	probably benign	0.34
R8245:Or9g4b	UTSW	2	85,616,119 (GRCm39)	missense	probably benign	0.02
R8339:Or9g4b	UTSW	2	85,615,876 (GRCm39)	missense	probably damaging	0.98
R9272:Or9g4b	UTSW	2	85,616,088 (GRCm39)	missense	probably benign	0.09
Z1176:Or9g4b	UTSW	2	85,616,464 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TCCGCTCAGCCTCAGGAAGAC -3'
(R):5'- CCAGGTGCAGAAAACAAAAGTTGTACAC -3'

Sequencing Primer
(F):5'- CTCAGCCTCAGGAAGACGAAAG -3'
(R):5'- CGAAGCAAAATCCATCATTTTGG -3'

Posted On

2013-11-18