Mutagenetix > Incidental Mutation

Incidental Mutation 'IGL01635:Gstt1'

92958

Institutional Source

Australian Phenomics Network (link to record)

Gene Symbol

Gstt1

Ensembl Gene

ENSMUSG00000001663

Gene Name

glutathione S-transferase, theta 1

Synonyms

Gstt1-1

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.182) question?

Stock #

IGL01635

Quality Score

Status

Chromosome

Chromosomal Location

75619647-75634418 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

G to T at 75629951 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Lysine at position 54 (T54K)

Ref Sequence

ENSEMBL: ENSMUSP00000117130 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000001713] [ENSMUST00000120177] [ENSMUST00000139724]

AlphaFold

Q64471

Predicted Effect

probably damaging
Transcript: ENSMUST00000001713
AA Change: T104K

PolyPhen 2 Score 0.986 (Sensitivity: 0.74; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000001713
Gene: ENSMUSG00000001663
AA Change: T104K

Domain	Start	End	E-Value	Type
Pfam:GST_N	1	76	7.5e-13	PFAM
Pfam:GST_N_3	5	83	1.5e-11	PFAM
Pfam:GST_N_2	12	77	1.1e-7	PFAM
Pfam:GST_C_3	88	200	6.2e-10	PFAM
Pfam:GST_C	111	201	3.7e-10	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000120177
AA Change: T96K

PolyPhen 2 Score 0.992 (Sensitivity: 0.70; Specificity: 0.97)

SMART Domains

Protein: ENSMUSP00000113839
Gene: ENSMUSG00000001663
AA Change: T96K

Domain	Start	End	E-Value	Type
Pfam:GST_N	8	68	5.8e-11	PFAM
Pfam:GST_N_3	20	77	7.3e-9	PFAM
Pfam:GST_C	103	193	5e-11	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000139724
AA Change: T54K

PolyPhen 2 Score 0.995 (Sensitivity: 0.68; Specificity: 0.97)

SMART Domains

Protein: ENSMUSP00000117130
Gene: ENSMUSG00000001663
AA Change: T54K

Domain	Start	End	E-Value	Type
Pfam:GST_N	1	26	4.6e-7	PFAM
Pfam:GST_C	61	126	8.1e-7	PFAM

Coding Region Coverage

Validation Efficiency

MGI Phenotype

PHENOTYPE: Mis homozygous for disruption of this gene appear to be normal and fertile with the exception of a reduced ability to clear 11,2-bis(2-chloroethyl)-1-nitrosourea from the plasma after a single i.p. dose. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 29 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abat	A	G	16: 8,431,910 (GRCm39)	I396V	probably benign	Het
Bag2	T	A	1: 33,786,013 (GRCm39)	N103I	possibly damaging	Het
Cfap95	T	C	19: 23,536,379 (GRCm39)	Y176C	probably damaging	Het
Chd1	T	C	17: 17,598,858 (GRCm39)	S107P	probably damaging	Het
Chd3	A	T	11: 69,252,076 (GRCm39)		probably benign	Het
Cytip	T	C	2: 58,038,243 (GRCm39)	E106G	probably damaging	Het
Dcp1b	T	A	6: 119,183,498 (GRCm39)	I195N	probably damaging	Het
Ddhd1	C	A	14: 45,867,037 (GRCm39)	V322F	probably null	Het
Esrrg	A	T	1: 187,930,797 (GRCm39)	D296V	probably damaging	Het
F5	A	G	1: 164,035,427 (GRCm39)	K1867R	probably benign	Het
Gm10717	C	A	9: 3,025,511 (GRCm39)	A32D	probably damaging	Het
Gm10717	C	A	9: 3,025,506 (GRCm39)	F30L	possibly damaging	Het
Ighmbp2	C	T	19: 3,317,265 (GRCm39)	V536I	possibly damaging	Het
Mphosph8	A	T	14: 56,910,003 (GRCm39)	R119W	probably damaging	Het
Negr1	A	G	3: 156,267,929 (GRCm39)	D33G	probably benign	Het
Or2l13b	A	G	16: 19,348,845 (GRCm39)	V275A	probably benign	Het
Or7d10	A	T	9: 19,831,780 (GRCm39)	I92L	probably damaging	Het
Plbd2	T	C	5: 120,637,114 (GRCm39)	D116G	probably damaging	Het
Pold1	A	T	7: 44,185,401 (GRCm39)	V733E	probably damaging	Het
Prorsd1	T	A	11: 29,463,467 (GRCm39)	K98N	probably benign	Het
Rasal2	G	T	1: 156,991,394 (GRCm39)	L611I	probably damaging	Het
Rffl	G	A	11: 82,703,378 (GRCm39)	R147C	probably benign	Het
Rpl27rt	A	T	18: 34,870,795 (GRCm39)	M110L	probably benign	Het
Sdad1	A	G	5: 92,445,019 (GRCm39)	M315T	probably damaging	Het
Shprh	T	A	10: 11,045,763 (GRCm39)	Y861*	probably null	Het
Six4	G	A	12: 73,155,971 (GRCm39)	A324V	probably benign	Het
Stab2	T	C	10: 86,816,992 (GRCm39)	D135G	probably benign	Het
Tada3	T	C	6: 113,352,973 (GRCm39)	H12R	probably benign	Het
Zap70	A	G	1: 36,810,238 (GRCm39)	D116G	probably damaging	Het

Other mutations in Gstt1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
R1460:Gstt1	UTSW	10	75,620,004 (GRCm39)	missense	probably damaging	0.99
R1646:Gstt1	UTSW	10	75,619,940 (GRCm39)	missense	possibly damaging	0.91
R4603:Gstt1	UTSW	10	75,629,969 (GRCm39)	missense	probably damaging	1.00
R6757:Gstt1	UTSW	10	75,634,217 (GRCm39)	critical splice donor site	probably null
R7896:Gstt1	UTSW	10	75,619,976 (GRCm39)	missense	probably damaging	1.00
R8459:Gstt1	UTSW	10	75,629,943 (GRCm39)	missense	probably damaging	1.00
R9792:Gstt1	UTSW	10	75,634,391 (GRCm39)	unclassified	probably benign
R9795:Gstt1	UTSW	10	75,634,391 (GRCm39)	unclassified	probably benign

Posted On

2013-12-09