|Institutional Source||Beutler Lab|
|Gene Name||5-hydroxytryptamine (serotonin) receptor 5A|
|Is this an essential gene?||Probably non essential (E-score: 0.143)|
|Stock #||R1023 (G1)|
|Chromosomal Location||27842149-27855088 bp(+) (GRCm38)|
|Type of Mutation||missense|
|DNA Base Change (assembly)||A to T at 27842998 bp|
|Amino Acid Change||Threonine to Serine at position 184 (T184S)|
|Ref Sequence||ENSEMBL: ENSMUSP00000038884 (fasta)|
|Gene Model||predicted gene model for transcript(s): [ENSMUST00000036227]|
|Predicted Effect||possibly damaging
AA Change: T184S
PolyPhen 2 Score 0.810 (Sensitivity: 0.84; Specificity: 0.93)
AA Change: T184S
|Meta Mutation Damage Score||0.05|
|Coding Region Coverage||
|Validation Efficiency||100% (42/42)|
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The neurotransmitter serotonin (5-hydroxytryptamine, 5-HT) has been implicated in a wide range of psychiatric conditions and also has vasoconstrictive and vasodilatory effects. The gene described in this record is a member of 5-hydroxytryptamine (serotonin) receptor family and encodes a multi-pass membrane protein that functions as a receptor for 5-hydroxytryptamine and couples to G-proteins. This protein has been shown to function in part through the regulation of intracellular Ca2+ mobilization. [provided by RefSeq, Jul 2008]
PHENOTYPE: The are conflicting reports about mice with homozygous null alleles regarding increased exploratory behavior and an abnormal exploratory behavior response to LSD. [provided by MGI curators]
|Allele List at MGI|
|Other mutations in this stock||
|Other mutations in Htr5a||
(F):5'- ACAGCAATAGCTCTGGACCGCTAC -3'
(R):5'- CGCTGCAAGAAAAGCCTGTGAG -3'
(F):5'- TGGACCGCTACTGGTCAATC -3'
(R):5'- CAAGTGACGTATTTTCTAACGATCC -3'